(Circular) (Six-base) MAPSORT of: pF1KSDA1916.seq Check: 3230 from: 1 to: 4772 pF1KSDA1916 4772 bp With 182 enzymes: SgfI * June 20, 2008 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 201 201 Size: 4772 AccI GT'mk_AC Cuts at: 1810 1810 Size: 4772 Acc65I G'GTAC_C Cuts at: 1788 1788 Size: 4772 AclI AA'CG_TT Cuts at: 4612 4612 Size: 4772 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 567 2597 3029 3776 4044 567 Size: 2030 432 747 268 1295 Fragments arranged by size: 2030 1295 747 432 268 AfeI AGC'GCT Cuts at: 155 155 Size: 4772 AflII C'TTAA_G Cuts at: 1752 1752 Size: 4772 AflIII A'CryG_T Cuts at: 2124 3228 4205 2124 Size: 1104 977 2691 Fragments arranged by size: 2691 1104 977 AgeI A'CCGG_T Cuts at: 3160 3160 Size: 4772 AlwNI CAG_nnn'CTG Cuts at: 1224 2077 3644 1224 Size: 853 1567 2352 Fragments arranged by size: 2352 1567 853 ApaLI G'TGCA_C Cuts at: 3542 3542 Size: 4772 ApoI r'AATT_y Cuts at: 402 628 681 918 1057 1066 1776 4125 4381 Size: 226 53 237 139 9 710 2349 256 Cuts at: 4381 402 Size: 793 Fragments arranged by size: 2349 793 710 256 237 226 139 53 9 AseI AT'TA_AT Cuts at: 20 20 Size: 4772 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4772 AvaI C'yCGr_G Cuts at: 157 1454 1508 1792 157 Size: 1297 54 284 3137 Fragments arranged by size: 3137 1297 284 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 706 739 706 Size: 33 4739 BamHI G'GATC_C Cuts at: 1797 1797 Size: 4772 BanI G'GyrC_C Cuts at: 181 288 1650 1788 2202 2450 2485 181 Size: 107 1362 138 414 248 35 2468 Fragments arranged by size: 2468 1362 414 248 138 107 35 BanII G_rGCy'C Cuts at: 280 1786 2816 280 Size: 1506 1030 2236 Fragments arranged by size: 2236 1506 1030 BbeI G_GCGC'C Cuts at: 185 1654 2206 2454 185 Size: 1469 552 248 2503 Fragments arranged by size: 2503 1469 552 248 BbsI GAAGACnn'nnnn_ Cuts at: 573 573 Size: 4772 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1636 1670 1636 Size: 34 4738 BciVI GTATCCnnnnn_n' Cuts at: 819 2670 3431 4772 819 Size: 1851 761 1341 819 Fragments arranged by size: 1851 1341 819 761 BclI T'GATC_A Cuts at: 984 2292 984 Size: 1308 3464 BglII A'GATC_T Cuts at: 2287 4324 2287 Size: 2037 2735 BlpI GC'TnA_GC Cuts at: 1884 1884 Size: 4772 Bme1580I G_kGCm'C Cuts at: 293 2397 2490 3546 293 Size: 2104 93 1056 1519 Fragments arranged by size: 2104 1519 1056 93 BmgBI CAC'GTC Cuts at: 747 747 Size: 4772 BmrI ACTGGGnnnn_n' Cuts at: 1984 2065 2400 4544 1984 Size: 81 335 2144 2212 Fragments arranged by size: 2212 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3139 3139 Size: 4772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 916 1306 916 Size: 390 4382 Bpu10I CC'TnA_GC Cuts at: 322 322 Size: 4772 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1948 2827 3319 3617 1948 Size: 879 492 298 3103 Fragments arranged by size: 3103 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1089 4476 1089 Size: 3387 1385 BsaAI yAC'GTr Cuts at: 855 1764 2125 855 Size: 909 361 3502 Fragments arranged by size: 3502 909 361 BsaBI GATnn'nnATC Cuts at: 6 2286 4323 6 Size: 2280 2037 455 Fragments arranged by size: 2280 2037 455 BsaHI Gr'CG_yC Cuts at: 182 1651 2203 2451 3153 4649 182 Size: 1469 552 248 702 1496 305 Fragments arranged by size: 1496 1469 702 552 305 248 BsaWI w'CCGG_w Cuts at: 923 1961 2482 3160 3434 3581 3992 923 Size: 1038 521 678 274 147 411 1703 Fragments arranged by size: 1703 1038 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 301 331 301 Size: 30 4742 BseYI C'CCAG_C Cuts at: 177 871 1007 2197 2955 3532 4028 177 Size: 694 136 1190 758 577 496 921 Fragments arranged by size: 1190 921 758 694 577 496 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1210 1666 1210 Size: 456 4316 BsiEI CG_ry'CG Cuts at: 104 2314 2360 3568 104 Size: 2210 46 1208 1308 Fragments arranged by size: 2210 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1786 2564 2754 3546 1786 Size: 778 190 792 3012 Fragments arranged by size: 3012 792 778 190 BsmI GAATG_Cn' Cuts at: 772 1412 772 Size: 640 4132 BsmBI CGTCTCn'nnnn_ Cuts at: 1129 1129 Size: 4772 Bsp1286I G_dGCh'C Cuts at: 280 293 1786 2397 2490 2564 2754 2816 3546 Size: 13 1493 611 93 74 190 62 730 Cuts at: 3546 280 Size: 1506 Fragments arranged by size: 1506 1493 730 611 190 93 74 62 13 BspEI T'CCGG_A Cuts at: 923 3992 923 Size: 3069 1703 BspHI T'CATG_A Cuts at: 1460 3948 1460 Size: 2488 2284 BspMI ACCTGCnnnn'nnnn_ Cuts at: 201 1822 2036 2338 2719 201 Size: 1621 214 302 381 2254 Fragments arranged by size: 2254 1621 381 302 214 BsrBI CCG'CTC Cuts at: 50 1301 3064 3118 4602 4767 50 Size: 1251 1763 54 1484 165 55 Fragments arranged by size: 1763 1484 1251 165 55 54 BsrDI GCAATG_nn' Cuts at: 2684 2684 Size: 4772 BsrFI r'CCGG_y Cuts at: 2770 2951 3160 2770 Size: 181 209 4382 Fragments arranged by size: 4382 209 181 BssHII G'CGCG_C Cuts at: 206 226 206 Size: 20 4752 BssSI C'ACGA_G Cuts at: 1228 3043 3401 1228 Size: 1815 358 2599 Fragments arranged by size: 2599 1815 358 BstAPI GCAn_nnn'nTGC Cuts at: 2149 2149 Size: 4772 BstBI TT'CG_AA Cuts at: 110 3133 110 Size: 3023 1749 BstYI r'GATC_y Cuts at: 1 1490 1797 2264 2287 2622 2868 3880 3966 Size: 1489 307 467 23 335 246 1012 86 Cuts at: 3966 3978 4324 1 Size: 12 346 449 Fragments arranged by size: 1489 1012 467 449 346 335 307 246 86 23 12 BtgI C'CryG_G Cuts at: 125 199 1356 125 Size: 74 1157 3541 Fragments arranged by size: 3541 1157 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 846 1134 2722 2903 3066 846 Size: 288 1588 181 163 2552 Fragments arranged by size: 2552 1588 288 181 163 BtsI GCAGTG_nn' Cuts at: 1222 2015 1222 Size: 793 3979 ClaI AT'CG_AT Cuts at: 980 1268 980 Size: 288 4484 DraI TTT'AAA Cuts at: 1542 1771 1542 Size: 229 4543 DrdI GACnn_nn'nnGTC Cuts at: 1081 1456 2478 3336 1081 Size: 375 1022 858 2517 Fragments arranged by size: 2517 1022 858 375 EaeI y'GGCC_r Cuts at: 545 857 2357 2531 2922 2949 4294 545 Size: 312 1500 174 391 27 1345 1023 Fragments arranged by size: 1500 1345 1023 391 312 174 27 EagI C'GGCC_G Cuts at: 2357 2357 Size: 4772 EarI CTCTTCn'nnn_ Cuts at: 285 1018 1390 1505 2795 3005 285 Size: 733 372 115 1290 210 2052 Fragments arranged by size: 2052 1290 733 372 210 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2980 3290 3436 4582 2980 Size: 310 146 1146 3170 Fragments arranged by size: 3170 1146 310 146 EcoICRI GAG'CTC Cuts at: 1784 1784 Size: 4772 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 567 916 1306 2597 3029 3776 4044 567 Size: 349 390 1291 432 747 268 1295 Fragments arranged by size: 1295 1291 747 432 390 349 268 EcoO109I rG'GnC_Cy Cuts at: 1308 1911 1308 Size: 603 4169 EcoRI G'AATT_C Cuts at: 402 1776 402 Size: 1374 3398 EcoRV GAT'ATC Cuts at: 1266 1266 Size: 4772 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 2791 Size: 4772 FspI TGC'GCA Cuts at: 2553 2553 Size: 4772 HaeII r_GCGC'y Cuts at: 133 157 185 1654 2206 2454 3476 4455 133 Size: 24 28 1469 552 248 1022 979 450 Fragments arranged by size: 1469 1022 979 552 450 248 28 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 301 333 580 612 1261 1293 1482 1514 2614 Size: 32 247 32 649 32 189 32 1100 Cuts at: 2614 2644 2646 2676 301 Size: 30 2 30 2397 Fragments arranged by size: 2397 1100 649 247 189 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1749 1811 1749 Size: 62 4710 KasI G'GCGC_C Cuts at: 181 1650 2202 2450 181 Size: 1469 552 248 2503 Fragments arranged by size: 2503 1469 552 248 KpnI G_GTAC'C Cuts at: 1792 1792 Size: 4772 MluI A'CGCG_T Cuts at: 4205 4205 Size: 4772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1030 3443 3627 1030 Size: 2413 184 2175 Fragments arranged by size: 2413 2175 184 MscI TGG'CCA Cuts at: 547 859 2533 547 Size: 312 1674 2786 Fragments arranged by size: 2786 1674 312 MslI CAynn'nnrTG Cuts at: 271 913 2761 2888 271 Size: 642 1848 127 2155 Fragments arranged by size: 2155 1848 642 127 MspA1I CmG'CkG Cuts at: 201 248 390 1669 1883 2197 2557 3570 3815 Size: 47 142 1279 214 314 360 1013 245 Cuts at: 3815 201 Size: 1158 Fragments arranged by size: 1279 1158 1013 360 314 245 214 142 47 NaeI GCC'GGC Cuts at: 2953 2953 Size: 4772 NarI GG'CG_CC Cuts at: 182 1651 2203 2451 182 Size: 1469 552 248 2503 Fragments arranged by size: 2503 1469 552 248 NcoI C'CATG_G Cuts at: 125 1356 125 Size: 1231 3541 NgoMIV G'CCGG_C Cuts at: 2951 2951 Size: 4772 NspI r_CATG'y Cuts at: 1825 2325 3232 1825 Size: 500 907 3365 Fragments arranged by size: 3365 907 500 PasI CC'CwG_GG Cuts at: 1563 1563 Size: 4772 PciI A'CATG_T Cuts at: 3228 3228 Size: 4772 PfoI T'CCnGG_A Cuts at: 425 425 Size: 4772 PmeI GTTT'AAAC Cuts at: 1771 1771 Size: 4772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 3911 Size: 32 4740 PpuMI rG'GwC_Cy Cuts at: 1308 1308 Size: 4772 PsiI TTA'TAA Cuts at: 1187 1187 Size: 4772 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 835 867 835 Size: 32 4740 PstI C_TGCA'G Cuts at: 389 1529 1819 2504 389 Size: 1140 290 685 2657 Fragments arranged by size: 2657 1140 685 290 PvuI CG_AT'CG Cuts at: 104 104 Size: 4772 PvuII CAG'CTG Cuts at: 248 390 1669 2197 2557 248 Size: 142 1279 528 360 2463 Fragments arranged by size: 2463 1279 528 360 142 RsrII CG'GwC_CG Cuts at: 2967 2967 Size: 4772 SacI G_AGCT'C Cuts at: 1786 1786 Size: 4772 SacII CC_GC'GG Cuts at: 202 202 Size: 4772 SalI G'TCGA_C Cuts at: 1809 1809 Size: 4772 SapI GCTCTTCn'nnn_ Cuts at: 285 1018 1390 1505 2795 3005 285 Size: 733 372 115 1290 210 2052 Fragments arranged by size: 2052 1290 733 372 210 115 SbfI CC_TGCA'GG Cuts at: 1819 1819 Size: 4772 ScaI AGT'ACT Cuts at: 4289 4289 Size: 4772 SfcI C'TryA_G Cuts at: 33 385 776 1525 1815 2500 3493 3684 33 Size: 352 391 749 290 685 993 191 1121 Fragments arranged by size: 1121 993 749 685 391 352 290 191 SfoI GGC'GCC Cuts at: 183 1652 2204 2452 183 Size: 1469 552 248 2503 Fragments arranged by size: 2503 1469 552 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4772 SmaI CCC'GGG Cuts at: 1456 1510 1794 1456 Size: 54 284 4434 Fragments arranged by size: 4434 284 54 SmlI C'TyrA_G Cuts at: 1752 1927 2842 3334 3596 1752 Size: 175 915 492 262 2928 Fragments arranged by size: 2928 915 492 262 175 SnaBI TAC'GTA Cuts at: 1764 1764 Size: 4772 SpeI A'CTAG_T Cuts at: 67 67 Size: 4772 SphI G_CATG'C Cuts at: 1825 2325 1825 Size: 500 4272 StyI C'CwwG_G Cuts at: 125 254 571 1356 1906 2260 125 Size: 129 317 785 550 354 2637 Fragments arranged by size: 2637 785 550 354 317 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 276 374 2355 2488 2947 3156 276 Size: 98 1981 133 459 209 1892 Fragments arranged by size: 1981 1892 459 209 133 98 TatI w'GTAC_w Cuts at: 4287 4287 Size: 4772 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4289 4321 4289 Size: 32 4740 Tth111I GACn'n_nGTC Cuts at: 2569 2569 Size: 4772 XbaI T'CTAG_A Cuts at: 1803 1803 Size: 4772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1015 2267 1015 Size: 1252 3520 XmaI C'CCGG_G Cuts at: 1454 1508 1792 1454 Size: 54 284 4434 Fragments arranged by size: 4434 284 54 XmnI GAAnn'nnTTC Cuts at: 366 1399 366 Size: 1033 3739 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AhdI AleI AlfI AloI ApaI AscI AvrII BbvCI BfrBI BglI BmtI BseRI BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PspOMI PspXI SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI