Restriction map for pF1KSDB0004
 (Linear) (Six-base) MAPSORT of: KIBB0004.seq  Check: 2774  from: 1  to: 3258



KIBB0004.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2282    2540    3258 
   Size:       2282     258     718

  Fragments arranged by size: 

                2282     718     258
 
AatII G_ACGT'C

Cuts at:      0    2651    3258 
   Size:       2651     607
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     678    1560    3258 
   Size:        678     882    1698

  Fragments arranged by size: 

                1698     882     678
 
AfeI AGC'GCT

Cuts at:      0    2450    3258 
   Size:       2450     808
 
AgeI A'CCGG_T

Cuts at:      0     771    3258 
   Size:        771    2487
 
AleI CACnn'nnGTG

Cuts at:      0     472    2276    3258 
   Size:        472    1804     982

  Fragments arranged by size: 

                1804     982     472
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2050    2082    3258 
   Size:       2050      32    1176

  Fragments arranged by size: 

                2050    1176      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     485    1172    1184    1320    1545    1641    3090    3182 
   Size:        485     687      12     136     225      96    1449      92

Cuts at:   3182    3258 
   Size:         76

  Fragments arranged by size: 

                1449     687     485     225     136      96      92      76
                  12
 
ApaI G_GGCC'C

Cuts at:      0     505     658    1490    3248    3258 
   Size:        505     153     832    1758      10

  Fragments arranged by size: 

                1758     832     505     153      10
 
ApaLI G'TGCA_C

Cuts at:      0    1044    2374    3258 
   Size:       1044    1330     884

  Fragments arranged by size: 

                1330    1044     884
 
AvaI C'yCGr_G

Cuts at:      0     613     831     948    1228    1236    1483    1489    2760 
   Size:        613     218     117     280       8     247       6    1271

Cuts at:   2760    2808    3258 
   Size:         48     450

  Fragments arranged by size: 

                1271     613     450     280     247     218     117      48
                   8       6
 
BamHI G'GATC_C

Cuts at:      0     527    3258 
   Size:        527    2731
 
BanI G'GyrC_C

Cuts at:      0     622     774     916    1741    1957    2077    2185    2270 
   Size:        622     152     142     825     216     120     108      85

Cuts at:   2270    2719    3258 
   Size:        449     539

  Fragments arranged by size: 

                 825     622     539     449     216     152     142     120
                 108      85
 
BanII G_rGCy'C

Cuts at:      0     290     353     428     505     658     822     832    1130 
   Size:        290      63      75      77     153     164      10     298

Cuts at:   1130    1490    2015    2288    2831    2954    3248    3258 
   Size:        360     525     273     543     123     294      10

  Fragments arranged by size: 

                 543     525     360     298     294     290     273     164
                 153     123      77      75      63      10      10
 
BbeI G_GCGC'C

Cuts at:      0    1745    1961    3258 
   Size:       1745     216    1297

  Fragments arranged by size: 

                1745    1297     216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1566    2307    3258 
   Size:       1566     741     951

  Fragments arranged by size: 

                1566     951     741
 
BbvCI CC'TCA_GC

Cuts at:      0    1313    2587    3258 
   Size:       1313    1274     671

  Fragments arranged by size: 

                1313    1274     671
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1175    1210    1353    2262    2734    2912    3258 
   Size:       1175      35     143     909     472     178     346

  Fragments arranged by size: 

                1175     909     472     346     178     143      35
 
BlpI GC'TnA_GC

Cuts at:      0     121    1987    3258 
   Size:        121    1866    1271

  Fragments arranged by size: 

                1866    1271     121
 
Bme1580I G_kGCm'C

Cuts at:      0     505     658    1048    1490    2188    2273    2378    2722 
   Size:        505     153     390     442     698      85     105     344

Cuts at:   2722    3248    3258 
   Size:        526      10

  Fragments arranged by size: 

                 698     526     505     442     390     344     153     105
                  85      10
 
BmgBI CAC'GTC

Cuts at:      0     582    3258 
   Size:        582    2676
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2709    3258 
   Size:       2709     549
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2487    3258 
   Size:       2487     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     913     993    1900    2482    2497    2677    2724    3258 
   Size:        913      80     907     582      15     180      47     534

  Fragments arranged by size: 

                 913     907     582     534     180      80      47      15
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1313    1663    2587    3258 
   Size:       1313     350     924     671

  Fragments arranged by size: 

                1313     924     671     350
 
BsaAI yAC'GTr

Cuts at:      0    1374    1595    1775    2790    2997    3258 
   Size:       1374     221     180    1015     207     261

  Fragments arranged by size: 

                1374    1015     261     221     207     180
 
BsaHI Gr'CG_yC

Cuts at:      0    1742    1958    2312    2516    2648    2861    3258 
   Size:       1742     216     354     204     132     213     397

  Fragments arranged by size: 

                1742     397     354     216     213     204     132
 
BsaWI w'CCGG_w

Cuts at:      0     771    3258 
   Size:        771    2487
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     593     623     968     998    2050    2080    2338    2368 
   Size:        593      30     345      30    1052      30     258      30

Cuts at:   2368    3258 
   Size:        890

  Fragments arranged by size: 

                1052     890     593     345     258      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     397     400     409     418     421     424    1819 
   Size:        145     252       3       9       9       3       3    1395

Cuts at:   1819    2023    3258 
   Size:        204    1235

  Fragments arranged by size: 

                1395    1235     252     204     145       9       9       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     437     639     657     758     788     836     841    1165 
   Size:        437     202      18     101      30      48       5     324

Cuts at:   1165    1328    1644    2062    2416    2840    3104    3258 
   Size:        163     316     418     354     424     264     154

  Fragments arranged by size: 

                 437     424     418     354     324     316     264     202
                 163     154     101      48      30      18       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     141     445    1087    1543    1754    1879    2422 
   Size:         18     123     304     642     456     211     125     543

Cuts at:   2422    2977    2994    3019    3061    3258 
   Size:        555      17      25      42     197

  Fragments arranged by size: 

                 642     555     543     456     304     211     197     125
                 123      42      25      18      17
 
BsiEI CG_ry'CG

Cuts at:      0      11      53     572    1693    3253    3258 
   Size:         11      42     519    1121    1560       5

  Fragments arranged by size: 

                1560    1121     519      42      11       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      70    1048    1081    2288    2378    3258 
   Size:         70     978      33    1207      90     880

  Fragments arranged by size: 

                1207     978     880      90      70      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     290     353     428     505     658     822     832 
   Size:         70     220      63      75      77     153     164      10

Cuts at:    832    1048    1081    1130    1490    2015    2188    2273    2288 
   Size:        216      33      49     360     525     173      85      15

Cuts at:   2288    2378    2722    2831    2954    3248    3258 
   Size:         90     344     109     123     294      10

  Fragments arranged by size: 

                 525     360     344     294     220     216     173     164
                 153     123     109      90      85      77      75      70
                  63      49      33      15      10      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     304    2282    2540    2929    2999    3075    3258 
   Size:        304    1978     258     389      70      76     183

  Fragments arranged by size: 

                1978     389     304     258     183      76      70
 
BsrBI CCG'CTC

Cuts at:      0     418    1689    1707    2466    2895    3216    3258 
   Size:        418    1271      18     759     429     321      42

  Fragments arranged by size: 

                1271     759     429     418     321      42      18
 
BsrFI r'CCGG_y

Cuts at:      0      52     571     771     805     934    1819    1954    3121 
   Size:         52     519     200      34     129     885     135    1167

Cuts at:   3121    3232    3258 
   Size:        111      26

  Fragments arranged by size: 

                1167     885     519     200     135     129     111      52
                  34      26
 
BsrGI T'GTAC_A

Cuts at:      0    2801    3258 
   Size:       2801     457
 
BssHII G'CGCG_C

Cuts at:      0    2429    2619    3258 
   Size:       2429     190     639

  Fragments arranged by size: 

                2429     639     190
 
BssSI C'ACGA_G

Cuts at:      0    2899    3258 
   Size:       2899     359
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1184    1545    1641    2921    3258 
   Size:       1184     361      96    1280     337

  Fragments arranged by size: 

                1280    1184     361     337      96
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1120    1788    3258 
   Size:       1120     668    1470

  Fragments arranged by size: 

                1470    1120     668
 
BstYI r'GATC_y

Cuts at:      0     527    3258 
   Size:        527    2731
 
Bsu36I CC'TnA_GG

Cuts at:      0     536     779    1351    3258 
   Size:        536     243     572    1907

  Fragments arranged by size: 

                1907     572     536     243
 
BtgI C'CryG_G

Cuts at:      0       5     344     473     523     905    1508    1781    2212 
   Size:          5     339     129      50     382     603     273     431

Cuts at:   2212    2357    2435    2519    2831    2886    3258 
   Size:        145      78      84     312      55     372

  Fragments arranged by size: 

                 603     431     382     372     339     312     273     145
                 129      84      78      55      50       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3136    3258 
   Size:       3136     122
 
BtsI GCAGTG_nn'

Cuts at:      0     370     960    1137    1276    1290    1546    1612    2310 
   Size:        370     590     177     139      14     256      66     698

Cuts at:   2310    3007    3258 
   Size:        697     251

  Fragments arranged by size: 

                 698     697     590     370     256     251     177     139
                  66      14
 
DraIII CAC_nnn'GTG

Cuts at:      0     585    3258 
   Size:        585    2673
 
EaeI y'GGCC_r

Cuts at:      0       2      50      54     341     476     569     971    1690 
   Size:          2      48       4     287     135      93     402     719

Cuts at:   1690    1886    1943    2254    2263    2423    2580    2855    3258 
   Size:        196      57     311       9     160     157     275     403

  Fragments arranged by size: 

                 719     403     402     311     287     275     196     160
                 157     135      93      57      48       9       4       2
            
 
EagI C'GGCC_G

Cuts at:      0      50     569    1690    3258 
   Size:         50     519    1121    1568

  Fragments arranged by size: 

                1568    1121     519      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     893    1837    2494    3258 
   Size:        893     944     657     764

  Fragments arranged by size: 

                 944     893     764     657
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     436    1699    3258 
   Size:        436    1263    1559

  Fragments arranged by size: 

                1559    1263     436
 
EcoICRI GAG'CTC

Cuts at:      0    2286    3258 
   Size:       2286     972
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     678     913     993    1560    1900    2482    2497    2677 
   Size:        678     235      80     567     340     582      15     180

Cuts at:   2677    2724    3258 
   Size:         47     534

  Fragments arranged by size: 

                 678     582     567     534     340     235     180      80
                  47      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178    3258 
   Size:        178    3080
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     501     540     654     684     845    2757    3087 
   Size:        255     246      39     114      30     161    1912     330

Cuts at:   3087    3200    3245    3258 
   Size:        113      45      13

  Fragments arranged by size: 

                1912     330     255     246     161     114     113      45
                  39      30      13
 
FseI GG_CCGG'CC

Cuts at:      0      56    3258 
   Size:         56    3202
 
HaeII r_GCGC'y

Cuts at:      0      63    1236    1670    1745    1885    1961    1981    2452 
   Size:         63    1173     434      75     140      76      20     471

Cuts at:   2452    2511    3258 
   Size:         59     747

  Fragments arranged by size: 

                1173     747     471     434     140      76      75      63
                  59      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     853     885    3258 
   Size:        853      32    2373

  Fragments arranged by size: 

                2373     853      32
 
HincII GTy'rAC

Cuts at:      0    2019    3258 
   Size:       2019    1239
 
KasI G'GCGC_C

Cuts at:      0    1741    1957    3258 
   Size:       1741     216    1301

  Fragments arranged by size: 

                1741    1301     216
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2270    3258 
   Size:       2270     988
 
MscI TGG'CCA

Cuts at:      0       4     343     478    1888    1945    2256    3258 
   Size:          4     339     135    1410      57     311    1002

  Fragments arranged by size: 

                1410    1002     339     311     135      57       4
 
MslI CAynn'nnrTG

Cuts at:      0     472    1372    2104    2276    2551    2959    3258 
   Size:        472     900     732     172     275     408     299

  Fragments arranged by size: 

                 900     732     472     408     299     275     172
 
MspA1I CmG'CkG

Cuts at:      0     155     299     661     762    1332    1383    1510    1848 
   Size:        155     144     362     101     570      51     127     338

Cuts at:   1848    1857    2240    2576    2642    2888    3255    3258 
   Size:          9     383     336      66     246     367       3

  Fragments arranged by size: 

                 570     383     367     362     338     336     246     155
                 144     127     101      66      51       9       3
 
NaeI GCC'GGC

Cuts at:      0      54     573     807     936    1821    3123    3258 
   Size:         54     519     234     129     885    1302     135

  Fragments arranged by size: 

                1302     885     519     234     135     129      54
 
NarI GG'CG_CC

Cuts at:      0    1742    1958    3258 
   Size:       1742     216    1300

  Fragments arranged by size: 

                1742    1300     216
 
NgoMIV G'CCGG_C

Cuts at:      0      52     571     805     934    1819    3121    3258 
   Size:         52     519     234     129     885    1302     137

  Fragments arranged by size: 

                1302     885     519     234     137     129      52
 
NspI r_CATG'y

Cuts at:      0    1371    1803    2152    3258 
   Size:       1371     432     349    1106

  Fragments arranged by size: 

                1371    1106     432     349
 
PasI CC'CwG_GG

Cuts at:      0     680     825    3258 
   Size:        680     145    2433

  Fragments arranged by size: 

                2433     680     145
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2535    2793    3144    3258 
   Size:       2535     258     351     114

  Fragments arranged by size: 

                2535     351     258     114
 
PfoI T'CCnGG_A

Cuts at:      0     551    1112    2162    2227    2492    3258 
   Size:        551     561    1050      65     265     766

  Fragments arranged by size: 

                1050     766     561     551     265      65
 
PmlI CAC'GTG

Cuts at:      0    1374    1595    1775    2790    2997    3258 
   Size:       1374     221     180    1015     207     261

  Fragments arranged by size: 

                1374    1015     261     221     207     180
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2050    2082    3258 
   Size:       2050      32    1176

  Fragments arranged by size: 

                2050    1176      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     684    2757    3087    3200    3258 
   Size:        255     429    2073     330     113      58

  Fragments arranged by size: 

                2073     429     330     255     113      58
 
PshAI GACnn'nnGTC

Cuts at:      0    1194    3258 
   Size:       1194    2064
 
PspOMI G'GGCC_C

Cuts at:      0     501     654    1486    3244    3258 
   Size:        501     153     832    1758      14

  Fragments arranged by size: 

                1758     832     501     153      14
 
PspXI vC'TCGA_Gb

Cuts at:      0    2808    3258 
   Size:       2808     450
 
PstI C_TGCA'G

Cuts at:      0     719     767     814    1637    1985    3258 
   Size:        719      48      47     823     348    1273

  Fragments arranged by size: 

                1273     823     719     348      48      47
 
PvuII CAG'CTG

Cuts at:      0     299     661     762    1857    2240    2642    3258 
   Size:        299     362     101    1095     383     402     616

  Fragments arranged by size: 

                1095     616     402     383     362     299     101
 
SacI G_AGCT'C

Cuts at:      0    2288    3258 
   Size:       2288     970
 
SacII CC_GC'GG

Cuts at:      0    1511    2889    3258 
   Size:       1511    1378     369

  Fragments arranged by size: 

                1511    1378     369
 
SanDI GG'GwC_CC

Cuts at:      0     684    2757    3200    3258 
   Size:        684    2073     443      58

  Fragments arranged by size: 

                2073     684     443      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2494    3258 
   Size:       2494     764
 
SbfI CC_TGCA'GG

Cuts at:      0    1637    3258 
   Size:       1637    1621
 
SexAI A'CCwGG_T

Cuts at:      0    1027    1697    1748    3258 
   Size:       1027     670      51    1510

  Fragments arranged by size: 

                1510    1027     670      51
 
SfcI C'TryA_G

Cuts at:      0     715     763     810    1633    1981    3258 
   Size:        715      48      47     823     348    1277

  Fragments arranged by size: 

                1277     823     715     348      48      47
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1210    2262    3258 
   Size:       1210    1052     996

  Fragments arranged by size: 

                1210    1052     996
 
SfoI GGC'GCC

Cuts at:      0    1743    1959    3258 
   Size:       1743     216    1299

  Fragments arranged by size: 

                1743    1299     216
 
SgrAI Cr'CCGG_yG

Cuts at:      0     771    1954    3258 
   Size:        771    1183    1304

  Fragments arranged by size: 

                1304    1183     771
 
SmaI CCC'GGG

Cuts at:      0     615    1238    1485    3258 
   Size:        615     623     247    1773

  Fragments arranged by size: 

                1773     623     615     247
 
SmlI C'TyrA_G

Cuts at:      0    2808    3258 
   Size:       2808     450
 
SphI G_CATG'C

Cuts at:      0    1371    2152    3258 
   Size:       1371     781    1106

  Fragments arranged by size: 

                1371    1106     781
 
SrfI GCCC'GGGC

Cuts at:      0    1238    3258 
   Size:       1238    2020
 
StuI AGG'CCT

Cuts at:      0     187    1940    2737    2986    3169    3258 
   Size:        187    1753     797     249     183      89

  Fragments arranged by size: 

                1753     797     249     187     183      89
 
StyI C'CwwG_G

Cuts at:      0     290    1409    2981    3194    3258 
   Size:        290    1119    1572     213      64

  Fragments arranged by size: 

                1572    1119     290     213      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1150    1851    2065    3151    3258 
   Size:       1150     701     214    1086     107

  Fragments arranged by size: 

                1150    1086     701     214     107
 
TatI w'GTAC_w

Cuts at:      0    2801    3258 
   Size:       2801     457
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     260     292     593     625     968    1000    1759    1791 
   Size:        260      32     301      32     343      32     759      32

Cuts at:   1791    3258 
   Size:       1467

  Fragments arranged by size: 

                1467     759     343     301     260      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2338    3258 
   Size:       2338     920
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1788    1938    2433    3258 
   Size:       1788     150     495     825

  Fragments arranged by size: 

                1788     825     495     150
 
XhoI C'TCGA_G

Cuts at:      0    2808    3258 
   Size:       2808     450
 
XmaI C'CCGG_G

Cuts at:      0     613    1236    1483    3258 
   Size:        613     623     247    1775

  Fragments arranged by size: 

                1775     623     613     247
 
ZraI GAC'GTC

Cuts at:      0    2649    3258 
   Size:       2649     609
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AgeI     AleI     AloI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FseI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AhdI     AlfI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI
    BfrBI    BglII     BmtI    BpuEI     BsaI    BsaBI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     DrdI    EcoRI    EcoRV     FalI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PsiI     PsrI
     PvuI    RsrII     SalI     ScaI     SgfI    SnaBI     SpeI     SspI
     SwaI     XbaI     XmnI
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