Restriction map for pF1KSDA2005
 (Linear) (Six-base) MAPSORT of: KIAA2005.seq  Check: 876  from: 1  to: 4752



KIAA2005.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AccI GT'mk_AC

Cuts at:      0    2432    3225    3377    3844    4752 
   Size:       2432     793     152     467     908

  Fragments arranged by size: 

                2432     908     793     467     152
 
Acc65I G'GTAC_C

Cuts at:      0    2239    4752 
   Size:       2239    2513
 
AclI AA'CG_TT

Cuts at:      0     207    4752 
   Size:        207    4545
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1160    1550    1637    1907    2102    2268    2638    3616 
   Size:       1160     390      87     270     195     166     370     978

Cuts at:   3616    4359    4607    4752 
   Size:        743     248     145

  Fragments arranged by size: 

                1160     978     743     390     370     270     248     195
                 166     145      87
 
AflII C'TTAA_G

Cuts at:      0      79    4752 
   Size:         79    4673
 
AflIII A'CryG_T

Cuts at:      0    1436    3569    4752 
   Size:       1436    2133    1183

  Fragments arranged by size: 

                2133    1436    1183
 
AleI CACnn'nnGTG

Cuts at:      0     628    2950    4752 
   Size:        628    2322    1802

  Fragments arranged by size: 

                2322    1802     628
 
ApoI r'AATT_y

Cuts at:      0     104     336     646     687     977    1104    1624    2023 
   Size:        104     232     310      41     290     127     520     399

Cuts at:   2023    3054    3088    3345    3774    4143    4509    4752 
   Size:       1031      34     257     429     369     366     243

  Fragments arranged by size: 

                1031     520     429     399     369     366     310     290
                 257     243     232     127     104      41      34
 
AseI AT'TA_AT

Cuts at:      0      86    3172    4752 
   Size:         86    3086    1580

  Fragments arranged by size: 

                3086    1580      86
 
AvrII C'CTAG_G

Cuts at:      0    4704    4752 
   Size:       4704      48
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2934    2967    4752 
   Size:       2934      33    1785

  Fragments arranged by size: 

                2934    1785      33
 
BamHI G'GATC_C

Cuts at:      0    3930    4752 
   Size:       3930     822
 
BanI G'GyrC_C

Cuts at:      0     703    2239    2608    4752 
   Size:        703    1536     369    2144

  Fragments arranged by size: 

                2144    1536     703     369
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80     654    1427    2888    4752 
   Size:         80     574     773    1461    1864

  Fragments arranged by size: 

                1864    1461     773     574      80
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2088    4752 
   Size:       2088    2664
 
BclI T'GATC_A

Cuts at:      0     504     800    1352    2342    2669    4752 
   Size:        504     296     552     990     327    2083

  Fragments arranged by size: 

                2083     990     552     504     327     296
 
BfrBI ATG'CAT

Cuts at:      0    2256    3244    4752 
   Size:       2256     988    1508

  Fragments arranged by size: 

                2256    1508     988
 
BglII A'GATC_T

Cuts at:      0    1773    3786    4752 
   Size:       1773    2013     966

  Fragments arranged by size: 

                2013    1773     966
 
Bme1580I G_kGCm'C

Cuts at:      0    3318    4752 
   Size:       3318    1434
 
BmgBI CAC'GTC

Cuts at:      0    3319    4752 
   Size:       3319    1433
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3321    4752 
   Size:       3321    1431
 
BmtI G_CTAG'C

Cuts at:      0    1043    4752 
   Size:       1043    3709
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1693    4752 
   Size:       1693    3059
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2050    3952    4752 
   Size:       2050    1902     800

  Fragments arranged by size: 

                2050    1902     800
 
Bpu10I CC'TnA_GC

Cuts at:      0    3261    4752 
   Size:       3261    1491
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3231    3462    4752 
   Size:       3231     231    1290

  Fragments arranged by size: 

                3231    1290     231
 
BsaBI GATnn'nnATC

Cuts at:      0     362     672    4752 
   Size:        362     310    4080

  Fragments arranged by size: 

                4080     362     310
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     731     761     847     877    3699    3729    4752 
   Size:        731      30      86      30    2822      30    1023

  Fragments arranged by size: 

                2822    1023     731      86      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    3988    4752 
   Size:       3988     764
 
BseYI C'CCAG_C

Cuts at:      0    1477    2171    4752 
   Size:       1477     694    2581

  Fragments arranged by size: 

                2581    1477     694
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1946    3236    4419    4752 
   Size:       1946    1290    1183     333

  Fragments arranged by size: 

                1946    1290    1183     333
 
BsiHKAI G_wGCw'C

Cuts at:      0     567    4752 
   Size:        567    4185
 
BsmI GAATG_Cn'

Cuts at:      0    3244    4752 
   Size:       3244    1508
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3552    4752 
   Size:       3552    1200
 
Bsp1286I G_dGCh'C

Cuts at:      0     567    3318    4752 
   Size:        567    2751    1434

  Fragments arranged by size: 

                2751    1434     567
 
BspHI T'CATG_A

Cuts at:      0     252    2666    2672    4752 
   Size:        252    2414       6    2080

  Fragments arranged by size: 

                2414    2080     252       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4391    4752 
   Size:       4391     361
 
BsrDI GCAATG_nn'

Cuts at:      0     660    1169    4752 
   Size:        660     509    3583

  Fragments arranged by size: 

                3583     660     509
 
BsrGI T'GTAC_A

Cuts at:      0    1990    4752 
   Size:       1990    2762
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     108    2329    4752 
   Size:        108    2221    2423

  Fragments arranged by size: 

                2423    2221     108
 
BstEII G'GTnAC_C

Cuts at:      0    2352    3539    4752 
   Size:       2352    1187    1213

  Fragments arranged by size: 

                2352    1213    1187
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     630    1547    4752 
   Size:        630     917    3205

  Fragments arranged by size: 

                3205     917     630
 
BstYI r'GATC_y

Cuts at:      0    1671    1773    1915    2521    3786    3930    4369    4752 
   Size:       1671     102     142     606    1265     144     439     383

  Fragments arranged by size: 

                1671    1265     606     439     383     144     142     102
            
 
BstZ17I GTA'TAC

Cuts at:      0    3845    4752 
   Size:       3845     907
 
BtgI C'CryG_G

Cuts at:      0     181     623     735    1911    4067    4752 
   Size:        181     442     112    1176    2156     685

  Fragments arranged by size: 

                2156    1176     685     442     181     112
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     508    4752 
   Size:        508    4244
 
BtsI GCAGTG_nn'

Cuts at:      0    2166    4752 
   Size:       2166    2586
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     848     883    4752 
   Size:        848      35    3869

  Fragments arranged by size: 

                3869     848      35
 
DraI TTT'AAA

Cuts at:      0    1109    1304    1835    4752 
   Size:       1109     195     531    2917

  Fragments arranged by size: 

                2917    1109     531     195
 
DraIII CAC_nnn'GTG

Cuts at:      0    2824    4752 
   Size:       2824    1928
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     907    4752 
   Size:        907    3845
 
EaeI y'GGCC_r

Cuts at:      0    1058    4154    4303    4752 
   Size:       1058    3096     149     449

  Fragments arranged by size: 

                3096    1058     449     149
 
EarI CTCTTCn'nnn_

Cuts at:      0    1139    1656    3044    3075    3116    4752 
   Size:       1139     517    1388      31      41    1636

  Fragments arranged by size: 

                1636    1388    1139     517      41      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1160    1550    1637    1907    2050    2102    2268    2638 
   Size:       1160     390      87     270     143      52     166     370

Cuts at:   2638    3616    3952    4359    4607    4752 
   Size:        978     336     407     248     145

  Fragments arranged by size: 

                1160     978     407     390     370     336     270     248
                 166     145     143      87      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     557    4752 
   Size:        557    4195
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159    4273    4723    4752 
   Size:        159    4114     450      29

  Fragments arranged by size: 

                4114     450     159      29
 
EcoRI G'AATT_C

Cuts at:      0     687    4752 
   Size:        687    4065
 
EcoRV GAT'ATC

Cuts at:      0     384    1053    4752 
   Size:        384     669    3699

  Fragments arranged by size: 

                3699     669     384
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     792    2298    3628    4712    4752 
   Size:        792    1506    1330    1084      40

  Fragments arranged by size: 

                1506    1330    1084     792      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1330    1362    1440    1472    3523    3555    3699    3731 
   Size:       1330      32      78      32    2051      32     144      32

Cuts at:   3731    4752 
   Size:       1021

  Fragments arranged by size: 

                2051    1330    1021     144      78      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2742    4752 
   Size:       2742    2010
 
HindIII A'AGCT_T

Cuts at:      0    1370    2149    2874    3179    4752 
   Size:       1370     779     725     305    1573

  Fragments arranged by size: 

                1573    1370     779     725     305
 
KpnI G_GTAC'C

Cuts at:      0    2243    4752 
   Size:       2243    2509
 
MfeI C'AATT_G

Cuts at:      0     478    4752 
   Size:        478    4274
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    3383    4752 
   Size:       1310    2073    1369

  Fragments arranged by size: 

                2073    1369    1310
 
MscI TGG'CCA

Cuts at:      0    1060    4156    4305    4752 
   Size:       1060    3096     149     447

  Fragments arranged by size: 

                3096    1060     447     149
 
MslI CAynn'nnrTG

Cuts at:      0     628     640     791     970    1545    2389    2671    2950 
   Size:        628      12     151     179     575     844     282     279

Cuts at:   2950    4752 
   Size:       1802

  Fragments arranged by size: 

                1802     844     628     575     282     279     179     151
                  12
 
MspA1I CmG'CkG

Cuts at:      0    1481    4752 
   Size:       1481    3271
 
NcoI C'CATG_G

Cuts at:      0     181     735    4067    4752 
   Size:        181     554    3332     685

  Fragments arranged by size: 

                3332     685     554     181
 
NdeI CA'TA_TG

Cuts at:      0    3680    4734    4752 
   Size:       3680    1054      18

  Fragments arranged by size: 

                3680    1054      18
 
NheI G'CTAG_C

Cuts at:      0    1039    4752 
   Size:       1039    3713
 
NsiI A_TGCA'T

Cuts at:      0    2258    3246    4752 
   Size:       2258     988    1506

  Fragments arranged by size: 

                2258    1506     988
 
NspI r_CATG'y

Cuts at:      0      55    1440    2260    3573    4398    4752 
   Size:         55    1385     820    1313     825     354

  Fragments arranged by size: 

                1385    1313     825     820     354      55
 
PasI CC'CwG_GG

Cuts at:      0    2861    4752 
   Size:       2861    1891
 
PciI A'CATG_T

Cuts at:      0    1436    3569    4752 
   Size:       1436    2133    1183

  Fragments arranged by size: 

                2133    1436    1183
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2233    4752 
   Size:       2233    2519
 
PfoI T'CCnGG_A

Cuts at:      0     258    2027    2524    3968    4752 
   Size:        258    1769     497    1444     784

  Fragments arranged by size: 

                1769    1444     784     497     258
 
PpuMI rG'GwC_Cy

Cuts at:      0     159    4273    4752 
   Size:        159    4114     479

  Fragments arranged by size: 

                4114     479     159
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2886    2918    4752 
   Size:       2886      32    1834

  Fragments arranged by size: 

                2886    1834      32
 
PstI C_TGCA'G

Cuts at:      0     143     884    4106    4752 
   Size:        143     741    3222     646

  Fragments arranged by size: 

                3222     741     646     143
 
PvuII CAG'CTG

Cuts at:      0    1481    4752 
   Size:       1481    3271
 
SbfI CC_TGCA'GG

Cuts at:      0     143    4752 
   Size:        143    4609
 
ScaI AGT'ACT

Cuts at:      0    4486    4752 
   Size:       4486     266
 
SfcI C'TryA_G

Cuts at:      0     139     880    2358    3420    4102    4604    4752 
   Size:        139     741    1478    1062     682     502     148

  Fragments arranged by size: 

                1478    1062     741     682     502     148     139
 
SmlI C'TyrA_G

Cuts at:      0      79    3210    3477    4752 
   Size:         79    3131     267    1275

  Fragments arranged by size: 

                3131    1275     267      79
 
SpeI A'CTAG_T

Cuts at:      0    2851    4752 
   Size:       2851    1901
 
SspI AAT'ATT

Cuts at:      0     354    2195    2831    4752 
   Size:        354    1841     636    1921

  Fragments arranged by size: 

                1921    1841     636     354
 
StyI C'CwwG_G

Cuts at:      0     181     735     859    2588    4067    4704    4752 
   Size:        181     554     124    1729    1479     637      48

  Fragments arranged by size: 

                1729    1479     637     554     181     124      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1874    3583    4752 
   Size:       1874    1709    1169

  Fragments arranged by size: 

                1874    1709    1169
 
TatI w'GTAC_w

Cuts at:      0    1990    2854    2981    4385    4484    4752 
   Size:       1990     864     127    1404      99     268

  Fragments arranged by size: 

                1990    1404     864     268     127      99
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1810    1842    1955    1987    4752 
   Size:       1810      32     113      32    2765

  Fragments arranged by size: 

                2765    1810     113      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1884    4256    4752 
   Size:       1884    2372     496

  Fragments arranged by size: 

                2372    1884     496
 
XbaI T'CTAG_A

Cuts at:      0    1126    1533    4752 
   Size:       1126     407    3219

  Fragments arranged by size: 

                3219    1126     407
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3678    4752 
   Size:       3678    1074
 
XmnI GAAnn'nnTTC

Cuts at:      0     401     875    2281    2898    3058    4752 
   Size:        401     474    1406     617     160    1694

  Fragments arranged by size: 

                1694    1406     617     474     401     160
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AleI     ApoI
     AseI    AvrII     BaeI    BamHI     BanI     BbsI    BciVI     BclI
    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI
     DraI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NsiI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI     PsrI     PstI
    PvuII     SbfI     ScaI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AlfI     AloI    AlwNI
     ApaI    ApaLI     AscI    AsiSI     AvaI    BanII     BbeI    BbvCI
     BcgI     BglI     BlpI     BsaI    BsaAI    BsaHI    BsaWI    BsiEI
    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstBI   Bsu36I
     ClaI     EagI     EciI  EcoICRI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MluI     NaeI     NarI   NgoMIV     NotI     NruI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com