Restriction map for pF1KSDB0028
 (Linear) (Six-base) MAPSORT of: KIBB0028.seq  Check: 7962  from: 1  to: 1665



KIBB0028.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AclI AA'CG_TT

Cuts at:      0    1240    1665 
   Size:       1240     425
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1665 
   Size:        295    1370
 
AflIII A'CryG_T

Cuts at:      0     752    1665 
   Size:        752     913
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     338    1665 
   Size:        338    1327
 
ApaI G_GGCC'C

Cuts at:      0     309    1665 
   Size:        309    1356
 
ApoI r'AATT_y

Cuts at:      0    1401    1665 
   Size:       1401     264
 
AvaI C'yCGr_G

Cuts at:      0     164    1665 
   Size:        164    1501
 
BanI G'GyrC_C

Cuts at:      0     970    1665 
   Size:        970     695
 
BanII G_rGCy'C

Cuts at:      0     309    1008    1471    1619    1631    1665 
   Size:        309     699     463     148      12      34

  Fragments arranged by size: 

                 699     463     309     148      34      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     304    1665 
   Size:        304    1361
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1356    1665 
   Size:       1356     309
 
BglI GCCn_nnn'nGGC

Cuts at:      0     436    1665 
   Size:        436    1229
 
BglII A'GATC_T

Cuts at:      0     465    1665 
   Size:        465    1200
 
BlpI GC'TnA_GC

Cuts at:      0    1007    1665 
   Size:       1007     658
 
Bme1580I G_kGCm'C

Cuts at:      0     309     975    1280    1665 
   Size:        309     666     305     385

  Fragments arranged by size: 

                 666     385     309     305
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1389    1613    1625    1665 
   Size:       1389     224      12      40

  Fragments arranged by size: 

                1389     224      40      12
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1344    1665 
   Size:       1344     321
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     782    1314    1665 
   Size:         39     743     532     351

  Fragments arranged by size: 

                 743     532     351      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     989    1665 
   Size:        989     676
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     480    1665 
   Size:        187     293    1185

  Fragments arranged by size: 

                1185     293     187
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1039    1665 
   Size:       1039     626
 
BsaWI w'CCGG_w

Cuts at:      0     119    1665 
   Size:        119    1546
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1285    1315    1665 
   Size:       1285      30     350

  Fragments arranged by size: 

                1285     350      30
 
BseYI C'CCAG_C

Cuts at:      0     974    1262    1452    1546    1665 
   Size:        974     288     190      94     119

  Fragments arranged by size: 

                 974     288     190     119      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866    1665 
   Size:        866     799
 
BsiHKAI G_wGCw'C

Cuts at:      0    1471    1665 
   Size:       1471     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     309     975    1008    1280    1471    1619    1631    1665 
   Size:        309     666      33     272     191     148      12      34

  Fragments arranged by size: 

                 666     309     272     191     148      34      33      12
            
 
BspHI T'CATG_A

Cuts at:      0     330    1665 
   Size:        330    1335
 
BsrDI GCAATG_nn'

Cuts at:      0     344     911    1665 
   Size:        344     567     754

  Fragments arranged by size: 

                 754     567     344
 
BsrFI r'CCGG_y

Cuts at:      0    1657    1665 
   Size:       1657       8
 
BssSI C'ACGA_G

Cuts at:      0     619    1176    1665 
   Size:        619     557     489

  Fragments arranged by size: 

                 619     557     489
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1495    1665 
   Size:       1495     170
 
BstYI r'GATC_y

Cuts at:      0     465     582    1665 
   Size:        465     117    1083

  Fragments arranged by size: 

                1083     465     117
 
Bsu36I CC'TnA_GG

Cuts at:      0     891    1665 
   Size:        891     774
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     999    1665 
   Size:        999     666
 
BtsI GCAGTG_nn'

Cuts at:      0      84     541     875    1665 
   Size:         84     457     334     790

  Fragments arranged by size: 

                 790     457     334      84
 
DraI TTT'AAA

Cuts at:      0     105    1665 
   Size:        105    1560
 
EaeI y'GGCC_r

Cuts at:      0     416    1060    1070    1665 
   Size:        416     644      10     595

  Fragments arranged by size: 

                 644     595     416      10
 
EarI CTCTTCn'nnn_

Cuts at:      0     563    1523    1553    1665 
   Size:        563     960      30     112

  Fragments arranged by size: 

                 960     563     112      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1073    1259    1665 
   Size:       1073     186     406

  Fragments arranged by size: 

                1073     406     186
 
EcoICRI GAG'CTC

Cuts at:      0    1469    1665 
   Size:       1469     196
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     295     782    1314    1665 
   Size:         39     256     487     532     351

  Fragments arranged by size: 

                 532     487     351     256      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     305     306    1144    1665 
   Size:        305       1     838     521

  Fragments arranged by size: 

                 838     521     305       1
 
HaeII r_GCGC'y

Cuts at:      0      44     224     914    1665 
   Size:         44     180     690     751

  Fragments arranged by size: 

                 751     690     180      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     760     792    1285    1317    1665 
   Size:        760      32     493      32     348

  Fragments arranged by size: 

                 760     493     348      32      32
 
HincII GTy'rAC

Cuts at:      0       5     712    1665 
   Size:          5     707     953

  Fragments arranged by size: 

                 953     707       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     975    1471    1619    1665 
   Size:        975     496     148      46

  Fragments arranged by size: 

                 975     496     148      46
 
MscI TGG'CCA

Cuts at:      0     418    1062    1665 
   Size:        418     644     603

  Fragments arranged by size: 

                 644     603     418
 
MslI CAynn'nnrTG

Cuts at:      0     320    1499    1665 
   Size:        320    1179     166

  Fragments arranged by size: 

                1179     320     166
 
MspA1I CmG'CkG

Cuts at:      0    1129    1665 
   Size:       1129     536
 
NspI r_CATG'y

Cuts at:      0     756    1665 
   Size:        756     909
 
PciI A'CATG_T

Cuts at:      0     752    1665 
   Size:        752     913
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1019    1665 
   Size:       1019     646
 
PpuMI rG'GwC_Cy

Cuts at:      0    1144    1665 
   Size:       1144     521
 
PspOMI G'GGCC_C

Cuts at:      0     305    1665 
   Size:        305    1360
 
PvuII CAG'CTG

Cuts at:      0    1129    1665 
   Size:       1129     536
 
SacI G_AGCT'C

Cuts at:      0    1471    1665 
   Size:       1471     194
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1553    1665 
   Size:       1553     112
 
SexAI A'CCwGG_T

Cuts at:      0    1189    1665 
   Size:       1189     476
 
SmlI C'TyrA_G

Cuts at:      0     202     459    1665 
   Size:        202     257    1206

  Fragments arranged by size: 

                1206     257     202
 
SspI AAT'ATT

Cuts at:      0     563    1665 
   Size:        563    1102
 
StuI AGG'CCT

Cuts at:      0    1324    1588    1665 
   Size:       1324     264      77

  Fragments arranged by size: 

                1324     264      77
 
StyI C'CwwG_G

Cuts at:      0    1140    1665 
   Size:       1140     525
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     958    1222    1665 
   Size:        958     264     443

  Fragments arranged by size: 

                 958     443     264
 
TatI w'GTAC_w

Cuts at:      0     596    1665 
   Size:        596    1069
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     520     552    1665 
   Size:        520      32    1113

  Fragments arranged by size: 

                1113     520      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1224    1665 
   Size:       1224     441
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AlfI     ApaI     ApoI     AvaI     BanI
    BanII     BbsI    BciVI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrFI    BssSI   BstAPI
    BstYI   Bsu36I    BtgZI     BtsI     DraI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NspI     PciI    PflMI    PpuMI   PspOMI    PvuII
     SacI     SapI    SexAI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI    BmgBI
     BmtI    BsaAI    BsaBI    BsaHI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BstBI   BstEII
    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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