Restriction map for pF1KSDA1991
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccIII      AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
Hin1I       HpaI        KasI        KpnI        MluI        
MluNI       MroI        MscI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AclI          719  AACGTT               AA^CGTT
Aor51HI       416  AGCGCT               AGC^GCT
AspHI         515  GAGCAC               G[AT]GC[AT]^C
AspI         1957  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1286  GGATCC               G^GATCC
BanI          209  GGTGCC               G^G[CT][GA]CC
BclI         1503  TGATCA               T^GATCA
BglII        1307  AGATCT               A^GATCT
BseRI        1172  CTCCTC               GAGGAG(10/8)
BsgI          728  CTGCAC               GTGCAG(16/14)
BsiEI         184  CGGCCG               CG[GA][CT]^CG
BsiHKAI       515  GAGCAC               G[AT]GC[AT]^C
BsrDI        1112  GCAATG               GCAATG(2/0)
Bst1107I     1476  GTATAC               GTA^TAC
BstXI        2102  CCAGCAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1476  GTATAC               GTA^TAC
EagI          184  CGGCCG               C^GGCCG
EclXI         184  CGGCCG               C^GGCCG
Eco47III      416  AGCGCT               AGC^GCT
Eco52I        184  CGGCCG               C^GGCCG
EcoNI         760  CCTTCTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1503  TGATCA               T^GATCA
HaeII         416  AGCGCT               [GA]GCGC^[CT]
MfeI         1774  CAATTG               C^AATTG
MslI          945  CACAACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1774  CAATTG               C^AATTG
NspI         2033  GCATGT               [GA]CATG^[CT]
PaeR7I        764  CTCGAG               C^TCGAG
PflFI        1957  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1463  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I      719  AACGTT               AA^CGTT
SfiI           67  GGCCGGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          300  CCCGGG               CCC^GGG
Tth111I      1957  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1928  CCAGTGGGGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          764  CTCGAG               C^TCGAG
XmaCI         300  CCCGGG               C^CCGGG
XmaI          300  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1043  GTCTAC               GT^[AC][GT]AC
             1476  GTATAC              
BbsI         1779  GAAGAC               GAAGAC(2/6)
             2002  GAAGAC              
BciVI         642  GGATAC               GTATCC(6/5)
             1403  GTATCC              
BpuAI        1779  GAAGAC               GAAGAC(2/6)
             2002  GAAGAC              
BsaBI        1498  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2083  GATCAGTATC          
BsmFI         434  GGGAC                GGGAC(10/14)
             1618  GGGAC               
BsrBI          50  CCGCTC               CCGCTC(-3/-3)
             1318  GAGCGG              
BsrFI          68  GCCGGC               [GA]^CCGG[CT]
             2063  GCCGGC              
BstF5I       1064  CATCC                GGATG(2/0)
              669  GGATG               
Cfr10I         68  GCCGGC               [GA]^CCGG[CT]
             2063  GCCGGC              
FokI         1064  CATCC                GGATG(9/13)
              669  GGATG               
HincII        990  GTCAAC               GT[CT]^[GA]AC
             1656  GTCAAC              
HindII        990  GTCAAC               GT[CT]^[GA]AC
             1656  GTCAAC              
HindIII       564  AAGCTT               A^AGCTT
             1984  AAGCTT              
HphI         1162  TCACC                GGTGA(8/7)
             1709  TCACC               
MaeII         720  ACGT                 A^CGT
              732  ACGT                
MamI         1498  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2083  GATCAGTATC          
NaeI           68  GCCGGC               GCC^GGC
             2063  GCCGGC              
NgoMIV         68  GCCGGC               G^CCGGC
             2063  GCCGGC              
SapI          903  GAAGAGC              GCTCTTC(1/4)
             1834  GAAGAGC             
ScaI           19  AGTACT               AGT^ACT
              699  AGTACT              
SfaNI        1666  GATGC                GCATC(5/9)
             1121  GCATC               
SmlI          567  CTTGAG               C^T[CT][GA]AG
              764  CTCGAG              
TfiI          193  GAATC                G^A[AT]TC
              742  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AvaI        AvaII       BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmBI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
Ksp632I     KspI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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