Restriction map for pF1KSDA2003
 (Linear) (Six-base) MAPSORT of: KIAA2003.seq  Check: 1821  from: 1  to: 1311



KIAA2003.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     277    1311 
   Size:        277    1034
 
ApaLI G'TGCA_C

Cuts at:      0    1242    1311 
   Size:       1242      69
 
ApoI r'AATT_y

Cuts at:      0    1188    1298    1311 
   Size:       1188     110      13

  Fragments arranged by size: 

                1188     110      13
 
BamHI G'GATC_C

Cuts at:      0     889    1311 
   Size:        889     422
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     238    1311 
   Size:        238    1073
 
Bme1580I G_kGCm'C

Cuts at:      0      37    1246    1311 
   Size:         37    1209      65

  Fragments arranged by size: 

                1209      65      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     344     728     812    1064    1232    1311 
   Size:        344     384      84     252     168      79

  Fragments arranged by size: 

                 384     344     252     168      84      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     572     992    1092    1260    1311 
   Size:        151     421     420     100     168      51

  Fragments arranged by size: 

                 421     420     168     151     100      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      91    1311 
   Size:         91    1220
 
BsaBI GATnn'nnATC

Cuts at:      0     164    1311 
   Size:        164    1147
 
BsaHI Gr'CG_yC

Cuts at:      0      22    1311 
   Size:         22    1289
 
BsaWI w'CCGG_w

Cuts at:      0     620    1311 
   Size:        620     691
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     758     759     843     927    1010    1011    1095 
   Size:        222     536       1      84      84      83       1      84

Cuts at:   1095    1179    1311 
   Size:         84     132

  Fragments arranged by size: 

                 536     222     132      84      84      84      84      83
                   1       1
 
BsiHKAI G_wGCw'C

Cuts at:      0    1246    1311 
   Size:       1246      65
 
BsmI GAATG_Cn'

Cuts at:      0     421    1311 
   Size:        421     890
 
Bsp1286I G_dGCh'C

Cuts at:      0      37    1246    1311 
   Size:         37    1209      65

  Fragments arranged by size: 

                1209      65      37
 
BspHI T'CATG_A

Cuts at:      0     648    1311 
   Size:        648     663
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     244     277    1311 
   Size:        244      33    1034

  Fragments arranged by size: 

                1034     244      33
 
BsrDI GCAATG_nn'

Cuts at:      0     213    1311 
   Size:        213    1098
 
BstYI r'GATC_y

Cuts at:      0     889    1311 
   Size:        889     422
 
BtsI GCAGTG_nn'

Cuts at:      0     495     632     747     831    1083    1167    1311 
   Size:        495     137     115      84     252      84     144

  Fragments arranged by size: 

                 495     252     144     137     115      84      84
 
EaeI y'GGCC_r

Cuts at:      0       8      56     296    1311 
   Size:          8      48     240    1015

  Fragments arranged by size: 

                1015     240      48       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     572     992    1092    1260    1311 
   Size:        151     421     420     100     168      51

  Fragments arranged by size: 

                 421     420     168     151     100      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      94    1311 
   Size:         94    1217
 
EcoRI G'AATT_C

Cuts at:      0    1298    1311 
   Size:       1298      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      12      44    1311 
   Size:         12      32    1267

  Fragments arranged by size: 

                1267      32      12
 
MscI TGG'CCA

Cuts at:      0     298    1311 
   Size:        298    1013
 
MslI CAynn'nnrTG

Cuts at:      0     653     735    1311 
   Size:        653      82     576

  Fragments arranged by size: 

                 653     576      82
 
MspA1I CmG'CkG

Cuts at:      0       7     428    1311 
   Size:          7     421     883

  Fragments arranged by size: 

                 883     421       7
 
NdeI CA'TA_TG

Cuts at:      0     484     732    1311 
   Size:        484     248     579

  Fragments arranged by size: 

                 579     484     248
 
PsiI TTA'TAA

Cuts at:      0     986    1311 
   Size:        986     325
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     450     482    1311 
   Size:        450      32     829

  Fragments arranged by size: 

                 829     450      32
 
PstI C_TGCA'G

Cuts at:      0     274     639    1311 
   Size:        274     365     672

  Fragments arranged by size: 

                 672     365     274
 
SfcI C'TryA_G

Cuts at:      0     270     467     519     635    1101    1311 
   Size:        270     197      52     116     466     210

  Fragments arranged by size: 

                 466     270     210     197     116      52
 
StuI AGG'CCT

Cuts at:      0     813     897     981    1065    1311 
   Size:        813      84      84      84     246

  Fragments arranged by size: 

                 813     246      84      84      84
 
StyI C'CwwG_G

Cuts at:      0     183    1311 
   Size:        183    1128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     874     906    1311 
   Size:        874      32     405

  Fragments arranged by size: 

                 874     405      32
 
XbaI T'CTAG_A

Cuts at:      0     156    1311 
   Size:        156    1155
 
XmnI GAAnn'nnTTC

Cuts at:      0     289    1311 
   Size:        289    1022
 

 Enzymes that do cut:

     AarI    ApaLI     ApoI    BamHI     BbsI Bme1580I     BmrI     BpmI
     BsaI    BsaBI    BsaHI    BsaWI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BstYI     BtsI     EaeI  Eco57MI    EcoNI
    EcoRI    Hin4I     MscI     MslI   MspA1I     NdeI     PsiI     PsrI
     PstI     SfcI     StuI     StyI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
  Tth111I     XcmI     XhoI     XmaI     ZraI
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