Restriction map for pF1KSDA1872
 (Linear) (Six-base) MAPSORT of: KIAA1872.seq  Check: 1536  from: 1  to: 1113



KIAA1872.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769     814    1003    1058    1113 
   Size:        769      45     189      55      55

  Fragments arranged by size: 

                 769     189      55      55      45
 
AflIII A'CryG_T

Cuts at:      0     936    1113 
   Size:        936     177
 
AleI CACnn'nnGTG

Cuts at:      0     367    1113 
   Size:        367     746
 
ApaLI G'TGCA_C

Cuts at:      0     895    1113 
   Size:        895     218
 
ApoI r'AATT_y

Cuts at:      0     153    1113 
   Size:        153     960
 
AvaI C'yCGr_G

Cuts at:      0     391    1113 
   Size:        391     722
 
BanI G'GyrC_C

Cuts at:      0     452     709    1056    1113 
   Size:        452     257     347      57

  Fragments arranged by size: 

                 452     347     257      57
 
BanII G_rGCy'C

Cuts at:      0     671    1113 
   Size:        671     442
 
BbeI G_GCGC'C

Cuts at:      0     456    1113 
   Size:        456     657
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131     187     362     570     914    1113 
   Size:        131      56     175     208     344     199

  Fragments arranged by size: 

                 344     208     199     175     131      56
 
BbvCI CC'TCA_GC

Cuts at:      0     680    1113 
   Size:        680     433
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     249    1113 
   Size:        249     864
 
BclI T'GATC_A

Cuts at:      0     470     803    1113 
   Size:        470     333     310

  Fragments arranged by size: 

                 470     333     310
 
BglII A'GATC_T

Cuts at:      0      66    1113 
   Size:         66    1047
 
Bme1580I G_kGCm'C

Cuts at:      0     899    1113 
   Size:        899     214
 
BmgBI CAC'GTC

Cuts at:      0      42     939    1113 
   Size:         42     897     174

  Fragments arranged by size: 

                 897     174      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     330    1113 
   Size:        330     783
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     367     934    1113 
   Size:        340      27     567     179

  Fragments arranged by size: 

                 567     340     179      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     680    1113 
   Size:        680     433
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289     301    1089    1113 
   Size:        289      12     788      24

  Fragments arranged by size: 

                 788     289      24      12
 
BsaAI yAC'GTr

Cuts at:      0     894    1113 
   Size:        894     219
 
BsaHI Gr'CG_yC

Cuts at:      0     453    1113 
   Size:        453     660
 
BsaWI w'CCGG_w

Cuts at:      0      23     773    1113 
   Size:         23     750     340

  Fragments arranged by size: 

                 750     340      23
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     120     150    1113 
   Size:        120      30     963

  Fragments arranged by size: 

                 963     120      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     128    1113 
   Size:        128     985
 
BseYI C'CCAG_C

Cuts at:      0    1010    1113 
   Size:       1010     103
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487    1113 
   Size:        487     626
 
BsiEI CG_ry'CG

Cuts at:      0     774    1113 
   Size:        774     339
 
BsiHKAI G_wGCw'C

Cuts at:      0     491     671     899    1113 
   Size:        491     180     228     214

  Fragments arranged by size: 

                 491     228     214     180
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      85    1113 
   Size:         85    1028
 
Bsp1286I G_dGCh'C

Cuts at:      0     491     671     899    1113 
   Size:        491     180     228     214

  Fragments arranged by size: 

                 491     228     214     180
 
BspEI T'CCGG_A

Cuts at:      0      23    1113 
   Size:         23    1090
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     509    1113 
   Size:        509     604
 
BssSI C'ACGA_G

Cuts at:      0     819    1113 
   Size:        819     294
 
BstEII G'GTnAC_C

Cuts at:      0     531    1113 
   Size:        531     582
 
BstYI r'GATC_y

Cuts at:      0      66     405     884     944    1113 
   Size:         66     339     479      60     169

  Fragments arranged by size: 

                 479     339     169      66      60
 
BtgI C'CryG_G

Cuts at:      0     368     537    1113 
   Size:        368     169     576

  Fragments arranged by size: 

                 576     368     169
 
EaeI y'GGCC_r

Cuts at:      0     371     842    1113 
   Size:        371     471     271

  Fragments arranged by size: 

                 471     371     271
 
EarI CTCTTCn'nnn_

Cuts at:      0      83     646     965    1034    1113 
   Size:         83     563     319      69      79

  Fragments arranged by size: 

                 563     319      83      79      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     477     738    1113 
   Size:        477     261     375

  Fragments arranged by size: 

                 477     375     261
 
EcoICRI GAG'CTC

Cuts at:      0     669    1113 
   Size:        669     444
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     367     769     814     934    1003    1058    1113 
   Size:        340      27     402      45     120      69      55      55

  Fragments arranged by size: 

                 402     340     120      69      55      55      45      27
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     230    1007    1113 
   Size:        230     777     106

  Fragments arranged by size: 

                 777     230     106
 
HaeII r_GCGC'y

Cuts at:      0     443     456    1113 
   Size:        443      13     657

  Fragments arranged by size: 

                 657     443      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150    1113 
   Size:        118      32     963

  Fragments arranged by size: 

                 963     118      32
 
KasI G'GCGC_C

Cuts at:      0     452    1113 
   Size:        452     661
 
MscI TGG'CCA

Cuts at:      0     373     844    1113 
   Size:        373     471     269

  Fragments arranged by size: 

                 471     373     269
 
MslI CAynn'nnrTG

Cuts at:      0     367     526     694     928    1113 
   Size:        367     159     168     234     185

  Fragments arranged by size: 

                 367     234     185     168     159
 
NarI GG'CG_CC

Cuts at:      0     453    1113 
   Size:        453     660
 
NspI r_CATG'y

Cuts at:      0     390    1110    1113 
   Size:        390     720       3

  Fragments arranged by size: 

                 720     390       3
 
PpuMI rG'GwC_Cy

Cuts at:      0     230    1113 
   Size:        230     883
 
SacI G_AGCT'C

Cuts at:      0     671    1113 
   Size:        671     442
 
SanDI GG'GwC_CC

Cuts at:      0     230    1113 
   Size:        230     883
 
SfoI GGC'GCC

Cuts at:      0     454    1113 
   Size:        454     659
 
SmaI CCC'GGG

Cuts at:      0     393    1113 
   Size:        393     720
 
StuI AGG'CCT

Cuts at:      0     560    1113 
   Size:        560     553
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325     550    1113 
   Size:        325     225     563

  Fragments arranged by size: 

                 563     325     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     120     152    1113 
   Size:        120      32     961

  Fragments arranged by size: 

                 961     120      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     955    1113 
   Size:        955     158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      24    1113 
   Size:         24    1089
 
XmaI C'CCGG_G

Cuts at:      0     391    1113 
   Size:        391     722
 
XmnI GAAnn'nnTTC

Cuts at:      0      68     640    1113 
   Size:         68     572     473

  Fragments arranged by size: 

                 572     473      68
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    ApaLI     ApoI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII Bme1580I    BmgBI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BssSI   BstEII    BstYI     BtgI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MscI     MslI
     NarI     NspI    PpuMI     SacI    SanDI     SfoI     SmaI     StuI
    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BfrBI     BglI     BlpI     BmrI
     BmtI    BpuEI    BsaBI    BsiWI     BsmI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TatI     XbaI     XhoI     ZraI
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