Restriction map for pF1KSDA1899
 (Linear) (Six-base) MAPSORT of: KIAA1899.seq  Check: 9551  from: 1  to: 3072



KIAA1899.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1313    1744    3072 
   Size:       1313     431    1328

  Fragments arranged by size: 

                1328    1313     431
 
AccI GT'mk_AC

Cuts at:      0     557    3072 
   Size:        557    2515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     515    1082    1687    1726    2333    3072 
   Size:        126     389     567     605      39     607     739

  Fragments arranged by size: 

                 739     607     605     567     389     126      39
 
AflII C'TTAA_G

Cuts at:      0     528    2655    3072 
   Size:        528    2127     417

  Fragments arranged by size: 

                2127     528     417
 
AflIII A'CryG_T

Cuts at:      0    1535    2216    3072 
   Size:       1535     681     856

  Fragments arranged by size: 

                1535     856     681
 
AgeI A'CCGG_T

Cuts at:      0    2816    3072 
   Size:       2816     256
 
AleI CACnn'nnGTG

Cuts at:      0    1642    3072 
   Size:       1642    1430
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1711    1907    3072 
   Size:       1711     196    1165

  Fragments arranged by size: 

                1711    1165     196
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1704    3072 
   Size:       1704    1368
 
ApaI G_GGCC'C

Cuts at:      0    1101    1622    3072 
   Size:       1101     521    1450

  Fragments arranged by size: 

                1450    1101     521
 
ApaLI G'TGCA_C

Cuts at:      0    1441    3072 
   Size:       1441    1631
 
ApoI r'AATT_y

Cuts at:      0     200     211     268     548    2172    2456    2783    3072 
   Size:        200      11      57     280    1624     284     327     289

  Fragments arranged by size: 

                1624     327     289     284     280     200      57      11
            
 
BamHI G'GATC_C

Cuts at:      0     600    3013    3072 
   Size:        600    2413      59

  Fragments arranged by size: 

                2413     600      59
 
BanI G'GyrC_C

Cuts at:      0    1206    1448    1462    3072 
   Size:       1206     242      14    1610

  Fragments arranged by size: 

                1610    1206     242      14
 
BanII G_rGCy'C

Cuts at:      0      65    1101    1622    3072 
   Size:         65    1036     521    1450

  Fragments arranged by size: 

                1450    1036     521      65
 
BbeI G_GCGC'C

Cuts at:      0    1466    3072 
   Size:       1466    1606
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1801    3072 
   Size:       1801    1271
 
BbvCI CC'TCA_GC

Cuts at:      0    2401    3072 
   Size:       2401     671
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1683    1717    1972    2006    2623    2657    3072 
   Size:       1683      34     255      34     617      34     415

  Fragments arranged by size: 

                1683     617     415     255      34      34      34
 
BclI T'GATC_A

Cuts at:      0     516    3072 
   Size:        516    2556
 
BfrBI ATG'CAT

Cuts at:      0    2673    3072 
   Size:       2673     399
 
Bme1580I G_kGCm'C

Cuts at:      0    1101    1445    1451    1622    2920    3072 
   Size:       1101     344       6     171    1298     152

  Fragments arranged by size: 

                1298    1101     344     171     152       6
 
BmgBI CAC'GTC

Cuts at:      0      54    2219    2326    3072 
   Size:         54    2165     107     746

  Fragments arranged by size: 

                2165     746     107      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     284     435     684    3072 
   Size:        284     151     249    2388

  Fragments arranged by size: 

                2388     284     249     151
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2683    3072 
   Size:       2683     389
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     964    1274    1608    2752    3072 
   Size:         70     894     310     334    1144     320

  Fragments arranged by size: 

                1144     894     334     320     310      70
 
Bpu10I CC'TnA_GC

Cuts at:      0    2401    3072 
   Size:       2401     671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1210    3072 
   Size:       1210    1862
 
BsaBI GATnn'nnATC

Cuts at:      0    2768    3072 
   Size:       2768     304
 
BsaHI Gr'CG_yC

Cuts at:      0    1463    3072 
   Size:       1463    1609
 
BsaWI w'CCGG_w

Cuts at:      0    2816    3072 
   Size:       2816     256
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1050    1080    3072 
   Size:       1050      30    1992

  Fragments arranged by size: 

                1992    1050      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2207    3072 
   Size:       2207     865
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1219    3072 
   Size:       1219    1853
 
BsiHKAI G_wGCw'C

Cuts at:      0      65    1445    1732    3072 
   Size:         65    1380     287    1340

  Fragments arranged by size: 

                1380    1340     287      65
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1377    3072 
   Size:       1377    1695
 
Bsp1286I G_dGCh'C

Cuts at:      0      65    1101    1445    1451    1622    1732    2920    3072 
   Size:         65    1036     344       6     171     110    1188     152

  Fragments arranged by size: 

                1188    1036     344     171     152     110      65       6
            
 
BspHI T'CATG_A

Cuts at:      0    1923    2702    2861    3072 
   Size:       1923     779     159     211

  Fragments arranged by size: 

                1923     779     211     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1313    1424    1721    1744    3072 
   Size:       1313     111     297      23    1328

  Fragments arranged by size: 

                1328    1313     297     111      23
 
BsrDI GCAATG_nn'

Cuts at:      0    1889    3072 
   Size:       1889    1183
 
BsrFI r'CCGG_y

Cuts at:      0      79    2816    3072 
   Size:         79    2737     256

  Fragments arranged by size: 

                2737     256      79
 
BsrGI T'GTAC_A

Cuts at:      0     749    1103    1319    1797    3072 
   Size:        749     354     216     478    1275

  Fragments arranged by size: 

                1275     749     478     354     216
 
BssSI C'ACGA_G

Cuts at:      0    1981    3072 
   Size:       1981    1091
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1736    3072 
   Size:       1736    1336
 
BstYI r'GATC_y

Cuts at:      0     600    2062    3013    3072 
   Size:        600    1462     951      59

  Fragments arranged by size: 

                1462     951     600      59
 
BtgI C'CryG_G

Cuts at:      0      19    1640    2416    2423    3072 
   Size:         19    1621     776       7     649

  Fragments arranged by size: 

                1621     776     649      19       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2626    3072 
   Size:       2626     446
 
BtsI GCAGTG_nn'

Cuts at:      0     762    1614    1723    1930    3072 
   Size:        762     852     109     207    1142

  Fragments arranged by size: 

                1142     852     762     207     109
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1175    1210    2476    2511    3072 
   Size:       1175      35    1266      35     561

  Fragments arranged by size: 

                1266    1175     561      35      35
 
DraI TTT'AAA

Cuts at:      0    2961    2993    3072 
   Size:       2961      32      79

  Fragments arranged by size: 

                2961      79      32
 
DraIII CAC_nnn'GTG

Cuts at:      0    1242    3072 
   Size:       1242    1830
 
EaeI y'GGCC_r

Cuts at:      0     960    3072 
   Size:        960    2112
 
EarI CTCTTCn'nnn_

Cuts at:      0     247     500    3072 
   Size:        247     253    2572

  Fragments arranged by size: 

                2572     253     247
 
EcoICRI GAG'CTC

Cuts at:      0      63    3072 
   Size:         63    3009
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     126     515     964    1082    1274    1608    1687 
   Size:         70      56     389     449     118     192     334      79

Cuts at:   1687    1726    2333    2752    3072 
   Size:         39     607     419     320

  Fragments arranged by size: 

                 607     449     419     389     334     320     192     118
                  79      70      56      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1321    1626    3072 
   Size:       1321     305    1446

  Fragments arranged by size: 

                1446    1321     305
 
EcoO109I rG'GnC_Cy

Cuts at:      0     683    1098    1618    1619    2412    3072 
   Size:        683     415     520       1     793     660

  Fragments arranged by size: 

                 793     683     660     520     415       1
 
EcoRI G'AATT_C

Cuts at:      0     548    3072 
   Size:        548    2524
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1150    2491    2590    2859    3072 
   Size:       1150    1341      99     269     213

  Fragments arranged by size: 

                1341    1150     269     213      99
 
HaeII r_GCGC'y

Cuts at:      0    1466    3072 
   Size:       1466    1606
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2005    2037    2054    2086    2822    2854    3072 
   Size:       2005      32      17      32     736      32     218

  Fragments arranged by size: 

                2005     736     218      32      32      32      17
 
HincII GTy'rAC

Cuts at:      0     168    1058    2352    2804    3072 
   Size:        168     890    1294     452     268

  Fragments arranged by size: 

                1294     890     452     268     168
 
KasI G'GCGC_C

Cuts at:      0    1462    3072 
   Size:       1462    1610
 
MfeI C'AATT_G

Cuts at:      0    2773    3072 
   Size:       2773     299
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     161     229     595    1329    2304    3072 
   Size:         11     150      68     366     734     975     768

  Fragments arranged by size: 

                 975     768     734     366     150      68      11
 
MscI TGG'CCA

Cuts at:      0     962    3072 
   Size:        962    2110
 
MslI CAynn'nnrTG

Cuts at:      0    1642    2866    3072 
   Size:       1642    1224     206

  Fragments arranged by size: 

                1642    1224     206
 
MspA1I CmG'CkG

Cuts at:      0    1701    3072 
   Size:       1701    1371
 
NaeI GCC'GGC

Cuts at:      0      81    3072 
   Size:         81    2991
 
NarI GG'CG_CC

Cuts at:      0    1463    3072 
   Size:       1463    1609
 
NcoI C'CATG_G

Cuts at:      0    1640    2416    3072 
   Size:       1640     776     656

  Fragments arranged by size: 

                1640     776     656
 
NdeI CA'TA_TG

Cuts at:      0     957    2972    3072 
   Size:        957    2015     100

  Fragments arranged by size: 

                2015     957     100
 
NgoMIV G'CCGG_C

Cuts at:      0      79    3072 
   Size:         79    2993
 
NsiI A_TGCA'T

Cuts at:      0    2675    3072 
   Size:       2675     397
 
NspI r_CATG'y

Cuts at:      0    1026    2039    2649    3072 
   Size:       1026    1013     610     423

  Fragments arranged by size: 

                1026    1013     610     423
 
PfoI T'CCnGG_A

Cuts at:      0      86     980    3072 
   Size:         86     894    2092

  Fragments arranged by size: 

                2092     894      86
 
PpuMI rG'GwC_Cy

Cuts at:      0    2412    3072 
   Size:       2412     660
 
PshAI GACnn'nnGTC

Cuts at:      0     464    2819    3072 
   Size:        464    2355     253

  Fragments arranged by size: 

                2355     464     253
 
PsiI TTA'TAA

Cuts at:      0    2437    2462    3072 
   Size:       2437      25     610

  Fragments arranged by size: 

                2437     610      25
 
PspOMI G'GGCC_C

Cuts at:      0    1097    1618    3072 
   Size:       1097     521    1454

  Fragments arranged by size: 

                1454    1097     521
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1250    1282    3072 
   Size:       1250      32    1790

  Fragments arranged by size: 

                1790    1250      32
 
PstI C_TGCA'G

Cuts at:      0    1421    1718    2550    3072 
   Size:       1421     297     832     522

  Fragments arranged by size: 

                1421     832     522     297
 
PvuII CAG'CTG

Cuts at:      0    1701    3072 
   Size:       1701    1371
 
SacI G_AGCT'C

Cuts at:      0      65    3072 
   Size:         65    3007
 
ScaI AGT'ACT

Cuts at:      0     670    1251    2544    3072 
   Size:        670     581    1293     528

  Fragments arranged by size: 

                1293     670     581     528
 
SfcI C'TryA_G

Cuts at:      0    1417    1714    2546    2790    2936    3006    3072 
   Size:       1417     297     832     244     146      70      66

  Fragments arranged by size: 

                1417     832     297     244     146      70      66
 
SfoI GGC'GCC

Cuts at:      0    1464    3072 
   Size:       1464    1608
 
SmlI C'TyrA_G

Cuts at:      0     528    1225    2655    3072 
   Size:        528     697    1430     417

  Fragments arranged by size: 

                1430     697     528     417
 
SphI G_CATG'C

Cuts at:      0    1026    3072 
   Size:       1026    2046
 
SspI AAT'ATT

Cuts at:      0     897    1111    3072 
   Size:        897     214    1961

  Fragments arranged by size: 

                1961     897     214
 
StuI AGG'CCT

Cuts at:      0    2573    2912    3072 
   Size:       2573     339     160

  Fragments arranged by size: 

                2573     339     160
 
StyI C'CwwG_G

Cuts at:      0    1640    1862    2416    2759    3072 
   Size:       1640     222     554     343     313

  Fragments arranged by size: 

                1640     554     343     313     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     650    1383    3072 
   Size:        650     733    1689

  Fragments arranged by size: 

                1689     733     650
 
TatI w'GTAC_w

Cuts at:      0     668     749    1103    1249    1319    1797    2542    2722 
   Size:        668      81     354     146      70     478     745     180

Cuts at:   2722    3072 
   Size:        350

  Fragments arranged by size: 

                 745     668     478     354     350     180     146      81
                  70
 
XbaI T'CTAG_A

Cuts at:      0    2097    3072 
   Size:       2097     975
 
XmnI GAAnn'nnTTC

Cuts at:      0    2176    3072 
   Size:       2176     896
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NsiI     NspI     PfoI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI    PvuII     SacI     ScaI     SfcI     SfoI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AhdI     AloI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BciVI     BglI    BglII     BlpI
     BmtI     BsaI    BsaAI    BseYI    BsiEI    BsiWI     BsmI    BspEI
    BsrBI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
     DrdI     EagI     EciI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MluI     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI
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