Restriction map for pF1KSDB0028
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BlnI        
BsaAI       BsaBI       BsaHI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AclI         1239  AACGTT               AA^CGTT
AcsI         1401  GAATTT               [GA]^AATT[CT]
AflIII        752  ACATGT               A^C[GA][CT]GT
AocI          890  CCTCAGG              CC^T[ACGT]AGG
ApaI          305  GGGCCC               GGGCC^C
ApoI         1401  GAATTT               [GA]^AATT[CT]
AspHI        1467  GAGCTC               G[AT]GC[AT]^C
AvaI          164  CTCGGG               C^[CT]CG[GA]G
BanI          970  GGTGCC               G^G[CT][GA]CC
BbsI          297  GAAGAC               GAAGAC(2/6)
BciVI        1362  GGATAC               GTATCC(6/5)
BfaI         1460  CTAG                 C^TAG
BglI          430  GCCCAGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         465  AGATCT               A^GATCT
BlpI         1006  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1006  GCTCAGC              GC^T[ACGT]AGC
BpuAI         297  GAAGAC               GAAGAC(2/6)
BsaI         1033  GGTCTC               GGTCTC(1/5)
BsaWI         119  TCCGGT               [AT]^CCGG[AT]
BsgI          845  GTGCAG               GTGCAG(16/14)
BsiHKAI      1467  GAGCTC               G[AT]GC[AT]^C
BsoBI         164  CTCGGG               C^[CT]CG[GA]G
BspHI         330  TCATGA               T^CATGA
BspLU11I      752  ACATGT               A^CATGT
BsrFI        1657  GCCGGT               [GA]^CCGG[CT]
BstAPI       1489  GCATCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        890  CCTCAGG              CC^T[ACGT]AGG
CelII        1006  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1657  GCCGGT               [GA]^CCGG[CT]
DraI          103  TTTAAA               TTT^AAA
Eco81I        890  CCTCAGG              CC^T[ACGT]AGG
EcoT14I      1140  CCAAGG               C^C[AT][AT]GG
FauI         1283  CCCGC                CCCGC(4/6)
MaeI         1460  CTAG                 C^TAG
MspA1I       1127  CAGCTG               C[AC]G^C[GT]G
NspI          752  ACATGT               [GA]CATG^[CT]
PflMI        1013  CCATCGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1143  AGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1239  AACGTT               AA^CGTT
PvuII        1127  CAGCTG               CAG^CTG
RcaI          330  TCATGA               T^CATGA
SacI         1467  GAGCTC               GAGCT^C
SapI         1558  GAAGAGC              GCTCTTC(1/4)
SexAI        1189  ACCAGGT              A^CC[AT]GGT
SspI          561  AATATT               AAT^ATT
StyI         1140  CCAAGG               C^C[AT][AT]GG
TaqI         1432  TCGA                 T^CGA
TthHB8I      1432  TCGA                 T^CGA
Van91I       1013  CCATCGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1217  CCAGGCAGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII         117  GGTCC                G^G[AT]CC
             1144  GGTCC               
BalI          416  TGGCCA               TGG^CCA
             1060  TGGCCA              
BsrDI         345  CATTGC               GCAATG(2/0)
              904  GCAATG              
BssSI         619  CACGAG               CACGAG(-5/-1)
             1176  CACGAG              
BstYI         465  AGATCT               [GA]^GATC[CT]
              582  AGATCC              
HincII          3  GTCAAC               GT[CT]^[GA]AC
              710  GTCAAC              
HindII          3  GTCAAC               GT[CT]^[GA]AC
              710  GTCAAC              
MaeII         899  ACGT                 A^CGT
             1240  ACGT                
MflI          465  AGATCT               [GA]^GATC[CT]
              582  AGATCC              
MluNI         416  TGGCCA               TGG^CCA
             1060  TGGCCA              
MscI          416  TGGCCA               TGG^CCA
             1060  TGGCCA              
MslI          316  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1495  CATGCCAGTG          
PleI           52  GACTC                GAGTC(4/5)
             1183  GACTC               
SmlI          202  CTCAAG               C^T[CT][GA]AG
              459  CTTGAG              
StuI         1322  AGGCCT               AGG^CCT
             1586  AGGCCT              
XhoII         465  AGATCT               [GA]^GATC[CT]
              582  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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