Restriction map for pF1KSDA1916
 (Linear) (Six-base) MAPSORT of: KIAA1916.seq  Check: 5497  from: 1  to: 1635



KIAA1916.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      75    1635 
   Size:         75    1560
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441    1635 
   Size:        441    1194
 
AfeI AGC'GCT

Cuts at:      0      29    1635 
   Size:         29    1606
 
AflII C'TTAA_G

Cuts at:      0    1626    1635 
   Size:       1626       9
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1098    1635 
   Size:       1098     537
 
ApoI r'AATT_y

Cuts at:      0     276     502     555     792     931     940    1635 
   Size:        276     226      53     237     139       9     695

  Fragments arranged by size: 

                 695     276     237     226     139      53       9
 
AvaI C'yCGr_G

Cuts at:      0      31    1328    1382    1635 
   Size:         31    1297      54     253

  Fragments arranged by size: 

                1297     253      54      31
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     580     613    1635 
   Size:        580      33    1022

  Fragments arranged by size: 

                1022     580      33
 
BanI G'GyrC_C

Cuts at:      0      55     162    1524    1635 
   Size:         55     107    1362     111

  Fragments arranged by size: 

                1362     111     107      55
 
BanII G_rGCy'C

Cuts at:      0     154    1635 
   Size:        154    1481
 
BbeI G_GCGC'C

Cuts at:      0      59    1528    1635 
   Size:         59    1469     107

  Fragments arranged by size: 

                1469     107      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     447    1635 
   Size:        447    1188
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1510    1544    1635 
   Size:       1510      34      91

  Fragments arranged by size: 

                1510      91      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     693    1635 
   Size:        693     942
 
BclI T'GATC_A

Cuts at:      0     858    1635 
   Size:        858     777
 
Bme1580I G_kGCm'C

Cuts at:      0     167    1635 
   Size:        167    1468
 
BmgBI CAC'GTC

Cuts at:      0     621    1635 
   Size:        621    1014
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1180    1635 
   Size:        790     390     455

  Fragments arranged by size: 

                 790     455     390
 
Bpu10I CC'TnA_GC

Cuts at:      0     196    1635 
   Size:        196    1439
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     963    1635 
   Size:        963     672
 
BsaAI yAC'GTr

Cuts at:      0     729    1635 
   Size:        729     906
 
BsaHI Gr'CG_yC

Cuts at:      0      56    1525    1635 
   Size:         56    1469     110

  Fragments arranged by size: 

                1469     110      56
 
BsaWI w'CCGG_w

Cuts at:      0     797    1635 
   Size:        797     838
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205    1635 
   Size:        175      30    1430

  Fragments arranged by size: 

                1430     175      30
 
BseYI C'CCAG_C

Cuts at:      0      51     745     881    1635 
   Size:         51     694     136     754

  Fragments arranged by size: 

                 754     694     136      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1540    1635 
   Size:       1084     456      95

  Fragments arranged by size: 

                1084     456      95
 
BsmI GAATG_Cn'

Cuts at:      0     646    1286    1635 
   Size:        646     640     349

  Fragments arranged by size: 

                 646     640     349
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1003    1635 
   Size:       1003     632
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     167    1635 
   Size:        154      13    1468

  Fragments arranged by size: 

                1468     154      13
 
BspEI T'CCGG_A

Cuts at:      0     797    1635 
   Size:        797     838
 
BspHI T'CATG_A

Cuts at:      0    1334    1635 
   Size:       1334     301
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      75    1635 
   Size:         75    1560
 
BsrBI CCG'CTC

Cuts at:      0    1175    1635 
   Size:       1175     460
 
BssHII G'CGCG_C

Cuts at:      0      80     100    1635 
   Size:         80      20    1535

  Fragments arranged by size: 

                1535      80      20
 
BssSI C'ACGA_G

Cuts at:      0    1102    1635 
   Size:       1102     533
 
BstYI r'GATC_y

Cuts at:      0    1364    1635 
   Size:       1364     271
 
BtgI C'CryG_G

Cuts at:      0      73    1230    1635 
   Size:         73    1157     405

  Fragments arranged by size: 

                1157     405      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     720    1008    1635 
   Size:        720     288     627

  Fragments arranged by size: 

                 720     627     288
 
BtsI GCAGTG_nn'

Cuts at:      0    1096    1635 
   Size:       1096     539
 
ClaI AT'CG_AT

Cuts at:      0     854    1142    1635 
   Size:        854     288     493

  Fragments arranged by size: 

                 854     493     288
 
DraI TTT'AAA

Cuts at:      0    1416    1635 
   Size:       1416     219
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     955    1330    1635 
   Size:        955     375     305

  Fragments arranged by size: 

                 955     375     305
 
EaeI y'GGCC_r

Cuts at:      0     419     731    1635 
   Size:        419     312     904

  Fragments arranged by size: 

                 904     419     312
 
EarI CTCTTCn'nnn_

Cuts at:      0     159     892    1264    1379    1635 
   Size:        159     733     372     115     256

  Fragments arranged by size: 

                 733     372     256     159     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441     790    1180    1635 
   Size:        441     349     390     455

  Fragments arranged by size: 

                 455     441     390     349
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1182    1635 
   Size:       1182     453
 
EcoRI G'AATT_C

Cuts at:      0     276    1635 
   Size:        276    1359
 
EcoRV GAT'ATC

Cuts at:      0    1140    1635 
   Size:       1140     495
 
HaeII r_GCGC'y

Cuts at:      0       7      31      59    1528    1635 
   Size:          7      24      28    1469     107

  Fragments arranged by size: 

                1469     107      28      24       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207     454     486    1135    1167    1356    1388 
   Size:        175      32     247      32     649      32     189      32

Cuts at:   1388    1635 
   Size:        247

  Fragments arranged by size: 

                 649     247     247     189     175      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1623    1635 
   Size:       1623      12
 
KasI G'GCGC_C

Cuts at:      0      55    1524    1635 
   Size:         55    1469     111

  Fragments arranged by size: 

                1469     111      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1635 
   Size:        904     731
 
MscI TGG'CCA

Cuts at:      0     421     733    1635 
   Size:        421     312     902

  Fragments arranged by size: 

                 902     421     312
 
MslI CAynn'nnrTG

Cuts at:      0     145     787    1635 
   Size:        145     642     848

  Fragments arranged by size: 

                 848     642     145
 
MspA1I CmG'CkG

Cuts at:      0      75     122     264    1543    1635 
   Size:         75      47     142    1279      92

  Fragments arranged by size: 

                1279     142      92      75      47
 
NarI GG'CG_CC

Cuts at:      0      56    1525    1635 
   Size:         56    1469     110

  Fragments arranged by size: 

                1469     110      56
 
NcoI C'CATG_G

Cuts at:      0    1230    1635 
   Size:       1230     405
 
PasI CC'CwG_GG

Cuts at:      0    1437    1635 
   Size:       1437     198
 
PfoI T'CCnGG_A

Cuts at:      0     299    1635 
   Size:        299    1336
 
PpuMI rG'GwC_Cy

Cuts at:      0    1182    1635 
   Size:       1182     453
 
PsiI TTA'TAA

Cuts at:      0    1061    1635 
   Size:       1061     574
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     709     741    1635 
   Size:        709      32     894

  Fragments arranged by size: 

                 894     709      32
 
PstI C_TGCA'G

Cuts at:      0     263    1403    1635 
   Size:        263    1140     232

  Fragments arranged by size: 

                1140     263     232
 
PvuII CAG'CTG

Cuts at:      0     122     264    1543    1635 
   Size:        122     142    1279      92

  Fragments arranged by size: 

                1279     142     122      92
 
SacII CC_GC'GG

Cuts at:      0      76    1635 
   Size:         76    1559
 
SapI GCTCTTCn'nnn_

Cuts at:      0     159     892    1264    1379    1635 
   Size:        159     733     372     115     256

  Fragments arranged by size: 

                 733     372     256     159     115
 
SfcI C'TryA_G

Cuts at:      0     259     650    1399    1635 
   Size:        259     391     749     236

  Fragments arranged by size: 

                 749     391     259     236
 
SfoI GGC'GCC

Cuts at:      0      57    1526    1635 
   Size:         57    1469     109

  Fragments arranged by size: 

                1469     109      57
 
SmaI CCC'GGG

Cuts at:      0    1330    1384    1635 
   Size:       1330      54     251

  Fragments arranged by size: 

                1330     251      54
 
SmlI C'TyrA_G

Cuts at:      0    1626    1635 
   Size:       1626       9
 
StyI C'CwwG_G

Cuts at:      0     128     445    1230    1635 
   Size:        128     317     785     405

  Fragments arranged by size: 

                 785     405     317     128
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     150     248    1635 
   Size:        150      98    1387

  Fragments arranged by size: 

                1387     150      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     889    1635 
   Size:        889     746
 
XmaI C'CCGG_G

Cuts at:      0    1328    1382    1635 
   Size:       1328      54     253

  Fragments arranged by size: 

                1328     253      54
 
XmnI GAAnn'nnTTC

Cuts at:      0     240    1273    1635 
   Size:        240    1033     362

  Fragments arranged by size: 

                1033     362     240
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AflII    AlwNI     ApoI     AvaI     BaeI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI Bme1580I
    BmgBI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI   BssHII    BssSI    BstYI     BtgI    BtgZI     BtsI     ClaI
     DraI     DrdI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     PasI     PfoI    PpuMI     PsiI     PsrI     PstI
    PvuII    SacII     SapI     SfcI     SfoI     SmaI     SmlI     StyI
    TaqII     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI    BbvCI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI
     BplI    BpuEI    BsaBI    BseRI    BsiEI  BsiHKAI    BsiWI    BsrDI
    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    CspCI   DraIII     EagI     EciI  EcoICRI    EcoNI     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PvuI    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com