Restriction map for pF1KSDB0033
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccI        AclI        AflIII      AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
AspEI       AspI        AvrII       BamHI       BbeI        
BbsI        BcgI        BclI        BglI        BlnI        
BpuAI       BsmI        BspDI       BspHI       BspLU11I    
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        Eam1105I    Eco47III    EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KasI        KspI        MfeI        MluI        
MunI        NarI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I         21  GGTACC               G^GTACC
AflII        1708  CTTAAG               C^TTAAG
Alw44I        682  GTGCAC               G^TGCAC
AocI         2457  CCTGAGG              CC^T[ACGT]AGG
ApaI         3081  GGGCCC               GGGCC^C
ApaLI         682  GTGCAC               G^TGCAC
Asp718I        21  GGTACC               G^GTACC
BbvCI        1023  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1542  GGATAC               GTATCC(6/5)
BfaI         2630  CTAG                 C^TAG
BfrI         1708  CTTAAG               C^TTAAG
BlpI          912  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      912  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2142  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        1124  CGTACG               C^GTACG
BsrDI         974  GCAATG               GCAATG(2/0)
Bsu36I       2457  CCTGAGG              CC^T[ACGT]AGG
CelII         912  GCTGAGC              GC^T[ACGT]AGC
Eco81I       2457  CCTGAGG              CC^T[ACGT]AGG
HaeII           3  GGCGCT               [GA]GCGC^[CT]
KpnI           21  GGTACC               GGTAC^C
MaeI         2630  CTAG                 C^TAG
MamI         2142  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          104  CCATGG               C^CATGG
NdeI          298  CATATG               CA^TATG
PflMI         723  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          750  GACTC                GAGTC(4/5)
SmaI         1501  CCCGGG               CCC^GGG
Van91I        723  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1598  CCAGGAAATCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1501  CCCGGG               C^CCGGG
XmaI         1501  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        569  TCCGGA               T^CCGGA
             1700  TCCGGA              
Asp700I      1276  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2412  GAACGCCTTC          
BbrPI        2052  CACGTG               CAC^GTG
             2992  CACGTG              
BsaAI        2052  CACGTG               [CT]AC^GT[GA]
             2992  CACGTG              
BseAI         569  TCCGGA               T^CCGGA
             1700  TCCGGA              
Bsp1407I     1154  TGTACA               T^GTACA
             2940  TGTACA              
BspEI         569  TCCGGA               T^CCGGA
             1700  TCCGGA              
BsrBI         423  GAGCGG               CCGCTC(-3/-3)
              700  GAGCGG              
BsrGI        1154  TGTACA               T^GTACA
             2940  TGTACA              
BstAPI       2604  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2814  GCAGCTGCTGC         
DraIII       1930  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2490  CACCTCGTG           
DrdI          847  GACTGGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2854  GACGCCAAAGTC        
EagI         1902  CGGCCG               C^GGCCG
             2065  CGGCCG              
EclXI        1902  CGGCCG               C^GGCCG
             2065  CGGCCG              
Eco52I       1902  CGGCCG               C^GGCCG
             2065  CGGCCG              
EcoRI        1621  GAATTC               G^AATTC
             3180  GAATTC              
HindIII        70  AAGCTT               A^AGCTT
             2006  AAGCTT              
MroI          569  TCCGGA               T^CCGGA
             1700  TCCGGA              
MseI         1460  TTAA                 T^TAA
             1709  TTAA                
MslI           39  CATCCGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              603  CATGGATGTG          
NaeI          290  GCCGGC               GCC^GGC
             1978  GCCGGC              
NgoMIV        290  GCCGGC               G^CCGGC
             1978  GCCGGC              
PmaCI        2052  CACGTG               CAC^GTG
             2992  CACGTG              
PmlI         2052  CACGTG               CAC^GTG
             2992  CACGTG              
PpuMI         272  GGGACCT              [GA]G^G[AT]CC[CT]
              905  AGGACCT             
SapI         1341  GCTCTTC              GCTCTTC(1/4)
             2682  GCTCTTC             
SexAI         797  ACCTGGT              A^CC[AT]GGT
             1931  ACCAGGT             
SspBI        1154  TGTACA               T^GTACA
             2940  TGTACA              
Tru9I        1460  TTAA                 T^TAA
             1709  TTAA                
XmnI         1276  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2412  GAACGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbvI        BcnI        BglII       BmyI        
BpmI        BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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