Restriction map for pF1KSDB0002
 (Linear) (Six-base) MAPSORT of: KIBB0002.seq  Check: 4987  from: 1  to: 3726



KIBB0002.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     705    3726 
   Size:        705    3021
 
Acc65I G'GTAC_C

Cuts at:      0     317    3726 
   Size:        317    3409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523    3726 
   Size:        523    3203
 
AflIII A'CryG_T

Cuts at:      0    2201    3726 
   Size:       2201    1525
 
AleI CACnn'nnGTG

Cuts at:      0     344    3220    3279    3334    3726 
   Size:        344    2876      59      55     392

  Fragments arranged by size: 

                2876     392     344      59      55
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     362    3364    3726 
   Size:        362    3002     362

  Fragments arranged by size: 

                3002     362     362
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1543    1575    3726 
   Size:       1543      32    2151

  Fragments arranged by size: 

                2151    1543      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     638    1304    2508    3121    3726 
   Size:        638     666    1204     613     605

  Fragments arranged by size: 

                1204     666     638     613     605
 
ApaI G_GGCC'C

Cuts at:      0     121     698    1418    2622    2899    3187    3726 
   Size:        121     577     720    1204     277     288     539

  Fragments arranged by size: 

                1204     720     577     539     288     277     121
 
ApaLI G'TGCA_C

Cuts at:      0    3582    3726 
   Size:       3582     144
 
ApoI r'AATT_y

Cuts at:      0    2698    3726 
   Size:       2698    1028
 
AscI GG'CGCG_CC

Cuts at:      0     124    3726 
   Size:        124    3602
 
AvaI C'yCGr_G

Cuts at:      0     130     263     379     697    1457    2329    2615    2621 
   Size:        130     133     116     318     760     872     286       6

Cuts at:   2621    2676    2908    2936    3218    3332    3726 
   Size:         55     232      28     282     114     394

  Fragments arranged by size: 

                 872     760     394     318     286     282     232     133
                 130     116     114      55      28       6
 
BamHI G'GATC_C

Cuts at:      0    2592    3726 
   Size:       2592    1134
 
BanI G'GyrC_C

Cuts at:      0     317     857    1000    1790    2347    3281    3726 
   Size:        317     540     143     790     557     934     445

  Fragments arranged by size: 

                 934     790     557     540     445     317     143
 
BanII G_rGCy'C

Cuts at:      0      10     121     698    1418    1909    2480    2622    2669 
   Size:         10     111     577     720     491     571     142      47

Cuts at:   2669    2677    2899    3187    3477    3726 
   Size:          8     222     288     290     249

  Fragments arranged by size: 

                 720     577     571     491     290     288     249     222
                 142     111      47      10       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1618    3726 
   Size:       1618    2108
 
BbvCI CC'TCA_GC

Cuts at:      0    3111    3451    3726 
   Size:       3111     340     275

  Fragments arranged by size: 

                3111     340     275
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     259     293     352     386    2859    2893    3726 
   Size:        259      34      59      34    2473      34     833

  Fragments arranged by size: 

                2473     833     259      59      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2449    3726 
   Size:       2449    1277
 
BclI T'GATC_A

Cuts at:      0    2709    3726 
   Size:       2709    1017
 
BglI GCCn_nnn'nGGC

Cuts at:      0     109     286     382     772    1880    2577    3091    3726 
   Size:        109     177      96     390    1108     697     514     635

  Fragments arranged by size: 

                1108     697     635     514     390     177     109      96
            
 
BglII A'GATC_T

Cuts at:      0    1545    3726 
   Size:       1545    2181
 
BlpI GC'TnA_GC

Cuts at:      0    1384    3726 
   Size:       1384    2342
 
Bme1580I G_kGCm'C

Cuts at:      0     121     464     698    1418    1504    1733    1793    1868 
   Size:        121     343     234     720      86     229      60      75

Cuts at:   1868    2352    2622    2899    2948    3187    3353    3574    3586 
   Size:        484     270     277      49     239     166     221      12

Cuts at:   3586    3726 
   Size:        140

  Fragments arranged by size: 

                 720     484     343     277     270     239     234     229
                 221     166     140     121      86      75      60      49
                  12
 
BmgBI CAC'GTC

Cuts at:      0      36     459    3726 
   Size:         36     423    3267

  Fragments arranged by size: 

                3267     423      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1617    1855    1862    2143    2882    2902    3143    3317 
   Size:       1617     238       7     281     739      20     241     174

Cuts at:   3317    3726 
   Size:        409

  Fragments arranged by size: 

                1617     739     409     281     241     238     174      20
                   7
 
BmtI G_CTAG'C

Cuts at:      0    2921    3726 
   Size:       2921     805
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1311    3327    3666    3726 
   Size:       1311    2016     339      60

  Fragments arranged by size: 

                2016    1311     339      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     969    1281    2494    2645    2764    3726 
   Size:        781     188     312    1213     151     119     962

  Fragments arranged by size: 

                1213     962     781     312     188     151     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     350    3111    3451    3726 
   Size:        350    2761     340     275

  Fragments arranged by size: 

                2761     350     340     275
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     465    3125    3648    3726 
   Size:        217     248    2660     523      78

  Fragments arranged by size: 

                2660     523     248     217      78
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     745    3726 
   Size:        745    2981
 
BsaAI yAC'GTr

Cuts at:      0    3003    3726 
   Size:       3003     723
 
BsaBI GATnn'nnATC

Cuts at:      0     541     628    3726 
   Size:        541      87    3098

  Fragments arranged by size: 

                3098     541      87
 
BsaHI Gr'CG_yC

Cuts at:      0    1953    3726 
   Size:       1953    1773
 
BsaWI w'CCGG_w

Cuts at:      0      12    1313    1338    3726 
   Size:         12    1301      25    2388

  Fragments arranged by size: 

                2388    1301      25      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1102    1132    1702    1732    3726 
   Size:       1102      30     570      30    1994

  Fragments arranged by size: 

                1994    1102     570      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1269    1390    1393    1660    1765    2356    2830    3079 
   Size:       1269     121       3     267     105     591     474     249

Cuts at:   3079    3093    3303    3726 
   Size:         14     210     423

  Fragments arranged by size: 

                1269     591     474     423     267     249     210     121
                 105      14       3
 
BseYI C'CCAG_C

Cuts at:      0     112     433     880    1014    1045    1258    1519    1999 
   Size:        112     321     447     134      31     213     261     480

Cuts at:   1999    2035    2267    2321    2479    2774    2947    3177    3508 
   Size:         36     232      54     158     295     173     230     331

Cuts at:   3508    3726 
   Size:        218

  Fragments arranged by size: 

                 480     447     331     321     295     261     232     230
                 218     213     173     158     134     112      54      36
                  31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322    1078    1464    2638    2885    3287    3726 
   Size:        322     756     386    1174     247     402     439

  Fragments arranged by size: 

                1174     756     439     402     386     322     247
 
BsiEI CG_ry'CG

Cuts at:      0     608    3726 
   Size:        608    3118
 
BsiHKAI G_wGCw'C

Cuts at:      0     275    2872    3586    3726 
   Size:        275    2597     714     140

  Fragments arranged by size: 

                2597     714     275     140
 
BsmI GAATG_Cn'

Cuts at:      0     475    3726 
   Size:        475    3251
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     121     275     464     698    1418    1504    1733 
   Size:         10     111     154     189     234     720      86     229

Cuts at:   1733    1793    1868    1909    2352    2480    2622    2669    2677 
   Size:         60      75      41     443     128     142      47       8

Cuts at:   2677    2872    2899    2948    3187    3353    3477    3574    3586 
   Size:        195      27      49     239     166     124      97      12

Cuts at:   3586    3726 
   Size:        140

  Fragments arranged by size: 

                 720     443     239     234     229     195     189     166
                 154     142     140     128     124     111      97      86
                  75      60      49      47      41      27      12      10
                   8
 
BspEI T'CCGG_A

Cuts at:      0      12    1313    1338    3726 
   Size:         12    1301      25    2388

  Fragments arranged by size: 

                2388    1301      25      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62     705    3726 
   Size:         62     643    3021

  Fragments arranged by size: 

                3021     643      62
 
BsrBI CCG'CTC

Cuts at:      0    1104    1118    1739    3361    3662    3726 
   Size:       1104      14     621    1622     301      64

  Fragments arranged by size: 

                1622    1104     621     301      64      14
 
BsrDI GCAATG_nn'

Cuts at:      0    1473    2296    3053    3726 
   Size:       1473     823     757     673

  Fragments arranged by size: 

                1473     823     757     673
 
BsrFI r'CCGG_y

Cuts at:      0     854    1070    1131    3284    3726 
   Size:        854     216      61    2153     442

  Fragments arranged by size: 

                2153     854     442     216      61
 
BssHII G'CGCG_C

Cuts at:      0      96     124    3726 
   Size:         96      28    3602

  Fragments arranged by size: 

                3602      96      28
 
BssSI C'ACGA_G

Cuts at:      0     748    1503    2574    3726 
   Size:        748     755    1071    1152

  Fragments arranged by size: 

                1152    1071     755     748
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1537    2649    3121    3726 
   Size:       1537    1112     472     605

  Fragments arranged by size: 

                1537    1112     605     472
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516     888     948    3336    3726 
   Size:        516     372      60    2388     390

  Fragments arranged by size: 

                2388     516     390     372      60
 
BstYI r'GATC_y

Cuts at:      0    1545    2305    2592    3726 
   Size:       1545     760     287    1134

  Fragments arranged by size: 

                1545    1134     760     287
 
Bsu36I CC'TnA_GG

Cuts at:      0    2258    3575    3726 
   Size:       2258    1317     151

  Fragments arranged by size: 

                2258    1317     151
 
BtgI C'CryG_G

Cuts at:      0     608    1197    1225    1368    3089    3726 
   Size:        608     589      28     143    1721     637

  Fragments arranged by size: 

                1721     637     608     589     143      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      35     267    1277    3726 
   Size:         35     232    1010    2449

  Fragments arranged by size: 

                2449    1010     232      35
 
BtsI GCAGTG_nn'

Cuts at:      0    1335    1695    1890    2948    3726 
   Size:       1335     360     195    1058     778

  Fragments arranged by size: 

                1335    1058     778     360     195
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3480    3515    3726 
   Size:       3480      35     211

  Fragments arranged by size: 

                3480     211      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     249    3325    3726 
   Size:        249    3076     401

  Fragments arranged by size: 

                3076     401     249
 
EaeI y'GGCC_r

Cuts at:      0     101     605     642     938    1154    1194    1470    1872 
   Size:        101     504      37     296     216      40     276     402

Cuts at:   1872    3726 
   Size:       1854

  Fragments arranged by size: 

                1854     504     402     296     276     216     101      40
                  37
 
EagI C'GGCC_G

Cuts at:      0     605    3726 
   Size:        605    3121
 
EarI CTCTTCn'nnn_

Cuts at:      0    1225    2430    2795    3726 
   Size:       1225    1205     365     931

  Fragments arranged by size: 

                1225    1205     931     365
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     304     781    1710    2356    3303    3726 
   Size:        304     477     929     646     947     423

  Fragments arranged by size: 

                 947     929     646     477     423     304
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     781     969    1281    2494    2645    2764    3726 
   Size:        523     258     188     312    1213     151     119     962

  Fragments arranged by size: 

                1213     962     523     312     258     188     151     119
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     427    2183    3726 
   Size:        427    1756    1543

  Fragments arranged by size: 

                1756    1543     427
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     469     513     694    1414    1415    2262    2896 
   Size:        117     352      44     181     720       1     847     634

Cuts at:   2896    3183    3193    3726 
   Size:        287      10     533

  Fragments arranged by size: 

                 847     720     634     533     352     287     181     117
                  44      10       1
 
FspI TGC'GCA

Cuts at:      0      40      58    3726 
   Size:         40      18    3668

  Fragments arranged by size: 

                3668      40      18
 
HaeII r_GCGC'y

Cuts at:      0     176     639    1088    2328    3212    3683    3726 
   Size:        176     463     449    1240     884     471      43

  Fragments arranged by size: 

                1240     884     471     463     449     176      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45    1926    1958    2407    2439    2970    3002 
   Size:         13      32    1881      32     449      32     531      32

Cuts at:   3002    3123    3155    3726 
   Size:        121      32     571

  Fragments arranged by size: 

                1881     571     531     449     121      32      32      32
                  32      32      13
 
KpnI G_GTAC'C

Cuts at:      0     321    3726 
   Size:        321    3405
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     845    2246    2264    3726 
   Size:        845    1401      18    1462

  Fragments arranged by size: 

                1462    1401     845      18
 
MscI TGG'CCA

Cuts at:      0     644    1196    1472    3726 
   Size:        644     552     276    2254

  Fragments arranged by size: 

                2254     644     552     276
 
MslI CAynn'nnrTG

Cuts at:      0     344     670    1126    1657    1823    1840    1990    2123 
   Size:        344     326     456     531     166      17     150     133

Cuts at:   2123    2200    2827    2971    2977    3220    3279    3334    3726 
   Size:         77     627     144       6     243      59      55     392

  Fragments arranged by size: 

                 627     531     456     392     344     326     243     166
                 150     144     133      77      59      55      17       6
            
 
MspA1I CmG'CkG

Cuts at:      0     314     336     404     410     604     621    1142    1370 
   Size:        314      22      68       6     194      17     521     228

Cuts at:   1370    1478    2003    2162    2542    3019    3638    3726 
   Size:        108     525     159     380     477     619      88

  Fragments arranged by size: 

                 619     525     521     477     380     314     228     194
                 159     108      88      68      22      17       6
 
NaeI GCC'GGC

Cuts at:      0     856    1133    3286    3726 
   Size:        856     277    2153     440

  Fragments arranged by size: 

                2153     856     440     277
 
NcoI C'CATG_G

Cuts at:      0    1197    3089    3726 
   Size:       1197    1892     637

  Fragments arranged by size: 

                1892    1197     637
 
NgoMIV G'CCGG_C

Cuts at:      0     854    1131    3284    3726 
   Size:        854     277    2153     442

  Fragments arranged by size: 

                2153     854     442     277
 
NheI G'CTAG_C

Cuts at:      0    2917    3726 
   Size:       2917     809
 
NspI r_CATG'y

Cuts at:      0      75     747    1839    2205    3045    3726 
   Size:         75     672    1092     366     840     681

  Fragments arranged by size: 

                1092     840     681     672     366      75
 
PasI CC'CwG_GG

Cuts at:      0    1347    1849    2586    3080    3630    3726 
   Size:       1347     502     737     494     550      96

  Fragments arranged by size: 

                1347     737     550     502     494      96
 
PciI A'CATG_T

Cuts at:      0    2201    3726 
   Size:       2201    1525
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1684    1968    3726 
   Size:       1684     284    1758

  Fragments arranged by size: 

                1758    1684     284
 
PfoI T'CCnGG_A

Cuts at:      0     758    2071    3726 
   Size:        758    1313    1655

  Fragments arranged by size: 

                1655    1313     758
 
PpuMI rG'GwC_Cy

Cuts at:      0     469    2262    3193    3726 
   Size:        469    1793     931     533

  Fragments arranged by size: 

                1793     931     533     469
 
PspOMI G'GGCC_C

Cuts at:      0     117     694    1414    2618    2895    3183    3726 
   Size:        117     577     720    1204     277     288     543

  Fragments arranged by size: 

                1204     720     577     543     288     277     117
 
PspXI vC'TCGA_Gb

Cuts at:      0     130    3726 
   Size:        130    3596
 
PstI C_TGCA'G

Cuts at:      0     409     620    1545    2513    3063    3726 
   Size:        409     211     925     968     550     663

  Fragments arranged by size: 

                 968     925     663     550     409     211
 
PvuII CAG'CTG

Cuts at:      0     314     336     404     621    2542    3019    3726 
   Size:        314      22      68     217    1921     477     707

  Fragments arranged by size: 

                1921     707     477     314     217      68      22
 
SacII CC_GC'GG

Cuts at:      0    1371    3726 
   Size:       1371    2355
 
ScaI AGT'ACT

Cuts at:      0     136    3726 
   Size:        136    3590
 
SexAI A'CCwGG_T

Cuts at:      0     245    3726 
   Size:        245    3481
 
SfcI C'TryA_G

Cuts at:      0     331     405     616    1541    2509    3059    3726 
   Size:        331      74     211     925     968     550     667

  Fragments arranged by size: 

                 968     925     667     550     331     211      74
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     772    3726 
   Size:        772    2954
 
SmaI CCC'GGG

Cuts at:      0     265     699    2331    2678    2910    2938    3220    3334 
   Size:        265     434    1632     347     232      28     282     114

Cuts at:   3334    3726 
   Size:        392

  Fragments arranged by size: 

                1632     434     392     347     282     265     232     114
                  28
 
SmlI C'TyrA_G

Cuts at:      0     130     196     444    3140    3663    3726 
   Size:        130      66     248    2696     523      63

  Fragments arranged by size: 

                2696     523     248     130      66      63
 
SphI G_CATG'C

Cuts at:      0      75    3726 
   Size:         75    3651
 
SspI AAT'ATT

Cuts at:      0    2694    3726 
   Size:       2694    1032
 
StuI AGG'CCT

Cuts at:      0    2931    3456    3726 
   Size:       2931     525     270

  Fragments arranged by size: 

                2931     525     270
 
StyI C'CwwG_G

Cuts at:      0     423     509    1197    1514    1772    2597    3089    3479 
   Size:        423      86     688     317     258     825     492     390

Cuts at:   3479    3726 
   Size:        247

  Fragments arranged by size: 

                 825     688     492     423     390     317     258     247
                  86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1393    2335    2437    3336    3726 
   Size:       1393     942     102     899     390

  Fragments arranged by size: 

                1393     942     899     390     102
 
TatI w'GTAC_w

Cuts at:      0     134    1649    2819    3726 
   Size:        134    1515    1170     907

  Fragments arranged by size: 

                1515    1170     907     134
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    1702    1734    3726 
   Size:       1102      32     568      32    1992

  Fragments arranged by size: 

                1992    1102     568      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1617    3726 
   Size:       1617    2109
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1825    2148    2559    3087    3726 
   Size:       1825     323     411     528     639

  Fragments arranged by size: 

                1825     639     528     411     323
 
XhoI C'TCGA_G

Cuts at:      0     130    3726 
   Size:        130    3596
 
XmaI C'CCGG_G

Cuts at:      0     263     697    2329    2676    2908    2936    3218    3332 
   Size:        263     434    1632     347     232      28     282     114

Cuts at:   3332    3726 
   Size:        394

  Fragments arranged by size: 

                1632     434     394     347     282     263     232     114
                  28
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AscI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI    PspXI
     PstI    PvuII    SacII     ScaI    SexAI     SfcI     SfiI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AseI
    AsiSI    AvrII     BaeI     BbeI    BfrBI    BsiWI    BsmBI    BspHI
    BsrGI    BstBI   BstEII  BstZ17I     ClaI     DraI     DrdI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NarI     NdeI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI     XbaI     XmnI     ZraI
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