Restriction map for pF1KSDB0062
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       FauI        
FbaI        FseI        FspI        HaeII       HhaI        
HinP1I      HpaI        KasI        KpnI        KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI         1805  GCGG                 CCGC(-3/-1)
AcyI           58  GGCGTC               G[GA]^CG[CT]C
AfaI          439  GTAC                 GT^AC
AlwNI        1052  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1270  CCTGAGG              CC^T[ACGT]AGG
AvaI         1490  CCCGAG               C^[CT]CG[GA]G
BanI          837  GGCACC               G^G[CT][GA]CC
BbsI         1987  GAAGAC               GAAGAC(2/6)
BciVI        1251  GGATAC               GTATCC(6/5)
BpuAI        1987  GAAGAC               GAAGAC(2/6)
BsaBI         760  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          58  GGCGTC               G[GA]^CG[CT]C
BsaI         1889  GGTCTC               GGTCTC(1/5)
BsmBI          60  CGTCTC               CGTCTC(1/5)
BsmI         1476  GCATTC               GAATGC(1/-1)
BsoBI        1490  CCCGAG               C^[CT]CG[GA]G
BsrDI        1504  CATTGC               GCAATG(2/0)
BstYI         355  GGATCT               [GA]^GATC[CT]
Bsu36I       1270  CCTGAGG              CC^T[ACGT]AGG
DraI         1044  TTTAAA               TTT^AAA
DraII         375  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          235  CCATGG               C^C[GA][CT]GG
EaeI         1021  CGGCCA               [CT]^GGCC[GA]
Eco81I       1270  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      375  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      1474  ATGCAT               ATGCA^T
HapII        1879  CCGG                 C^CGG
HgaI           59  GCGTC                GACGC(5/10)
Hin1I          58  GGCGTC               G[GA]^CG[CT]C
HincII         22  GTTGAC               GT[CT]^[GA]AC
HindII         22  GTTGAC               GT[CT]^[GA]AC
HindIII       513  AAGCTT               A^AGCTT
HpaII        1879  CCGG                 C^CGG
MamI          760  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          355  GGATCT               [GA]^GATC[CT]
MslI         1674  CATTCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1803  CAGCGG               C[AC]G^C[GT]G
MspI         1879  CCGG                 C^CGG
MwoI         1591  GCAGCAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          235  CCATGG               C^CATGG
NdeI          520  CATATG               CA^TATG
NheI         1930  GCTAGC               G^CTAGC
NsiI         1474  ATGCAT               ATGCA^T
NspI         1295  ACATGC               [GA]CATG^[CT]
PpuMI         375  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1838  CTGCAG               CTGCA^G
RsaI          439  GTAC                 GT^AC
SspI          817  AATATT               AAT^ATT
XcmI         1119  CCAAGCTATTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         355  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          355  GGATC                GGATC(4/5)
             1519  GGATC               
AseI          366  ATTAAT               AT^TAAT
              451  ATTAAT              
AsnI          366  ATTAAT               AT^TAAT
              451  ATTAAT              
AvaII         376  GGTCC                G^G[AT]CC
             1116  GGTCC               
BbvI          140  GCAGC                GCAGC(8/12)
             1591  GCAGC               
BpmI          985  CTCCAG               CTGGAG(16/14)
             1301  CTGGAG              
BseRI        1273  GAGGAG               GAGGAG(10/8)
             1514  GAGGAG              
BsmFI        2005  GGGAC                GGGAC(10/14)
              913  GTCCC               
EcoRV         961  GATATC               GAT^ATC
             1399  GATATC              
Fnu4HI        140  GCAGC                GC^[ACGT]GC
             1591  GCAGC               
HaeIII        122  GGCC                 GG^CC
             1022  GGCC                
ItaI          140  GCAGC                GC^[ACGT]GC
             1591  GCAGC               
NlaIV         837  GGCACC               GG[ACGT]^[ACGT]CC
             1724  GGCTCC              
PleI         1210  GACTC                GAGTC(4/5)
             1902  GAGTC               
PshBI         366  ATTAAT               AT^TAAT
              451  ATTAAT              
SexAI         310  ACCTGGT              A^CC[AT]GGT
             1885  ACCTGGT             
TseI          140  GCAGC                G^C[AT]GC
             1591  GCAGC               
XbaI           45  TCTAGA               T^CTAGA
              930  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
FokI        HinfI       HphI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        NdeII       NlaIII      Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com