Restriction map for pF1KSDA1991
 (Linear) (Six-base) MAPSORT of: KIAA1991.seq  Check: 3870  from: 1  to: 2124



KIAA1991.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AccI GT'mk_AC

Cuts at:      0    1044    1477    2124 
   Size:       1044     433     647

  Fragments arranged by size: 

                1044     647     433
 
AclI AA'CG_TT

Cuts at:      0     720    2124 
   Size:        720    1404
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     632    1035    1418    1972    2124 
   Size:        632     403     383     554     152

  Fragments arranged by size: 

                 632     554     403     383     152
 
AfeI AGC'GCT

Cuts at:      0     418    2124 
   Size:        418    1706
 
ApaI G_GGCC'C

Cuts at:      0     114     301     342     354     461    2124 
   Size:        114     187      41      12     107    1663

  Fragments arranged by size: 

                1663     187     114     107      41      12
 
AvaI C'yCGr_G

Cuts at:      0      23     300     412     764    2124 
   Size:         23     277     112     352    1360

  Fragments arranged by size: 

                1360     352     277     112      23
 
BamHI G'GATC_C

Cuts at:      0    1286    2124 
   Size:       1286     838
 
BanI G'GyrC_C

Cuts at:      0     209    2124 
   Size:        209    1915
 
BanII G_rGCy'C

Cuts at:      0     114     221     301     342     354     461    2124 
   Size:        114     107      80      41      12     107    1663

  Fragments arranged by size: 

                1663     114     107     107      80      41      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1786    2009    2124 
   Size:       1786     223     115

  Fragments arranged by size: 

                1786     223     115
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     636    1414    2124 
   Size:        636     778     710

  Fragments arranged by size: 

                 778     710     636
 
BclI T'GATC_A

Cuts at:      0    1503    2124 
   Size:       1503     621
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74     183     292    2124 
   Size:         74     109     109    1832

  Fragments arranged by size: 

                1832     109     109      74
 
BglII A'GATC_T

Cuts at:      0    1307    2124 
   Size:       1307     817
 
Bme1580I G_kGCm'C

Cuts at:      0     114     301     342     354     461    2124 
   Size:        114     187      41      12     107    1663

  Fragments arranged by size: 

                1663     187     114     107      41      12
 
BmgBI CAC'GTC

Cuts at:      0     733    2124 
   Size:        733    1391
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116    1954    2124 
   Size:        116    1838     170

  Fragments arranged by size: 

                1838     170     116
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     941    1646    2124 
   Size:        235     706     705     478

  Fragments arranged by size: 

                 706     705     478     235
 
Bpu10I CC'TnA_GC

Cuts at:      0     825    2124 
   Size:        825    1299
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    2124 
   Size:        588    1536
 
BsaBI GATnn'nnATC

Cuts at:      0    1502    2087    2124 
   Size:       1502     585      37

  Fragments arranged by size: 

                1502     585      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     805     835     939     969    1108    1138    2124 
   Size:        805      30     104      30     139      30     986

  Fragments arranged by size: 

                 986     805     139     104      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1163    2124 
   Size:       1163     961
 
BseYI C'CCAG_C

Cuts at:      0     335     384    2124 
   Size:        335      49    1740

  Fragments arranged by size: 

                1740     335      49
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    2124 
   Size:        713    1411
 
BsiEI CG_ry'CG

Cuts at:      0     187    2124 
   Size:        187    1937
 
BsiHKAI G_wGCw'C

Cuts at:      0     519    2124 
   Size:        519    1605
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      82     739    1017    2124 
   Size:         82     657     278    1107

  Fragments arranged by size: 

                1107     657     278      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     221     301     342     354     461     519    2124 
   Size:        114     107      80      41      12     107      58    1605

  Fragments arranged by size: 

                1605     114     107     107      80      58      41      12
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       1    1452    1591    2124 
   Size:          1    1451     139     533

  Fragments arranged by size: 

                1451     533     139       1
 
BsrBI CCG'CTC

Cuts at:      0      52    1320    2124 
   Size:         52    1268     804

  Fragments arranged by size: 

                1268     804      52
 
BsrDI GCAATG_nn'

Cuts at:      0    1119    2124 
   Size:       1119    1005
 
BsrFI r'CCGG_y

Cuts at:      0      68    2063    2124 
   Size:         68    1995      61

  Fragments arranged by size: 

                1995      68      61
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2109    2124 
   Size:       2109      15
 
BstYI r'GATC_y

Cuts at:      0    1286    1307    1568    2124 
   Size:       1286      21     261     556

  Fragments arranged by size: 

                1286     556     261      21
 
BstZ17I GTA'TAC

Cuts at:      0    1478    2124 
   Size:       1478     646
 
BtgI C'CryG_G

Cuts at:      0      35     181     324     452    1282    2124 
   Size:         35     146     143     128     830     842

  Fragments arranged by size: 

                 842     830     146     143     128      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     772    2124 
   Size:        772    1352
 
BtsI GCAGTG_nn'

Cuts at:      0    1620    1872    2124 
   Size:       1620     252     252

  Fragments arranged by size: 

                1620     252     252
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     241     276    2124 
   Size:        241      35    1848

  Fragments arranged by size: 

                1848     241      35
 
EaeI y'GGCC_r

Cuts at:      0     184     249     363    1194    2111    2124 
   Size:        184      65     114     831     917      13

  Fragments arranged by size: 

                 917     831     184     114      65      13
 
EagI C'GGCC_G

Cuts at:      0     184    2124 
   Size:        184    1940
 
EarI CTCTTCn'nnn_

Cuts at:      0      36     898    1376    1829    2124 
   Size:         36     862     478     453     295

  Fragments arranged by size: 

                 862     478     453     295      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2067    2124 
   Size:       2067      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     632     941    1035    1418    1646    1972    2124 
   Size:        235     397     309      94     383     228     326     152

  Fragments arranged by size: 

                 397     383     326     309     235     228     152      94
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     764    2124 
   Size:        764    1360
 
EcoO109I rG'GnC_Cy

Cuts at:      0     110     111     457    1464    2124 
   Size:        110       1     346    1007     660

  Fragments arranged by size: 

                1007     660     346     110       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1369    1858    2124 
   Size:       1369     489     266

  Fragments arranged by size: 

                1369     489     266
 
HaeII r_GCGC'y

Cuts at:      0     420    2124 
   Size:        420    1704
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     506     538    1108    1140    1773    1805    2124 
   Size:        506      32     570      32     633      32     319

  Fragments arranged by size: 

                 633     570     506     319      32      32      32
 
HincII GTy'rAC

Cuts at:      0     992    1658    2124 
   Size:        992     666     466

  Fragments arranged by size: 

                 992     666     466
 
HindIII A'AGCT_T

Cuts at:      0     564    1984    2124 
   Size:        564    1420     140

  Fragments arranged by size: 

                1420     564     140
 
MfeI C'AATT_G

Cuts at:      0    1774    2124 
   Size:       1774     350
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     989    2124 
   Size:        989    1135
 
MslI CAynn'nnrTG

Cuts at:      0     949    2124 
   Size:        949    1175
 
MspA1I CmG'CkG

Cuts at:      0      37      80     174     183     235     326     454    1007 
   Size:         37      43      94       9      52      91     128     553

Cuts at:   1007    2124 
   Size:       1117

  Fragments arranged by size: 

                1117     553     128      94      91      52      43      37
                   9
 
NaeI GCC'GGC

Cuts at:      0      70    2065    2124 
   Size:         70    1995      59

  Fragments arranged by size: 

                1995      70      59
 
NgoMIV G'CCGG_C

Cuts at:      0      68    2063    2124 
   Size:         68    1995      61

  Fragments arranged by size: 

                1995      68      61
 
NspI r_CATG'y

Cuts at:      0    2037    2124 
   Size:       2037      87
 
PpuMI rG'GwC_Cy

Cuts at:      0    1464    2124 
   Size:       1464     660
 
PspOMI G'GGCC_C

Cuts at:      0     110     297     338     350     457    2124 
   Size:        110     187      41      12     107    1667

  Fragments arranged by size: 

                1667     187     110     107      41      12
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     683     715     802     834    2124 
   Size:        683      32      87      32    1290

  Fragments arranged by size: 

                1290     683      87      32      32
 
PstI C_TGCA'G

Cuts at:      0      12     167    2024    2124 
   Size:         12     155    1857     100

  Fragments arranged by size: 

                1857     155     100      12
 
SacII CC_GC'GG

Cuts at:      0      38     184     327     455    2124 
   Size:         38     146     143     128    1669

  Fragments arranged by size: 

                1669     146     143     128      38
 
SapI GCTCTTCn'nnn_

Cuts at:      0     898    1829    2124 
   Size:        898     931     295

  Fragments arranged by size: 

                 931     898     295
 
ScaI AGT'ACT

Cuts at:      0      21     701    2124 
   Size:         21     680    1423

  Fragments arranged by size: 

                1423     680      21
 
SfcI C'TryA_G

Cuts at:      0       8     163     876    2020    2124 
   Size:          8     155     713    1144     104

  Fragments arranged by size: 

                1144     713     155     104       8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      74    2124 
   Size:         74    2050
 
SmaI CCC'GGG

Cuts at:      0     302    2124 
   Size:        302    1822
 
SmlI C'TyrA_G

Cuts at:      0     567     764    2124 
   Size:        567     197    1360

  Fragments arranged by size: 

                1360     567     197
 
StyI C'CwwG_G

Cuts at:      0     854     938    1067    2124 
   Size:        854      84     129    1057

  Fragments arranged by size: 

                1057     854     129      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1738    2021    2124 
   Size:       1738     283     103

  Fragments arranged by size: 

                1738     283     103
 
TatI w'GTAC_w

Cuts at:      0      19     699    1168    2124 
   Size:         19     680     469     956

  Fragments arranged by size: 

                 956     680     469      19
 
Tth111I GACn'n_nGTC

Cuts at:      0    1960    2124 
   Size:       1960     164
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1935    2124 
   Size:       1935     189
 
XhoI C'TCGA_G

Cuts at:      0     764    2124 
   Size:        764    1360
 
XmaI C'CCGG_G

Cuts at:      0     300    2124 
   Size:        300    1824
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI     ApaI     AvaI    BamHI     BanI
    BanII     BbsI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I
     NaeI   NgoMIV     NspI    PpuMI   PspOMI     PsrI     PstI    SacII
     SapI     ScaI     SfcI     SfiI     SmaI     SmlI     StyI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BfrBI     BlpI     BmtI
     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsiWI     BsmI    BspEI
    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I
     ClaI     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     MscI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SbfI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XmnI     ZraI
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