Restriction map for pF1KSDA1951
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# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BbeI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KasI        KpnI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1087  GTAGAC               GT^[AC][GT]AC
AcyI         1409  GGCGTC               G[GA]^CG[CT]C
AocI          625  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1604  AGCGCT               AGC^GCT
AvaII        1779  GGTCC                G^G[AT]CC
AviII        1460  TGCGCA               TGC^GCA
BalI         1535  TGGCCA               TGG^CCA
BbrPI        1456  CACGTG               CAC^GTG
BbsI         1018  GTCTTC               GAAGAC(2/6)
BcgI         1161  GCATCGCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          667  CTAG                 C^TAG
BlpI          505  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      505  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1018  GTCTTC               GAAGAC(2/6)
BsaAI        1456  CACGTG               [CT]AC^GT[GA]
BsaBI        1820  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1409  GGCGTC               G[GA]^CG[CT]C
BsmI          334  GAATGC               GAATGC(1/-1)
Bsp1407I     1000  TGTACA               T^GTACA
BsrGI        1000  TGTACA               T^GTACA
BstYI         410  AGATCC               [GA]^GATC[CT]
Bsu36I        625  CCTGAGG              CC^T[ACGT]AGG
CelII         505  GCTGAGC              GC^T[ACGT]AGC
Eco47III     1604  AGCGCT               AGC^GCT
Eco81I        625  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1246  CCTCCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1460  TGCGCA               TGC^GCA
Hin1I        1409  GGCGTC               G[GA]^CG[CT]C
KspI         1098  CCGCGG               CCGC^GG
MaeI          667  CTAG                 C^TAG
MamI         1820  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          410  AGATCC               [GA]^GATC[CT]
MluNI        1535  TGGCCA               TGG^CCA
MscI         1535  TGGCCA               TGG^CCA
NspI          236  ACATGC               [GA]CATG^[CT]
PmaCI        1456  CACGTG               CAC^GTG
PmlI         1456  CACGTG               CAC^GTG
SacII        1098  CCGCGG               CCGC^GG
SspBI        1000  TGTACA               T^GTACA
TaqI          474  TCGA                 T^CGA
TthHB8I       474  TCGA                 T^CGA
XhoII         410  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI         1001  GTAC                 GT^AC
             1390  GTAC                
Alw44I       1571  GTGCAC               G^TGCAC
             1872  GTGCAC              
AlwI          411  GATCC                GGATC(4/5)
             1452  GATCC               
AlwNI         436  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1915  CAGCCACTG           
ApaLI        1571  GTGCAC               G^TGCAC
             1872  GTGCAC              
AvaI          454  CCCGAG               C^[CT]CG[GA]G
             1787  CCCGAG              
BsaI         1888  GAGACC               GGTCTC(1/5)
             1696  GGTCTC              
BsgI         1955  CTGCAC               GTGCAG(16/14)
             1385  GTGCAG              
BsoBI         454  CCCGAG               C^[CT]CG[GA]G
             1787  CCCGAG              
BsrDI        1157  CATTGC               GCAATG(2/0)
              683  GCAATG              
BstAPI        231  GCAGCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1044  GCAGCACTTGC         
BstXI         978  CCATGTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1506  CCACTCATGTGG        
HaeII        1604  AGCGCT               [GA]GCGC^[CT]
             1923  GGCGCT              
HgaI         1803  GACGC                GACGC(5/10)
             1410  GCGTC               
HincII         66  GTCAAC               GT[CT]^[GA]AC
               99  GTCAAC              
HindII         66  GTCAAC               GT[CT]^[GA]AC
               99  GTCAAC              
NaeI          863  GCCGGC               GCC^GGC
             1228  GCCGGC              
NcoI          758  CCATGG               C^CATGG
             1751  CCATGG              
NgoMIV        863  GCCGGC               G^CCGGC
             1228  GCCGGC              
PflMI        1584  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1914  CCAGCCACTGG         
RsaI         1001  GTAC                 GT^AC
             1390  GTAC                
SmlI         1039  CTTGAG               C^T[CT][GA]AG
             1927  CTTGAG              
Van91I       1584  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1914  CCAGCCACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AspHI       
BanI        BanII       BbvCI       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SgrAI       StyI        TseI        Tsp45I      
TspRI       
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