Restriction map for pF1KSDA1872
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbrPI       
BcgI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MspA1I      MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII         23  TCCGGA               T^CCGGA
AcsI          153  AAATTT               [GA]^AATT[CT]
AcyI          452  GGCGCC               G[GA]^CG[CT]C
AflIII        936  ACACGT               A^C[GA][CT]GT
Alw44I        895  GTGCAC               G^TGCAC
ApaLI         895  GTGCAC               G^TGCAC
ApoI          153  AAATTT               [GA]^AATT[CT]
AspI          952  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          391  CCCGGG               C^[CT]CG[GA]G
BanII         667  GAGCTC               G[GA]GC[CT]^C
BbeI          452  GGCGCC               GGCGC^C
BbvCI         679  GCTGAGG              CCTCAGC(-5/-2)
BciVI         255  GGATAC               GTATCC(6/5)
BglII          66  AGATCT               A^GATCT
BsaAI         892  TACGTG               [CT]AC^GT[GA]
BsaHI         452  GGCGCC               G[GA]^CG[CT]C
BseAI          23  TCCGGA               T^CCGGA
BseRI         137  CTCCTC               GAGGAG(10/8)
BsgI          502  CTGCAC               GTGCAG(16/14)
BsiEI         771  CGACCG               CG[GA][CT]^CG
BsmBI          91  GAGACG               CGTCTC(1/5)
BsoBI         391  CCCGGG               C^[CT]CG[GA]G
BspEI          23  TCCGGA               T^CCGGA
BspMI         500  ACCTGC               ACCTGC(4/8)
BssSI         819  CTCGTG               CACGAG(-5/-1)
BstEII        531  GGTCACC              G^GT[ACGT]ACC
BstPI         531  GGTCACC              G^GT[ACGT]ACC
EcoO65I       531  GGTCACC              G^GT[ACGT]ACC
Hin1I         452  GGCGCC               G[GA]^CG[CT]C
HphI          533  TCACC                GGTGA(8/7)
KasI          452  GGCGCC               G^GCGCC
MaeIII        532  GTCAC                ^GT[ACGT]AC
MroI           23  TCCGGA               T^CCGGA
MseI          278  TTAA                 T^TAA
NarI          452  GGCGCC               GG^CGCC
PflFI         952  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          313  GACTC                GAGTC(4/5)
PpuMI         229  GGGTCCC              [GA]G^G[AT]CC[CT]
SacI          667  GAGCTC               GAGCT^C
SfoI          452  GGCGCC               GGC^GCC
SmaI          391  CCCGGG               CCC^GGG
StuI          558  AGGCCT               AGG^CCT
TfiI          737  GAATC                G^A[AT]TC
Tru9I         278  TTAA                 T^TAA
Tsp45I        532  GTCAC                ^GT[GC]AC
Tsp509I       154  AATT                 ^AATT
Tth111I       952  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           17  CCACGCTCCGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         391  CCCGGG               C^CCGGG
XmaI          391  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         402  CGCG                 CG^CG
             1002  CGCG                
Asp700I        64  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              636  GAACCTCTTC          
AvaII           5  GGACC                G^G[AT]CC
              230  GGTCC               
BalI          371  TGGCCA               TGG^CCA
              842  TGGCCA              
BclI          470  TGATCA               T^GATCA
              803  TGATCA              
BsaWI          23  TCCGGA               [AT]^CCGG[AT]
              773  ACCGGA              
BstUI         402  CGCG                 CG^CG
             1002  CGCG                
DraII         229  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1006  AGGCCCC             
DsaI          368  CCGTGG               C^C[GA][CT]GG
              537  CCACGG              
EaeI          371  TGGCCA               [CT]^GGCC[GA]
              842  TGGCCA              
EcoO109I      229  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1006  AGGCCCC             
FbaI          470  TGATCA               T^GATCA
              803  TGATCA              
HaeII         439  AGCGCC               [GA]GCGC^[CT]
              452  GGCGCC              
HinfI         313  GACTC                G^A[ACGT]TC
              737  GAATC               
MluNI         371  TGGCCA               TGG^CCA
              842  TGGCCA              
MscI          371  TGGCCA               TGG^CCA
              842  TGGCCA              
MvnI          402  CGCG                 CG^CG
             1002  CGCG                
MwoI          453  GCGCCTGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              723  GCGGAGGATGC         
NspI          386  ACATGC               [GA]CATG^[CT]
             1106  GCATGT              
SfaNI         729  GATGC                GCATC(5/9)
              168  GCATC               
TaqI          293  TCGA                 T^CGA
              770  TCGA                
TspRI         467  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1061  CAGTG               
TthHB8I       293  TCGA                 T^CGA
              770  TCGA                
XmnI           64  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              636  GAACCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BanI        BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
ItaI        Ksp632I     MaeII       MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       TseI        XhoII       
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