Restriction map for pF1KSDB0021
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
BamHI       BbrPI       BbvCI       BciVI       BclI        
BfrI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        DraI        DraIII      DrdI        Eam1105I    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        HincII      
HindII      HindIII     HpaI        MamI        MfeI        
MluI        MseI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        469  GGTACC               G^GTACC
AccI          136  GTCTAC               GT^[AC][GT]AC
AccIII        894  TCCGGA               T^CCGGA
Asp718I       469  GGTACC               G^GTACC
AspHI         960  GAGCAC               G[AT]GC[AT]^C
AviII         119  TGCGCA               TGC^GCA
AvrII         537  CCTAGG               C^CTAGG
BalI          356  TGGCCA               TGG^CCA
BbsI          490  GTCTTC               GAAGAC(2/6)
BcgI          962  GCACAGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          538  CTAG                 C^TAG
BlnI          537  CCTAGG               C^CTAGG
BpuAI         490  GTCTTC               GAAGAC(2/6)
BsaWI         894  TCCGGA               [AT]^CCGG[AT]
BseAI         894  TCCGGA               T^CCGGA
BsiHKAI       960  GAGCAC               G[AT]GC[AT]^C
BsmBI         318  CGTCTC               CGTCTC(1/5)
BspEI         894  TCCGGA               T^CCGGA
CpoI          891  CGGTCCG              CG^G[AT]CCG
EarI          871  GAAGAG               CTCTTC(1/4)
EcoT14I       537  CCTAGG               C^C[AT][AT]GG
FspI          119  TGCGCA               TGC^GCA
HphI          363  GGTGA                GGTGA(8/7)
KpnI          469  GGTACC               GGTAC^C
Ksp632I       871  GAAGAG               CTCTTC(1/4)
MaeI          538  CTAG                 C^TAG
MaeIII        364  GTGAC                ^GT[ACGT]AC
MluNI         356  TGGCCA               TGG^CCA
MroI          894  TCCGGA               T^CCGGA
MscI          356  TGGCCA               TGG^CCA
NlaIII        948  CATG                 CATG^
PshAI         366  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         891  CGGTCCG              CG^G[AT]CCG
SmlI          715  CTCAAG               C^T[CT][GA]AG
StyI          537  CCTAGG               C^C[AT][AT]GG
TaqI          706  TCGA                 T^CGA
Tsp45I        364  GTGAC                ^GT[GC]AC
TthHB8I       706  TCGA                 T^CGA
XcmI          235  CCACCCGCCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          470  GTAC                 GT^AC
              560  GTAC                
Aor51HI       308  AGCGCT               AGC^GCT
              653  AGCGCT              
BanII         619  GAGCCC               G[GA]GC[CT]^C
              907  GAGCCC              
BbeI          192  GGCGCC               GGCGC^C
              696  GGCGCC              
BglII          50  AGATCT               A^GATCT
              917  AGATCT              
BsiEI         227  CGGCCG               CG[GA][CT]^CG
              566  CGGCCG              
BsrI          367  ACTGG                ACTGG(1/-1)
              824  CCAGT               
BssHII        514  GCGCGC               G^CGCGC
              596  GCGCGC              
BssSI         272  CTCGTG               CACGAG(-5/-1)
              506  CTCGTG              
BstYI          50  AGATCT               [GA]^GATC[CT]
              917  AGATCT              
DpnII          51  GATC                 ^GATC
              918  GATC                
DraII         184  GGGACCC              [GA]G^G[ACGT]CC[CT]
              548  AGGACCC             
EagI          227  CGGCCG               C^GGCCG
              566  CGGCCG              
EclXI         227  CGGCCG               C^GGCCG
              566  CGGCCG              
Eco47III      308  AGCGCT               AGC^GCT
              653  AGCGCT              
Eco52I        227  CGGCCG               C^GGCCG
              566  CGGCCG              
EcoO109I      184  GGGACCC              [GA]G^G[ACGT]CC[CT]
              548  AGGACCC             
HinfI         371  GAGTC                G^A[ACGT]TC
              836  GACTC               
KasI          192  GGCGCC               G^GCGCC
              696  GGCGCC              
KspI          331  CCGCGG               CCGC^GG
              635  CCGCGG              
MaeII         380  ACGT                 A^CGT
              794  ACGT                
MboI           51  GATC                 ^GATC
              918  GATC                
MflI           50  AGATCT               [GA]^GATC[CT]
              917  AGATCT              
NaeI          222  GCCGGC               GCC^GGC
              241  GCCGGC              
NarI          192  GGCGCC               GG^CGCC
              696  GGCGCC              
NdeII          51  GATC                 ^GATC
              918  GATC                
NgoMIV        222  GCCGGC               G^CCGGC
              241  GCCGGC              
PleI          836  GACTC                GAGTC(4/5)
              371  GAGTC               
PpuMI         184  GGGACCC              [GA]G^G[AT]CC[CT]
              548  AGGACCC             
RsaI          470  GTAC                 GT^AC
              560  GTAC                
SacII         331  CCGCGG               CCGC^GG
              635  CCGCGG              
Sau3AI         51  GATC                 ^GATC
              918  GATC                
SfoI          192  GGCGCC               GGC^GCC
              696  GGCGCC              
XhoII          50  AGATCT               [GA]^GATC[CT]
              917  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
AvaII       BanI        BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DsaI        EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
ItaI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       SfaNI       SmaI        TseI        
XmaCI       XmaI        
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