Restriction map for pF1KSDB0004
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AhdI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaBI       BsaI        
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrDI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FspI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        ScaI        
SfuI        SnaBI       SpeI        SspI        SwaI        
Tru9I       Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        2647  GACGTC               GACGT^C
AgeI          771  ACCGGT               A^CCGGT
Aor51HI      2448  AGCGCT               AGC^GCT
AspI         2335  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         527  GGATCC               G^GATCC
BsaWI         771  ACCGGT               [AT]^CCGG[AT]
BsmAI         977  GAGAC                GTCTC(1/5)
Bsp1407I     2801  TGTACA               T^GTACA
BsrGI        2801  TGTACA               T^GTACA
BssSI        2899  CACGAG               CACGAG(-5/-1)
BstYI         527  GGATCC               [GA]^GATC[CT]
DraIII        580  CACGTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2448  AGCGCT               AGC^GCT
EcoNI         174  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI           51  GGCCGGCC             GGCCGG^CC
HincII       2017  GTTGAC               GT[CT]^[GA]AC
HindII       2017  GTTGAC               GT[CT]^[GA]AC
MflI          527  GGATCC               [GA]^GATC[CT]
PaeR7I       2808  CTCGAG               C^TCGAG
PflFI        2335  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         771  ACCGGT               A^CCGGT
PshAI        1190  GACGGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2284  GAGCTC               GAGCT^C
SapI         2487  GCTCTTC              GCTCTTC(1/4)
SbfI         1632  CCTGCAGG             CCTGCA^GG
SfaNI        2546  GCATC                GCATC(5/9)
SmlI         2808  CTCGAG               C^T[CT][GA]AG
Sse8387I     1632  CCTGCAGG             CCTGCA^GG
SspBI        2801  TGTACA               T^GTACA
TfiI         2113  GATTC                G^A[AT]TC
Tth111I      2335  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2808  CTCGAG               C^TCGAG
XhoII         527  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Alw44I       1044  GTGCAC               G^TGCAC
             2374  GTGCAC              
AlwI          528  GATCC                GGATC(4/5)
              527  GGATC               
ApaLI        1044  GTGCAC               G^TGCAC
             2374  GTGCAC              
BbeI         1741  GGCGCC               GGCGC^C
             1957  GGCGCC              
BbsI         1573  GTCTTC               GAAGAC(2/6)
             2314  GTCTTC              
BbvCI        1312  CCTCAGC              CCTCAGC(-5/-2)
             2586  CCTCAGC             
BfaI          856  CTAG                 C^TAG
             3109  CTAG                
BlpI          120  GCTCAGC              GC^T[ACGT]AGC
             1986  GCTCAGC             
Bpu1102I      120  GCTCAGC              GC^T[ACGT]AGC
             1986  GCTCAGC             
BpuAI        1573  GTCTTC               GAAGAC(2/6)
             2314  GTCTTC              
BssHII       2429  GCGCGC               G^CGCGC
             2619  GCGCGC              
BstXI        1113  CCAGGAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1781  CCACGGTGCTGG        
CelII         120  GCTCAGC              GC^T[ACGT]AGC
             1986  GCTCAGC             
KasI         1741  GGCGCC               G^GCGCC
             1957  GGCGCC              
KspI         1508  CCGCGG               CCGC^GG
             2886  CCGCGG              
MaeI          856  CTAG                 C^TAG
             3109  CTAG                
NarI         1741  GGCGCC               GG^CGCC
             1957  GGCGCC              
PleI         2353  GACTC                GAGTC(4/5)
             1492  GAGTC               
SacII        1508  CCGCGG               CCGC^GG
             2886  CCGCGG              
SfiI         1203  GGCCCAGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2255  GGCCACACTGGCC       
SfoI         1741  GGCGCC               GGC^GCC
             1957  GGCGCC              
SgrAI         770  CACCGGTG             C[GA]^CCGG[CT]G
             1953  CACCGGCG            
SphI         1367  GCATGC               GCATG^C
             2148  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SexAI       
SfcI        SmaI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com