Restriction map for pF1KSDB0008
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspI        
AvrII       BalI        BamHI       BbeI        BcgI        
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BspMI       BsrFI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraII       DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XcmI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       1321  GGTACC               G^GTACC
AhdI          444  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1531  GTGCAC               G^TGCAC
AocI          457  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1531  GTGCAC               G^TGCAC
Asp700I      1827  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1321  GGTACC               G^GTACC
AspEI         444  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         978  TGCGCA               TGC^GCA
BanI         1321  GGTACC               G^G[CT][GA]CC
BbrPI        1280  CACGTG               CAC^GTG
BbvCI         658  GCTGAGG              CCTCAGC(-5/-2)
BglI            9  GCCCAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1280  CACGTG               [CT]AC^GT[GA]
BsmBI         805  CGTCTC               CGTCTC(1/5)
Bsp1407I      524  TGTACA               T^GTACA
BsrBI         414  CCGCTC               CCGCTC(-3/-3)
BsrDI        1336  GCAATG               GCAATG(2/0)
BsrGI         524  TGTACA               T^GTACA
BssSI         718  CACGAG               CACGAG(-5/-1)
BstYI         320  AGATCC               [GA]^GATC[CT]
Bsu36I        457  CCTGAGG              CC^T[ACGT]AGG
CfoI          979  GCGC                 GCG^C
DraI         1207  TTTAAA               TTT^AAA
DraIII       1277  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      444  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        457  CCTGAGG              CC^T[ACGT]AGG
FspI          978  TGCGCA               TGC^GCA
HhaI          979  GCGC                 GCG^C
HinP1I        979  GCGC                 G^CGC
HincII         78  GTTGAC               GT[CT]^[GA]AC
HindII         78  GTTGAC               GT[CT]^[GA]AC
KpnI         1321  GGTACC               GGTAC^C
MflI          320  AGATCC               [GA]^GATC[CT]
NcoI         1646  CCATGG               C^CATGG
PaeR7I       1111  CTCGAG               C^TCGAG
PmaCI        1280  CACGTG               CAC^GTG
PmlI         1280  CACGTG               CAC^GTG
SacI           94  GAGCTC               GAGCT^C
ScaI         1781  AGTACT               AGT^ACT
SmaI         1664  CCCGGG               CCC^GGG
SmlI         1111  CTCGAG               C^T[CT][GA]AG
SspBI         524  TGTACA               T^GTACA
StuI         1886  AGGCCT               AGG^CCT
XhoI         1111  CTCGAG               C^TCGAG
XhoII         320  AGATCC               [GA]^GATC[CT]
XmaCI        1664  CCCGGG               C^CCGGG
XmaI         1664  CCCGGG               C^CCGGG
XmnI         1827  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI           86  AAATTC               [GA]^AATT[CT]
             1204  GAATTT              
AlwI          321  GATCC                GGATC(4/5)
             1135  GATCC               
ApoI           86  AAATTC               [GA]^AATT[CT]
             1204  GAATTT              
AvaI         1111  CTCGAG               C^[CT]CG[GA]G
             1664  CCCGGG              
BanII          94  GAGCTC               G[GA]GC[CT]^C
              310  GAGCCC              
BciVI         427  GTATCC               GTATCC(6/5)
             1142  GTATCC              
BfaI         1357  CTAG                 C^TAG
             1466  CTAG                
BsaI         1763  GAGACC               GGTCTC(1/5)
               31  GGTCTC              
BsoBI        1111  CTCGAG               C^[CT]CG[GA]G
             1664  CCCGGG              
BspHI        1344  TCATGA               T^CATGA
             1626  TCATGA              
DsaI          821  CCGTGG               C^C[GA][CT]GG
             1646  CCATGG              
MaeI         1357  CTAG                 C^TAG
             1466  CTAG                
MaeIII        404  GTGAC                ^GT[ACGT]AC
             1016  GTCAC               
PleI          839  GACTC                GAGTC(4/5)
             1341  GACTC               
RcaI         1344  TCATGA               T^CATGA
             1626  TCATGA              
SapI          745  GAAGAGC              GCTCTTC(1/4)
             1000  GAAGAGC             
SfaNI         712  GATGC                GCATC(5/9)
             1860  GATGC               
Tsp45I        404  GTGAC                ^GT[GC]AC
             1016  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     Van91I      
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