Restriction map for pF1KSDA1963
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BseRI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeIII      MamI        MfeI        MluI        
MroI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          145  GGCGCC               G[GA]^CG[CT]C
AflIII        308  ACACGT               A^C[GA][CT]GT
AvaI          451  CCCGGG               C^[CT]CG[GA]G
AvaII         185  GGACC                G^G[AT]CC
BalI          302  TGGCCA               TGG^CCA
BanII         577  GAGCTC               G[GA]GC[CT]^C
BbeI          145  GGCGCC               GGCGC^C
BbrPI         421  CACGTG               CAC^GTG
BbsI          749  GTCTTC               GAAGAC(2/6)
BfaI          922  CTAG                 C^TAG
BglI          137  GCCGCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         749  GTCTTC               GAAGAC(2/6)
BsaAI         421  CACGTG               [CT]AC^GT[GA]
BsaHI         145  GGCGCC               G[GA]^CG[CT]C
BsaI          609  GGTCTC               GGTCTC(1/5)
BsiEI         208  CGGCCG               CG[GA][CT]^CG
BsmFI         184  GGGAC                GGGAC(10/14)
BsoBI         451  CCCGGG               C^[CT]CG[GA]G
BspMI         416  ACCTGC               ACCTGC(4/8)
BsrFI         694  ACCGGC               [GA]^CCGG[CT]
BssSI         889  CTCGTG               CACGAG(-5/-1)
BstAPI        733  GCAGGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        809  GGATG                GGATG(2/0)
BstXI         722  CCATCGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        694  ACCGGC               [GA]^CCGG[CT]
DraII         184  GGGACCC              [GA]G^G[ACGT]CC[CT]
EagI          208  CGGCCG               C^GGCCG
EclXI         208  CGGCCG               C^GGCCG
Eco52I        208  CGGCCG               C^GGCCG
EcoNI         342  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      184  GGGACCC              [GA]G^G[ACGT]CC[CT]
FokI          809  GGATG                GGATG(9/13)
Hin1I         145  GGCGCC               G[GA]^CG[CT]C
KasI          145  GGCGCC               G^GCGCC
MaeI          922  CTAG                 C^TAG
MluNI         302  TGGCCA               TGG^CCA
MscI          302  TGGCCA               TGG^CCA
NarI          145  GGCGCC               GG^CGCC
PleI          152  GACTC                GAGTC(4/5)
PmaCI         421  CACGTG               CAC^GTG
PmlI          421  CACGTG               CAC^GTG
PpuMI         184  GGGACCC              [GA]G^G[AT]CC[CT]
SacI          577  GAGCTC               GAGCT^C
SfoI          145  GGCGCC               GGC^GCC
SmaI          451  CCCGGG               CCC^GGG
StuI          712  AGGCCT               AGG^CCT
TfiI          817  GAATC                G^A[AT]TC
XmaCI         451  CCCGGG               C^CCGGG
XmaI          451  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          690  GTAC                 GT^AC
              939  GTAC                
AspHI         538  GTGCTC               G[AT]GC[AT]^C
              577  GAGCTC              
BanI          145  GGCGCC               G^G[CT][GA]CC
              509  GGCACC              
BpmI          574  CTGGAG               CTGGAG(16/14)
              699  CTGGAG              
BsiHKAI       538  GTGCTC               G[AT]GC[AT]^C
              577  GAGCTC              
BsmAI         204  GTCTC                GTCTC(1/5)
              610  GTCTC               
BsrBI          97  CCGCTC               CCGCTC(-3/-3)
              157  CCGCTC              
DdeI          470  CTGAG                C^T[ACGT]AG
              881  CTAAG               
DsaI          138  CCGCGG               C^C[GA][CT]GG
              433  CCGCGG              
EaeI          208  CGGCCG               [CT]^GGCC[GA]
              302  TGGCCA              
EarI          541  CTCTTC               CTCTTC(1/4)
              580  CTCTTC              
HgaI          352  GACGC                GACGC(5/10)
              789  GCGTC               
HinfI         152  GACTC                G^A[ACGT]TC
              817  GAATC               
Ksp632I       541  CTCTTC               CTCTTC(1/4)
              580  CTCTTC              
KspI          138  CCGCGG               CCGC^GG
              433  CCGCGG              
MseI          785  TTAA                 T^TAA
              917  TTAA                
MslI           57  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              723  CATCGACATG          
NlaIII         63  CATG                 CATG^
              729  CATG                
PvuII         232  CAGCTG               CAG^CTG
              328  CAGCTG              
RsaI          690  GTAC                 GT^AC
              939  GTAC                
SacII         138  CCGCGG               CCGC^GG
              433  CCGCGG              
SapI          540  GCTCTTC              GCTCTTC(1/4)
              579  GCTCTTC             
SfaNI         591  GATGC                GCATC(5/9)
              810  GATGC               
SfcI          264  CTACAG               C^T[GA][CT]AG
              567  CTATAG              
Tru9I         785  TTAA                 T^TAA
              917  TTAA                
Tsp509I        51  AATT                 ^AATT
              960  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BamHI       
BbvI        BcnI        BmyI        BsaJI       BsgI        
BsiYI       BslI        Bsp1286I    BsrI        BssHII      
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeII       
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TaqI        TseI        TspRI       TthHB8I     XhoII       
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