Restriction map for pF1KSDA1990
 (Linear) (Six-base) MAPSORT of: KIAA1990.seq  Check: 1586  from: 1  to: 2637



KIAA1990.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AatII G_ACGT'C

Cuts at:      0    1850    2637 
   Size:       1850     787
 
AccI GT'mk_AC

Cuts at:      0    2066    2637 
   Size:       2066     571
 
AclI AA'CG_TT

Cuts at:      0    1232    1254    2637 
   Size:       1232      22    1383

  Fragments arranged by size: 

                1383    1232      22
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439    1351    1556    2637 
   Size:        439     912     205    1081

  Fragments arranged by size: 

                1081     912     439     205
 
AflII C'TTAA_G

Cuts at:      0    1804    2637 
   Size:       1804     833
 
AflIII A'CryG_T

Cuts at:      0    1574    2637 
   Size:       1574    1063
 
AgeI A'CCGG_T

Cuts at:      0      11    1357    2637 
   Size:         11    1346    1280

  Fragments arranged by size: 

                1346    1280      11
 
AleI CACnn'nnGTG

Cuts at:      0     166    2637 
   Size:        166    2471
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1303    2637 
   Size:       1303    1334
 
AlwNI CAG_nnn'CTG

Cuts at:      0     251    2637 
   Size:        251    2386
 
ApaLI G'TGCA_C

Cuts at:      0     511    2449    2637 
   Size:        511    1938     188

  Fragments arranged by size: 

                1938     511     188
 
ApoI r'AATT_y

Cuts at:      0     869    2637 
   Size:        869    1768
 
AvaI C'yCGr_G

Cuts at:      0     541    1886    2216    2637 
   Size:        541    1345     330     421

  Fragments arranged by size: 

                1345     541     421     330
 
BamHI G'GATC_C

Cuts at:      0     350    1988    2637 
   Size:        350    1638     649

  Fragments arranged by size: 

                1638     649     350
 
BanII G_rGCy'C

Cuts at:      0    1266    2363    2637 
   Size:       1266    1097     274

  Fragments arranged by size: 

                1266    1097     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     551     986    2637 
   Size:         39     512     435    1651

  Fragments arranged by size: 

                1651     512     435      39
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1099    1133    2637 
   Size:       1099      34    1504

  Fragments arranged by size: 

                1504    1099      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     463     781    2530    2637 
   Size:        463     318    1749     107

  Fragments arranged by size: 

                1749     463     318     107
 
BfrBI ATG'CAT

Cuts at:      0     534    2637 
   Size:        534    2103
 
BglII A'GATC_T

Cuts at:      0     614    2637 
   Size:        614    2023
 
Bme1580I G_kGCm'C

Cuts at:      0     163     515    2453    2637 
   Size:        163     352    1938     184

  Fragments arranged by size: 

                1938     352     184     163
 
BmgBI CAC'GTC

Cuts at:      0      86     570    2637 
   Size:         86     484    2067

  Fragments arranged by size: 

                2067     484      86
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     993    1619    2637 
   Size:        993     626    1018

  Fragments arranged by size: 

                1018     993     626
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     486    2637 
   Size:        486    2151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689     979    1066    1252    1861    1916    2443    2637 
   Size:        689     290      87     186     609      55     527     194

  Fragments arranged by size: 

                 689     609     527     290     194     186      87      55
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     265    2637 
   Size:        265    2372
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     670     814    1069    2637 
   Size:        670     144     255    1568

  Fragments arranged by size: 

                1568     670     255     144
 
BsaBI GATnn'nnATC

Cuts at:      0    2527    2637 
   Size:       2527     110
 
BsaHI Gr'CG_yC

Cuts at:      0    1847    2258    2637 
   Size:       1847     411     379

  Fragments arranged by size: 

                1847     411     379
 
BsaWI w'CCGG_w

Cuts at:      0      11     623    1357    2105    2369    2637 
   Size:         11     612     734     748     264     268

  Fragments arranged by size: 

                 748     734     612     268     264      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1630    1660    2637 
   Size:       1630      30     977

  Fragments arranged by size: 

                1630     977      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493     772    1035    1193    1370    2637 
   Size:        493     279     263     158     177    1267

  Fragments arranged by size: 

                1267     493     279     263     177     158
 
BseYI C'CCAG_C

Cuts at:      0     741    1343    2113    2637 
   Size:        741     602     770     524

  Fragments arranged by size: 

                 770     741     602     524
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1144    2637 
   Size:       1144    1493
 
BsiEI CG_ry'CG

Cuts at:      0    1392    2637 
   Size:       1392    1245
 
BsiHKAI G_wGCw'C

Cuts at:      0     271     515    2453    2637 
   Size:        271     244    1938     184

  Fragments arranged by size: 

                1938     271     244     184
 
BsmI GAATG_Cn'

Cuts at:      0     703    2115    2637 
   Size:        703    1412     522

  Fragments arranged by size: 

                1412     703     522
 
Bsp1286I G_dGCh'C

Cuts at:      0     163     271     515    1266    2363    2453    2637 
   Size:        163     108     244     751    1097      90     184

  Fragments arranged by size: 

                1097     751     244     184     163     108      90
 
BspEI T'CCGG_A

Cuts at:      0    2105    2637 
   Size:       2105     532
 
BspHI T'CATG_A

Cuts at:      0    1052    1760    2637 
   Size:       1052     708     877

  Fragments arranged by size: 

                1052     877     708
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1773    1841    2637 
   Size:       1773      68     796

  Fragments arranged by size: 

                1773     796      68
 
BsrFI r'CCGG_y

Cuts at:      0      11     762    1357    1391    2637 
   Size:         11     751     595      34    1246

  Fragments arranged by size: 

                1246     751     595      34      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     592    2637 
   Size:        592    2045
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     483    1419    1656    2196    2637 
   Size:        483     936     237     540     441

  Fragments arranged by size: 

                 936     540     483     441     237
 
BstYI r'GATC_y

Cuts at:      0     350     614    1988    2637 
   Size:        350     264    1374     649

  Fragments arranged by size: 

                1374     649     350     264
 
BstZ17I GTA'TAC

Cuts at:      0    2067    2637 
   Size:       2067     570
 
BtgI C'CryG_G

Cuts at:      0     107    2637 
   Size:        107    2530
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2514    2637 
   Size:       2514     123
 
BtsI GCAGTG_nn'

Cuts at:      0     269     557    1127    2409    2637 
   Size:        269     288     570    1282     228

  Fragments arranged by size: 

                1282     570     288     269     228
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     833     868    1686    1721    2637 
   Size:        833      35     818      35     916

  Fragments arranged by size: 

                 916     833     818      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1680    2637 
   Size:       1680     957
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1783    2637 
   Size:       1783     854
 
EaeI y'GGCC_r

Cuts at:      0     485     723    2088    2637 
   Size:        485     238    1365     549

  Fragments arranged by size: 

                1365     549     485     238
 
EarI CTCTTCn'nnn_

Cuts at:      0    1648    2132    2471    2587    2637 
   Size:       1648     484     339     116      50

  Fragments arranged by size: 

                1648     484     339     116      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     689     979    1066    1252    1351    1556    1861 
   Size:        439     250     290      87     186      99     205     305

Cuts at:   1861    1916    2443    2637 
   Size:         55     527     194

  Fragments arranged by size: 

                 527     439     305     290     250     205     194     186
                  99      87      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1470    2637 
   Size:       1470    1167
 
HaeII r_GCGC'y

Cuts at:      0    2430    2598    2637 
   Size:       2430     168      39

  Fragments arranged by size: 

                2430     168      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169     613     645     954     986    1311    1343 
   Size:        137      32     444      32     309      32     325      32

Cuts at:   1343    1564    1588    1596    1620    1869    1888    1901    1920 
   Size:        221      24       8      24     249      19      13      19

Cuts at:   1920    2018    2050    2637 
   Size:         98      32     587

  Fragments arranged by size: 

                 587     444     325     309     249     221     137      98
                  32      32      32      32      32      24      24      19
                  19      13       8
 
HincII GTy'rAC

Cuts at:      0     714    2637 
   Size:        714    1923
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     776    1795    2637 
   Size:          9     767    1019     842

  Fragments arranged by size: 

                1019     842     767       9
 
MscI TGG'CCA

Cuts at:      0     725    2090    2637 
   Size:        725    1365     547

  Fragments arranged by size: 

                1365     725     547
 
MslI CAynn'nnrTG

Cuts at:      0     166    1573    2637 
   Size:        166    1407    1064

  Fragments arranged by size: 

                1407    1064     166
 
MspA1I CmG'CkG

Cuts at:      0      91     565     957    1343    2637 
   Size:         91     474     392     386    1294

  Fragments arranged by size: 

                1294     474     392     386      91
 
NcoI C'CATG_G

Cuts at:      0     107    2637 
   Size:        107    2530
 
NruI TCG'CGA

Cuts at:      0    2255    2637 
   Size:       2255     382
 
NsiI A_TGCA'T

Cuts at:      0     536    2637 
   Size:        536    2101
 
NspI r_CATG'y

Cuts at:      0     538     784    1578    1695    1818    2065    2637 
   Size:        538     246     794     117     123     247     572

  Fragments arranged by size: 

                 794     572     538     247     246     123     117
 
PciI A'CATG_T

Cuts at:      0    1574    2637 
   Size:       1574    1063
 
PfoI T'CCnGG_A

Cuts at:      0    1838    2312    2637 
   Size:       1838     474     325

  Fragments arranged by size: 

                1838     474     325
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84    2637 
   Size:         52      32    2553

  Fragments arranged by size: 

                2553      52      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1470    2637 
   Size:       1470    1167
 
PshAI GACnn'nnGTC

Cuts at:      0    2211    2637 
   Size:       2211     426
 
PspXI vC'TCGA_Gb

Cuts at:      0    1886    2637 
   Size:       1886     751
 
PstI C_TGCA'G

Cuts at:      0     833    1122    1729    2270    2637 
   Size:        833     289     607     541     367

  Fragments arranged by size: 

                 833     607     541     367     289
 
PvuII CAG'CTG

Cuts at:      0      91     957    2637 
   Size:         91     866    1680

  Fragments arranged by size: 

                1680     866      91
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2132    2471    2637 
   Size:       2132     339     166

  Fragments arranged by size: 

                2132     339     166
 
ScaI AGT'ACT

Cuts at:      0    1450    2637 
   Size:       1450    1187
 
SexAI A'CCwGG_T

Cuts at:      0     244     518    2637 
   Size:        244     274    2119

  Fragments arranged by size: 

                2119     274     244
 
SfcI C'TryA_G

Cuts at:      0     829     973    1118    1633    1725    2266    2409    2415 
   Size:        829     144     145     515      92     541     143       6

Cuts at:   2415    2637 
   Size:        222

  Fragments arranged by size: 

                 829     541     515     222     145     144     143      92
                   6
 
SmlI C'TyrA_G

Cuts at:      0    1804    1886    2637 
   Size:       1804      82     751

  Fragments arranged by size: 

                1804     751      82
 
SphI G_CATG'C

Cuts at:      0    1695    2637 
   Size:       1695     942
 
StyI C'CwwG_G

Cuts at:      0     107     815    2347    2552    2637 
   Size:        107     708    1532     205      85

  Fragments arranged by size: 

                1532     708     205     107      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     136     419     694    2602    2637 
   Size:        136     283     275    1908      35

  Fragments arranged by size: 

                1908     283     275     136      35
 
TatI w'GTAC_w

Cuts at:      0    1448    1859    2637 
   Size:       1448     411     778

  Fragments arranged by size: 

                1448     778     411
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1546    1578    2637 
   Size:       1546      32    1059

  Fragments arranged by size: 

                1546    1059      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2310    2637 
   Size:       2310     327
 
XhoI C'TCGA_G

Cuts at:      0    1886    2637 
   Size:       1886     751
 
ZraI GAC'GTC

Cuts at:      0    1848    2637 
   Size:       1848     789
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AvaI    BamHI    BanII     BbsI
     BcgI    BciVI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrFI   BstAPI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI EcoO109I
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     NruI     NsiI     NspI     PciI     PfoI     PpiI    PpuMI    PshAI
    PspXI     PstI    PvuII     SapI     ScaI    SexAI     SfcI     SmlI
     SphI     StyI    TaqII     TatI     TstI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI     AhdI     AloI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BclI     BglI
     BlpI     BmtI    BpuEI    BsaAI    BsiWI    BsmBI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII   Bsu36I     ClaI     DraI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     PacI     PasI
    PflMI     PmeI     PmlI     PsiI   PspOMI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XmaI     XmnI
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