Restriction map for pF1KSDA1886
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AocI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BbvCI       BcgI        BclI        BfrI        
BglII       BlnI        BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BsrDI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MfeI        MluI        MroI        MunI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SalI        SapI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        TfiI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII       1157  ACATGT               A^C[GA][CT]GT
Alw44I       1621  GTGCAC               G^TGCAC
Aor51HI      2102  AGCGCT               AGC^GCT
ApaLI        1621  GTGCAC               G^TGCAC
BamHI        1817  GGATCC               G^GATCC
BbsI         1342  GTCTTC               GAAGAC(2/6)
BpuAI        1342  GTCTTC               GAAGAC(2/6)
BsaBI        2013  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I      323  TGTACA               T^GTACA
BspHI        1839  TCATGA               T^CATGA
BspLU11I     1157  ACATGT               A^CATGT
BsrGI         323  TGTACA               T^GTACA
BssSI        1420  CACGAG               CACGAG(-5/-1)
BstEII       2425  GGTCACC              G^GT[ACGT]ACC
BstPI        2425  GGTCACC              G^GT[ACGT]ACC
BstYI        1817  GGATCC               [GA]^GATC[CT]
DraIII         44  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1603  CTCTTC               CTCTTC(1/4)
Eco47III     2102  AGCGCT               AGC^GCT
EcoO65I      2425  GGTCACC              G^GT[ACGT]ACC
Ksp632I      1603  CTCTTC               CTCTTC(1/4)
KspI          330  CCGCGG               CCGC^GG
MamI         2013  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1817  GGATCC               [GA]^GATC[CT]
MseI          572  TTAA                 T^TAA
NaeI          102  GCCGGC               GCC^GGC
NcoI          179  CCATGG               C^CATGG
NdeI           65  CATATG               CA^TATG
NgoMIV        102  GCCGGC               G^CCGGC
PflMI         162  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1839  TCATGA               T^CATGA
SacII         330  CCGCGG               CCGC^GG
SbfI          187  CCTGCAGG             CCTGCA^GG
SmlI         1201  CTCAAG               C^T[CT][GA]AG
Sse8387I      187  CCTGCAGG             CCTGCA^GG
SspBI         323  TGTACA               T^GTACA
StuI         1384  AGGCCT               AGG^CCT
Tru9I         572  TTAA                 T^TAA
Tsp509I      1403  AATT                 ^AATT
Van91I        162  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1817  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccI         1363  GTCTAC               GT^[AC][GT]AC
             2388  GTCTAC              
AcyI          292  GGCGCC               G[GA]^CG[CT]C
             1018  GGCGCC              
AhdI          785  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1867  GACCTGGAGTC         
AspEI         785  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1867  GACCTGGAGTC         
AspI          948  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1357  GACTCAGTC           
BalI         1447  TGGCCA               TGG^CCA
             2235  TGGCCA              
BbeI          292  GGCGCC               GGCGC^C
             1018  GGCGCC              
BbrPI        2028  CACGTG               CAC^GTG
             2128  CACGTG              
BciVI         750  GTATCC               GTATCC(6/5)
              816  GTATCC              
BfaI          611  CTAG                 C^TAG
             1618  CTAG                
BlpI          534  GCTGAGC              GC^T[ACGT]AGC
             1681  GCTGAGC             
Bpu1102I      534  GCTGAGC              GC^T[ACGT]AGC
             1681  GCTGAGC             
BsaHI         292  GGCGCC               G[GA]^CG[CT]C
             1018  GGCGCC              
BsmAI        1355  GAGAC                GTCTC(1/5)
             1182  GTCTC               
BsrFI         102  GCCGGC               [GA]^CCGG[CT]
             1427  ACCGGC              
BstXI        2141  CCAGCGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2246  CCAGCTTCCTGG        
CelII         534  GCTGAGC              GC^T[ACGT]AGC
             1681  GCTGAGC             
Cfr10I        102  GCCGGC               [GA]^CCGG[CT]
             1427  ACCGGC              
EaeI         1447  TGGCCA               [CT]^GGCC[GA]
             2235  TGGCCA              
Eam1105I      785  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1867  GACCTGGAGTC         
Hin1I         292  GGCGCC               G[GA]^CG[CT]C
             1018  GGCGCC              
KasI          292  GGCGCC               G^GCGCC
             1018  GGCGCC              
MaeI          611  CTAG                 C^TAG
             1618  CTAG                
MboII        1343  TCTTC                GAAGA(8/7)
             1604  TCTTC               
MluNI        1447  TGGCCA               TGG^CCA
             2235  TGGCCA              
MscI         1447  TGGCCA               TGG^CCA
             2235  TGGCCA              
NarI          292  GGCGCC               GG^CGCC
             1018  GGCGCC              
PflFI         948  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1357  GACTCAGTC           
PmaCI        2028  CACGTG               CAC^GTG
             2128  CACGTG              
PmlI         2028  CACGTG               CAC^GTG
             2128  CACGTG              
SfiI          379  GGCCCGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              658  GGCCCCTCTGGCC       
SfoI          292  GGCGCC               GGC^GCC
             1018  GGCGCC              
TaqI         1493  TCGA                 T^CGA
             2021  TCGA                
Tth111I       948  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1357  GACTCAGTC           
TthHB8I      1493  TCGA                 T^CGA
             2021  TCGA                
XcmI          491  CCACAGCCACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2591  CCACAGGCGTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TseI        Tsp45I      TspRI       XmaCI       
XmaI        
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