Restriction map for
pF1KSDA1991
(Circular) (Six-base) MAPSORT of: pF1KSDA1991.seq Check: 3915 from: 1 to: 5263
pF1KSDA1991 5263 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1170 1603 2299 1170
Size: 433 696 4134
Fragments arranged by size:
4134 696 433
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5263
AclI AA'CG_TT
Cuts at: 846 5103 846
Size: 4257 1006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1161 1544 2098 3086 3518 4267 4535 758
Size: 403 383 554 988 432 749 268 1486
Fragments arranged by size:
1486 988 749 554 432 403 383 268
AfeI AGC'GCT
Cuts at: 544 544
Size: 5263
AflIII A'CryG_T
Cuts at: 2613 3719 4696 2613
Size: 1106 977 3180
Fragments arranged by size:
3180 1106 977
AgeI A'CCGG_T
Cuts at: 3651 3651
Size: 5263
AlwNI CAG_nnn'CTG
Cuts at: 2566 4135 2566
Size: 1569 3694
ApaI G_GGCC'C
Cuts at: 240 427 468 480 587 240
Size: 187 41 12 107 4916
Fragments arranged by size:
4916 187 107 41 12
ApaLI G'TGCA_C
Cuts at: 4033 4033
Size: 5263
ApoI r'AATT_y
Cuts at: 2265 4616 4872 2265
Size: 2351 256 2656
Fragments arranged by size:
2656 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5263
AvaI C'yCGr_G
Cuts at: 149 426 538 890 2281 149
Size: 277 112 352 1391 3131
Fragments arranged by size:
3131 1391 352 277 112
BamHI G'GATC_C
Cuts at: 1412 2286 1412
Size: 874 4389
BanI G'GyrC_C
Cuts at: 335 2277 2691 2939 2974 335
Size: 1942 414 248 35 2624
Fragments arranged by size:
2624 1942 414 248 35
BanII G_rGCy'C
Cuts at: 240 347 427 468 480 587 2275 3305 240
Size: 107 80 41 12 107 1688 1030 2198
Fragments arranged by size:
2198 1688 1030 107 107 80 41 12
BbeI G_GCGC'C
Cuts at: 2695 2943 2695
Size: 248 5015
BbsI GAAGACnn'nnnn_
Cuts at: 1912 2135 1912
Size: 223 5040
BciVI GTATCCnnnnn_n'
Cuts at: 762 1540 3159 3922 5263 762
Size: 778 1619 763 1341 762
Fragments arranged by size:
1619 1341 778 763 762
BclI T'GATC_A
Cuts at: 1629 2781 1629
Size: 1152 4111
BglI GCCn_nnn'nGGC
Cuts at: 200 309 418 200
Size: 109 109 5045
Fragments arranged by size:
5045 109 109
BglII A'GATC_T
Cuts at: 1433 2776 4815 1433
Size: 1343 2039 1881
Fragments arranged by size:
2039 1881 1343
BlpI GC'TnA_GC
Cuts at: 2373 2373
Size: 5263
Bme1580I G_kGCm'C
Cuts at: 240 427 468 480 587 2886 2979 4037 240
Size: 187 41 12 107 2299 93 1058 1466
Fragments arranged by size:
2299 1466 1058 187 107 93 41 12
BmgBI CAC'GTC
Cuts at: 859 859
Size: 5263
BmrI ACTGGGnnnn_n'
Cuts at: 242 2080 2473 2554 2889 5035 242
Size: 1838 393 81 335 2146 470
Fragments arranged by size:
2146 1838 470 393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1067 1772 361
Size: 706 705 3852
Fragments arranged by size:
3852 706 705
Bpu10I CC'TnA_GC
Cuts at: 951 951
Size: 5263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 714 2437 3316 3810 4108 714
Size: 1723 879 494 298 1869
Fragments arranged by size:
1869 1723 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4967 4967
Size: 5263
BsaAI yAC'GTr
Cuts at: 2253 2614 2253
Size: 361 4902
BsaBI GATnn'nnATC
Cuts at: 6 1628 2213 2775 4814 6
Size: 1622 585 562 2039 455
Fragments arranged by size:
2039 1622 585 562 455
BsaHI Gr'CG_yC
Cuts at: 2692 2940 3644 5140 2692
Size: 248 704 1496 2815
Fragments arranged by size:
2815 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2450 2971 3651 3925 4072 4483 2450
Size: 521 680 274 147 411 3230
Fragments arranged by size:
3230 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 931 961 1065 1095 1234 1264 931
Size: 30 104 30 139 30 4930
Fragments arranged by size:
4930 139 104 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1289 1289
Size: 5263
BseYI C'CCAG_C
Cuts at: 461 510 2686 3444 4023 4519 461
Size: 49 2176 758 579 496 1205
Fragments arranged by size:
2176 1205 758 579 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 839
Size: 5263
BsiEI CG_ry'CG
Cuts at: 104 313 2803 2849 4059 104
Size: 209 2490 46 1210 1308
Fragments arranged by size:
2490 1308 1210 209 46
BsiHKAI G_wGCw'C
Cuts at: 645 2275 3053 3243 4037 645
Size: 1630 778 190 794 1871
Fragments arranged by size:
1871 1630 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 208 865 1143 208
Size: 657 278 4328
Fragments arranged by size:
4328 657 278
Bsp1286I G_dGCh'C
Cuts at: 240 347 427 468 480 587 645 2275 2886
Size: 107 80 41 12 107 58 1630 611
Cuts at: 2886 2979 3053 3243 3305 4037 240
Size: 93 74 190 62 732 1466
Fragments arranged by size:
1630 1466 732 611 190 107 107 93
80 74 62 58 41 12
BspEI T'CCGG_A
Cuts at: 4483 4483
Size: 5263
BspHI T'CATG_A
Cuts at: 4439 4439
Size: 5263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 1578 1717 2311 2525 2827 3208 127
Size: 1451 139 594 214 302 381 2182
Fragments arranged by size:
2182 1451 594 381 302 214 139
BsrBI CCG'CTC
Cuts at: 50 178 1446 3553 3607 5093 5258 50
Size: 128 1268 2107 54 1486 165 55
Fragments arranged by size:
2107 1486 1268 165 128 55 54
BsrDI GCAATG_nn'
Cuts at: 1245 3173 1245
Size: 1928 3335
BsrFI r'CCGG_y
Cuts at: 194 2189 3259 3440 3651 194
Size: 1995 1070 181 211 1806
Fragments arranged by size:
1995 1806 1070 211 181
BssSI C'ACGA_G
Cuts at: 3532 3892 3532
Size: 360 4903
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5263
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5263
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2235
Size: 5263
BstYI r'GATC_y
Cuts at: 1 1412 1433 1694 2286 2753 2776 3111 3357
Size: 1411 21 261 592 467 23 335 246
Cuts at: 3357 4371 4457 4469 4815 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1411 1014 592 467 449 346 335 261
246 86 23 21 12
BstZ17I GTA'TAC
Cuts at: 1604 1604
Size: 5263
BtgI C'CryG_G
Cuts at: 125 161 307 450 578 1408 125
Size: 36 146 143 128 830 3980
Fragments arranged by size:
3980 830 146 143 128 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 898 3211 3392 3555 898
Size: 2313 181 163 2606
Fragments arranged by size:
2606 2313 181 163
BtsI GCAGTG_nn'
Cuts at: 1746 1998 2504 1746
Size: 252 506 4505
Fragments arranged by size:
4505 506 252
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 367 402 367
Size: 35 5228
DraI TTT'AAA
Cuts at: 2260 2260
Size: 5263
DrdI GACnn_nn'nnGTC
Cuts at: 2967 3827 2967
Size: 860 4403
EaeI y'GGCC_r
Cuts at: 310 375 489 1320 2237 2846 3020 3411 3438
Size: 65 114 831 917 609 174 391 27
Cuts at: 3438 4785 310
Size: 1347 788
Fragments arranged by size:
1347 917 831 788 609 391 174 114
65 27
EagI C'GGCC_G
Cuts at: 310 2846 310
Size: 2536 2727
EarI CTCTTCn'nnn_
Cuts at: 162 1024 1502 1955 3284 3494 162
Size: 862 478 453 1329 210 1931
Fragments arranged by size:
1931 1329 862 478 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2193 3469 3781 3927 5073 2193
Size: 1276 312 146 1146 2383
Fragments arranged by size:
2383 1276 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 758 1067 1161 1544 1772 2098 3086 3518
Size: 397 309 94 383 228 326 988 432
Cuts at: 3518 4267 4535 361
Size: 749 268 1089
Fragments arranged by size:
1089 988 749 432 397 383 326 309
268 228 94
EcoNI CCTnn'n_nnAGG
Cuts at: 890 890
Size: 5263
EcoO109I rG'GnC_Cy
Cuts at: 236 237 583 1590 2400 236
Size: 1 346 1007 810 3099
Fragments arranged by size:
3099 1007 810 346 1
EcoRI G'AATT_C
Cuts at: 2265 2265
Size: 5263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1495 1984 3280 1495
Size: 489 1296 3478
Fragments arranged by size:
3478 1296 489
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5263
HaeII r_GCGC'y
Cuts at: 546 2695 2943 3967 4946 546
Size: 2149 248 1024 979 863
Fragments arranged by size:
2149 1024 979 863 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 1234 1266 1899 1931 3103 3133 3135
Size: 32 570 32 633 32 1172 30 2
Cuts at: 3135 3165 632
Size: 30 2730
Fragments arranged by size:
2730 1172 633 570 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1118 1784 2300 1118
Size: 666 516 4081
Fragments arranged by size:
4081 666 516
HindIII A'AGCT_T
Cuts at: 690 2110 690
Size: 1420 3843
KasI G'GCGC_C
Cuts at: 2691 2939 2691
Size: 248 5015
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5263
MfeI C'AATT_G
Cuts at: 1900 1900
Size: 5263
MluI A'CGCG_T
Cuts at: 4696 4696
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 3934 4118 1115
Size: 2819 184 2260
Fragments arranged by size:
2819 2260 184
MscI TGG'CCA
Cuts at: 3022 3022
Size: 5263
MslI CAynn'nnrTG
Cuts at: 1075 3250 3377 1075
Size: 2175 127 2961
Fragments arranged by size:
2961 2175 127
MspA1I CmG'CkG
Cuts at: 163 206 300 309 361 452 580 1133 2372
Size: 43 94 9 52 91 128 553 1239
Cuts at: 2372 2686 3046 4061 4306 163
Size: 314 360 1015 245 1120
Fragments arranged by size:
1239 1120 1015 553 360 314 245 128
94 91 52 43 9
NaeI GCC'GGC
Cuts at: 196 2191 3442 196
Size: 1995 1251 2017
Fragments arranged by size:
2017 1995 1251
NarI GG'CG_CC
Cuts at: 2692 2940 2692
Size: 248 5015
NcoI C'CATG_G
Cuts at: 125 125
Size: 5263
NgoMIV G'CCGG_C
Cuts at: 194 2189 3440 194
Size: 1995 1251 2017
Fragments arranged by size:
2017 1995 1251
NruI TCG'CGA
Cuts at: 3624 3624
Size: 5263
NspI r_CATG'y
Cuts at: 2163 2314 2814 3723 2163
Size: 151 500 909 3703
Fragments arranged by size:
3703 909 500 151
PciI A'CATG_T
Cuts at: 3719 3719
Size: 5263
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4402 4434 4402
Size: 32 5231
PpuMI rG'GwC_Cy
Cuts at: 1590 1590
Size: 5263
PspOMI G'GGCC_C
Cuts at: 236 423 464 476 583 236
Size: 187 41 12 107 4916
Fragments arranged by size:
4916 187 107 41 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 809 841 928 960 809
Size: 32 87 32 5112
Fragments arranged by size:
5112 87 32 32
PstI C_TGCA'G
Cuts at: 138 293 2150 2308 2993 138
Size: 155 1857 158 685 2408
Fragments arranged by size:
2408 1857 685 158 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5263
PvuII CAG'CTG
Cuts at: 2686 3046 2686
Size: 360 4903
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5263
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5263
SacII CC_GC'GG
Cuts at: 164 310 453 581 164
Size: 146 143 128 4846
Fragments arranged by size:
4846 146 143 128
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5263
SapI GCTCTTCn'nnn_
Cuts at: 1024 1955 3284 3494 1024
Size: 931 1329 210 2793
Fragments arranged by size:
2793 1329 931 210
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5263
ScaI AGT'ACT
Cuts at: 147 827 4780 147
Size: 680 3953 630
Fragments arranged by size:
3953 680 630
SfcI C'TryA_G
Cuts at: 33 134 289 1002 2146 2304 2989 3984 4175
Size: 101 155 713 1144 158 685 995 191
Cuts at: 4175 33
Size: 1121
Fragments arranged by size:
1144 1121 995 713 685 191 158 155
101
SfiI GGCCn_nnn'nGGCC
Cuts at: 200 200
Size: 5263
SfoI GGC'GCC
Cuts at: 2693 2941 2693
Size: 248 5015
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5263
SmaI CCC'GGG
Cuts at: 428 2283 428
Size: 1855 3408
SmlI C'TyrA_G
Cuts at: 693 890 2416 3331 3825 4087 693
Size: 197 1526 915 494 262 1869
Fragments arranged by size:
1869 1526 915 494 262 197
SnaBI TAC'GTA
Cuts at: 2253 2253
Size: 5263
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5263
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4763
StyI C'CwwG_G
Cuts at: 125 980 1064 1193 2395 2749 125
Size: 855 84 129 1202 354 2639
Fragments arranged by size:
2639 1202 855 354 129 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1864 2147 2844 2977 3436 3647 1864
Size: 283 697 133 459 211 3480
Fragments arranged by size:
3480 697 459 283 211 133
TatI w'GTAC_w
Cuts at: 145 825 1294 4778 145
Size: 680 469 3484 630
Fragments arranged by size:
3484 680 630 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4780 4812 4780
Size: 32 5231
Tth111I GACn'n_nGTC
Cuts at: 2086 3058 2086
Size: 972 4291
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2061 2756 2061
Size: 695 4568
XhoI C'TCGA_G
Cuts at: 890 890
Size: 5263
XmaI C'CCGG_G
Cuts at: 426 2281 426
Size: 1855 3408
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstEII Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI NdeI NheI NotI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI SanDI SexAI
SgrAI SrfI SspI StuI SwaI XmnI ZraI