Restriction map for pF1KSDA1951
 (Linear) (Six-base) MAPSORT of: KIAA1951.seq  Check: 7785  from: 1  to: 2010



KIAA1951.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1323    1478    2010 
   Size:       1323     155     532

  Fragments arranged by size: 

                1323     532     155
 
AccI GT'mk_AC

Cuts at:      0    1088    2010 
   Size:       1088     922
 
AfeI AGC'GCT

Cuts at:      0    1606    2010 
   Size:       1606     404
 
AleI CACnn'nnGTG

Cuts at:      0     996    1123    1382    1418    1484    1511    2010 
   Size:        996     127     259      36      66      27     499

  Fragments arranged by size: 

                 996     499     259     127      66      36      27
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     257     509    1400    1486    2010 
   Size:        257     252     891      86     524

  Fragments arranged by size: 

                 891     524     257     252      86
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441    1920    2010 
   Size:        441    1479      90

  Fragments arranged by size: 

                1479     441      90
 
ApaI G_GGCC'C

Cuts at:      0     320     978    1143    1400    2010 
   Size:        320     658     165     257     610

  Fragments arranged by size: 

                 658     610     320     257     165
 
ApaLI G'TGCA_C

Cuts at:      0    1571    1872    2010 
   Size:       1571     301     138

  Fragments arranged by size: 

                1571     301     138
 
AvaI C'yCGr_G

Cuts at:      0     454    1787    2010 
   Size:        454    1333     223

  Fragments arranged by size: 

                1333     454     223
 
BanI G'GyrC_C

Cuts at:      0     300     649    1242    1376    2010 
   Size:        300     349     593     134     634

  Fragments arranged by size: 

                 634     593     349     300     134
 
BanII G_rGCy'C

Cuts at:      0     320     368     587     816     861     978    1143    1286 
   Size:        320      48     219     229      45     117     165     143

Cuts at:   1286    1400    1621    1794    1985    2010 
   Size:        114     221     173     191      25

  Fragments arranged by size: 

                 320     229     221     219     191     173     165     143
                 117     114      48      45      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1011    2010 
   Size:       1011     999
 
BbvCI CC'TCA_GC

Cuts at:      0      17     311     849    1356    1543    2010 
   Size:         17     294     538     507     187     467

  Fragments arranged by size: 

                 538     507     467     294     187      17
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1150    1184    2010 
   Size:       1150      34     826

  Fragments arranged by size: 

                1150     826      34
 
BlpI GC'TnA_GC

Cuts at:      0     506    2010 
   Size:        506    1504
 
Bme1580I G_kGCm'C

Cuts at:      0     320     652     978    1143    1245    1400    1575    1876 
   Size:        320     332     326     165     102     155     175     301

Cuts at:   1876    2010 
   Size:        134

  Fragments arranged by size: 

                 332     326     320     301     175     165     155     134
                 102
 
BmgBI CAC'GTC

Cuts at:      0    1576    2010 
   Size:       1576     434
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1969    2010 
   Size:       1969      41
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     307    1259    1832    2010 
   Size:        226      81     952     573     178

  Fragments arranged by size: 

                 952     573     226     178      81
 
Bpu10I CC'TnA_GC

Cuts at:      0      17     311     815     849    1356    1543    2010 
   Size:         17     294     504      34     507     187     467

  Fragments arranged by size: 

                 507     504     467     294     187      34      17
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    1948    2010 
   Size:       1060     888      62

  Fragments arranged by size: 

                1060     888      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1702    1882    2010 
   Size:       1702     180     128

  Fragments arranged by size: 

                1702     180     128
 
BsaAI yAC'GTr

Cuts at:      0    1458    2010 
   Size:       1458     552
 
BsaBI GATnn'nnATC

Cuts at:      0    1824    2010 
   Size:       1824     186
 
BsaHI Gr'CG_yC

Cuts at:      0    1410    2010 
   Size:       1410     600
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     646     676    2010 
   Size:        646      30    1334

  Fragments arranged by size: 

                1334     646      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     268     271     643     691     709     712     730     766 
   Size:        268       3     372      48      18       3      18      36

Cuts at:    766    2010 
   Size:       1244

  Fragments arranged by size: 

                1244     372     268      48      36      18      18       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     425     482     809     836    1269    1319    1881    1913 
   Size:        425      57     327      27     433      50     562      32

Cuts at:   1913    2010 
   Size:         97

  Fragments arranged by size: 

                 562     433     425     327      97      57      50      32
                  27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1406    1940    2010 
   Size:       1406     534      70

  Fragments arranged by size: 

                1406     534      70
 
BsiHKAI G_wGCw'C

Cuts at:      0     178     368     607    1342    1575    1621    1794    1876 
   Size:        178     190     239     735     233      46     173      82

Cuts at:   1876    2010 
   Size:        134

  Fragments arranged by size: 

                 735     239     233     190     178     173     134      82
                  46
 
BsmI GAATG_Cn'

Cuts at:      0     340    2010 
   Size:        340    1670
 
Bsp1286I G_dGCh'C

Cuts at:      0     178     320     368     587     607     652     816     861 
   Size:        178     142      48     219      20      45     164      45

Cuts at:    861     978    1143    1245    1286    1342    1400    1575    1621 
   Size:        117     165     102      41      56      58     175      46

Cuts at:   1621    1794    1876    1985    2010 
   Size:        173      82     109      25

  Fragments arranged by size: 

                 219     178     175     173     165     164     142     117
                 109     102      82      58      56      48      46      45
                  45      41      25      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1015    1323    1478    1634    2010 
   Size:       1015     308     155     156     376

  Fragments arranged by size: 

                1015     376     308     156     155
 
BsrDI GCAATG_nn'

Cuts at:      0     690    1156    2010 
   Size:        690     466     854

  Fragments arranged by size: 

                 854     690     466
 
BsrFI r'CCGG_y

Cuts at:      0     863     872     953    1133    1214    1228    1379    1637 
   Size:        863       9      81     180      81      14     151     258

Cuts at:   1637    2010 
   Size:        373

  Fragments arranged by size: 

                 863     373     258     180     151      81      81      14
                   9
 
BsrGI T'GTAC_A

Cuts at:      0    1000    2010 
   Size:       1000    1010
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     237    1050    2010 
   Size:        237     813     960

  Fragments arranged by size: 

                 960     813     237
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     985    1513    2010 
   Size:        985     528     497

  Fragments arranged by size: 

                 985     528     497
 
BstYI r'GATC_y

Cuts at:      0     410    2010 
   Size:        410    1600
 
Bsu36I CC'TnA_GG

Cuts at:      0     626    2010 
   Size:        626    1384
 
BtgI C'CryG_G

Cuts at:      0     758    1098    1751    2010 
   Size:        758     340     653     259

  Fragments arranged by size: 

                 758     653     340     259
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1019    1045    1147    1878    2010 
   Size:       1019      26     102     731     132

  Fragments arranged by size: 

                1019     731     132     102      26
 
BtsI GCAGTG_nn'

Cuts at:      0      62     544     915    1145    2010 
   Size:         62     482     371     230     865

  Fragments arranged by size: 

                 865     482     371     230      62
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     509     544    2010 
   Size:        509      35    1466

  Fragments arranged by size: 

                1466     509      35
 
EaeI y'GGCC_r

Cuts at:      0      23    1095    1535    1654    1734    2010 
   Size:         23    1072     440     119      80     276

  Fragments arranged by size: 

                1072     440     276     119      80      23
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     698     731    2010 
   Size:        154     544      33    1279

  Fragments arranged by size: 

                1279     544     154      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     891     936    2010 
   Size:        891      45    1074

  Fragments arranged by size: 

                1074     891      45
 
EcoICRI GAG'CTC

Cuts at:      0     366    1619    1792    2010 
   Size:        366    1253     173     218

  Fragments arranged by size: 

                1253     366     218     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     307    1259    1832    2010 
   Size:        226      81     952     573     178

  Fragments arranged by size: 

                 952     573     226     178      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1250    2010 
   Size:       1250     760
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     316     317    1397    1493    2010 
   Size:        132     184       1    1080      96     517

  Fragments arranged by size: 

                1080     517     184     132      96       1
 
FspI TGC'GCA

Cuts at:      0    1462    2010 
   Size:       1462     548
 
HaeII r_GCGC'y

Cuts at:      0    1608    1927    2010 
   Size:       1608     319      83

  Fragments arranged by size: 

                1608     319      83
 
HincII GTy'rAC

Cuts at:      0      68     101    2010 
   Size:         68      33    1909

  Fragments arranged by size: 

                1909      68      33
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    1646    1973    2010 
   Size:        671     975     327      37

  Fragments arranged by size: 

                 975     671     327      37
 
MscI TGG'CCA

Cuts at:      0    1537    2010 
   Size:       1537     473
 
MslI CAynn'nnrTG

Cuts at:      0     380     983     996    1123    1382    1418    1484    1511 
   Size:        380     603      13     127     259      36      66      27

Cuts at:   1511    1568    2010 
   Size:         57     442

  Fragments arranged by size: 

                 603     442     380     259     127      66      57      36
                  27      13
 
MspA1I CmG'CkG

Cuts at:      0     805    1100    1279    1323    1418    1557    1739    1776 
   Size:        805     295     179      44      95     139     182      37

Cuts at:   1776    1944    2010 
   Size:        168      66

  Fragments arranged by size: 

                 805     295     182     179     168     139      95      66
                  44      37
 
NaeI GCC'GGC

Cuts at:      0     865    1230    2010 
   Size:        865     365     780

  Fragments arranged by size: 

                 865     780     365
 
NcoI C'CATG_G

Cuts at:      0     758    1751    2010 
   Size:        758     993     259

  Fragments arranged by size: 

                 993     758     259
 
NgoMIV G'CCGG_C

Cuts at:      0     863    1228    2010 
   Size:        863     365     782

  Fragments arranged by size: 

                 863     782     365
 
NspI r_CATG'y

Cuts at:      0     240    2010 
   Size:        240    1770
 
PasI CC'CwG_GG

Cuts at:      0     358    2010 
   Size:        358    1652
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1590    1920    2010 
   Size:       1590     330      90

  Fragments arranged by size: 

                1590     330      90
 
PmlI CAC'GTG

Cuts at:      0    1458    2010 
   Size:       1458     552
 
PspOMI G'GGCC_C

Cuts at:      0     316     974    1139    1396    2010 
   Size:        316     658     165     257     614

  Fragments arranged by size: 

                 658     614     316     257     165
 
PstI C_TGCA'G

Cuts at:      0     922     962    1631    1943    2010 
   Size:        922      40     669     312      67

  Fragments arranged by size: 

                 922     669     312      67      40
 
PvuII CAG'CTG

Cuts at:      0     805    1323    1557    1944    2010 
   Size:        805     518     234     387      66

  Fragments arranged by size: 

                 805     518     387     234      66
 
SacI G_AGCT'C

Cuts at:      0     368    1621    1794    2010 
   Size:        368    1253     173     216

  Fragments arranged by size: 

                1253     368     216     173
 
SacII CC_GC'GG

Cuts at:      0    1101    2010 
   Size:       1101     909
 
SfcI C'TryA_G

Cuts at:      0     918     958    1627    1807    1939    2010 
   Size:        918      40     669     180     132      71

  Fragments arranged by size: 

                 918     669     180     132      71      40
 
SgrAI Cr'CCGG_yG

Cuts at:      0     872    1133    1214    1379    1637    2010 
   Size:        872     261      81     165     258     373

  Fragments arranged by size: 

                 872     373     261     258     165      81
 
SmlI C'TyrA_G

Cuts at:      0    1039    1927    2010 
   Size:       1039     888      83

  Fragments arranged by size: 

                1039     888      83
 
StyI C'CwwG_G

Cuts at:      0     758    1013    1751    2010 
   Size:        758     255     738     259

  Fragments arranged by size: 

                 758     738     259     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     453     667     919    2010 
   Size:        453     214     252    1091

  Fragments arranged by size: 

                1091     453     252     214
 
TatI w'GTAC_w

Cuts at:      0    1000    1389    2010 
   Size:       1000     389     621

  Fragments arranged by size: 

                1000     621     389
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     646     678    2010 
   Size:        646      32    1332

  Fragments arranged by size: 

                1332     646      32
 

 Enzymes that do cut:

     AarI     AccI     AfeI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII   HincII     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI
     PmlI   PspOMI     PstI    PvuII     SacI    SacII     SfcI    SgrAI
     SmlI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BciVI     BclI    BfrBI     BglI    BglII     BmtI     BplI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI    BstBI   BstEII  BstZ17I     ClaI     DraI   DraIII     DrdI
     EagI    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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