Restriction map for
pF1KSDB0029
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BcnI BfaI BfrI
BlnI BsaI BsiWI BsmI Bsp1407I
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DraI DraII DraIII DrdI
Eam1105I Eco47III EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI HindIII KpnI
MaeI MluI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 1432 GTCTAC GT^[AC][GT]AC
AccIII 939 TCCGGA T^CCGGA
AcyI 1671 GGCGCC G[GA]^CG[CT]C
AocI 1054 CCTGAGG CC^T[ACGT]AGG
AspHI 538 GAGCTC G[AT]GC[AT]^C
AvaI 1556 CTCGGG C^[CT]CG[GA]G
BamHI 1236 GGATCC G^GATCC
BbeI 1671 GGCGCC GGCGC^C
BbrPI 1378 CACGTG CAC^GTG
BbsI 165 GAAGAC GAAGAC(2/6)
BbvCI 697 GCTGAGG CCTCAGC(-5/-2)
BcgI 1658 GCAAGCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1182 GTATCC GTATCC(6/5)
BlpI 1649 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1649 GCTCAGC GC^T[ACGT]AGC
BpuAI 165 GAAGAC GAAGAC(2/6)
BsaAI 1378 CACGTG [CT]AC^GT[GA]
BsaHI 1671 GGCGCC G[GA]^CG[CT]C
BsaWI 939 TCCGGA [AT]^CCGG[AT]
BseAI 939 TCCGGA T^CCGGA
BsiHKAI 538 GAGCTC G[AT]GC[AT]^C
BsoBI 1556 CTCGGG C^[CT]CG[GA]G
BspDI 208 ATCGAT AT^CGAT
BspEI 939 TCCGGA T^CCGGA
BspHI 764 TCATGA T^CATGA
BsrBI 1078 GAGCGG CCGCTC(-3/-3)
BssSI 36 CACGAG CACGAG(-5/-1)
Bsu36I 1054 CCTGAGG CC^T[ACGT]AGG
CelII 1649 GCTCAGC GC^T[ACGT]AGC
ClaI 208 ATCGAT AT^CGAT
DsaI 28 CCGCGG C^C[GA][CT]GG
Eco81I 1054 CCTGAGG CC^T[ACGT]AGG
EcoRV 607 GATATC GAT^ATC
HaeII 1671 GGCGCC [GA]GCGC^[CT]
HapII 940 CCGG C^CGG
HgaI 440 GCGTC GACGC(5/10)
Hin1I 1671 GGCGCC G[GA]^CG[CT]C
HincII 481 GTTAAC GT[CT]^[GA]AC
HindII 481 GTTAAC GT[CT]^[GA]AC
HpaI 481 GTTAAC GTT^AAC
HpaII 940 CCGG C^CGG
KasI 1671 GGCGCC G^GCGCC
KspI 28 CCGCGG CCGC^GG
MroI 939 TCCGGA T^CCGGA
MspI 940 CCGG C^CGG
NarI 1671 GGCGCC GG^CGCC
PflMI 1134 CCAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GAGTC GAGTC(4/5)
PmaCI 1378 CACGTG CAC^GTG
PmlI 1378 CACGTG CAC^GTG
PvuII 779 CAGCTG CAG^CTG
RcaI 764 TCATGA T^CATGA
SacI 538 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SapI 685 GAAGAGC GCTCTTC(1/4)
SfiI 655 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1671 GGCGCC GGC^GCC
SmlI 584 CTCAAG C^T[CT][GA]AG
SphI 1473 GCATGC GCATG^C
StuI 353 AGGCCT AGG^CCT
TaqI 209 TCGA T^CGA
TthHB8I 209 TCGA T^CGA
Van91I 1134 CCAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 29 CGCG CG^CG
1318 CGCG
AlwNI 1241 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1547 CAGGCTCTG
BalI 962 TGGCCA TGG^CCA
1109 TGGCCA
BclI 81 TGATCA T^GATCA
308 TGATCA
BglII 548 AGATCT A^GATCT
1169 AGATCT
BpmI 155 CTGGAG CTGGAG(16/14)
200 CTGGAG
BsaBI 189 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
514 GATTTGTATC
BsiEI 1573 CGGCCG CG[GA][CT]^CG
1683 CGGCCG
BsmBI 46 CGTCTC CGTCTC(1/5)
1257 CGTCTC
BstAPI 447 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1546 GCAGGCTCTGC
BstUI 29 CGCG CG^CG
1318 CGCG
BstXI 1064 CCAATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGGGGGCTGG
EagI 1573 CGGCCG C^GGCCG
1683 CGGCCG
EarI 387 GAAGAG CTCTTC(1/4)
685 GAAGAG
EclXI 1573 CGGCCG C^GGCCG
1683 CGGCCG
Eco52I 1573 CGGCCG C^GGCCG
1683 CGGCCG
FbaI 81 TGATCA T^GATCA
308 TGATCA
Ksp632I 387 GAAGAG CTCTTC(1/4)
685 GAAGAG
MaeII 752 ACGT A^CGT
1379 ACGT
MamI 189 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
514 GATTTGTATC
MfeI 738 CAATTG C^AATTG
1025 CAATTG
MluNI 962 TGGCCA TGG^CCA
1109 TGGCCA
MscI 962 TGGCCA TGG^CCA
1109 TGGCCA
MunI 738 CAATTG C^AATTG
1025 CAATTG
MvnI 29 CGCG CG^CG
1318 CGCG
PstI 976 CTGCAG CTGCA^G
1410 CTGCAG
SfcI 976 CTGCAG C^T[GA][CT]AG
1410 CTGCAG
TfiI 68 GAATC G^A[AT]TC
1593 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AspI
BanI BanII BbvI BglI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflFI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI XhoII