Restriction map for pF1KSDB0008
 (Linear) (Six-base) MAPSORT of: KIBB0008.seq  Check: 8441  from: 1  to: 1893



KIBB0008.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

Acc65I G'GTAC_C

Cuts at:      0    1321    1893 
   Size:       1321     572
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764     926    1090    1893 
   Size:        764     162     164     803

  Fragments arranged by size: 

                 803     764     164     162
 
AhdI GACnn_n'nnGTC

Cuts at:      0     449    1893 
   Size:        449    1444
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366     765    1155    1893 
   Size:        366     399     390     738

  Fragments arranged by size: 

                 738     399     390     366
 
ApaLI G'TGCA_C

Cuts at:      0    1531    1893 
   Size:       1531     362
 
ApoI r'AATT_y

Cuts at:      0      86    1204    1893 
   Size:         86    1118     689

  Fragments arranged by size: 

                1118     689      86
 
AvaI C'yCGr_G

Cuts at:      0    1111    1664    1893 
   Size:       1111     553     229

  Fragments arranged by size: 

                1111     553     229
 
BanI G'GyrC_C

Cuts at:      0    1321    1893 
   Size:       1321     572
 
BanII G_rGCy'C

Cuts at:      0      98     314    1893 
   Size:         98     216    1579

  Fragments arranged by size: 

                1579     216      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     448    1078    1358    1893 
   Size:        448     630     280     535

  Fragments arranged by size: 

                 630     535     448     280
 
BbvCI CC'TCA_GC

Cuts at:      0     659    1893 
   Size:        659    1234
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     438    1153    1893 
   Size:        438     715     740

  Fragments arranged by size: 

                 740     715     438
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15    1893 
   Size:         15    1878
 
Bme1580I G_kGCm'C

Cuts at:      0    1535    1893 
   Size:       1535     358
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     426    1893 
   Size:        426    1467
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1653    1893 
   Size:       1653     240
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     328    1287    1893 
   Size:        112     216     959     606

  Fragments arranged by size: 

                 959     606     216     112
 
Bpu10I CC'TnA_GC

Cuts at:      0     659    1893 
   Size:        659    1234
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37    1757    1893 
   Size:         37    1720     136

  Fragments arranged by size: 

                1720     136      37
 
BsaAI yAC'GTr

Cuts at:      0    1282    1893 
   Size:       1282     611
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     580     811    1240    1855    1893 
   Size:        580     231     429     615      38

  Fragments arranged by size: 

                 615     580     429     231      38
 
BseYI C'CCAG_C

Cuts at:      0     109     935    1893 
   Size:        109     826     958

  Fragments arranged by size: 

                 958     826     109
 
BsiHKAI G_wGCw'C

Cuts at:      0      98    1164    1278    1535    1893 
   Size:         98    1066     114     257     358

  Fragments arranged by size: 

                1066     358     257     114      98
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     811    1893 
   Size:        811    1082
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     314    1164    1278    1535    1893 
   Size:         98     216     850     114     257     358

  Fragments arranged by size: 

                 850     358     257     216     114      98
 
BspHI T'CATG_A

Cuts at:      0    1344    1626    1893 
   Size:       1344     282     267

  Fragments arranged by size: 

                1344     282     267
 
BsrBI CCG'CTC

Cuts at:      0     416    1893 
   Size:        416    1477
 
BsrDI GCAATG_nn'

Cuts at:      0    1343    1893 
   Size:       1343     550
 
BsrGI T'GTAC_A

Cuts at:      0     524    1893 
   Size:        524    1369
 
BssSI C'ACGA_G

Cuts at:      0     718    1893 
   Size:        718    1175
 
BstYI r'GATC_y

Cuts at:      0     320    1893 
   Size:        320    1573
 
Bsu36I CC'TnA_GG

Cuts at:      0     458    1893 
   Size:        458    1435
 
BtgI C'CryG_G

Cuts at:      0     821    1646    1893 
   Size:        821     825     247

  Fragments arranged by size: 

                 825     821     247
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     928    1893 
   Size:        928     965
 
BtsI GCAGTG_nn'

Cuts at:      0    1535    1893 
   Size:       1535     358
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     803     838    1893 
   Size:        803      35    1055

  Fragments arranged by size: 

                1055     803      35
 
DraI TTT'AAA

Cuts at:      0    1209    1893 
   Size:       1209     684
 
DraIII CAC_nnn'GTG

Cuts at:      0    1282    1893 
   Size:       1282     611
 
EarI CTCTTCn'nnn_

Cuts at:      0     389     740     995    1489    1755    1893 
   Size:        389     351     255     494     266     138

  Fragments arranged by size: 

                 494     389     351     266     255     138
 
EcoICRI GAG'CTC

Cuts at:      0      96    1893 
   Size:         96    1797
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     328     764     926    1090    1287    1893 
   Size:        112     216     436     162     164     197     606

  Fragments arranged by size: 

                 606     436     216     197     164     162     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     748    1405    1569    1893 
   Size:        748     657     164     324

  Fragments arranged by size: 

                 748     657     324     164
 
FspI TGC'GCA

Cuts at:      0     980    1893 
   Size:        980     913
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     435     467     568     600     874     906     978    1010 
   Size:        435      32     101      32     274      32      72      32

Cuts at:   1010    1893 
   Size:        883

  Fragments arranged by size: 

                 883     435     274     101      72      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0      80    1893 
   Size:         80    1813
 
KpnI G_GTAC'C

Cuts at:      0    1325    1893 
   Size:       1325     568
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     517    1893 
   Size:        517    1376
 
MspA1I CmG'CkG

Cuts at:      0     109     306     742     904    1893 
   Size:        109     197     436     162     989

  Fragments arranged by size: 

                 989     436     197     162     109
 
NcoI C'CATG_G

Cuts at:      0    1646    1893 
   Size:       1646     247
 
PasI CC'CwG_GG

Cuts at:      0      11    1893 
   Size:         11    1882
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     330     834    1785    1893 
   Size:        330     504     951     108

  Fragments arranged by size: 

                 951     504     330     108
 
PfoI T'CCnGG_A

Cuts at:      0      35    1893 
   Size:         35    1858
 
PmlI CAC'GTG

Cuts at:      0    1282    1893 
   Size:       1282     611
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1765    1797    1893 
   Size:       1765      32      96

  Fragments arranged by size: 

                1765      96      32
 
PvuII CAG'CTG

Cuts at:      0     109     306     742     904    1893 
   Size:        109     197     436     162     989

  Fragments arranged by size: 

                 989     436     197     162     109
 
SacI G_AGCT'C

Cuts at:      0      98    1893 
   Size:         98    1795
 
SapI GCTCTTCn'nnn_

Cuts at:      0     740     995    1893 
   Size:        740     255     898

  Fragments arranged by size: 

                 898     740     255
 
ScaI AGT'ACT

Cuts at:      0    1783    1893 
   Size:       1783     110
 
SfcI C'TryA_G

Cuts at:      0     380     698    1147    1240    1408    1893 
   Size:        380     318     449      93     168     485

  Fragments arranged by size: 

                 485     449     380     318     168      93
 
SmaI CCC'GGG

Cuts at:      0    1666    1893 
   Size:       1666     227
 
SmlI C'TyrA_G

Cuts at:      0    1111    1893 
   Size:       1111     782
 
StuI AGG'CCT

Cuts at:      0    1888    1893 
   Size:       1888       5
 
StyI C'CwwG_G

Cuts at:      0     283    1646    1871    1893 
   Size:        283    1363     225      22

  Fragments arranged by size: 

                1363     283     225      22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     264     391    1893 
   Size:        264     127    1502

  Fragments arranged by size: 

                1502     264     127
 
TatI w'GTAC_w

Cuts at:      0     524    1781    1893 
   Size:        524    1257     112

  Fragments arranged by size: 

                1257     524     112
 
XhoI C'TCGA_G

Cuts at:      0    1111    1893 
   Size:       1111     782
 
XmaI C'CCGG_G

Cuts at:      0    1664    1893 
   Size:       1664     229
 
XmnI GAAnn'nnTTC

Cuts at:      0    1831    1893 
   Size:       1831      62
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbsI    BbvCI    BciVI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrGI    BssSI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EarI  EcoICRI
  Eco57MI     FalI     FspI    Hin4I   HincII     KpnI     MmeI   MspA1I
     NcoI     PasI    PflMI     PfoI     PmlI     PsrI    PvuII     SacI
     SapI     ScaI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrFI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     ClaI     DrdI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI    FspAI    HaeII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     ZraI
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