Restriction map for pF1KSDA1823
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII        292  ACATGT               A^C[GA][CT]GT
AvaII         539  GGTCC                G^G[AT]CC
BanII         454  GAGCCC               G[GA]GC[CT]^C
BclI         1056  TGATCA               T^GATCA
BfaI          614  CTAG                 C^TAG
BglI          697  GCCAAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          454  GAGCCC               G[AGT]GC[ACT]^C
BsaI          593  GGTCTC               GGTCTC(1/5)
BsgI          401  CTGCAC               GTGCAG(16/14)
Bsp1286I      454  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      838  TGTACA               T^GTACA
BspLU11I      292  ACATGT               A^CATGT
BsrDI         323  CATTGC               GCAATG(2/0)
BsrGI         838  TGTACA               T^GTACA
BsrI          450  ACTGG                ACTGG(1/-1)
BstXI         255  CCATTGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         134  GCATGC               GC[ACGT]^[ACGT]GC
DpnII        1057  GATC                 ^GATC
DraI          514  TTTAAA               TTT^AAA
DraII          27  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          552  CCATGG               C^C[GA][CT]GG
EcoNI         355  CCTTCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       27  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       552  CCATGG               C^C[AT][AT]GG
FbaI         1056  TGATCA               T^GATCA
HphI          463  TCACC                GGTGA(8/7)
MaeI          614  CTAG                 C^TAG
MboI         1057  GATC                 ^GATC
MfeI          272  CAATTG               C^AATTG
MspA1I        707  CAGCTG               C[AC]G^C[GT]G
MunI          272  CAATTG               C^AATTG
NcoI          552  CCATGG               C^CATGG
NdeII        1057  GATC                 ^GATC
NlaIV         453  GGAGCC               GG[ACGT]^[ACGT]CC
PflMI         108  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         707  CAGCTG               CAG^CTG
Sau3AI       1057  GATC                 ^GATC
SexAI         535  ACCAGGT              A^CC[AT]GGT
SfcI          582  CTATAG               C^T[GA][CT]AG
SphI          134  GCATGC               GCATG^C
SspBI         838  TGTACA               T^GTACA
StuI          625  AGGCCT               AGG^CCT
StyI          552  CCATGG               C^C[AT][AT]GG
Van91I        108  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          903  CCATTACCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BpmI          263  CTGGAG               CTGGAG(16/14)
              451  CTGGAG              
BsaJI         552  CCATGG               C^C[ACGT][ACGT]GG
             1038  CCGAGG              
BssSI         671  CACGAG               CACGAG(-5/-1)
              953  CACGAG              
BstF5I        545  CATCC                GGATG(2/0)
              871  GGATG               
CfoI           42  GCGC                 GCG^C
              121  GCGC                
Cfr13I         28  GGGCC                G^G[ACGT]CC
              539  GGTCC               
DdeI            9  CTCAG                C^T[ACGT]AG
             1073  CTCAG               
FokI          545  CATCC                GGATG(9/13)
              871  GGATG               
HhaI           42  GCGC                 GCG^C
              121  GCGC                
HinP1I         42  GCGC                 G^CGC
              121  GCGC                
MwoI           44  GCAAATGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              697  GCCAAGAAGGC         
Sau96I         28  GGGCC                G^G[ACGT]CC
              539  GGTCC               
TspRI         316  CACTG                CA[GC]TG[ACGT][ACGT]^
              910  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BsiYI       BslI        BsmAI       BssKI       BstNI       
EcoRII      Fnu4HI      HaeIII      ItaI        MaeIII      
MboII       MnlI        MseI        MvaI        NlaIII      
NspI        RsaI        ScrFI       Tru9I       TseI        
Tsp45I      Tsp509I     
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