Restriction map for pF1KSDB0017
 (Linear) (Six-base) MAPSORT of: KIBB0017.seq  Check: 5777  from: 1  to: 5220



KIBB0017.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     648    3213    5220 
   Size:        648    2565    2007

  Fragments arranged by size: 

                2565    2007     648
 
AatII G_ACGT'C

Cuts at:      0    2848    5220 
   Size:       2848    2372
 
AccI GT'mk_AC

Cuts at:      0    2366    4745    5220 
   Size:       2366    2379     475

  Fragments arranged by size: 

                2379    2366     475
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334    1090    1341    1696    2137    2781    4570    5220 
   Size:        334     756     251     355     441     644    1789     650

  Fragments arranged by size: 

                1789     756     650     644     441     355     334     251
            
 
AfeI AGC'GCT

Cuts at:      0    3586    3767    5220 
   Size:       3586     181    1453

  Fragments arranged by size: 

                3586    1453     181
 
AflIII A'CryG_T

Cuts at:      0     824    1750    4592    5220 
   Size:        824     926    2842     628

  Fragments arranged by size: 

                2842     926     824     628
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1404    4734    5220 
   Size:       1404    3330     486

  Fragments arranged by size: 

                3330    1404     486
 
AleI CACnn'nnGTG

Cuts at:      0    1753    2396    4891    5220 
   Size:       1753     643    2495     329

  Fragments arranged by size: 

                2495    1753     643     329
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1408    1569    1849    5220 
   Size:       1408     161     280    3371

  Fragments arranged by size: 

                3371    1408     280     161
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1264    1296    4597    4629    5220 
   Size:       1264      32    3301      32     591

  Fragments arranged by size: 

                3301    1264     591      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     663    1332    1341    1533    3315    3558    3942    3978 
   Size:        663     669       9     192    1782     243     384      36

Cuts at:   3978    5220 
   Size:       1242

  Fragments arranged by size: 

                1782    1242     669     663     384     243     192      36
                   9
 
ApaI G_GGCC'C

Cuts at:      0    2805    3240    5220 
   Size:       2805     435    1980

  Fragments arranged by size: 

                2805    1980     435
 
ApaLI G'TGCA_C

Cuts at:      0    5101    5220 
   Size:       5101     119
 
ApoI r'AATT_y

Cuts at:      0     214     523     553    5220 
   Size:        214     309      30    4667

  Fragments arranged by size: 

                4667     309     214      30
 
AseI AT'TA_AT

Cuts at:      0    2317    5220 
   Size:       2317    2903
 
AvaI C'yCGr_G

Cuts at:      0    1370    1489    2782    3120    3160    3239    3386    3964 
   Size:       1370     119    1293     338      40      79     147     578

Cuts at:   3964    3991    4095    4120    4172    5220 
   Size:         27     104      25      52    1048

  Fragments arranged by size: 

                1370    1293    1048     578     338     147     119     104
                  79      52      40      27      25
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2830    2863    5220 
   Size:       2830      33    2357

  Fragments arranged by size: 

                2830    2357      33
 
BanI G'GyrC_C

Cuts at:      0     793    1375    2016    2254    2696    3189    3286    3382 
   Size:        793     582     641     238     442     493      97      96

Cuts at:   3382    3630    4777    5220 
   Size:        248    1147     443

  Fragments arranged by size: 

                1147     793     641     582     493     443     442     248
                 238      97      96
 
BanII G_rGCy'C

Cuts at:      0     363     755     918    1058    1139    2805    2933    3154 
   Size:        363     392     163     140      81    1666     128     221

Cuts at:   3154    3240    3740    4033    4475    5150    5220 
   Size:         86     500     293     442     675      70

  Fragments arranged by size: 

                1666     675     500     442     392     363     293     221
                 163     140     128      86      81      70
 
BbeI G_GCGC'C

Cuts at:      0    1379    2020    2258    3193    3290    5220 
   Size:       1379     641     238     935      97    1930

  Fragments arranged by size: 

                1930    1379     935     641     238      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     407    1687    1978    2961    3268    3412    4109    4644 
   Size:        407    1280     291     983     307     144     697     535

Cuts at:   4644    5220 
   Size:        576

  Fragments arranged by size: 

                1280     983     697     576     535     407     307     291
                 144
 
BbvCI CC'TCA_GC

Cuts at:      0    3375    5220 
   Size:       3375    1845
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3855    3889    5220 
   Size:       3855      34    1331

  Fragments arranged by size: 

                3855    1331      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2831    5220 
   Size:       2831    2389
 
BglI GCCn_nnn'nGGC

Cuts at:      0     276    2515    3574    3646    4090    4721    5220 
   Size:        276    2239    1059      72     444     631     499

  Fragments arranged by size: 

                2239    1059     631     499     444     276      72
 
BlpI GC'TnA_GC

Cuts at:      0    3514    3581    4027    5220 
   Size:       3514      67     446    1193

  Fragments arranged by size: 

                3514    1193     446      67
 
Bme1580I G_kGCm'C

Cuts at:      0    2054    2805    3240    3431    5105    5220 
   Size:       2054     751     435     191    1674     115

  Fragments arranged by size: 

                2054    1674     751     435     191     115
 
BmgBI CAC'GTC

Cuts at:      0    1401    2673    3297    4017    4593    4686    5220 
   Size:       1401    1272     624     720     576      93     534

  Fragments arranged by size: 

                1401    1272     720     624     576     534      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      70    1521    3303    4151    4283    5220 
   Size:         70    1451    1782     848     132     937

  Fragments arranged by size: 

                1782    1451     937     848     132      70
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1977    3168    3390    4515    5220 
   Size:       1977    1191     222    1125     705

  Fragments arranged by size: 

                1977    1191    1125     705     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     439    1042    1099    2256    2260    2395    2551 
   Size:        304     135     603      57    1157       4     135     156

Cuts at:   2551    2947    3138    3223    3317    3523    3801    5123    5220 
   Size:        396     191      85      94     206     278    1322      97

  Fragments arranged by size: 

                1322    1157     603     396     304     278     206     191
                 156     135     135      97      94      85      57       4
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1138    3375    3943    5220 
   Size:       1138    2237     568    1277

  Fragments arranged by size: 

                2237    1277    1138     568
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     654    1211    2820    3005    5220 
   Size:        654     557    1609     185    2215

  Fragments arranged by size: 

                2215    1609     654     557     185
 
BsaAI yAC'GTr

Cuts at:      0    4809    5220 
   Size:       4809     411
 
BsaHI Gr'CG_yC

Cuts at:      0     668    1376    2017    2255    2845    3190    3287    4232 
   Size:        668     708     641     238     590     345      97     945

Cuts at:   4232    5220 
   Size:        988

  Fragments arranged by size: 

                 988     945     708     668     641     590     345     238
                  97
 
BsaWI w'CCGG_w

Cuts at:      0     173     620    2711    3464    4756    5220 
   Size:        173     447    2091     753    1292     464

  Fragments arranged by size: 

                2091    1292     753     464     447     173
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1165    1195    1264    1294    1648    1678    1989    2019 
   Size:       1165      30      69      30     354      30     311      30

Cuts at:   2019    2524    2554    2799    2829    2956    2986    3118    3148 
   Size:        505      30     245      30     127      30     132      30

Cuts at:   3148    3667    3697    3817    3847    4171    4201    4738    4768 
   Size:        519      30     120      30     324      30     537      30

Cuts at:   4768    5220 
   Size:        452

  Fragments arranged by size: 

                1165     537     519     505     452     354     324     311
                 245     132     127     120      69      30      30      30
                  30      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     893    1189    1456    1459    1982    3516    3650    3715 
   Size:        893     296     267       3     523    1534     134      65

Cuts at:   3715    3841    3862    3879    3948    4387    5181    5220 
   Size:        126      21      17      69     439     794      39

  Fragments arranged by size: 

                1534     893     794     523     439     296     267     134
                 126      69      65      39      21      17       3
 
BseYI C'CCAG_C

Cuts at:      0    2040    3214    3446    5086    5220 
   Size:       2040    1174     232    1640     134

  Fragments arranged by size: 

                2040    1640    1174     232     134
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346    1861    3332    3418    4411    4878    4933    5114 
   Size:        346    1515    1471      86     993     467      55     181

Cuts at:   5114    5220 
   Size:        106

  Fragments arranged by size: 

                1515    1471     993     467     346     181     106      86
                  55
 
BsiEI CG_ry'CG

Cuts at:      0     254     280    3103    4387    5220 
   Size:        254      26    2823    1284     833

  Fragments arranged by size: 

                2823    1284     833     254      26
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     755    1058    1139    2165    2203    2933    3154 
   Size:        227     528     303      81    1026      38     730     221

Cuts at:   3154    3599    4475    5105    5220 
   Size:        445     876     630     115

  Fragments arranged by size: 

                1026     876     730     630     528     445     303     227
                 221     115      81      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3891    5220 
   Size:       3891    1329
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     363     755     918    1058    1139    2054    2165 
   Size:        227     136     392     163     140      81     915     111

Cuts at:   2165    2203    2805    2933    3154    3240    3431    3599    3740 
   Size:         38     602     128     221      86     191     168     141

Cuts at:   3740    4033    4475    5105    5150    5220 
   Size:        293     442     630      45      70

  Fragments arranged by size: 

                 915     630     602     442     392     293     227     221
                 191     168     163     141     140     136     128     111
                  86      81      70      45      38
 
BspEI T'CCGG_A

Cuts at:      0     173     620    2711    3464    5220 
   Size:        173     447    2091     753    1756

  Fragments arranged by size: 

                2091    1756     753     447     173
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     648    1340    3213    3791    4928    5220 
   Size:        648     692    1873     578    1137     292

  Fragments arranged by size: 

                1873    1137     692     648     578     292
 
BsrBI CCG'CTC

Cuts at:      0     276    1610    2244    3513    4151    4383    4985    5220 
   Size:        276    1334     634    1269     638     232     602     235

  Fragments arranged by size: 

                1334    1269     638     634     602     276     235     232
            
 
BsrFI r'CCGG_y

Cuts at:      0     693     920    3192    3300    3633    3902    4022    4535 
   Size:        693     227    2272     108     333     269     120     513

Cuts at:   4535    5220 
   Size:        685

  Fragments arranged by size: 

                2272     693     685     513     333     269     227     120
                 108
 
BsrGI T'GTAC_A

Cuts at:      0    1724    3614    5220 
   Size:       1724    1890    1606

  Fragments arranged by size: 

                1890    1724    1606
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     300    3978    5220 
   Size:        300    3678    1242

  Fragments arranged by size: 

                3678    1242     300
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4686    5220 
   Size:       4686     534
 
BstYI r'GATC_y

Cuts at:      0     258    3455    4406    5057    5220 
   Size:        258    3197     951     651     163

  Fragments arranged by size: 

                3197     951     651     258     163
 
Bsu36I CC'TnA_GG

Cuts at:      0    4034    5220 
   Size:       4034    1186
 
BtgI C'CryG_G

Cuts at:      0     122     671     977    1166    1200    2294    2582    2888 
   Size:        122     549     306     189      34    1094     288     306

Cuts at:   2888    3231    3270    3340    3689    5220 
   Size:        343      39      70     349    1531

  Fragments arranged by size: 

                1531    1094     549     349     343     306     306     288
                 189     122      70      39      34
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     567    4644    5220 
   Size:        567    4077     576

  Fragments arranged by size: 

                4077     576     567
 
BtsI GCAGTG_nn'

Cuts at:      0     236     502    1873    2089    2190    2524    3327    4861 
   Size:        236     266    1371     216     101     334     803    1534

Cuts at:   4861    5220 
   Size:        359

  Fragments arranged by size: 

                1534    1371     803     359     334     266     236     216
                 101
 
DraIII CAC_nnn'GTG

Cuts at:      0     231     300    5220 
   Size:        231      69    4920

  Fragments arranged by size: 

                4920     231      69
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     646     673    1507    5220 
   Size:        646      27     834    3713

  Fragments arranged by size: 

                3713     834     646      27
 
EaeI y'GGCC_r

Cuts at:      0       2     277    1307    1520    1649    2291    2516    2549 
   Size:          2     275    1030     213     129     642     225      33

Cuts at:   2549    3194    3647    3900    4608    4895    5220 
   Size:        645     453     253     708     287     325

  Fragments arranged by size: 

                1030     708     645     642     453     325     287     275
                 253     225     213     129      33       2
 
EagI C'GGCC_G

Cuts at:      0     277    5220 
   Size:        277    4943
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     370     467    2071    2989    5220 
   Size:        215     155      97    1604     918    2231

  Fragments arranged by size: 

                2231    1604     918     215     155      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3466    3675    5020    5038    5220 
   Size:       3466     209    1345      18     182

  Fragments arranged by size: 

                3466    1345     209     182      18
 
EcoICRI GAG'CTC

Cuts at:      0     753    1056    1137    2931    3152    4473    5220 
   Size:        753     303      81    1794     221    1321     747

  Fragments arranged by size: 

                1794    1321     753     747     303     221      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     334     439    1042    1090    1099    1341    1696 
   Size:        304      30     105     603      48       9     242     355

Cuts at:   1696    2137    2256    2260    2395    2551    2781    2947    3138 
   Size:        441     119       4     135     156     230     166     191

Cuts at:   3138    3223    3317    3523    3801    4570    5123    5220 
   Size:         85      94     206     278     769     553      97

  Fragments arranged by size: 

                 769     603     553     441     355     304     278     242
                 230     206     191     166     156     135     119     105
                  97      94      85      48      30       9       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     838    1546    1997    2377    3798    5220 
   Size:        838     708     451     380    1421    1422

  Fragments arranged by size: 

                1422    1421     838     708     451     380
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351     432    2002    2026    2382    2742    2801    3095 
   Size:        351      81    1570      24     356     360      59     294

Cuts at:   3095    3236    3996    4061    4126    4875    5220 
   Size:        141     760      65      65     749     345

  Fragments arranged by size: 

                1570     760     749     360     356     351     345     294
                 141      81      65      65      59      24
 
EcoRI G'AATT_C

Cuts at:      0     523    5220 
   Size:        523    4697
 
EcoRV GAT'ATC

Cuts at:      0    5001    5220 
   Size:       5001     219
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2811    5220 
   Size:       2811    2409
 
FseI GG_CCGG'CC

Cuts at:      0    3906    5220 
   Size:       3906    1314
 
FspI TGC'GCA

Cuts at:      0    1384    5220 
   Size:       1384    3836
 
FspAI rTGC'GCAy

Cuts at:      0    1384    5220 
   Size:       1384    3836
 
HaeII r_GCGC'y

Cuts at:      0     815    1342    1379    2020    2258    2467    3193    3290 
   Size:        815     527      37     641     238     209     726      97

Cuts at:   3290    3588    3769    4716    5184    5220 
   Size:        298     181     947     468      36

  Fragments arranged by size: 

                 947     815     726     641     527     468     298     238
                 209     181      97      37      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      63      95     591     623     985    1017    1390    1422 
   Size:         63      32     496      32     362      32     373      32

Cuts at:   1422    2524    2556    2799    2831    2869    2901    3118    3150 
   Size:       1102      32     243      32      38      32     217      32

Cuts at:   3150    3817    3849    4357    4389    4720    4752    5220 
   Size:        667      32     508      32     331      32     468

  Fragments arranged by size: 

                1102     667     508     496     468     373     362     331
                 243     217      63      38      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1163    5220 
   Size:       1163    4057
 
HindIII A'AGCT_T

Cuts at:      0    3061    4771    5220 
   Size:       3061    1710     449

  Fragments arranged by size: 

                3061    1710     449
 
KasI G'GCGC_C

Cuts at:      0    1375    2016    2254    3189    3286    5220 
   Size:       1375     641     238     935      97    1934

  Fragments arranged by size: 

                1934    1375     935     641     238      97
 
MluI A'CGCG_T

Cuts at:      0    1750    5220 
   Size:       1750    3470
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    2999    3698    3728    4214    4428    5012    5105 
   Size:        419    2580     699      30     486     214     584      93

Cuts at:   5105    5220 
   Size:        115

  Fragments arranged by size: 

                2580     699     584     486     419     214     115      93
                  30
 
MscI TGG'CCA

Cuts at:      0       4    1522    2551    4897    5220 
   Size:          4    1518    1029    2346     323

  Fragments arranged by size: 

                2346    1518    1029     323       4
 
MslI CAynn'nnrTG

Cuts at:      0     175    1390    1753    2396    2680    4669    4891    5220 
   Size:        175    1215     363     643     284    1989     222     329

  Fragments arranged by size: 

                1989    1215     643     363     329     284     222     175
            
 
MspA1I CmG'CkG

Cuts at:      0     124     282     711    1979    2196    2396    2584    3379 
   Size:        124     158     429    1268     217     200     188     795

Cuts at:   3379    3488    4700    4824    5055    5175    5220 
   Size:        109    1212     124     231     120      45

  Fragments arranged by size: 

                1268    1212     795     429     231     217     200     188
                 158     124     124     120     109      45
 
NaeI GCC'GGC

Cuts at:      0     695    3194    3302    3635    3904    4024    4537    5220 
   Size:        695    2499     108     333     269     120     513     683

  Fragments arranged by size: 

                2499     695     683     513     333     269     120     108
            
 
NarI GG'CG_CC

Cuts at:      0    1376    2017    2255    3190    3287    5220 
   Size:       1376     641     238     935      97    1933

  Fragments arranged by size: 

                1933    1376     935     641     238      97
 
NcoI C'CATG_G

Cuts at:      0     977    3270    5220 
   Size:        977    2293    1950

  Fragments arranged by size: 

                2293    1950     977
 
NgoMIV G'CCGG_C

Cuts at:      0     693    3192    3300    3633    3902    4022    4535    5220 
   Size:        693    2499     108     333     269     120     513     685

  Fragments arranged by size: 

                2499     693     685     513     333     269     120     108
            
 
NotI GC'GGCC_GC

Cuts at:      0     277    5220 
   Size:        277    4943
 
NruI TCG'CGA

Cuts at:      0    1819    5220 
   Size:       1819    3401
 
NspI r_CATG'y

Cuts at:      0     828     986    3172    3546    5220 
   Size:        828     158    2186     374    1674

  Fragments arranged by size: 

                2186    1674     828     374     158
 
PasI CC'CwG_GG

Cuts at:      0    3441    3742    4000    5220 
   Size:       3441     301     258    1220

  Fragments arranged by size: 

                3441    1220     301     258
 
PciI A'CATG_T

Cuts at:      0     824    5220 
   Size:        824    4396
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     837     978    1065    1122    1533    3044    3315    5220 
   Size:        837     141      87      57     411    1511     271    1905

  Fragments arranged by size: 

                1905    1511     837     411     271     141      87      57
            
 
PfoI T'CCnGG_A

Cuts at:      0    3333    5220 
   Size:       3333    1887
 
PmlI CAC'GTG

Cuts at:      0    4809    5220 
   Size:       4809     411
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1264    1296    5220 
   Size:       1264      32    3924

  Fragments arranged by size: 

                3924    1264      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     351    3095    3996    4061    4126    5220 
   Size:        351    2744     901      65      65    1094

  Fragments arranged by size: 

                2744    1094     901     351      65      65
 
PshAI GACnn'nnGTC

Cuts at:      0    1147    1687    5220 
   Size:       1147     540    3533

  Fragments arranged by size: 

                3533    1147     540
 
PspOMI G'GGCC_C

Cuts at:      0    2801    3236    5220 
   Size:       2801     435    1984

  Fragments arranged by size: 

                2801    1984     435
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4501    4533    5220 
   Size:       4501      32     687

  Fragments arranged by size: 

                4501     687      32
 
PstI C_TGCA'G

Cuts at:      0      93     497     659    1337    1838    2025    2084    2434 
   Size:         93     404     162     678     501     187      59     350

Cuts at:   2434    2877    3134    3554    3974    3983    4307    4856    5220 
   Size:        443     257     420     420       9     324     549     364

  Fragments arranged by size: 

                 678     549     501     443     420     420     404     364
                 350     324     257     187     162      93      59       9
            
 
PvuII CAG'CTG

Cuts at:      0    2196    3379    3488    5220 
   Size:       2196    1183     109    1732

  Fragments arranged by size: 

                2196    1732    1183     109
 
RsrII CG'GwC_CG

Cuts at:      0     623    5220 
   Size:        623    4597
 
SacI G_AGCT'C

Cuts at:      0     755    1058    1139    2933    3154    4475    5220 
   Size:        755     303      81    1794     221    1321     745

  Fragments arranged by size: 

                1794    1321     755     745     303     221      81
 
SacII CC_GC'GG

Cuts at:      0     125    2585    5220 
   Size:        125    2460    2635

  Fragments arranged by size: 

                2635    2460     125
 
SanDI GG'GwC_CC

Cuts at:      0    3095    3996    4061    4126    5220 
   Size:       3095     901      65      65    1094

  Fragments arranged by size: 

                3095    1094     901      65      65
 
SapI GCTCTTCn'nnn_

Cuts at:      0     215     467    2071    5220 
   Size:        215     252    1604    3149

  Fragments arranged by size: 

                3149    1604     252     215
 
SbfI CC_TGCA'GG

Cuts at:      0     659    1337    2025    3554    5220 
   Size:        659     678     688    1529    1666

  Fragments arranged by size: 

                1666    1529     688     678     659
 
ScaI AGT'ACT

Cuts at:      0    2811    5220 
   Size:       2811    2409
 
SexAI A'CCwGG_T

Cuts at:      0    1505    4841    5220 
   Size:       1505    3336     379

  Fragments arranged by size: 

                3336    1505     379
 
SfcI C'TryA_G

Cuts at:      0      89     493     655    1284    1333    1834    2021    2080 
   Size:         89     404     162     629      49     501     187      59

Cuts at:   2080    2430    2568    2873    3130    3550    3795    3970    3979 
   Size:        350     138     305     257     420     245     175       9

Cuts at:   3979    4303    4503    4852    5220 
   Size:        324     200     349     368

  Fragments arranged by size: 

                 629     501     420     404     368     350     349     324
                 305     257     245     200     187     175     162     138
                  89      59      49       9
 
SfoI GGC'GCC

Cuts at:      0    1377    2018    2256    3191    3288    5220 
   Size:       1377     641     238     935      97    1932

  Fragments arranged by size: 

                1932    1377     935     641     238      97
 
SmaI CCC'GGG

Cuts at:      0    3162    3241    3388    5220 
   Size:       3162      79     147    1832

  Fragments arranged by size: 

                3162    1832     147      79
 
SpeI A'CTAG_T

Cuts at:      0    2812    5220 
   Size:       2812    2408
 
StuI AGG'CCT

Cuts at:      0    1254    1840    3113    3373    4586    5220 
   Size:       1254     586    1273     260    1213     634

  Fragments arranged by size: 

                1273    1254    1213     634     586     260
 
StyI C'CwwG_G

Cuts at:      0     789     977    1121    1310    1667    3182    3270    4679 
   Size:        789     188     144     189     357    1515      88    1409

Cuts at:   4679    5220 
   Size:        541

  Fragments arranged by size: 

                1515    1409     789     541     357     189     188     144
                  88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     811    1540    4438    5220 
   Size:        811     729    2898     782

  Fragments arranged by size: 

                2898     811     782     729
 
TatI w'GTAC_w

Cuts at:      0     578    1724    2648    2809    3614    5220 
   Size:        578    1146     924     161     805    1606

  Fragments arranged by size: 

                1606    1146     924     805     578     161
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1165    1197    1989    2021    2182    2214    3375    3407 
   Size:       1165      32     792      32     161      32    1161      32

Cuts at:   3407    4819    4851    5095    5127    5220 
   Size:       1412      32     244      32      93

  Fragments arranged by size: 

                1412    1165    1161     792     244     161      93      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     487     627    3099    5220 
   Size:        487     140    2472    2121

  Fragments arranged by size: 

                2472    2121     487     140
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1530    2433    2511    2862    3045    4683    5094    5220 
   Size:       1530     903      78     351     183    1638     411     126

  Fragments arranged by size: 

                1638    1530     903     411     351     183     126      78
            
 
XmaI C'CCGG_G

Cuts at:      0    3160    3239    3386    5220 
   Size:       3160      79     147    1834

  Fragments arranged by size: 

                3160    1834     147      79
 
XmnI GAAnn'nnTTC

Cuts at:      0     404     527    1195    5220 
   Size:        404     123     668    4025

  Fragments arranged by size: 

                4025     668     404     123
 
ZraI GAC'GTC

Cuts at:      0    2846    5220 
   Size:       2846    2374
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     KasI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PsrI     PstI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI
     SpeI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AscI    AsiSI    AvrII    BamHI
     BclI    BfrBI    BglII     BmtI    BpuEI    BsaBI    BsiWI     BsmI
    BspHI    BsrDI   BssHII    BssSI    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     HpaI     KpnI     MfeI     NdeI     NheI     NsiI
     PacI     PmeI     PsiI    PspXI     PvuI     SalI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SphI     SrfI     SspI     SwaI     XbaI
     XhoI
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