Restriction map for pF1KSDA1785
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       FauI        
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1496  GGTACC               G^GTACC
AccI          766  GTAGAC               GT^[AC][GT]AC
AflII        1417  CTTAAG               C^TTAAG
AflIII       1483  ACATGT               A^C[GA][CT]GT
Alw44I       1652  GTGCAC               G^TGCAC
ApaLI        1652  GTGCAC               G^TGCAC
Asp718I      1496  GGTACC               G^GTACC
AspHI        1652  GTGCAC               G[AT]GC[AT]^C
AvaI           32  CTCGGG               C^[CT]CG[GA]G
BalI          378  TGGCCA               TGG^CCA
BamHI        1116  GGATCC               G^GATCC
BfrI         1417  CTTAAG               C^TTAAG
BglII         780  AGATCT               A^GATCT
BsaAI         450  TACGTG               [CT]AC^GT[GA]
BsiHKAI      1652  GTGCAC               G[AT]GC[AT]^C
BsmFI        1589  GTCCC                GGGAC(10/14)
BsmI          956  GAATGC               GAATGC(1/-1)
BsoBI          32  CTCGGG               C^[CT]CG[GA]G
BspLU11I     1483  ACATGT               A^CATGT
BssKI         146  CCAGG                ^CC[ACGT]GG
BssSI        1772  CTCGTG               CACGAG(-5/-1)
BstAPI       1238  GCATACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         146  CCAGG                CC^[AT]GG
BstXI          59  CCAAATTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1034  GCGC                 GCG^C
DraII        1319  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     1319  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         172  GAATTC               G^AATTC
EcoRII        146  CCAGG                ^CC[AT]GG
EcoT22I      1068  ATGCAT               ATGCA^T
HaeII        1033  GGCGCT               [GA]GCGC^[CT]
HapII          48  CCGG                 C^CGG
HgaI         1253  GCGTC                GACGC(5/10)
HhaI         1034  GCGC                 GCG^C
HinP1I       1034  GCGC                 G^CGC
HincII        325  GTCAAC               GT[CT]^[GA]AC
HindII        325  GTCAAC               GT[CT]^[GA]AC
HpaII          48  CCGG                 C^CGG
HphI           56  TCACC                GGTGA(8/7)
KpnI         1496  GGTACC               GGTAC^C
MluNI         378  TGGCCA               TGG^CCA
MscI          378  TGGCCA               TGG^CCA
MspA1I       1295  CAGCTG               C[AC]G^C[GT]G
MspI           48  CCGG                 C^CGG
MvaI          146  CCAGG                CC^[AT]GG
NsiI         1068  ATGCAT               ATGCA^T
NspI         1483  ACATGT               [GA]CATG^[CT]
PshAI        1582  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          272  CTGCAG               CTGCA^G
PvuII        1295  CAGCTG               CAG^CTG
SapI          916  GAAGAGC              GCTCTTC(1/4)
ScrFI         146  CCAGG                CC^[ACGT]GG
SmlI         1417  CTTAAG               C^T[CT][GA]AG
StuI         1197  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         569  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1046  CAGACTCTG           
AseI          733  ATTAAT               AT^TAAT
              969  ATTAAT              
AsnI          733  ATTAAT               AT^TAAT
              969  ATTAAT              
Asp700I       979  GAACGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1440  GAATAACTTC          
AvaII         294  GGTCC                G^G[AT]CC
              991  GGTCC               
BanI          155  GGCACC               G^G[CT][GA]CC
             1496  GGTACC              
BanII         213  GGGCTC               G[GA]GC[CT]^C
              248  GGGCTC              
BsaJI         896  CCAAGG               C^C[ACGT][ACGT]GG
             1429  CCAAGG              
BseRI         353  CTCCTC               GAGGAG(10/8)
               85  GAGGAG              
BsgI          566  GTGCAG               GTGCAG(16/14)
              911  GTGCAG              
BsmAI        1571  GAGAC                GTCTC(1/5)
             1828  GAGAC               
BspHI         590  TCATGA               T^CATGA
             1628  TCATGA              
EaeI          140  TGGCCG               [CT]^GGCC[GA]
              378  TGGCCA              
EcoT14I       896  CCAAGG               C^C[AT][AT]GG
             1429  CCAAGG              
PshBI         733  ATTAAT               AT^TAAT
              969  ATTAAT              
RcaI          590  TCATGA               T^CATGA
             1628  TCATGA              
SfcI          272  CTGCAG               C^T[GA][CT]AG
             1180  CTACAG              
SspI          682  AATATT               AAT^ATT
              844  AATATT              
StyI          896  CCAAGG               C^C[AT][AT]GG
             1429  CCAAGG              
TaqI          360  TCGA                 T^CGA
              374  TCGA                
TthHB8I       360  TCGA                 T^CGA
              374  TCGA                
XbaI          741  TCTAGA               T^CTAGA
             1374  TCTAGA              
XmnI          979  GAACGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1440  GAATAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BfaI        BmyI        BsiYI       
BslI        Bsp1286I    BsrDI       BsrI        BstF5I      
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        Fnu4HI      FokI        HaeIII      HinfI       
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com