Restriction map for pF1KSDB0021
 (Linear) (Six-base) MAPSORT of: KIBB0021.seq  Check: 8647  from: 1  to: 981



KIBB0021.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AccI GT'mk_AC

Cuts at:      0     137     981 
   Size:        137     844
 
Acc65I G'GTAC_C

Cuts at:      0     469     981 
   Size:        469     512
 
AfeI AGC'GCT

Cuts at:      0     310     655     981 
   Size:        310     345     326

  Fragments arranged by size: 

                 345     326     310
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     103     981 
   Size:        103     878
 
AvaI C'yCGr_G

Cuts at:      0     214     496     831     853     910     981 
   Size:        214     282     335      22      57      71

  Fragments arranged by size: 

                 335     282     214      71      57      22
 
AvrII C'CTAG_G

Cuts at:      0     537     981 
   Size:        537     444
 
BanI G'GyrC_C

Cuts at:      0     192     348     469     696     981 
   Size:        192     156     121     227     285

  Fragments arranged by size: 

                 285     227     192     156     121
 
BanII G_rGCy'C

Cuts at:      0     623     911     981 
   Size:        623     288      70

  Fragments arranged by size: 

                 623     288      70
 
BbeI G_GCGC'C

Cuts at:      0     196     700     981 
   Size:        196     504     281

  Fragments arranged by size: 

                 504     281     196
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     483     981 
   Size:        483     498
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     951     981 
   Size:        951      30
 
BglI GCCn_nnn'nGGC

Cuts at:      0     527     539     686     857     973     981 
   Size:        527      12     147     171     116       8

  Fragments arranged by size: 

                 527     171     147     116      12       8
 
BglII A'GATC_T

Cuts at:      0      50     917     981 
   Size:         50     867      64

  Fragments arranged by size: 

                 867      64      50
 
Bme1580I G_kGCm'C

Cuts at:      0     157     646     981 
   Size:        157     489     335

  Fragments arranged by size: 

                 489     335     157
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     330     981 
   Size:        330     651
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     389     441     978     981 
   Size:         34     355      52     537       3

  Fragments arranged by size: 

                 537     355      52      34       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     142     981 
   Size:        142     839
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700     981 
   Size:        700     281
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     111     283     778     981 
   Size:        111     172     495     203

  Fragments arranged by size: 

                 495     203     172     111
 
BsaHI Gr'CG_yC

Cuts at:      0     193     488     697     981 
   Size:        193     295     209     284

  Fragments arranged by size: 

                 295     284     209     193
 
BsaWI w'CCGG_w

Cuts at:      0     894     981 
   Size:        894      87
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     303     770     790     889     898     981 
   Size:        303     467      20      99       9      83

  Fragments arranged by size: 

                 467     303      99      83      20       9
 
BseYI C'CCAG_C

Cuts at:      0     181     245     482     681     981 
   Size:        181      64     237     199     300

  Fragments arranged by size: 

                 300     237     199     181      64
 
BsiEI CG_ry'CG

Cuts at:      0     230     569     981 
   Size:        230     339     412

  Fragments arranged by size: 

                 412     339     230
 
BsiHKAI G_wGCw'C

Cuts at:      0     964     981 
   Size:        964      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     324     981 
   Size:        324     657
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     623     646     911     964     981 
   Size:        157     466      23     265      53      17

  Fragments arranged by size: 

                 466     265     157      53      23      17
 
BspEI T'CCGG_A

Cuts at:      0     894     981 
   Size:        894      87
 
BsrBI CCG'CTC

Cuts at:      0     271     714     890     981 
   Size:        271     443     176      91

  Fragments arranged by size: 

                 443     271     176      91
 
BsrFI r'CCGG_y

Cuts at:      0     172     222     241     351     564     933     981 
   Size:        172      50      19     110     213     369      48

  Fragments arranged by size: 

                 369     213     172     110      50      48      19
 
BssHII G'CGCG_C

Cuts at:      0     514     596     981 
   Size:        514      82     385

  Fragments arranged by size: 

                 514     385      82
 
BssSI C'ACGA_G

Cuts at:      0     272     506     981 
   Size:        272     234     475

  Fragments arranged by size: 

                 475     272     234
 
BstYI r'GATC_y

Cuts at:      0      50     917     981 
   Size:         50     867      64

  Fragments arranged by size: 

                 867      64      50
 
BtgI C'CryG_G

Cuts at:      0     331     359     635     981 
   Size:        331      28     276     346

  Fragments arranged by size: 

                 346     331     276      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       7     981 
   Size:          7     974
 
EaeI y'GGCC_r

Cuts at:      0     170     227     283     356     452     566     981 
   Size:        170      57      56      73      96     114     415

  Fragments arranged by size: 

                 415     170     114      96      73      57      56
 
EagI C'GGCC_G

Cuts at:      0     227     566     981 
   Size:        227     339     415

  Fragments arranged by size: 

                 415     339     227
 
EarI CTCTTCn'nnn_

Cuts at:      0     866     981 
   Size:        866     115
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     407     415     981 
   Size:        407       8     566

  Fragments arranged by size: 

                 566     407       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     389     441     978     981 
   Size:         34     355      52     537       3

  Fragments arranged by size: 

                 537     355      52      34       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     185     549     981 
   Size:        185     364     432

  Fragments arranged by size: 

                 432     364     185
 
FspI TGC'GCA

Cuts at:      0     121     981 
   Size:        121     860
 
HaeII r_GCGC'y

Cuts at:      0     128     196     312     657     700     981 
   Size:        128      68     116     345      43     281

  Fragments arranged by size: 

                 345     281     128     116      68      43
 
KasI G'GCGC_C

Cuts at:      0     192     696     981 
   Size:        192     504     285

  Fragments arranged by size: 

                 504     285     192
 
KpnI G_GTAC'C

Cuts at:      0     473     981 
   Size:        473     508
 
MscI TGG'CCA

Cuts at:      0     358     981 
   Size:        358     623
 
MspA1I CmG'CkG

Cuts at:      0     181     333     610     637     685     981 
   Size:        181     152     277      27      48     296

  Fragments arranged by size: 

                 296     277     181     152      48      27
 
NaeI GCC'GGC

Cuts at:      0     224     243     981 
   Size:        224      19     738

  Fragments arranged by size: 

                 738     224      19
 
NarI GG'CG_CC

Cuts at:      0     193     697     981 
   Size:        193     504     284

  Fragments arranged by size: 

                 504     284     193
 
NgoMIV G'CCGG_C

Cuts at:      0     222     241     981 
   Size:        222      19     740

  Fragments arranged by size: 

                 740     222      19
 
PpuMI rG'GwC_Cy

Cuts at:      0     185     549     981 
   Size:        185     364     432

  Fragments arranged by size: 

                 432     364     185
 
PshAI GACnn'nnGTC

Cuts at:      0     370     981 
   Size:        370     611
 
RsrII CG'GwC_CG

Cuts at:      0     892     981 
   Size:        892      89
 
SacII CC_GC'GG

Cuts at:      0     334     638     981 
   Size:        334     304     343

  Fragments arranged by size: 

                 343     334     304
 
SanDI GG'GwC_CC

Cuts at:      0     185     981 
   Size:        185     796
 
SfoI GGC'GCC

Cuts at:      0     194     698     981 
   Size:        194     504     283

  Fragments arranged by size: 

                 504     283     194
 
SmaI CCC'GGG

Cuts at:      0     216     855     912     981 
   Size:        216     639      57      69

  Fragments arranged by size: 

                 639     216      69      57
 
SmlI C'TyrA_G

Cuts at:      0     715     981 
   Size:        715     266
 
SrfI GCCC'GGGC

Cuts at:      0     912     981 
   Size:        912      69
 
StyI C'CwwG_G

Cuts at:      0     537     981 
   Size:        537     444
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     307     981 
   Size:        307     674
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     242     981 
   Size:        242     739
 
XmaI C'CCGG_G

Cuts at:      0     214     853     910     981 
   Size:        214     639      57      71

  Fragments arranged by size: 

                 639     214      71      57
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI     AlfI     AvaI    AvrII     BanI    BanII
     BbeI     BbsI     BcgI     BglI    BglII Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII    BssSI
    BstYI     BtgI    BtgZI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I     FspI    HaeII     KasI     KpnI     MscI   MspA1I     NaeI
     NarI   NgoMIV    PpuMI    PshAI    RsrII    SacII    SanDI     SfoI
     SmaI     SmlI     SrfI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI     BlpI
    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaXI     BsgI    BsiWI
     BsmI    BspHI    BspMI    BsrDI    BsrGI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     MfeI     MluI     MmeI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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