Restriction map for pF1KSDA1886
 (Linear) (Six-base) MAPSORT of: KIAA1886.seq  Check: 2866  from: 1  to: 2760



KIAA1886.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AccI GT'mk_AC

Cuts at:      0    1364    2389    2760 
   Size:       1364    1025     371

  Fragments arranged by size: 

                1364    1025     371
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305    1555    1698    1813    2119    2363    2565    2760 
   Size:        305    1250     143     115     306     244     202     195

  Fragments arranged by size: 

                1250     306     305     244     202     195     143     115
            
 
AfeI AGC'GCT

Cuts at:      0    2104    2760 
   Size:       2104     656
 
AflIII A'CryG_T

Cuts at:      0    1157    2760 
   Size:       1157    1603
 
AhdI GACnn_n'nnGTC

Cuts at:      0     790    1872    2760 
   Size:        790    1082     888

  Fragments arranged by size: 

                1082     888     790
 
AleI CACnn'nnGTG

Cuts at:      0     130    2596    2760 
   Size:        130    2466     164

  Fragments arranged by size: 

                2466     164     130
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     296     911    2760 
   Size:        296     615    1849

  Fragments arranged by size: 

                1849     615     296
 
AlwNI CAG_nnn'CTG

Cuts at:      0     264     448     475     534     585     735     777     834 
   Size:        264     184      27      59      51     150      42      57

Cuts at:    834     957    2215    2760 
   Size:        123    1258     545

  Fragments arranged by size: 

                1258     545     264     184     150     123      59      57
                  51      42      27
 
ApaI G_GGCC'C

Cuts at:      0     187     351     661    1748    2173    2487    2609    2704 
   Size:        187     164     310    1087     425     314     122      95

Cuts at:   2704    2760 
   Size:         56

  Fragments arranged by size: 

                1087     425     314     310     187     164     122      95
                  56
 
ApaLI G'TGCA_C

Cuts at:      0    1621    2760 
   Size:       1621    1139
 
AvaI C'yCGr_G

Cuts at:      0     381     602     695    1086    1832    2325    2760 
   Size:        381     221      93     391     746     493     435

  Fragments arranged by size: 

                 746     493     435     391     381     221      93
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     733     766    2760 
   Size:        733      33    1994

  Fragments arranged by size: 

                1994     733      33
 
BamHI G'GATC_C

Cuts at:      0    1817    2760 
   Size:       1817     943
 
BanI G'GyrC_C

Cuts at:      0     292    1018    1723    1735    2415    2760 
   Size:        292     726     705      12     680     345

  Fragments arranged by size: 

                 726     705     680     345     292      12
 
BanII G_rGCy'C

Cuts at:      0     187     351     661    1304    1748    2122    2173    2487 
   Size:        187     164     310     643     444     374      51     314

Cuts at:   2487    2609    2704    2760 
   Size:        122      95      56

  Fragments arranged by size: 

                 643     444     374     314     310     187     164     122
                  95      56      51
 
BbeI G_GCGC'C

Cuts at:      0     296    1022    2760 
   Size:        296     726    1738

  Fragments arranged by size: 

                1738     726     296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1335    2760 
   Size:       1335    1425
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     761     827    2760 
   Size:        761      66    1933

  Fragments arranged by size: 

                1933     761      66
 
BglI GCCn_nnn'nGGC

Cuts at:      0     335     386     502     665    1058    1553    2088    2632 
   Size:        335      51     116     163     393     495     535     544

Cuts at:   2632    2760 
   Size:        128

  Fragments arranged by size: 

                 544     535     495     393     335     163     128     116
                  51
 
BlpI GC'TnA_GC

Cuts at:      0     535    1682    2760 
   Size:        535    1147    1078

  Fragments arranged by size: 

                1147    1078     535
 
Bme1580I G_kGCm'C

Cuts at:      0     187     351     661     923    1625    1748    1801    2051 
   Size:        187     164     310     262     702     123      53     250

Cuts at:   2051    2173    2487    2609    2704    2760 
   Size:        122     314     122      95      56

  Fragments arranged by size: 

                 702     314     310     262     250     187     164     123
                 122     122      95      56      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     861     945    1091    2760 
   Size:        861      84     146    1669

  Fragments arranged by size: 

                1669     861     146      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1318    1414    1891    1963    1972    2474    2642 
   Size:        496     822      96     477      72       9     502     168

Cuts at:   2642    2760 
   Size:        118

  Fragments arranged by size: 

                 822     502     496     477     168     118      96      72
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0    2556    2760 
   Size:       2556     204
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1186    2760 
   Size:       1186    1574
 
BsaAI yAC'GTr

Cuts at:      0     309    2030    2130    2760 
   Size:        309    1721     100     630

  Fragments arranged by size: 

                1721     630     309     100
 
BsaBI GATnn'nnATC

Cuts at:      0    2017    2760 
   Size:       2017     743
 
BsaHI Gr'CG_yC

Cuts at:      0     293    1019    2760 
   Size:        293     726    1741

  Fragments arranged by size: 

                1741     726     293
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1201    1231    2696    2726    2760 
   Size:       1201      30    1465      30      34

  Fragments arranged by size: 

                1465    1201      34      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1648    2023    2092    2760 
   Size:       1648     375      69     668

  Fragments arranged by size: 

                1648     668     375      69
 
BseYI C'CCAG_C

Cuts at:      0     770     856     877    1971    2140    2221    2296    2362 
   Size:        770      86      21    1094     169      81      75      66

Cuts at:   2362    2498    2523    2611    2760 
   Size:        136      25      88     149

  Fragments arranged by size: 

                1094     770     169     149     136      88      86      81
                  75      66      25      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1480    2052    2488    2760 
   Size:        331    1149     572     436     272

  Fragments arranged by size: 

                1149     572     436     331     272
 
BsiHKAI G_wGCw'C

Cuts at:      0    1625    1688    2135    2760 
   Size:       1625      63     447     625

  Fragments arranged by size: 

                1625     625     447      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     351     661     923    1304    1625    1688    1748 
   Size:        187     164     310     262     381     321      63      60

Cuts at:   1748    1801    2051    2122    2135    2173    2487    2609    2704 
   Size:         53     250      71      13      38     314     122      95

Cuts at:   2704    2760 
   Size:         56

  Fragments arranged by size: 

                 381     321     314     310     262     250     187     164
                 122      95      71      63      60      56      53      38
                  13
 
BspHI T'CATG_A

Cuts at:      0    1839    2760 
   Size:       1839     921
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      33    1452    1889    2413    2663    2744    2760 
   Size:         33    1419     437     524     250      81      16

  Fragments arranged by size: 

                1419     524     437     250      81      33      16
 
BsrBI CCG'CTC

Cuts at:      0    1485    1640    1899    2760 
   Size:       1485     155     259     861

  Fragments arranged by size: 

                1485     861     259     155
 
BsrFI r'CCGG_y

Cuts at:      0     102    1427    2760 
   Size:        102    1325    1333

  Fragments arranged by size: 

                1333    1325     102
 
BsrGI T'GTAC_A

Cuts at:      0     323    2760 
   Size:        323    2437
 
BssSI C'ACGA_G

Cuts at:      0    1420    2760 
   Size:       1420    1340
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     448     475     735     834     927    1620    2760 
   Size:        448      27     260      99      93     693    1140

  Fragments arranged by size: 

                1140     693     448     260      99      93      27
 
BstEII G'GTnAC_C

Cuts at:      0    2425    2760 
   Size:       2425     335
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2148    2253    2760 
   Size:       2148     105     507

  Fragments arranged by size: 

                2148     507     105
 
BstYI r'GATC_y

Cuts at:      0    1817    2760 
   Size:       1817     943
 
BtgI C'CryG_G

Cuts at:      0     179     330     500     974    2760 
   Size:        179     151     170     474    1786

  Fragments arranged by size: 

                1786     474     179     170     151
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     565    1214    1298    2760 
   Size:        565     649      84    1462

  Fragments arranged by size: 

                1462     649     565      84
 
BtsI GCAGTG_nn'

Cuts at:      0     473     775    1105    1686    2760 
   Size:        473     302     330     581    1074

  Fragments arranged by size: 

                1074     581     473     330     302
 
DraIII CAC_nnn'GTG

Cuts at:      0      49    2760 
   Size:         49    2711
 
EaeI y'GGCC_r

Cuts at:      0    1447    2235    2760 
   Size:       1447     788     525

  Fragments arranged by size: 

                1447     788     525
 
EarI CTCTTCn'nnn_

Cuts at:      0    1609    2760 
   Size:       1609    1151
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     231    1297    2760 
   Size:        231    1066    1463

  Fragments arranged by size: 

                1463    1066     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     496    1318    1414    1555    1698    1813    1891 
   Size:        305     191     822      96     141     143     115      78

Cuts at:   1891    1963    1972    2119    2363    2474    2565    2642    2760 
   Size:         72       9     147     244     111      91      77     118

  Fragments arranged by size: 

                 822     305     244     191     147     143     141     118
                 115     111      96      91      78      77      72       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     937    1812    1832    2591    2760 
   Size:        937     875      20     759     169

  Fragments arranged by size: 

                 937     875     759     169      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     184     347     348     387     598     607     658    1653 
   Size:        184     163       1      39     211       9      51     995

Cuts at:   1653    1947    2170    2257    2483    2484    2494    2560    2605 
   Size:        294     223      87     226       1      10      66      45

Cuts at:   2605    2606    2634    2760 
   Size:          1      28     126

  Fragments arranged by size: 

                 995     294     226     223     211     184     163     126
                  87      66      51      45      39      28      10       9
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1203    1265    1728    2760 
   Size:       1203      62     463    1032

  Fragments arranged by size: 

                1203    1032     463      62
 
HaeII r_GCGC'y

Cuts at:      0      95     296    1022    1671    2106    2180    2760 
   Size:         95     201     726     649     435      74     580

  Fragments arranged by size: 

                 726     649     580     435     201      95      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     776     808    1165    1197    1346    1378    1858    1890 
   Size:        776      32     357      32     149      32     480      32

Cuts at:   1890    2674    2706    2760 
   Size:        784      32      54

  Fragments arranged by size: 

                 784     776     480     357     149      54      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0     292    1018    2760 
   Size:        292     726    1742

  Fragments arranged by size: 

                1742     726     292
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2498    2760 
   Size:       2498     262
 
MscI TGG'CCA

Cuts at:      0    1449    2237    2760 
   Size:       1449     788     523

  Fragments arranged by size: 

                1449     788     523
 
MslI CAynn'nnrTG

Cuts at:      0      50     130    2596    2760 
   Size:         50      80    2466     164

  Fragments arranged by size: 

                2466     164      80      50
 
MspA1I CmG'CkG

Cuts at:      0     332     445     454     622    1350    1721    2036    2502 
   Size:        332     113       9     168     728     371     315     466

Cuts at:   2502    2760 
   Size:        258

  Fragments arranged by size: 

                 728     466     371     332     315     258     168     113
                   9
 
NaeI GCC'GGC

Cuts at:      0     104    2760 
   Size:        104    2656
 
NarI GG'CG_CC

Cuts at:      0     293    1019    2760 
   Size:        293     726    1741

  Fragments arranged by size: 

                1741     726     293
 
NcoI C'CATG_G

Cuts at:      0     179    2760 
   Size:        179    2581
 
NdeI CA'TA_TG

Cuts at:      0      66    2760 
   Size:         66    2694
 
NgoMIV G'CCGG_C

Cuts at:      0     102    2760 
   Size:        102    2658
 
NspI r_CATG'y

Cuts at:      0     409    1161    1543    1971    2067    2619    2760 
   Size:        409     752     382     428      96     552     141

  Fragments arranged by size: 

                 752     552     428     409     382     141      96
 
PasI CC'CwG_GG

Cuts at:      0     343     883     916    1741    2166    2760 
   Size:        343     540      33     825     425     594

  Fragments arranged by size: 

                 825     594     540     425     343      33
 
PciI A'CATG_T

Cuts at:      0    1157    2760 
   Size:       1157    1603
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168    2760 
   Size:        168    2592
 
PfoI T'CCnGG_A

Cuts at:      0     512    1295    2660    2760 
   Size:        512     783    1365     100

  Fragments arranged by size: 

                1365     783     512     100
 
PmlI CAC'GTG

Cuts at:      0    2030    2130    2760 
   Size:       2030     100     630

  Fragments arranged by size: 

                2030     630     100
 
PpuMI rG'GwC_Cy

Cuts at:      0     598    2494    2560    2760 
   Size:        598    1896      66     200

  Fragments arranged by size: 

                1896     598     200      66
 
PsiI TTA'TAA

Cuts at:      0     989    2760 
   Size:        989    1771
 
PspOMI G'GGCC_C

Cuts at:      0     183     347     657    1744    2169    2483    2605    2700 
   Size:        183     164     310    1087     425     314     122      95

Cuts at:   2700    2760 
   Size:         60

  Fragments arranged by size: 

                1087     425     314     310     183     164     122      95
                  60
 
PstI C_TGCA'G

Cuts at:      0     192     453     590     938    1316    1616    1652    1886 
   Size:        192     261     137     348     378     300      36     234

Cuts at:   1886    2201    2760 
   Size:        315     559

  Fragments arranged by size: 

                 559     378     348     315     300     261     234     192
                 137      36
 
PvuII CAG'CTG

Cuts at:      0     445     454    1350    2036    2502    2760 
   Size:        445       9     896     686     466     258

  Fragments arranged by size: 

                 896     686     466     445     258       9
 
SacII CC_GC'GG

Cuts at:      0     333    2760 
   Size:        333    2427
 
SbfI CC_TGCA'GG

Cuts at:      0     192    2760 
   Size:        192    2568
 
SfcI C'TryA_G

Cuts at:      0     188     449     586     934    1047    1312    1612    1648 
   Size:        188     261     137     348     113     265     300      36

Cuts at:   1648    1882    2197    2760 
   Size:        234     315     563

  Fragments arranged by size: 

                 563     348     315     300     265     261     234     188
                 137     113      36
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     386     665    2760 
   Size:        386     279    2095

  Fragments arranged by size: 

                2095     386     279
 
SfoI GGC'GCC

Cuts at:      0     294    1020    2760 
   Size:        294     726    1740

  Fragments arranged by size: 

                1740     726     294
 
SmaI CCC'GGG

Cuts at:      0     383     604     697    1088    2327    2760 
   Size:        383     221      93     391    1239     433

  Fragments arranged by size: 

                1239     433     391     383     221      93
 
SmlI C'TyrA_G

Cuts at:      0    1201    2760 
   Size:       1201    1559
 
StuI AGG'CCT

Cuts at:      0    1386    2760 
   Size:       1386    1374
 
StyI C'CwwG_G

Cuts at:      0      79     179     677    2478    2540    2760 
   Size:         79     100     498    1801      62     220

  Fragments arranged by size: 

                1801     498     220     100      79      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     870    1143    2301    2760 
   Size:        870     273    1158     459

  Fragments arranged by size: 

                1158     870     459     273
 
TatI w'GTAC_w

Cuts at:      0     323    1964    2760 
   Size:        323    1641     796

  Fragments arranged by size: 

                1641     796     323
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1615    1647    2430    2462    2696    2728    2760 
   Size:       1615      32     783      32     234      32      32

  Fragments arranged by size: 

                1615     783     234      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     951    1360    2760 
   Size:        951     409    1400

  Fragments arranged by size: 

                1400     951     409
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     498    2598    2760 
   Size:        498    2100     162

  Fragments arranged by size: 

                2100     498     162
 
XmaI C'CCGG_G

Cuts at:      0     381     602     695    1086    2325    2760 
   Size:        381     221      93     391    1239     435

  Fragments arranged by size: 

                1239     435     391     381     221      93
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI     PsiI   PspOMI     PstI    PvuII    SacII     SbfI
     SfcI     SfiI     SfoI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII    BbvCI     BcgI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI     BsaI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI   BssHII    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NheI     NotI     NruI     NsiI     PacI     PmeI     PpiI
    PshAI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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