Restriction map for
pF1KSDA1802
(Circular) (Six-base) MAPSORT of: pF1KSDA1802.seq Check: 9683 from: 1 to: 5573
pF1KSDA1802 5573 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 356 2611 356
Size: 2255 3318
Acc65I G'GTAC_C
Cuts at: 226 2589 226
Size: 2363 3210
AclI AA'CG_TT
Cuts at: 5413 5413
Size: 5573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 959 1091 2204 2228 3398 3830 4577 4845
Size: 129 132 1113 24 1170 432 747 268
Cuts at: 4845 830
Size: 1558
Fragments arranged by size:
1558 1170 1113 747 432 268 132 129
24
AflIII A'CryG_T
Cuts at: 2925 4029 5006 2925
Size: 1104 977 3492
Fragments arranged by size:
3492 1104 977
AgeI A'CCGG_T
Cuts at: 3961 3961
Size: 5573
AlwNI CAG_nnn'CTG
Cuts at: 439 700 1262 2011 2878 4445 439
Size: 261 562 749 867 1567 1567
Fragments arranged by size:
1567 1567 867 749 562 261
ApaLI G'TGCA_C
Cuts at: 4343 4343
Size: 5573
ApoI r'AATT_y
Cuts at: 241 2261 2448 2558 2577 4926 5182 241
Size: 2020 187 110 19 2349 256 632
Fragments arranged by size:
2349 2020 632 256 187 110 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 5573
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
AvaI C'yCGr_G
Cuts at: 1720 2593 1720
Size: 873 4700
AvrII C'CTAG_G
Cuts at: 1024 1084 1024
Size: 60 5513
BamHI G'GATC_C
Cuts at: 2598 2598
Size: 5573
BanI G'GyrC_C
Cuts at: 226 2589 3003 3251 3286 226
Size: 2363 414 248 35 2513
Fragments arranged by size:
2513 2363 414 248 35
BanII G_rGCy'C
Cuts at: 1442 1787 2005 2587 3617 1442
Size: 345 218 582 1030 3398
Fragments arranged by size:
3398 1030 582 345 218
BbeI G_GCGC'C
Cuts at: 3007 3255 3007
Size: 248 5325
BbsI GAAGACnn'nnnn_
Cuts at: 1214 1562 1593 1214
Size: 348 31 5194
Fragments arranged by size:
5194 348 31
BciVI GTATCCnnnnn_n'
Cuts at: 3471 4232 5573 3471
Size: 761 1341 3471
Fragments arranged by size:
3471 1341 761
BclI T'GATC_A
Cuts at: 1485 1890 2085 3093 1485
Size: 405 195 1008 3965
Fragments arranged by size:
3965 1008 405 195
BfrBI ATG'CAT
Cuts at: 382 382
Size: 5573
BglII A'GATC_T
Cuts at: 1464 3088 5125 1464
Size: 1624 2037 1912
Fragments arranged by size:
2037 1912 1624
BlpI GC'TnA_GC
Cuts at: 2012 2685 2012
Size: 673 4900
Bme1580I G_kGCm'C
Cuts at: 1773 3198 3291 4347 1773
Size: 1425 93 1056 2999
Fragments arranged by size:
2999 1425 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1360 2785 2866 3201 5345 1360
Size: 1425 81 335 2144 1588
Fragments arranged by size:
2144 1588 1425 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 5573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 607 722 842 902 1358 1418 1736 2566
Size: 71 115 120 60 456 60 318 830
Cuts at: 2566 536
Size: 3543
Fragments arranged by size:
3543 830 456 318 120 115 71 60
60
Bpu10I CC'TnA_GC
Cuts at: 620 647 953 620
Size: 27 306 5240
Fragments arranged by size:
5240 306 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1966 2749 3628 4120 4418 1966
Size: 783 879 492 298 3121
Fragments arranged by size:
3121 879 783 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1023 1603 5277 1023
Size: 580 3674 1319
Fragments arranged by size:
3674 1319 580
BsaAI yAC'GTr
Cuts at: 2565 2926 2565
Size: 361 5212
BsaBI GATnn'nnATC
Cuts at: 6 3087 5124 6
Size: 3081 2037 455
Fragments arranged by size:
3081 2037 455
BsaHI Gr'CG_yC
Cuts at: 3004 3252 3954 5450 3004
Size: 248 702 1496 3127
Fragments arranged by size:
3127 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2762 3283 3961 4235 4382 4793 2762
Size: 521 678 274 147 411 3542
Fragments arranged by size:
3542 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1298 1323 1328 1353 1725 1755 1298
Size: 25 5 25 372 30 5116
Fragments arranged by size:
5116 372 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 443 563 2227 2563 443
Size: 120 1664 336 3453
Fragments arranged by size:
3453 1664 336 120
BseYI C'CCAG_C
Cuts at: 906 1255 2998 3756 4333 4829 906
Size: 349 1743 758 577 496 1650
Fragments arranged by size:
1743 1650 758 577 496 349
BsiEI CG_ry'CG
Cuts at: 104 3115 3161 4369 104
Size: 3011 46 1208 1308
Fragments arranged by size:
3011 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1442 2005 2587 3365 3555 4347 1442
Size: 563 582 778 190 792 2668
Fragments arranged by size:
2668 792 778 582 563 190
BsmI GAATG_Cn'
Cuts at: 133 133
Size: 5573
Bsp1286I G_dGCh'C
Cuts at: 1442 1773 1787 2005 2587 3198 3291 3365 3555
Size: 331 14 218 582 611 93 74 190
Cuts at: 3555 3617 4347 1442
Size: 62 730 2668
Fragments arranged by size:
2668 730 611 582 331 218 190 93
74 62 14
BspEI T'CCGG_A
Cuts at: 4793 4793
Size: 5573
BspHI T'CATG_A
Cuts at: 4749 4749
Size: 5573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 669 762 1149 1716 2085 2623 2837 3139
Size: 153 93 387 567 369 538 214 302
Cuts at: 3139 3520 516
Size: 381 2569
Fragments arranged by size:
2569 567 538 387 381 369 302 214
153 93
BsrBI CCG'CTC
Cuts at: 50 3865 3919 5403 5568 50
Size: 3815 54 1484 165 55
Fragments arranged by size:
3815 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 177 3485 177
Size: 3308 2265
BsrFI r'CCGG_y
Cuts at: 2492 3571 3752 3961 2492
Size: 1079 181 209 4104
Fragments arranged by size:
4104 1079 209 181
BsrGI T'GTAC_A
Cuts at: 210 210
Size: 5573
BssSI C'ACGA_G
Cuts at: 3844 4202 3844
Size: 358 5215
BstAPI GCAn_nnn'nTGC
Cuts at: 2950 2950
Size: 5573
BstBI TT'CG_AA
Cuts at: 110 3934 110
Size: 3824 1749
BstEII G'GTnAC_C
Cuts at: 1457 1457
Size: 5573
BstXI CCAn_nnnn'nTGG
Cuts at: 395 893 1058 1469 395
Size: 498 165 411 4499
Fragments arranged by size:
4499 498 411 165
BstYI r'GATC_y
Cuts at: 1 435 1412 1429 1464 2598 3065 3088 3423
Size: 434 977 17 35 1134 467 23 335
Cuts at: 3423 3669 4681 4767 4779 5125 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1134 1012 977 467 449 434 346 335
246 86 35 23 17 12
BstZ17I GTA'TAC
Cuts at: 357 357
Size: 5573
Bsu36I CC'TnA_GG
Cuts at: 1452 1452
Size: 5573
BtgI C'CryG_G
Cuts at: 125 1542 2403 125
Size: 1417 861 3295
Fragments arranged by size:
3295 1417 861
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 3523 3704 3867 2068
Size: 1455 181 163 3774
Fragments arranged by size:
3774 1455 181 163
BtsI GCAGTG_nn'
Cuts at: 312 2088 2816 312
Size: 1776 728 3069
Fragments arranged by size:
3069 1776 728
ClaI AT'CG_AT
Cuts at: 1688 1688
Size: 5573
DraI TTT'AAA
Cuts at: 2572 2572
Size: 5573
DrdI GACnn_nn'nnGTC
Cuts at: 3279 4137 3279
Size: 858 4715
EaeI y'GGCC_r
Cuts at: 3158 3332 3723 3750 5095 3158
Size: 174 391 27 1345 3636
Fragments arranged by size:
3636 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3158 3158
Size: 5573
EarI CTCTTCn'nnn_
Cuts at: 461 641 2204 2498 3596 3806 461
Size: 180 1563 294 1098 210 2228
Fragments arranged by size:
2228 1563 1098 294 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3781 4091 4237 5383 3781
Size: 310 146 1146 3971
Fragments arranged by size:
3971 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1440 2003 2585 1440
Size: 563 582 4428
Fragments arranged by size:
4428 582 563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 607 722 830 842 902 959 1091 1358
Size: 71 115 108 12 60 57 132 267
Cuts at: 1358 1418 1736 2204 2228 2566 3398 3830 4577
Size: 60 318 468 24 338 832 432 747
Cuts at: 4577 4845 536
Size: 268 1264
Fragments arranged by size:
1264 832 747 468 432 338 318 268
267 132 115 108 71 60 60 57
24 12
EcoO109I rG'GnC_Cy
Cuts at: 1079 1120 1624 2712 1079
Size: 41 504 1088 3940
Fragments arranged by size:
3940 1088 504 41
EcoRI G'AATT_C
Cuts at: 2577 2577
Size: 5573
EcoRV GAT'ATC
Cuts at: 1665 1665
Size: 5573
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1590 1962 3592 1590
Size: 372 1630 3571
Fragments arranged by size:
3571 1630 372
FspI TGC'GCA
Cuts at: 3354 3354
Size: 5573
HaeII r_GCGC'y
Cuts at: 3007 3255 4277 5256 3007
Size: 248 1022 979 3324
Fragments arranged by size:
3324 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1404 1436 2092 2124 3415 3445 3447 3477 1404
Size: 32 656 32 1291 30 2 30 3500
Fragments arranged by size:
3500 1291 656 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2612 2612
Size: 5573
HindIII A'AGCT_T
Cuts at: 1474 2195 1474
Size: 721 4852
KasI G'GCGC_C
Cuts at: 3003 3251 3003
Size: 248 5325
KpnI G_GTAC'C
Cuts at: 230 2593 230
Size: 2363 3210
MluI A'CGCG_T
Cuts at: 5006 5006
Size: 5573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4244 4428 4244
Size: 184 5389
MscI TGG'CCA
Cuts at: 3334 3334
Size: 5573
MslI CAynn'nnrTG
Cuts at: 3562 3689 3562
Size: 127 5446
MspA1I CmG'CkG
Cuts at: 910 1042 1491 2684 2998 3358 4371 4616 910
Size: 132 449 1193 314 360 1013 245 1867
Fragments arranged by size:
1867 1193 1013 449 360 314 245 132
NaeI GCC'GGC
Cuts at: 2494 3754 2494
Size: 1260 4313
NarI GG'CG_CC
Cuts at: 3004 3252 3004
Size: 248 5325
NcoI C'CATG_G
Cuts at: 125 2403 125
Size: 2278 3295
NdeI CA'TA_TG
Cuts at: 221 221
Size: 5573
NgoMIV G'CCGG_C
Cuts at: 2492 3752 2492
Size: 1260 4313
NsiI A_TGCA'T
Cuts at: 384 384
Size: 5573
NspI r_CATG'y
Cuts at: 382 1798 2626 3126 4033 382
Size: 1416 828 500 907 1922
Fragments arranged by size:
1922 1416 907 828 500
PciI A'CATG_T
Cuts at: 4029 4029
Size: 5573
PflMI CCAn_nnn'nTGG
Cuts at: 1262 1262
Size: 5573
PfoI T'CCnGG_A
Cuts at: 137 824 1158 1191 1231 1261 137
Size: 687 334 33 40 30 4449
Fragments arranged by size:
4449 687 334 40 33 30
PmeI GTTT'AAAC
Cuts at: 2572 2572
Size: 5573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 4712
Size: 32 5541
PpuMI rG'GwC_Cy
Cuts at: 1079 1120 1624 1079
Size: 41 504 5028
Fragments arranged by size:
5028 504 41
PstI C_TGCA'G
Cuts at: 2620 3305 2620
Size: 685 4888
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5573
PvuII CAG'CTG
Cuts at: 910 2998 3358 910
Size: 2088 360 3125
Fragments arranged by size:
3125 2088 360
RsrII CG'GwC_CG
Cuts at: 3768 3768
Size: 5573
SacI G_AGCT'C
Cuts at: 1442 2005 2587 1442
Size: 563 582 4428
Fragments arranged by size:
4428 582 563
SalI G'TCGA_C
Cuts at: 2610 2610
Size: 5573
SanDI GG'GwC_CC
Cuts at: 1079 1079
Size: 5573
SapI GCTCTTCn'nnn_
Cuts at: 3596 3806 3596
Size: 210 5363
SbfI CC_TGCA'GG
Cuts at: 2620 2620
Size: 5573
ScaI AGT'ACT
Cuts at: 5090 5090
Size: 5573
SexAI A'CCwGG_T
Cuts at: 1620 1620
Size: 5573
SfcI C'TryA_G
Cuts at: 33 625 2173 2616 3301 4294 4485 33
Size: 592 1548 443 685 993 191 1121
Fragments arranged by size:
1548 1121 993 685 592 443 191
SfoI GGC'GCC
Cuts at: 3005 3253 3005
Size: 248 5325
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
SmaI CCC'GGG
Cuts at: 2595 2595
Size: 5573
SmlI C'TyrA_G
Cuts at: 1981 2728 3643 4135 4397 1981
Size: 747 915 492 262 3157
Fragments arranged by size:
3157 915 747 492 262
SnaBI TAC'GTA
Cuts at: 2565 2565
Size: 5573
SpeI A'CTAG_T
Cuts at: 67 2146 67
Size: 2079 3494
SphI G_CATG'C
Cuts at: 1798 2626 3126 1798
Size: 828 500 4245
Fragments arranged by size:
4245 828 500
StuI AGG'CCT
Cuts at: 1089 1089
Size: 5573
StyI C'CwwG_G
Cuts at: 125 892 958 991 1024 1084 1090 1123 1165
Size: 767 66 33 33 60 6 33 42
Cuts at: 1165 1198 2403 2707 3061 125
Size: 33 1205 304 354 2637
Fragments arranged by size:
2637 1205 767 354 304 66 60 42
33 33 33 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1269 3156 3289 3748 3957 1269
Size: 1887 133 459 209 2885
Fragments arranged by size:
2885 1887 459 209 133
TatI w'GTAC_w
Cuts at: 210 344 2030 5088 210
Size: 134 1686 3058 695
Fragments arranged by size:
3058 1686 695 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 1298 1323 1330 1355 1502 1534 5090
Size: 32 911 25 7 25 147 32 3556
Cuts at: 5090 5122 355
Size: 32 806
Fragments arranged by size:
3556 911 806 147 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 3370 3370
Size: 5573
XbaI T'CTAG_A
Cuts at: 2161 2604 2161
Size: 443 5130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3068 3068
Size: 5573
XmaI C'CCGG_G
Cuts at: 2593 2593
Size: 5573
XmnI GAAnn'nnTTC
Cuts at: 1000 1922 1000
Size: 922 4651
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BglI BmgBI BmtI
BsgI BsiWI BsmBI BssHII CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SspI SwaI XhoI ZraI