Restriction map for pF1KSDB0062
 (Linear) (Six-base) MAPSORT of: KIBB0062.seq  Check: 5712  from: 1  to: 2010



KIBB0062.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1140    1753    2010 
   Size:       1140     613     257

  Fragments arranged by size: 

                1140     613     257
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     290    2010 
   Size:        290    1720
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1057    2010 
   Size:       1057     953
 
ApoI r'AATT_y

Cuts at:      0     204     791     856    1172    1381    2010 
   Size:        204     587      65     316     209     629

  Fragments arranged by size: 

                 629     587     316     209     204      65
 
AseI AT'TA_AT

Cuts at:      0     367     452    2010 
   Size:        367      85    1558

  Fragments arranged by size: 

                1558     367      85
 
AvaI C'yCGr_G

Cuts at:      0    1490    2010 
   Size:       1490     520
 
BanI G'GyrC_C

Cuts at:      0     837    2010 
   Size:        837    1173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1994    2010 
   Size:       1994      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1245    2010 
   Size:       1245     765
 
BfrBI ATG'CAT

Cuts at:      0    1476    2010 
   Size:       1476     534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      27    2010 
   Size:         27    1983
 
BmtI G_CTAG'C

Cuts at:      0    1934    2010 
   Size:       1934      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1322    2010 
   Size:        970     352     688

  Fragments arranged by size: 

                 970     688     352
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     308    1850    2010 
   Size:         84     224    1542     160

  Fragments arranged by size: 

                1542     224     160      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1895    2010 
   Size:       1895     115
 
BsaBI GATnn'nnATC

Cuts at:      0     764    2010 
   Size:        764    1246
 
BsaHI Gr'CG_yC

Cuts at:      0      59    2010 
   Size:         59    1951
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     363     393    2010 
   Size:        363      30    1617

  Fragments arranged by size: 

                1617     363      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1288    1529    2010 
   Size:       1288     241     481

  Fragments arranged by size: 

                1288     481     241
 
BseYI C'CCAG_C

Cuts at:      0    1817    1940    2010 
   Size:       1817     123      70

  Fragments arranged by size: 

                1817     123      70
 
BsmI GAATG_Cn'

Cuts at:      0    1476    2010 
   Size:       1476     534
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      66    2010 
   Size:         66    1944
 
BsrDI GCAATG_nn'

Cuts at:      0    1503    2010 
   Size:       1503     507
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1126    1717    1825    2010 
   Size:       1126     591     108     185

  Fragments arranged by size: 

                1126     591     185     108
 
BstYI r'GATC_y

Cuts at:      0     355    2010 
   Size:        355    1655
 
Bsu36I CC'TnA_GG

Cuts at:      0    1271    2010 
   Size:       1271     739
 
BtgI C'CryG_G

Cuts at:      0     235    2010 
   Size:        235    1775
 
DraI TTT'AAA

Cuts at:      0    1046    2010 
   Size:       1046     964
 
EaeI y'GGCC_r

Cuts at:      0    1021    2010 
   Size:       1021     989
 
EarI CTCTTCn'nnn_

Cuts at:      0     794    1159    1898    2010 
   Size:        794     365     739     112

  Fragments arranged by size: 

                 794     739     365     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1140    1322    1753    2010 
   Size:        970     170     182     431     257

  Fragments arranged by size: 

                 970     431     257     182     170
 
EcoO109I rG'GnC_Cy

Cuts at:      0     376    2010 
   Size:        376    1634
 
EcoRV GAT'ATC

Cuts at:      0     963    1401    2010 
   Size:        963     438     609

  Fragments arranged by size: 

                 963     609     438
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1211    1758    1837    2010 
   Size:       1211     547      79     173

  Fragments arranged by size: 

                1211     547     173      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     699     731    2010 
   Size:        699      32    1279

  Fragments arranged by size: 

                1279     699      32
 
HincII GTy'rAC

Cuts at:      0      24    2010 
   Size:         24    1986
 
HindIII A'AGCT_T

Cuts at:      0     513    2010 
   Size:        513    1497
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1505    2010 
   Size:       1505     505
 
MslI CAynn'nnrTG

Cuts at:      0    1678    2010 
   Size:       1678     332
 
MspA1I CmG'CkG

Cuts at:      0    1805    2010 
   Size:       1805     205
 
NcoI C'CATG_G

Cuts at:      0     235    2010 
   Size:        235    1775
 
NdeI CA'TA_TG

Cuts at:      0     521    2010 
   Size:        521    1489
 
NheI G'CTAG_C

Cuts at:      0    1930    2010 
   Size:       1930      80
 
NsiI A_TGCA'T

Cuts at:      0    1478    2010 
   Size:       1478     532
 
NspI r_CATG'y

Cuts at:      0    1299    2010 
   Size:       1299     711
 
PfoI T'CCnGG_A

Cuts at:      0     381    2010 
   Size:        381    1629
 
PpuMI rG'GwC_Cy

Cuts at:      0     376    2010 
   Size:        376    1634
 
PsiI TTA'TAA

Cuts at:      0     736    2010 
   Size:        736    1274
 
PstI C_TGCA'G

Cuts at:      0    1842    2010 
   Size:       1842     168
 
SexAI A'CCwGG_T

Cuts at:      0     310    1885    2010 
   Size:        310    1575     125

  Fragments arranged by size: 

                1575     310     125
 
SfcI C'TryA_G

Cuts at:      0     878    1454    1838    2010 
   Size:        878     576     384     172

  Fragments arranged by size: 

                 878     576     384     172
 
SmlI C'TyrA_G

Cuts at:      0      99     323    1865    2010 
   Size:         99     224    1542     145

  Fragments arranged by size: 

                1542     224     145      99
 
SspI AAT'ATT

Cuts at:      0     819    2010 
   Size:        819    1191
 
StyI C'CwwG_G

Cuts at:      0     235    1024    1509    1956    2010 
   Size:        235     789     485     447      54

  Fragments arranged by size: 

                 789     485     447     235      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     228    1843    2010 
   Size:        228    1615     167

  Fragments arranged by size: 

                1615     228     167
 
TatI w'GTAC_w

Cuts at:      0     438    2010 
   Size:        438    1572
 
XbaI T'CTAG_A

Cuts at:      0      45     930    2010 
   Size:         45     885    1080

  Fragments arranged by size: 

                1080     885      45
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1126    2010 
   Size:       1126     884
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     AseI     AvaI     BanI     BbsI
    BciVI    BfrBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsmI    BsmBI    BsrDI    BstXI
    BstYI   Bsu36I     BtgI     DraI     EaeI     EarI  Eco57MI EcoO109I
    EcoRV     FalI    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I
     NcoI     NdeI     NheI     NsiI     NspI     PfoI    PpuMI     PsiI
     PstI    SexAI     SfcI     SmlI     SspI     StyI    TaqII     TatI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BplI   Bpu10I    BsaAI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI
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