Restriction map for
pF1KSDB0034
(Linear) (Six-base) MAPSORT of: KIBB0034.seq Check: 2531 from: 1 to: 2109
KIBB0034.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 747 1616 1722 1809 2025 2109
Size: 747 869 106 87 216 84
Fragments arranged by size:
869 747 216 106 87 84
AccI GT'mk_AC
Cuts at: 0 1207 2109
Size: 1207 902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 637 661 1090 1321 2109
Size: 120 517 24 429 231 788
Fragments arranged by size:
788 517 429 231 120 24
AflIII A'CryG_T
Cuts at: 0 1475 2109
Size: 1475 634
AleI CACnn'nnGTG
Cuts at: 0 250 949 1150 2109
Size: 250 699 201 959
Fragments arranged by size:
959 699 250 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 383 885 1345 1597 2109
Size: 43 340 502 460 252 512
Fragments arranged by size:
512 502 460 340 252 43
AlwNI CAG_nnn'CTG
Cuts at: 0 579 1236 1434 2109
Size: 579 657 198 675
Fragments arranged by size:
675 657 579 198
ApaI G_GGCC'C
Cuts at: 0 490 610 1250 1899 2003 2109
Size: 490 120 640 649 104 106
Fragments arranged by size:
649 640 490 120 106 104
AscI GG'CGCG_CC
Cuts at: 0 1722 2109
Size: 1722 387
AvaI C'yCGr_G
Cuts at: 0 84 1933 2109
Size: 84 1849 176
Fragments arranged by size:
1849 176 84
BamHI G'GATC_C
Cuts at: 0 674 2109
Size: 674 1435
BanI G'GyrC_C
Cuts at: 0 57 541 1621 2109
Size: 57 484 1080 488
Fragments arranged by size:
1080 488 484 57
BanII G_rGCy'C
Cuts at: 0 272 490 610 629 1170 1250 1867 1899
Size: 272 218 120 19 541 80 617 32
Cuts at: 1899 2003 2109
Size: 104 106
Fragments arranged by size:
617 541 272 218 120 106 104 80
32 19
BbeI G_GCGC'C
Cuts at: 0 1625 2109
Size: 1625 484
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 954 2109
Size: 72 882 1155
Fragments arranged by size:
1155 882 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1432 1466 2068 2102 2109
Size: 1432 34 602 34 7
Fragments arranged by size:
1432 602 34 34 7
BglI GCCn_nnn'nGGC
Cuts at: 0 775 1936 2109
Size: 775 1161 173
Fragments arranged by size:
1161 775 173
BglII A'GATC_T
Cuts at: 0 680 2109
Size: 680 1429
Bme1580I G_kGCm'C
Cuts at: 0 22 149 318 334 490 610 1250 1899
Size: 22 127 169 16 156 120 640 649
Cuts at: 1899 2003 2109
Size: 104 106
Fragments arranged by size:
649 640 169 156 127 120 106 104
22 16
BmgBI CAC'GTC
Cuts at: 0 248 1148 2109
Size: 248 900 961
Fragments arranged by size:
961 900 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 1644 2109
Size: 1644 465
BmtI G_CTAG'C
Cuts at: 0 1784 2109
Size: 1784 325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1959 2109
Size: 1959 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 931 1309 1948 2048 2109
Size: 286 645 378 639 100 61
Fragments arranged by size:
645 639 378 286 100 61
Bpu10I CC'TnA_GC
Cuts at: 0 580 611 2109
Size: 580 31 1498
Fragments arranged by size:
1498 580 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1369 2109
Size: 310 1059 740
Fragments arranged by size:
1059 740 310
BsaI GGTCTCn'nnnn_
Cuts at: 0 1210 1743 2109
Size: 1210 533 366
Fragments arranged by size:
1210 533 366
BsaAI yAC'GTr
Cuts at: 0 1478 2109
Size: 1478 631
BsaBI GATnn'nnATC
Cuts at: 0 679 1369 2109
Size: 679 690 740
Fragments arranged by size:
740 690 679
BsaHI Gr'CG_yC
Cuts at: 0 689 1622 2109
Size: 689 933 487
Fragments arranged by size:
933 689 487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 727 757 1030 1060 1249 1279
Size: 541 30 156 30 273 30 189 30
Cuts at: 1279 2021 2051 2109
Size: 742 30 58
Fragments arranged by size:
742 541 273 189 156 58 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 748 1273 1498 1657 1771 1932 2109
Size: 613 135 525 225 159 114 161 177
Fragments arranged by size:
613 525 225 177 161 159 135 114
BseYI C'CCAG_C
Cuts at: 0 38 603 1023 1236 1675 1681 1903 1916
Size: 38 565 420 213 439 6 222 13
Cuts at: 1916 2011 2047 2109
Size: 95 36 62
Fragments arranged by size:
565 439 420 222 213 95 62 38
36 13 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 592 772 781 1210 1564 1588 2108
Size: 248 344 180 9 429 354 24 520
Cuts at: 2108 2109
Size: 1
Fragments arranged by size:
520 429 354 344 248 180 24 9
1
BsiHKAI G_wGCw'C
Cuts at: 0 170 272 629 824 2109
Size: 170 102 357 195 1285
Fragments arranged by size:
1285 357 195 170 102
BsiWI C'GTAC_G
Cuts at: 0 1478 2109
Size: 1478 631
Bsp1286I G_dGCh'C
Cuts at: 0 22 149 170 272 318 334 490 610
Size: 22 127 21 102 46 16 156 120
Cuts at: 610 629 824 1170 1250 1867 1899 2003 2109
Size: 19 195 346 80 617 32 104 106
Fragments arranged by size:
617 346 195 156 127 120 106 104
102 80 46 32 22 21 19 16
BspHI T'CATG_A
Cuts at: 0 1505 2109
Size: 1505 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 747 1411 1610 1616 1722 1809 2025
Size: 189 558 664 199 6 106 87 216
Cuts at: 2025 2109
Size: 84
Fragments arranged by size:
664 558 216 199 189 106 87 84
6
BsrBI CCG'CTC
Cuts at: 0 195 348 660 1293 2109
Size: 195 153 312 633 816
Fragments arranged by size:
816 633 312 195 153
BsrFI r'CCGG_y
Cuts at: 0 765 1876 2109
Size: 765 1111 233
Fragments arranged by size:
1111 765 233
BssHII G'CGCG_C
Cuts at: 0 724 1722 2109
Size: 724 998 387
Fragments arranged by size:
998 724 387
BssSI C'ACGA_G
Cuts at: 0 142 209 2109
Size: 142 67 1900
Fragments arranged by size:
1900 142 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 759 1974 2109
Size: 426 333 1215 135
Fragments arranged by size:
1215 426 333 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1734 2026 2109
Size: 1734 292 83
Fragments arranged by size:
1734 292 83
BstYI r'GATC_y
Cuts at: 0 575 674 680 836 1172 1826 2109
Size: 575 99 6 156 336 654 283
Fragments arranged by size:
654 575 336 283 156 99 6
BstZ17I GTA'TAC
Cuts at: 0 1208 2109
Size: 1208 901
Bsu36I CC'TnA_GG
Cuts at: 0 467 1922 2109
Size: 467 1455 187
Fragments arranged by size:
1455 467 187
BtgI C'CryG_G
Cuts at: 0 251 296 634 1538 2109
Size: 251 45 338 904 571
Fragments arranged by size:
904 571 338 251 45
BtsI GCAGTG_nn'
Cuts at: 0 318 447 676 2109
Size: 318 129 229 1433
Fragments arranged by size:
1433 318 229 129
DraIII CAC_nnn'GTG
Cuts at: 0 705 2109
Size: 705 1404
DrdI GACnn_nn'nnGTC
Cuts at: 0 1576 2109
Size: 1576 533
EaeI y'GGCC_r
Cuts at: 0 767 1613 2109
Size: 767 846 496
Fragments arranged by size:
846 767 496
EarI CTCTTCn'nnn_
Cuts at: 0 100 1330 2109
Size: 100 1230 779
Fragments arranged by size:
1230 779 100
EcoICRI GAG'CTC
Cuts at: 0 270 627 2109
Size: 270 357 1482
Fragments arranged by size:
1482 357 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 286 637 661 931 1090 1309 1321
Size: 120 166 351 24 270 159 219 12
Cuts at: 1321 1948 2048 2109
Size: 627 100 61
Fragments arranged by size:
627 351 270 219 166 159 120 100
61 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 1228 2109
Size: 856 372 881
Fragments arranged by size:
881 856 372
EcoO109I rG'GnC_Cy
Cuts at: 0 201 486 487 607 915 1246 1284 1895
Size: 201 285 1 120 308 331 38 611
Cuts at: 1895 1896 2000 2109
Size: 1 104 109
Fragments arranged by size:
611 331 308 285 201 120 109 104
38 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 1359 2109
Size: 744 615 750
Fragments arranged by size:
750 744 615
HaeII r_GCGC'y
Cuts at: 0 1357 1625 2109
Size: 1357 268 484
Fragments arranged by size:
1357 484 268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 727 759 833 865 1030 1062
Size: 203 32 492 32 74 32 165 32
Cuts at: 1062 1157 1189 1309 1341 2023 2055 2109
Size: 95 32 120 32 682 32 54
Fragments arranged by size:
682 492 203 165 120 95 74 54
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1621 2109
Size: 1621 488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1391 2109
Size: 1391 718
MslI CAynn'nnrTG
Cuts at: 0 250 365 511 949 982 1150 1465 1579
Size: 250 115 146 438 33 168 315 114
Cuts at: 1579 2109
Size: 530
Fragments arranged by size:
530 438 315 250 168 146 115 114
33
MspA1I CmG'CkG
Cuts at: 0 42 636 666 1281 1540 1658 1903 2109
Size: 42 594 30 615 259 118 245 206
Fragments arranged by size:
615 594 259 245 206 118 42 30
NaeI GCC'GGC
Cuts at: 0 767 1878 2109
Size: 767 1111 231
Fragments arranged by size:
1111 767 231
NarI GG'CG_CC
Cuts at: 0 1622 2109
Size: 1622 487
NgoMIV G'CCGG_C
Cuts at: 0 765 1876 2109
Size: 765 1111 233
Fragments arranged by size:
1111 765 233
NheI G'CTAG_C
Cuts at: 0 1780 2109
Size: 1780 329
NspI r_CATG'y
Cuts at: 0 516 1182 2109
Size: 516 666 927
Fragments arranged by size:
927 666 516
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 1236 1634 2109
Size: 264 972 398 475
Fragments arranged by size:
972 475 398 264
PfoI T'CCnGG_A
Cuts at: 0 908 1286 1346 1427 1748 2109
Size: 908 378 60 81 321 361
Fragments arranged by size:
908 378 361 321 81 60
PpuMI rG'GwC_Cy
Cuts at: 0 201 1284 2109
Size: 201 1083 825
Fragments arranged by size:
1083 825 201
PspOMI G'GGCC_C
Cuts at: 0 486 606 1246 1895 1999 2109
Size: 486 120 640 649 104 110
Fragments arranged by size:
649 640 486 120 110 104
PstI C_TGCA'G
Cuts at: 0 434 1124 1364 1499 1523 2109
Size: 434 690 240 135 24 586
Fragments arranged by size:
690 586 434 240 135 24
PvuII CAG'CTG
Cuts at: 0 42 2109
Size: 42 2067
SacI G_AGCT'C
Cuts at: 0 272 629 2109
Size: 272 357 1480
Fragments arranged by size:
1480 357 272
SacII CC_GC'GG
Cuts at: 0 637 1541 2109
Size: 637 904 568
Fragments arranged by size:
904 637 568
SbfI CC_TGCA'GG
Cuts at: 0 1523 2109
Size: 1523 586
SexAI A'CCwGG_T
Cuts at: 0 587 2109
Size: 587 1522
SfcI C'TryA_G
Cuts at: 0 430 1120 1360 1495 1519 2109
Size: 430 690 240 135 24 590
Fragments arranged by size:
690 590 430 240 135 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 775 2109
Size: 775 1334
SfoI GGC'GCC
Cuts at: 0 1623 2109
Size: 1623 486
SmlI C'TyrA_G
Cuts at: 0 325 1384 2109
Size: 325 1059 725
Fragments arranged by size:
1059 725 325
StuI AGG'CCT
Cuts at: 0 1835 1939 2109
Size: 1835 104 170
Fragments arranged by size:
1835 170 104
StyI C'CwwG_G
Cuts at: 0 476 527 719 746 815 857 1253 1845
Size: 476 51 192 27 69 42 396 592
Cuts at: 1845 1995 2109
Size: 150 114
Fragments arranged by size:
592 476 396 192 150 114 69 51
42 27
TatI w'GTAC_w
Cuts at: 0 1031 1646 2109
Size: 1031 615 463
Fragments arranged by size:
1031 615 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 541 573 1249 1281 1973 2005 2021 2053
Size: 541 32 676 32 692 32 16 32
Cuts at: 2053 2109
Size: 56
Fragments arranged by size:
692 676 541 56 32 32 32 32
16
Tth111I GACn'n_nGTC
Cuts at: 0 1816 2109
Size: 1816 293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1461 2109
Size: 1461 648
XmnI GAAnn'nnTTC
Cuts at: 0 94 1339 2109
Size: 94 1245 770
Fragments arranged by size:
1245 770 94
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
AscI AvaI BamHI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SbfI SexAI
SfcI SfiI SfoI SmlI StuI StyI TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BfrBI BlpI BsaWI BsiEI BsmI BsmBI BspEI
BsrDI BsrGI BstBI BstEII BtgZI ClaI CspCI DraI
EagI EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI ScaI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XhoI XmaI
ZraI