Restriction map for
pF1KSDB0021
(Circular) (Six-base) MAPSORT of: pF1KSDB0021.seq Check: 8660 from: 1 to: 4120
pF1KSDB0021 4120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 263 1156 263
Size: 893 3227
Acc65I G'GTAC_C
Cuts at: 595 1134 595
Size: 539 3581
AclI AA'CG_TT
Cuts at: 3960 3960
Size: 4120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1943 2375 3124 3392 1943
Size: 432 749 268 2671
Fragments arranged by size:
2671 749 432 268
AfeI AGC'GCT
Cuts at: 436 781 436
Size: 345 3775
AflIII A'CryG_T
Cuts at: 1470 2576 3553 1470
Size: 1106 977 2037
Fragments arranged by size:
2037 1106 977
AgeI A'CCGG_T
Cuts at: 2508 2508
Size: 4120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4120
AlwNI CAG_nnn'CTG
Cuts at: 1423 2992 1423
Size: 1569 2551
ApaLI G'TGCA_C
Cuts at: 2890 2890
Size: 4120
ApoI r'AATT_y
Cuts at: 1122 3473 3729 1122
Size: 2351 256 1513
Fragments arranged by size:
2351 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4120
AvaI C'yCGr_G
Cuts at: 340 622 957 979 1036 1138 340
Size: 282 335 22 57 102 3322
Fragments arranged by size:
3322 335 282 102 57 22
AvrII C'CTAG_G
Cuts at: 663 663
Size: 4120
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4120
BanI G'GyrC_C
Cuts at: 318 474 595 822 1134 1548 1796 1831 318
Size: 156 121 227 312 414 248 35 2607
Fragments arranged by size:
2607 414 312 248 227 156 121 35
BanII G_rGCy'C
Cuts at: 749 1037 1132 2162 749
Size: 288 95 1030 2707
Fragments arranged by size:
2707 1030 288 95
BbeI G_GCGC'C
Cuts at: 322 826 1552 1800 322
Size: 504 726 248 2642
Fragments arranged by size:
2642 726 504 248
BbsI GAAGACnn'nnnn_
Cuts at: 609 609
Size: 4120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1077 1111 1077
Size: 34 4086
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2779 4120 2016
Size: 763 1341 2016
Fragments arranged by size:
2016 1341 763
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4120
BglI GCCn_nnn'nGGC
Cuts at: 653 665 812 983 1099 653
Size: 12 147 171 116 3674
Fragments arranged by size:
3674 171 147 116 12
BglII A'GATC_T
Cuts at: 176 1043 1633 3672 176
Size: 867 590 2039 624
Fragments arranged by size:
2039 867 624 590
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4120
Bme1580I G_kGCm'C
Cuts at: 283 772 1743 1836 2894 283
Size: 489 971 93 1058 1509
Fragments arranged by size:
1509 1058 971 489 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3892 1330
Size: 81 335 2146 1558
Fragments arranged by size:
2146 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 2485 456
Size: 2029 2091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 515 567 1104 160
Size: 355 52 537 3176
Fragments arranged by size:
3176 537 355 52
Bpu10I CC'TnA_GC
Cuts at: 268 268
Size: 4120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1294 2173 2667 2965 826
Size: 468 879 494 298 1981
Fragments arranged by size:
1981 879 494 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 237 409 904 3824 237
Size: 172 495 2920 533
Fragments arranged by size:
2920 533 495 172
BsaAI yAC'GTr
Cuts at: 1110 1471 1110
Size: 361 3759
BsaBI GATnn'nnATC
Cuts at: 6 1632 3671 6
Size: 1626 2039 455
Fragments arranged by size:
2039 1626 455
BsaHI Gr'CG_yC
Cuts at: 319 614 823 1549 1797 2501 3997 319
Size: 295 209 726 248 704 1496 442
Fragments arranged by size:
1496 726 704 442 295 248 209
BsaWI w'CCGG_w
Cuts at: 1020 1307 1828 2508 2782 2929 3340 1020
Size: 287 521 680 274 147 411 1800
Fragments arranged by size:
1800 680 521 411 287 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 429 896 916 1015 1024 429
Size: 467 20 99 9 3525
Fragments arranged by size:
3525 467 99 20 9
BseYI C'CCAG_C
Cuts at: 307 371 608 807 1543 2301 2880 3376 307
Size: 64 237 199 736 758 579 496 1051
Fragments arranged by size:
1051 758 736 579 496 237 199 64
BsiEI CG_ry'CG
Cuts at: 104 356 695 1660 1706 2916 104
Size: 252 339 965 46 1210 1308
Fragments arranged by size:
1308 1210 965 339 252 46
BsiHKAI G_wGCw'C
Cuts at: 1090 1132 1910 2100 2894 1090
Size: 42 778 190 794 2316
Fragments arranged by size:
2316 794 778 190 42
BsmBI CGTCTCn'nnnn_
Cuts at: 450 450
Size: 4120
Bsp1286I G_dGCh'C
Cuts at: 283 749 772 1037 1090 1132 1743 1836 1910
Size: 466 23 265 53 42 611 93 74
Cuts at: 1910 2100 2162 2894 283
Size: 190 62 732 1509
Fragments arranged by size:
1509 732 611 466 265 190 93 74
62 53 42 23
BspEI T'CCGG_A
Cuts at: 1020 3340 1020
Size: 2320 1800
BspHI T'CATG_A
Cuts at: 3296 3296
Size: 4120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1382 1684 2065 1168
Size: 214 302 381 3223
Fragments arranged by size:
3223 381 302 214
BsrBI CCG'CTC
Cuts at: 50 397 840 1016 2410 2464 3950 4115 50
Size: 347 443 176 1394 54 1486 165 55
Fragments arranged by size:
1486 1394 443 347 176 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4120
BsrFI r'CCGG_y
Cuts at: 298 348 367 477 690 1059 2116 2297 2508
Size: 50 19 110 213 369 1057 181 211
Cuts at: 2508 298
Size: 1910
Fragments arranged by size:
1910 1057 369 213 211 181 110 50
19
BssHII G'CGCG_C
Cuts at: 640 722 640
Size: 82 4038
BssSI C'ACGA_G
Cuts at: 398 632 2389 2749 398
Size: 234 1757 360 1769
Fragments arranged by size:
1769 1757 360 234
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4120
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4120
BstYI r'GATC_y
Cuts at: 1 176 1043 1143 1610 1633 1968 2214 3228
Size: 175 867 100 467 23 335 246 1014
Cuts at: 3228 3314 3326 3672 1
Size: 86 12 346 449
Fragments arranged by size:
1014 867 467 449 346 335 246 175
100 86 23 12
BtgI C'CryG_G
Cuts at: 125 457 485 761 125
Size: 332 28 276 3484
Fragments arranged by size:
3484 332 276 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 133 2068 2249 2412 133
Size: 1935 181 163 1841
Fragments arranged by size:
1935 1841 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4120
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4120
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2684 1824
Size: 860 3260
EaeI y'GGCC_r
Cuts at: 296 353 409 482 578 692 1703 1877 2268
Size: 57 56 73 96 114 1011 174 391
Cuts at: 2268 2295 3642 296
Size: 27 1347 774
Fragments arranged by size:
1347 1011 774 391 174 114 96 73
57 56 27
EagI C'GGCC_G
Cuts at: 353 692 1703 353
Size: 339 1011 2770
Fragments arranged by size:
2770 1011 339
EarI CTCTTCn'nnn_
Cuts at: 992 2141 2351 992
Size: 1149 210 2761
Fragments arranged by size:
2761 1149 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 533 541 2326 2638 2784 3930 533
Size: 8 1785 312 146 1146 723
Fragments arranged by size:
1785 1146 723 312 146 8
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 515 567 1104 1943 2375 3124 3392 160
Size: 355 52 537 839 432 749 268 888
Fragments arranged by size:
888 839 749 537 432 355 268 52
EcoO109I rG'GnC_Cy
Cuts at: 311 675 1257 311
Size: 364 582 3174
Fragments arranged by size:
3174 582 364
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4120
FspI TGC'GCA
Cuts at: 247 1899 247
Size: 1652 2468
HaeII r_GCGC'y
Cuts at: 254 322 438 783 826 1552 1800 2824 3803
Size: 68 116 345 43 726 248 1024 979
Cuts at: 3803 254
Size: 571
Fragments arranged by size:
1024 979 726 571 345 248 116 68
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1960 1990 1992 2022 1960
Size: 30 2 30 4058
Fragments arranged by size:
4058 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4120
KasI G'GCGC_C
Cuts at: 318 822 1548 1796 318
Size: 504 726 248 2642
Fragments arranged by size:
2642 726 504 248
KpnI G_GTAC'C
Cuts at: 599 1138 599
Size: 539 3581
MluI A'CGCG_T
Cuts at: 3553 3553
Size: 4120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2791 2975 2791
Size: 184 3936
MscI TGG'CCA
Cuts at: 484 1879 484
Size: 1395 2725
MslI CAynn'nnrTG
Cuts at: 2107 2234 2107
Size: 127 3993
MspA1I CmG'CkG
Cuts at: 307 459 736 763 811 1229 1543 1903 2918
Size: 152 277 27 48 418 314 360 1015
Cuts at: 2918 3163 307
Size: 245 1264
Fragments arranged by size:
1264 1015 418 360 314 277 245 152
48 27
NaeI GCC'GGC
Cuts at: 350 369 2299 350
Size: 19 1930 2171
Fragments arranged by size:
2171 1930 19
NarI GG'CG_CC
Cuts at: 319 823 1549 1797 319
Size: 504 726 248 2642
Fragments arranged by size:
2642 726 504 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4120
NgoMIV G'CCGG_C
Cuts at: 348 367 2297 348
Size: 19 1930 2171
Fragments arranged by size:
2171 1930 19
NruI TCG'CGA
Cuts at: 2481 2481
Size: 4120
NspI r_CATG'y
Cuts at: 1171 1671 2580 1171
Size: 500 909 2711
Fragments arranged by size:
2711 909 500
PciI A'CATG_T
Cuts at: 2576 2576
Size: 4120
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3291 3259
Size: 32 4088
PpuMI rG'GwC_Cy
Cuts at: 311 675 311
Size: 364 3756
PshAI GACnn'nnGTC
Cuts at: 496 496
Size: 4120
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3435
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4120
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3760
RsrII CG'GwC_CG
Cuts at: 1018 2313 1018
Size: 1295 2825
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4120
SacII CC_GC'GG
Cuts at: 460 764 460
Size: 304 3816
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4120
SanDI GG'GwC_CC
Cuts at: 311 311
Size: 4120
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3910
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4120
ScaI AGT'ACT
Cuts at: 3637 3637
Size: 4120
SfcI C'TryA_G
Cuts at: 33 1161 1846 2841 3032 33
Size: 1128 685 995 191 1121
Fragments arranged by size:
1128 1121 995 685 191
SfoI GGC'GCC
Cuts at: 320 824 1550 1798 320
Size: 504 726 248 2642
Fragments arranged by size:
2642 726 504 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4120
SmaI CCC'GGG
Cuts at: 342 981 1038 1140 342
Size: 639 57 102 3322
Fragments arranged by size:
3322 639 102 57
SmlI C'TyrA_G
Cuts at: 841 1273 2188 2682 2944 841
Size: 432 915 494 262 2017
Fragments arranged by size:
2017 915 494 432 262
SnaBI TAC'GTA
Cuts at: 1110 1110
Size: 4120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4120
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3620
SrfI GCCC'GGGC
Cuts at: 1038 1038
Size: 4120
StyI C'CwwG_G
Cuts at: 125 663 1252 1606 125
Size: 538 589 354 2639
Fragments arranged by size:
2639 589 538 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 1701 1834 2293 2504 433
Size: 1268 133 459 211 2049
Fragments arranged by size:
2049 1268 459 211 133
TatI w'GTAC_w
Cuts at: 3635 3635
Size: 4120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3637 3669 3637
Size: 32 4088
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4120
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 368 1613 368
Size: 1245 2875
XmaI C'CCGG_G
Cuts at: 340 979 1036 1138 340
Size: 639 57 102 3322
Fragments arranged by size:
3322 639 102 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SspI StuI SwaI XhoI XmnI ZraI