Restriction map for pF1KSDA1802
 (Linear) (Six-base) MAPSORT of: KIAA1802.seq  Check: 1927  from: 1  to: 2436



KIAA1802.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     230    2436 
   Size:        230    2206
 
Acc65I G'GTAC_C

Cuts at:      0     100    2436 
   Size:        100    2336
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     833     965    2078    2102    2436 
   Size:        704     129     132    1113      24     334

  Fragments arranged by size: 

                1113     704     334     132     129      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0     313     574    1136    1885    2436 
   Size:        313     261     562     749     551

  Fragments arranged by size: 

                 749     562     551     313     261
 
ApoI r'AATT_y

Cuts at:      0     115    2135    2322    2436 
   Size:        115    2020     187     114

  Fragments arranged by size: 

                2020     187     115     114
 
AvaI C'yCGr_G

Cuts at:      0    1594    2436 
   Size:       1594     842
 
AvrII C'CTAG_G

Cuts at:      0     898     958    2436 
   Size:        898      60    1478

  Fragments arranged by size: 

                1478     898      60
 
BanI G'GyrC_C

Cuts at:      0     100    2436 
   Size:        100    2336
 
BanII G_rGCy'C

Cuts at:      0    1316    1661    1879    2436 
   Size:       1316     345     218     557

  Fragments arranged by size: 

                1316     557     345     218
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1088    1436    1467    2436 
   Size:       1088     348      31     969

  Fragments arranged by size: 

                1088     969     348      31
 
BclI T'GATC_A

Cuts at:      0    1359    1764    1959    2436 
   Size:       1359     405     195     477

  Fragments arranged by size: 

                1359     477     405     195
 
BfrBI ATG'CAT

Cuts at:      0     256    2436 
   Size:        256    2180
 
BglII A'GATC_T

Cuts at:      0    1338    2436 
   Size:       1338    1098
 
BlpI GC'TnA_GC

Cuts at:      0    1886    2436 
   Size:       1886     550
 
Bme1580I G_kGCm'C

Cuts at:      0    1647    2436 
   Size:       1647     789
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1234    2436 
   Size:       1234    1202
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     481     596     716     776    1232    1292    1610 
   Size:        410      71     115     120      60     456      60     318

Cuts at:   1610    2436 
   Size:        826

  Fragments arranged by size: 

                 826     456     410     318     120     115      71      60
                  60
 
Bpu10I CC'TnA_GC

Cuts at:      0     494     521     827    2436 
   Size:        494      27     306    1609

  Fragments arranged by size: 

                1609     494     306      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1840    2436 
   Size:       1840     596
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     897    1477    2436 
   Size:        897     580     959

  Fragments arranged by size: 

                 959     897     580
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1172    1197    1202    1227    1599    1629    2436 
   Size:       1172      25       5      25     372      30     807

  Fragments arranged by size: 

                1172     807     372      30      25      25       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     317     437    2101    2436 
   Size:        317     120    1664     335

  Fragments arranged by size: 

                1664     335     317     120
 
BseYI C'CCAG_C

Cuts at:      0     780    1129    2436 
   Size:        780     349    1307

  Fragments arranged by size: 

                1307     780     349
 
BsiHKAI G_wGCw'C

Cuts at:      0    1316    1879    2436 
   Size:       1316     563     557

  Fragments arranged by size: 

                1316     563     557
 
BsmI GAATG_Cn'

Cuts at:      0       7    2436 
   Size:          7    2429
 
Bsp1286I G_dGCh'C

Cuts at:      0    1316    1647    1661    1879    2436 
   Size:       1316     331      14     218     557

  Fragments arranged by size: 

                1316     557     331     218      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     390     543     636    1023    1590    1959    2436 
   Size:        390     153      93     387     567     369     477

  Fragments arranged by size: 

                 567     477     390     387     369     153      93
 
BsrDI GCAATG_nn'

Cuts at:      0      51    2436 
   Size:         51    2385
 
BsrFI r'CCGG_y

Cuts at:      0    2366    2436 
   Size:       2366      70
 
BsrGI T'GTAC_A

Cuts at:      0      84    2436 
   Size:         84    2352
 
BstEII G'GTnAC_C

Cuts at:      0    1331    2436 
   Size:       1331    1105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     269     767     932    1343    2436 
   Size:        269     498     165     411    1093

  Fragments arranged by size: 

                1093     498     411     269     165
 
BstYI r'GATC_y

Cuts at:      0     309    1286    1303    1338    2436 
   Size:        309     977      17      35    1098

  Fragments arranged by size: 

                1098     977     309      35      17
 
BstZ17I GTA'TAC

Cuts at:      0     231    2436 
   Size:        231    2205
 
Bsu36I CC'TnA_GG

Cuts at:      0    1326    2436 
   Size:       1326    1110
 
BtgI C'CryG_G

Cuts at:      0    1416    2277    2436 
   Size:       1416     861     159

  Fragments arranged by size: 

                1416     861     159
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1942    2436 
   Size:       1942     494
 
BtsI GCAGTG_nn'

Cuts at:      0     186    1962    2436 
   Size:        186    1776     474

  Fragments arranged by size: 

                1776     474     186
 
ClaI AT'CG_AT

Cuts at:      0    1562    2436 
   Size:       1562     874
 
EarI CTCTTCn'nnn_

Cuts at:      0     335     515    2078    2372    2436 
   Size:        335     180    1563     294      64

  Fragments arranged by size: 

                1563     335     294     180      64
 
EcoICRI GAG'CTC

Cuts at:      0    1314    1877    2436 
   Size:       1314     563     559

  Fragments arranged by size: 

                1314     563     559
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     481     596     704     716     776     833     965 
   Size:        410      71     115     108      12      60      57     132

Cuts at:    965    1232    1292    1610    2078    2102    2436 
   Size:        267      60     318     468      24     334

  Fragments arranged by size: 

                 468     410     334     318     267     132     115     108
                  71      60      60      57      24      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     953     994    1498    2436 
   Size:        953      41     504     938

  Fragments arranged by size: 

                 953     938     504      41
 
EcoRV GAT'ATC

Cuts at:      0    1539    2436 
   Size:       1539     897
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1464    1836    2436 
   Size:       1464     372     600

  Fragments arranged by size: 

                1464     600     372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1278    1310    1966    1998    2436 
   Size:       1278      32     656      32     438

  Fragments arranged by size: 

                1278     656     438      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1348    2069    2436 
   Size:       1348     721     367

  Fragments arranged by size: 

                1348     721     367
 
KpnI G_GTAC'C

Cuts at:      0     104    2436 
   Size:        104    2332
 
MspA1I CmG'CkG

Cuts at:      0     784     916    1365    2436 
   Size:        784     132     449    1071

  Fragments arranged by size: 

                1071     784     449     132
 
NaeI GCC'GGC

Cuts at:      0    2368    2436 
   Size:       2368      68
 
NcoI C'CATG_G

Cuts at:      0    2277    2436 
   Size:       2277     159
 
NdeI CA'TA_TG

Cuts at:      0      95    2436 
   Size:         95    2341
 
NgoMIV G'CCGG_C

Cuts at:      0    2366    2436 
   Size:       2366      70
 
NsiI A_TGCA'T

Cuts at:      0     258    2436 
   Size:        258    2178
 
NspI r_CATG'y

Cuts at:      0     256    1672    2436 
   Size:        256    1416     764

  Fragments arranged by size: 

                1416     764     256
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1136    2436 
   Size:       1136    1300
 
PfoI T'CCnGG_A

Cuts at:      0      11     698    1032    1065    1105    1135    2436 
   Size:         11     687     334      33      40      30    1301

  Fragments arranged by size: 

                1301     687     334      40      33      30      11
 
PpuMI rG'GwC_Cy

Cuts at:      0     953     994    1498    2436 
   Size:        953      41     504     938

  Fragments arranged by size: 

                 953     938     504      41
 
PvuII CAG'CTG

Cuts at:      0     784    2436 
   Size:        784    1652
 
SacI G_AGCT'C

Cuts at:      0    1316    1879    2436 
   Size:       1316     563     557

  Fragments arranged by size: 

                1316     563     557
 
SanDI GG'GwC_CC

Cuts at:      0     953    2436 
   Size:        953    1483
 
SexAI A'CCwGG_T

Cuts at:      0    1494    2436 
   Size:       1494     942
 
SfcI C'TryA_G

Cuts at:      0     499    2047    2436 
   Size:        499    1548     389

  Fragments arranged by size: 

                1548     499     389
 
SmlI C'TyrA_G

Cuts at:      0    1855    2436 
   Size:       1855     581
 
SpeI A'CTAG_T

Cuts at:      0    2020    2436 
   Size:       2020     416
 
SphI G_CATG'C

Cuts at:      0    1672    2436 
   Size:       1672     764
 
StuI AGG'CCT

Cuts at:      0     963    2436 
   Size:        963    1473
 
StyI C'CwwG_G

Cuts at:      0     766     832     865     898     958     964     997    1039 
   Size:        766      66      33      33      60       6      33      42

Cuts at:   1039    1072    2277    2436 
   Size:         33    1205     159

  Fragments arranged by size: 

                1205     766     159      66      60      42      33      33
                  33      33       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1143    2436 
   Size:       1143    1293
 
TatI w'GTAC_w

Cuts at:      0      84     218    1904    2436 
   Size:         84     134    1686     532

  Fragments arranged by size: 

                1686     532     134      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     229     261    1172    1197    1204    1229    1376    1408 
   Size:        229      32     911      25       7      25     147      32

Cuts at:   1408    2436 
   Size:       1028

  Fragments arranged by size: 

                1028     911     229     147      32      32      25      25
                   7
 
XbaI T'CTAG_A

Cuts at:      0    2035    2436 
   Size:       2035     401
 
XmnI GAAnn'nnTTC

Cuts at:      0     874    1796    2436 
   Size:        874     922     640

  Fragments arranged by size: 

                 922     874     640
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AlwNI     ApoI     AvaI    AvrII     BanI
    BanII     BbsI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I  HindIII     KpnI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI    PflMI
     PfoI    PpuMI    PvuII     SacI    SanDI    SexAI     SfcI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BglI    BmgBI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI   BstAPI
    BstBI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     MslI     NarI     NheI
     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     SspI     SwaI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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