Restriction map for pF1KSDA1830
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
Asp718I     AspEI       AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraII       DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MfeI        
MluI        MroI        MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI           30  GCGG                 CCGC(-3/-1)
AseI         1006  ATTAAT               AT^TAAT
AsnI         1006  ATTAAT               AT^TAAT
AspHI        1116  GAGCTC               G[AT]GC[AT]^C
AspI          991  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          988  GAAGAC               GAAGAC(2/6)
BpmI          224  CTCCAG               CTGGAG(16/14)
BpuAI         988  GAAGAC               GAAGAC(2/6)
BsaBI         646  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          833  GAGACC               GGTCTC(1/5)
BsgI         1324  GTGCAG               GTGCAG(16/14)
BsiHKAI      1116  GAGCTC               G[AT]GC[AT]^C
BsrDI         267  CATTGC               GCAATG(2/0)
BstXI         864  CCACATGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1306  GGATCT               [GA]^GATC[CT]
DraI          103  TTTAAA               TTT^AAA
DraIII       1154  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          151  CCATGG               C^C[GA][CT]GG
HincII        416  GTCAAC               GT[CT]^[GA]AC
HindII        416  GTCAAC               GT[CT]^[GA]AC
HindIII       739  AAGCTT               A^AGCTT
MaeIII        574  GTGAC                ^GT[ACGT]AC
MamI          646  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1306  GGATCT               [GA]^GATC[CT]
MslI          865  CACATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        593  CAGCTG               C[AC]G^C[GT]G
NcoI          151  CCATGG               C^CATGG
NdeI          433  CATATG               CA^TATG
PflFI         991  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         151  CCATGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1006  ATTAAT               AT^TAAT
PvuII         593  CAGCTG               CAG^CTG
SacI         1116  GAGCTC               GAGCT^C
SmlI          202  CTTGAG               C^T[CT][GA]AG
SspI          953  AATATT               AAT^ATT
Tsp45I        574  GTGAC                ^GT[GC]AC
Tth111I       991  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        151  CCATGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1306  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI           52  GTAC                 GT^AC
              931  GTAC                
AlwI          665  GGATC                GGATC(4/5)
             1306  GGATC               
Asp700I       556  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              676  GAAAGGTTTC          
AvaII         343  GGACC                G^G[AT]CC
              996  GGTCC               
BalI          159  TGGCCA               TGG^CCA
              861  TGGCCA              
BfaI          263  CTAG                 C^TAG
              964  CTAG                
BsmFI         320  GGGAC                GGGAC(10/14)
              916  GTCCC               
Cac8I         591  GCCAGC               GC[ACGT]^[ACGT]GC
             1148  GCCTGC              
CfoI           38  GCGC                 GCG^C
              148  GCGC                
DdeI          549  CTCAG                C^T[ACGT]AG
              566  CTGAG               
HhaI           38  GCGC                 GCG^C
              148  GCGC                
HinP1I         38  GCGC                 G^CGC
              148  GCGC                
MaeI          263  CTAG                 C^TAG
              964  CTAG                
MluNI         159  TGGCCA               TGG^CCA
              861  TGGCCA              
MscI          159  TGGCCA               TGG^CCA
              861  TGGCCA              
MwoI          591  GCCAGCTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1317  GCCTACAGTGC         
RsaI           52  GTAC                 GT^AC
              931  GTAC                
TaqI          114  TCGA                 T^CGA
              288  TCGA                
TthHB8I       114  TCGA                 T^CGA
              288  TCGA                
XmnI          556  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              676  GAAAGGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        BanII       BbvI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BstF5I      Cfr13I      DpnII       EaeI        EarI        
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MboI        MboII       
MnlI        MseI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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