Restriction map for pF1KB8806
 (Linear) (Six-base) MAPSORT of: KIBB8806.seq  Check: 2794  from: 1  to: 1065



KIBB8806.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     553    1065 
   Size:        553     512
 
Acc65I G'GTAC_C

Cuts at:      0     392    1065 
   Size:        392     673
 
AleI CACnn'nnGTG

Cuts at:      0     881     953    1065 
   Size:        881      72     112

  Fragments arranged by size: 

                 881     112      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84    1065 
   Size:         84     981
 
AlwNI CAG_nnn'CTG

Cuts at:      0     600    1065 
   Size:        600     465
 
ApaI G_GGCC'C

Cuts at:      0     102     477    1065 
   Size:        102     375     588

  Fragments arranged by size: 

                 588     375     102
 
ApaLI G'TGCA_C

Cuts at:      0     964    1065 
   Size:        964     101
 
ApoI r'AATT_y

Cuts at:      0     529    1065 
   Size:        529     536
 
BanI G'GyrC_C

Cuts at:      0     392    1065 
   Size:        392     673
 
BanII G_rGCy'C

Cuts at:      0     102     477    1065 
   Size:        102     375     588

  Fragments arranged by size: 

                 588     375     102
 
BclI T'GATC_A

Cuts at:      0     644     734    1065 
   Size:        644      90     331

  Fragments arranged by size: 

                 644     331      90
 
BglI GCCn_nnn'nGGC

Cuts at:      0      97     427     487    1065 
   Size:         97     330      60     578

  Fragments arranged by size: 

                 578     330      97      60
 
BglII A'GATC_T

Cuts at:      0     359    1065 
   Size:        359     706
 
Bme1580I G_kGCm'C

Cuts at:      0     102     432     477     968    1065 
   Size:        102     330      45     491      97

  Fragments arranged by size: 

                 491     330     102      97      45
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     303     886    1065 
   Size:        303     583     179

  Fragments arranged by size: 

                 583     303     179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1065 
   Size:       1027      38
 
BsaWI w'CCGG_w

Cuts at:      0     923    1065 
   Size:        923     142
 
BseYI C'CCAG_C

Cuts at:      0    1057    1065 
   Size:       1057       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     968    1065 
   Size:        968      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     102     432     477     968    1065 
   Size:        102     330      45     491      97

  Fragments arranged by size: 

                 491     330     102      97      45
 
BspEI T'CCGG_A

Cuts at:      0     923    1065 
   Size:        923     142
 
BspHI T'CATG_A

Cuts at:      0     641     731    1065 
   Size:        641      90     334

  Fragments arranged by size: 

                 641     334      90
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     248     553     941    1065 
   Size:        248     305     388     124

  Fragments arranged by size: 

                 388     305     248     124
 
BsrGI T'GTAC_A

Cuts at:      0     350    1065 
   Size:        350     715
 
BstYI r'GATC_y

Cuts at:      0     320     359    1065 
   Size:        320      39     706

  Fragments arranged by size: 

                 706     320      39
 
BtgI C'CryG_G

Cuts at:      0     995    1065 
   Size:        995      70
 
BtsI GCAGTG_nn'

Cuts at:      0     876    1065 
   Size:        876     189
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      27      62    1065 
   Size:         27      35    1003

  Fragments arranged by size: 

                1003      35      27
 
DraIII CAC_nnn'GTG

Cuts at:      0     414    1065 
   Size:        414     651
 
EaeI y'GGCC_r

Cuts at:      0     227     398     479    1065 
   Size:        227     171      81     586

  Fragments arranged by size: 

                 586     227     171      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     250    1065 
   Size:        250     815
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1065 
   Size:       1027      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89      98     474    1065 
   Size:         89       9     376     591

  Fragments arranged by size: 

                 591     376      89       9
 
EcoRI G'AATT_C

Cuts at:      0     529    1065 
   Size:        529     536
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     685     717    1065 
   Size:        685      32     348

  Fragments arranged by size: 

                 685     348      32
 
HincII GTy'rAC

Cuts at:      0     888    1065 
   Size:        888     177
 
KpnI G_GTAC'C

Cuts at:      0     396    1065 
   Size:        396     669
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     129    1065 
   Size:        129     936
 
MscI TGG'CCA

Cuts at:      0     229     400     481    1065 
   Size:        229     171      81     584

  Fragments arranged by size: 

                 584     229     171      81
 
MslI CAynn'nnrTG

Cuts at:      0     160     815     881     953    1065 
   Size:        160     655      66      72     112

  Fragments arranged by size: 

                 655     160     112      72      66
 
PsiI TTA'TAA

Cuts at:      0     667    1065 
   Size:        667     398
 
PspOMI G'GGCC_C

Cuts at:      0      98     473    1065 
   Size:         98     375     592

  Fragments arranged by size: 

                 592     375      98
 
PstI C_TGCA'G

Cuts at:      0     550    1065 
   Size:        550     515
 
RsrII CG'GwC_CG

Cuts at:      0     434    1065 
   Size:        434     631
 
SapI GCTCTTCn'nnn_

Cuts at:      0     250    1065 
   Size:        250     815
 
SexAI A'CCwGG_T

Cuts at:      0     968    1065 
   Size:        968      97
 
SfcI C'TryA_G

Cuts at:      0     546    1065 
   Size:        546     519
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      97    1065 
   Size:         97     968
 
StyI C'CwwG_G

Cuts at:      0     125    1065 
   Size:        125     940
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     823    1065 
   Size:        823     242
 
TatI w'GTAC_w

Cuts at:      0     119     350    1065 
   Size:        119     231     715

  Fragments arranged by size: 

                 715     231     119
 

 Enzymes that do cut:

     AarI   Acc65I     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     BanI    BanII     BclI     BglI    BglII Bme1580I     BmrI     BpmI
    BsaWI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrGI
    BstYI     BtgI     BtsI    CspCI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII     KpnI     MmeI     MscI     MslI
     PsiI   PspOMI     PstI    RsrII     SapI    SexAI     SfcI     SfiI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    PvuII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com