Restriction map for
pF1KSDA1969
(Linear) (Six-base) MAPSORT of: KIAA1969.seq Check: 2646 from: 1 to: 1728
KIAA1969.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AgeI A'CCGG_T
Cuts at: 0 893 1313 1728
Size: 893 420 415
Fragments arranged by size:
893 420 415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 63 1728
Size: 63 1665
ApaLI G'TGCA_C
Cuts at: 0 466 1728
Size: 466 1262
ApoI r'AATT_y
Cuts at: 0 127 171 420 559 671 687 755 1175
Size: 127 44 249 139 112 16 68 420
Cuts at: 1175 1427 1595 1728
Size: 252 168 133
Fragments arranged by size:
420 252 249 168 139 133 127 112
68 44 16
BanII G_rGCy'C
Cuts at: 0 278 1728
Size: 278 1450
BbsI GAAGACnn'nnnn_
Cuts at: 0 207 974 1728
Size: 207 767 754
Fragments arranged by size:
767 754 207
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 1728
Size: 35 1693
BlpI GC'TnA_GC
Cuts at: 0 164 1728
Size: 164 1564
Bme1580I G_kGCm'C
Cuts at: 0 470 1728
Size: 470 1258
BmrI ACTGGGnnnn_n'
Cuts at: 0 241 857 1728
Size: 241 616 871
Fragments arranged by size:
871 616 241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1712 1728
Size: 1712 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 280 785 953 1037 1121 1205 1289
Size: 157 123 505 168 84 84 84 84
Cuts at: 1289 1373 1457 1707 1728
Size: 84 84 250 21
Fragments arranged by size:
505 250 168 157 123 84 84 84
84 84 84 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1728
Size: 16 1712
BsaWI w'CCGG_w
Cuts at: 0 893 1313 1728
Size: 893 420 415
Fragments arranged by size:
893 420 415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1728
Size: 154 1574
BseYI C'CCAG_C
Cuts at: 0 315 1728
Size: 315 1413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1728
Size: 1316 412
BsiHKAI G_wGCw'C
Cuts at: 0 470 1728
Size: 470 1258
BsmI GAATG_Cn'
Cuts at: 0 154 1728
Size: 154 1574
Bsp1286I G_dGCh'C
Cuts at: 0 278 470 1728
Size: 278 192 1258
Fragments arranged by size:
1258 278 192
BsrFI r'CCGG_y
Cuts at: 0 893 1313 1728
Size: 893 420 415
Fragments arranged by size:
893 420 415
ClaI AT'CG_AT
Cuts at: 0 1062 1728
Size: 1062 666
DraI TTT'AAA
Cuts at: 0 566 723 1728
Size: 566 157 1005
Fragments arranged by size:
1005 566 157
EaeI y'GGCC_r
Cuts at: 0 119 349 1728
Size: 119 230 1379
Fragments arranged by size:
1379 230 119
EarI CTCTTCn'nnn_
Cuts at: 0 283 1728
Size: 283 1445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 280 785 953 1037 1121 1205 1289
Size: 157 123 505 168 84 84 84 84
Cuts at: 1289 1373 1457 1707 1728
Size: 84 84 250 21
Fragments arranged by size:
505 250 168 157 123 84 84 84
84 84 84 21
EcoRI G'AATT_C
Cuts at: 0 127 671 687 755 1175 1427 1728
Size: 127 544 16 68 420 252 301
Fragments arranged by size:
544 420 301 252 127 68 16
HincII GTy'rAC
Cuts at: 0 661 1728
Size: 661 1067
HindIII A'AGCT_T
Cuts at: 0 716 800 968 1052 1304 1388 1472 1728
Size: 716 84 168 84 252 84 84 256
Fragments arranged by size:
716 256 252 168 84 84 84 84
MscI TGG'CCA
Cuts at: 0 121 351 1728
Size: 121 230 1377
Fragments arranged by size:
1377 230 121
MslI CAynn'nnrTG
Cuts at: 0 864 1728
Size: 864 864
NspI r_CATG'y
Cuts at: 0 645 1728
Size: 645 1083
PasI CC'CwG_GG
Cuts at: 0 52 1728
Size: 52 1676
PsiI TTA'TAA
Cuts at: 0 482 1728
Size: 482 1246
SexAI A'CCwGG_T
Cuts at: 0 209 1728
Size: 209 1519
SmlI C'TyrA_G
Cuts at: 0 31 1728
Size: 31 1697
SphI G_CATG'C
Cuts at: 0 645 1728
Size: 645 1083
SspI AAT'ATT
Cuts at: 0 524 1728
Size: 524 1204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 1728
Size: 211 1517
TatI w'GTAC_w
Cuts at: 0 994 1728
Size: 994 734
Tth111I GACn'n_nGTC
Cuts at: 0 247 1728
Size: 247 1481
XmnI GAAnn'nnTTC
Cuts at: 0 607 775 1728
Size: 607 168 953
Fragments arranged by size:
953 607 168
Enzymes that do cut:
AgeI AlfI ApaLI ApoI BanII BbsI BciVI BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BsrFI ClaI DraI EaeI
EarI Eco57MI EcoRI HincII HindIII MscI MslI NspI
PasI PsiI SexAI SmlI SphI SspI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BmgBI BmtI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
Hin4I HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI StyI SwaI TstI XbaI XcmI XhoI
XmaI ZraI