Restriction map for pF1KSDA1802
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       BstAPI      BstBI       
BstUI       CfoI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRI       FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NarI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SspI        SwaI        Tth111I     XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        100  GGTACC               G^GTACC
AccI          229  GTATAC               GT^[AC][GT]AC
AocI         1325  CCTCAGG              CC^T[ACGT]AGG
Asp718I       100  GGTACC               G^GTACC
AvaI         1594  CCCGAG               C^[CT]CG[GA]G
BanI          100  GGTACC               G^G[CT][GA]CC
BglII        1338  AGATCT               A^GATCT
BlpI         1885  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1885  GCTGAGC              GC^T[ACGT]AGC
BsmI            7  GCATTC               GAATGC(1/-1)
BsoBI        1594  CCCGAG               C^[CT]CG[GA]G
Bsp1407I       84  TGTACA               T^GTACA
BspDI        1561  ATCGAT               AT^CGAT
BsrDI          52  CATTGC               GCAATG(2/0)
BsrFI        2366  GCCGGC               [GA]^CCGG[CT]
BsrGI          84  TGTACA               T^GTACA
Bst1107I      229  GTATAC               GTA^TAC
BstEII       1331  GGTCACC              G^GT[ACGT]ACC
BstPI        1331  GGTCACC              G^GT[ACGT]ACC
BstZ17I       229  GTATAC               GTA^TAC
Bsu36I       1325  CCTCAGG              CC^T[ACGT]AGG
CelII        1885  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2366  GCCGGC               [GA]^CCGG[CT]
ClaI         1561  ATCGAT               AT^CGAT
Eco81I       1325  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1331  GGTCACC              G^GT[ACGT]ACC
EcoRV        1537  GATATC               GAT^ATC
EcoT22I       254  ATGCAT               ATGCA^T
FauI          913  CCCGC                CCCGC(4/6)
HgaI         1882  GACGC                GACGC(5/10)
KpnI          100  GGTACC               GGTAC^C
NaeI         2366  GCCGGC               GCC^GGC
NcoI         2277  CCATGG               C^CATGG
NdeI           94  CATATG               CA^TATG
NgoMIV       2366  GCCGGC               G^CCGGC
NsiI          254  ATGCAT               ATGCA^T
PflMI        1130  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         782  CAGCTG               CAG^CTG
SexAI        1494  ACCAGGT              A^CC[AT]GGT
SmlI         1855  CTCAAG               C^T[CT][GA]AG
SpeI         2020  ACTAGT               A^CTAGT
SphI         1668  GCATGC               GCATG^C
SspBI          84  TGTACA               T^GTACA
StuI          961  AGGCCT               AGG^CCT
TaqI         1562  TCGA                 T^CGA
TthHB8I      1562  TCGA                 T^CGA
Van91I       1130  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2035  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

Asp700I       870  GAAGCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1792  GAAGAACTTC          
AspHI        1312  GAGCTC               G[AT]GC[AT]^C
             1875  GAGCTC              
AvrII         898  CCTAGG               C^CTAGG
              958  CCTAGG              
BlnI          898  CCTAGG               C^CTAGG
              958  CCTAGG              
BsaI          903  GAGACC               GGTCTC(1/5)
             1483  GAGACC              
BsiHKAI      1312  GAGCTC               G[AT]GC[AT]^C
             1875  GAGCTC              
BsiYI         387  CCTTTCAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1130  CCAGCTCCTGG         
BslI          387  CCTTTCAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1130  CCAGCTCCTGG         
DsaI         1416  CCGTGG               C^C[GA][CT]GG
             2277  CCATGG              
HindIII      1348  AAGCTT               A^AGCTT
             2069  AAGCTT              
NspI          252  ACATGC               [GA]CATG^[CT]
             1668  GCATGC              
SacI         1312  GAGCTC               GAGCT^C
             1875  GAGCTC              
SfcI          499  CTACAG               C^T[GA][CT]AG
             2047  CTACAG              
XmnI          870  GAAGCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1792  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com