(Circular) (Six-base) MAPSORT of: pF1KSDA1817.seq Check: 4350 from: 1 to: 5735 pF1KSDA1817 5735 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 924 1980 2010 2610 924 Size: 1056 30 600 4049 Fragments arranged by size: 4049 1056 600 30 AccI GT'mk_AC Cuts at: 1628 2773 1628 Size: 1145 4590 Acc65I G'GTAC_C Cuts at: 2751 2751 Size: 5735 AclI AA'CG_TT Cuts at: 5575 5575 Size: 5735 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 433 1016 3560 3992 4739 5007 433 Size: 583 2544 432 747 268 1161 Fragments arranged by size: 2544 1161 747 583 432 268 AflIII A'CryG_T Cuts at: 1516 3087 4191 5168 1516 Size: 1571 1104 977 2083 Fragments arranged by size: 2083 1571 1104 977 AgeI A'CCGG_T Cuts at: 4123 4123 Size: 5735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1261 2032 1261 Size: 771 4964 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1121 1153 1121 Size: 32 5703 AlwNI CAG_nnn'CTG Cuts at: 1210 1402 2025 2388 2632 3040 4607 1210 Size: 192 623 363 244 408 1567 2338 Fragments arranged by size: 2338 1567 623 408 363 244 192 ApaI G_GGCC'C Cuts at: 2139 2139 Size: 5735 ApaLI G'TGCA_C Cuts at: 4505 4505 Size: 5735 ApoI r'AATT_y Cuts at: 453 564 2739 5088 5344 453 Size: 111 2175 2349 256 844 Fragments arranged by size: 2349 2175 844 256 111 AseI AT'TA_AT Cuts at: 20 20 Size: 5735 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5735 AvaI C'yCGr_G Cuts at: 767 866 893 1022 1469 1524 1807 2227 2755 Size: 99 27 129 447 55 283 420 528 Cuts at: 2755 767 Size: 3747 Fragments arranged by size: 3747 528 447 420 283 129 99 55 27 BamHI G'GATC_C Cuts at: 2760 2760 Size: 5735 BanI G'GyrC_C Cuts at: 685 1183 1995 2751 3165 3413 3448 685 Size: 498 812 756 414 248 35 2972 Fragments arranged by size: 2972 812 756 498 414 248 35 BanII G_rGCy'C Cuts at: 214 799 880 907 984 1548 1940 2139 2306 Size: 585 81 27 77 564 392 199 167 Cuts at: 2306 2347 2749 3779 214 Size: 41 402 1030 2170 Fragments arranged by size: 2170 1030 585 564 402 392 199 167 81 77 41 27 BbeI G_GCGC'C Cuts at: 3169 3417 3169 Size: 248 5487 BbvCI CC'TCA_GC Cuts at: 178 1420 2513 178 Size: 1242 1093 3400 Fragments arranged by size: 3400 1242 1093 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2218 2252 2218 Size: 34 5701 BciVI GTATCCnnnnn_n' Cuts at: 3633 4394 5735 3633 Size: 761 1341 3633 Fragments arranged by size: 3633 1341 761 BclI T'GATC_A Cuts at: 3255 3255 Size: 5735 BglI GCCn_nnn'nGGC Cuts at: 1313 2134 1313 Size: 821 4914 BglII A'GATC_T Cuts at: 2261 3250 5287 2261 Size: 989 2037 2709 Fragments arranged by size: 2709 2037 989 BlpI GC'TnA_GC Cuts at: 2389 2847 2389 Size: 458 5277 Bme1580I G_kGCm'C Cuts at: 688 1346 2139 2241 3360 3453 4509 688 Size: 658 793 102 1119 93 1056 1914 Fragments arranged by size: 1914 1119 1056 793 658 102 93 BmrI ACTGGGnnnn_n' Cuts at: 425 2947 3028 3363 5507 425 Size: 2522 81 335 2144 653 Fragments arranged by size: 2522 2144 653 335 81 BmtI G_CTAG'C Cuts at: 2287 2287 Size: 5735 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 186 2366 4102 186 Size: 2180 1736 1819 Fragments arranged by size: 2180 1819 1736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 373 422 678 739 1153 1187 1483 1741 373 Size: 49 256 61 414 34 296 258 4367 Fragments arranged by size: 4367 414 296 258 256 61 49 34 Bpu10I CC'TnA_GC Cuts at: 178 1420 2513 178 Size: 1242 1093 3400 Fragments arranged by size: 3400 1242 1093 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1162 2275 2299 2911 3790 4282 4580 1162 Size: 1113 24 612 879 492 298 2317 Fragments arranged by size: 2317 1113 879 612 492 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 174 436 486 745 920 1721 1733 1762 2398 Size: 262 50 259 175 801 12 29 636 Cuts at: 2398 5439 174 Size: 3041 470 Fragments arranged by size: 3041 801 636 470 262 259 175 50 29 12 BsaAI yAC'GTr Cuts at: 1517 2727 3088 1517 Size: 1210 361 4164 Fragments arranged by size: 4164 1210 361 BsaBI GATnn'nnATC Cuts at: 6 3249 5286 6 Size: 3243 2037 455 Fragments arranged by size: 3243 2037 455 BsaHI Gr'CG_yC Cuts at: 3166 3414 4116 5612 3166 Size: 248 702 1496 3289 Fragments arranged by size: 3289 1496 702 248 BsaWI w'CCGG_w Cuts at: 1820 2924 3445 4123 4397 4544 4955 1820 Size: 1104 521 678 274 147 411 2600 Fragments arranged by size: 2600 1104 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 190 220 190 Size: 30 5705 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1825 2530 1825 Size: 705 5030 BseYI C'CCAG_C Cuts at: 331 468 799 826 853 880 1345 1734 2444 Size: 137 331 27 27 27 465 389 710 Cuts at: 2444 3160 3918 4495 4991 331 Size: 716 758 577 496 1075 Fragments arranged by size: 1075 758 716 710 577 496 465 389 331 137 27 27 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 494 511 1267 2528 494 Size: 17 756 1261 3701 Fragments arranged by size: 3701 1261 756 17 BsiEI CG_ry'CG Cuts at: 104 266 1483 3277 3323 4531 104 Size: 162 1217 1794 46 1208 1308 Fragments arranged by size: 1794 1308 1217 1208 162 46 BsiHKAI G_wGCw'C Cuts at: 438 501 727 943 1548 1940 2306 2692 2749 Size: 63 226 216 605 392 366 386 57 Cuts at: 2749 3527 3717 4509 438 Size: 778 190 792 1664 Fragments arranged by size: 1664 792 778 605 392 386 366 226 216 190 63 57 BsmI GAATG_Cn' Cuts at: 1445 1445 Size: 5735 Bsp1286I G_dGCh'C Cuts at: 214 438 501 688 727 799 880 907 943 Size: 224 63 187 39 72 81 27 36 Cuts at: 943 984 1346 1548 1940 2139 2241 2306 2347 Size: 41 362 202 392 199 102 65 41 Cuts at: 2347 2692 2749 3360 3453 3527 3717 3779 4509 Size: 345 57 611 93 74 190 62 730 Cuts at: 4509 214 Size: 1440 Fragments arranged by size: 1440 730 611 392 362 345 224 202 199 190 187 102 93 81 74 72 65 63 62 57 41 41 39 36 27 BspEI T'CCGG_A Cuts at: 4955 4955 Size: 5735 BspHI T'CATG_A Cuts at: 4911 4911 Size: 5735 BspMI ACCTGCnnnn'nnnn_ Cuts at: 156 197 924 1202 1703 1980 2010 2047 2610 Size: 41 727 278 501 277 30 37 563 Cuts at: 2610 2785 2999 3301 3682 156 Size: 175 214 302 381 2209 Fragments arranged by size: 2209 727 563 501 381 302 278 277 214 175 41 37 30 BsrBI CCG'CTC Cuts at: 50 1095 4027 4081 5565 5730 50 Size: 1045 2932 54 1484 165 55 Fragments arranged by size: 2932 1484 1045 165 55 54 BsrDI GCAATG_nn' Cuts at: 3647 3647 Size: 5735 BsrFI r'CCGG_y Cuts at: 929 1316 3733 3914 4123 929 Size: 387 2417 181 209 2541 Fragments arranged by size: 2541 2417 387 209 181 BssSI C'ACGA_G Cuts at: 4006 4364 4006 Size: 358 5377 BstAPI GCAn_nnn'nTGC Cuts at: 1862 1865 1915 1995 2632 3112 1862 Size: 3 50 80 637 480 4485 Fragments arranged by size: 4485 637 480 80 50 3 BstBI TT'CG_AA Cuts at: 110 4096 110 Size: 3986 1749 BstXI CCAn_nnnn'nTGG Cuts at: 841 1698 1788 2146 841 Size: 857 90 358 4430 Fragments arranged by size: 4430 857 358 90 BstYI r'GATC_y Cuts at: 1 1961 2261 2760 3227 3250 3585 3831 4843 Size: 1960 300 499 467 23 335 246 1012 Cuts at: 4843 4929 4941 5287 1 Size: 86 12 346 449 Fragments arranged by size: 1960 1012 499 467 449 346 335 300 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 2057 2057 Size: 5735 BtgI C'CryG_G Cuts at: 782 782 Size: 5735 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3685 3866 4029 3685 Size: 181 163 5391 Fragments arranged by size: 5391 181 163 BtsI GCAGTG_nn' Cuts at: 246 302 402 1801 2218 2978 246 Size: 56 100 1399 417 760 3003 Fragments arranged by size: 3003 1399 760 417 100 56 DraI TTT'AAA Cuts at: 2734 2734 Size: 5735 DrdI GACnn_nn'nnGTC Cuts at: 3441 4299 3441 Size: 858 4877 EaeI y'GGCC_r Cuts at: 263 843 1212 1318 1790 2114 2219 2327 2462 Size: 580 369 106 472 324 105 108 135 Cuts at: 2462 3320 3494 3885 3912 5257 263 Size: 858 174 391 27 1345 741 Fragments arranged by size: 1345 858 741 580 472 391 369 324 174 135 108 106 105 27 EagI C'GGCC_G Cuts at: 263 3320 263 Size: 3057 2678 EarI CTCTTCn'nnn_ Cuts at: 155 489 743 2468 3758 3968 155 Size: 334 254 1725 1290 210 1922 Fragments arranged by size: 1922 1725 1290 334 254 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3943 4253 4399 5545 3943 Size: 310 146 1146 4133 Fragments arranged by size: 4133 1146 310 146 EcoICRI GAG'CTC Cuts at: 1546 1938 2304 2747 1546 Size: 392 366 443 4534 Fragments arranged by size: 4534 443 392 366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 373 422 433 678 739 1016 1153 1187 1483 Size: 49 11 245 61 277 137 34 296 Cuts at: 1483 1741 3560 3992 4739 5007 373 Size: 258 1819 432 747 268 1101 Fragments arranged by size: 1819 1101 747 432 296 277 268 258 245 137 61 49 34 11 EcoNI CCTnn'n_nnAGG Cuts at: 193 193 Size: 5735 EcoO109I rG'GnC_Cy Cuts at: 1000 1027 1255 1607 1702 1777 2012 2874 1000 Size: 27 228 352 95 75 235 862 3861 Fragments arranged by size: 3861 862 352 235 228 95 75 27 EcoRI G'AATT_C Cuts at: 453 2739 453 Size: 2286 3449 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3754 3754 Size: 5735 FseI GG_CCGG'CC Cuts at: 1320 1320 Size: 5735 FspI TGC'GCA Cuts at: 3516 3516 Size: 5735 HaeII r_GCGC'y Cuts at: 2451 3169 3417 4439 5418 2451 Size: 718 248 1022 979 2768 Fragments arranged by size: 2768 1022 979 718 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1465 1497 1594 1626 1924 1956 3577 3607 3609 Size: 32 97 32 298 32 1621 30 2 Cuts at: 3609 3639 1465 Size: 30 3561 Fragments arranged by size: 3561 1621 298 97 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2774 2774 Size: 5735 HindIII A'AGCT_T Cuts at: 217 958 2275 217 Size: 741 1317 3677 Fragments arranged by size: 3677 1317 741 KasI G'GCGC_C Cuts at: 3165 3413 3165 Size: 248 5487 KpnI G_GTAC'C Cuts at: 2755 2755 Size: 5735 MluI A'CGCG_T Cuts at: 5168 5168 Size: 5735 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4406 4590 4406 Size: 184 5551 MscI TGG'CCA Cuts at: 845 2116 2221 2329 2464 3496 845 Size: 1271 105 108 135 1032 3084 Fragments arranged by size: 3084 1271 1032 135 108 105 MslI CAynn'nnrTG Cuts at: 2144 3724 3851 2144 Size: 1580 127 4028 Fragments arranged by size: 4028 1580 127 MspA1I CmG'CkG Cuts at: 468 717 803 830 884 1217 1223 1286 2512 Size: 249 86 27 54 333 6 63 1226 Cuts at: 2512 2632 2846 3160 3520 4533 4778 468 Size: 120 214 314 360 1013 245 1425 Fragments arranged by size: 1425 1226 1013 360 333 314 249 245 214 120 86 63 54 27 6 NaeI GCC'GGC Cuts at: 931 1318 3916 931 Size: 387 2598 2750 Fragments arranged by size: 2750 2598 387 NarI GG'CG_CC Cuts at: 3166 3414 3166 Size: 248 5487 NcoI C'CATG_G Cuts at: 782 782 Size: 5735 NgoMIV G'CCGG_C Cuts at: 929 1316 3914 929 Size: 387 2598 2750 Fragments arranged by size: 2750 2598 387 NheI G'CTAG_C Cuts at: 2283 2283 Size: 5735 NspI r_CATG'y Cuts at: 1266 1653 2358 2788 3288 4195 1266 Size: 387 705 430 500 907 2806 Fragments arranged by size: 2806 907 705 500 430 387 PasI CC'CwG_GG Cuts at: 1644 1658 1644 Size: 14 5721 PciI A'CATG_T Cuts at: 4191 4191 Size: 5735 PflMI CCAn_nnn'nTGG Cuts at: 658 1210 1402 658 Size: 552 192 4991 Fragments arranged by size: 4991 552 192 PmeI GTTT'AAAC Cuts at: 2734 2734 Size: 5735 PmlI CAC'GTG Cuts at: 1517 1517 Size: 5735 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4874 4906 4874 Size: 32 5703 PpuMI rG'GwC_Cy Cuts at: 1027 1702 2012 1027 Size: 675 310 4750 Fragments arranged by size: 4750 675 310 PspOMI G'GGCC_C Cuts at: 2135 2135 Size: 5735 PspXI vC'TCGA_Gb Cuts at: 893 893 Size: 5735 PstI C_TGCA'G Cuts at: 677 794 1112 1222 2021 2044 2637 2782 3467 Size: 117 318 110 799 23 593 145 685 Cuts at: 3467 677 Size: 2945 Fragments arranged by size: 2945 799 685 593 318 145 117 110 23 PvuI CG_AT'CG Cuts at: 104 1483 104 Size: 1379 4356 PvuII CAG'CTG Cuts at: 468 717 803 830 884 1223 1286 2512 2632 Size: 249 86 27 54 339 63 1226 120 Cuts at: 2632 3160 3520 468 Size: 528 360 2683 Fragments arranged by size: 2683 1226 528 360 339 249 120 86 63 54 27 RsrII CG'GwC_CG Cuts at: 3930 3930 Size: 5735 SacI G_AGCT'C Cuts at: 1548 1940 2306 2749 1548 Size: 392 366 443 4534 Fragments arranged by size: 4534 443 392 366 SalI G'TCGA_C Cuts at: 2772 2772 Size: 5735 SanDI GG'GwC_CC Cuts at: 1702 1702 Size: 5735 SapI GCTCTTCn'nnn_ Cuts at: 155 3758 3968 155 Size: 3603 210 1922 Fragments arranged by size: 3603 1922 210 SbfI CC_TGCA'GG Cuts at: 2021 2782 2021 Size: 761 4974 ScaI AGT'ACT Cuts at: 5252 5252 Size: 5735 SfcI C'TryA_G Cuts at: 33 271 623 673 790 1108 1218 1575 2017 Size: 238 352 50 117 318 110 357 442 Cuts at: 2017 2040 2633 2778 3463 4456 4647 33 Size: 23 593 145 685 993 191 1121 Fragments arranged by size: 1121 993 685 593 442 357 352 318 238 191 145 117 110 50 23 SfoI GGC'GCC Cuts at: 3167 3415 3167 Size: 248 5487 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5735 SmaI CCC'GGG Cuts at: 2757 2757 Size: 5735 SmlI C'TyrA_G Cuts at: 866 893 1141 2278 2290 2890 3805 4297 4559 Size: 27 248 1137 12 600 915 492 262 Cuts at: 4559 866 Size: 2042 Fragments arranged by size: 2042 1137 915 600 492 262 248 27 12 SnaBI TAC'GTA Cuts at: 2727 2727 Size: 5735 SpeI A'CTAG_T Cuts at: 67 67 Size: 5735 SphI G_CATG'C Cuts at: 1653 2788 3288 1653 Size: 1135 500 4100 Fragments arranged by size: 4100 1135 500 StuI AGG'CCT Cuts at: 2062 2062 Size: 5735 StyI C'CwwG_G Cuts at: 194 699 782 1667 1697 2465 2869 3223 194 Size: 505 83 885 30 768 404 354 2706 Fragments arranged by size: 2706 885 768 505 404 354 83 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 460 516 646 744 1345 2713 3318 3451 3910 Size: 56 130 98 601 1368 605 133 459 Cuts at: 3910 4119 460 Size: 209 2076 Fragments arranged by size: 2076 1368 605 601 459 209 133 130 98 56 TatI w'GTAC_w Cuts at: 5250 5250 Size: 5735 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2637 2669 5252 5284 2637 Size: 32 2583 32 3088 Fragments arranged by size: 3088 2583 32 32 Tth111I GACn'n_nGTC Cuts at: 1372 3532 1372 Size: 2160 3575 XbaI T'CTAG_A Cuts at: 2766 2766 Size: 5735 XcmI CCAnnnn_n'nnnnTGG Cuts at: 446 3230 446 Size: 2784 2951 XhoI C'TCGA_G Cuts at: 866 893 866 Size: 27 5708 XmaI C'CCGG_G Cuts at: 2755 2755 Size: 5735 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AscI AvrII BaeI BbsI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FspAI HpaI MfeI NdeI NotI NruI NsiI PacI PfoI PshAI PsiI PsrI SacII SexAI SfiI SgrAI SrfI SspI SwaI XmnI ZraI