Restriction map for pF1KSDA1785
 (Linear) (Six-base) MAPSORT of: KIAA1785.seq  Check: 4726  from: 1  to: 1851



KIAA1785.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     767    1851 
   Size:        767    1084
 
Acc65I G'GTAC_C

Cuts at:      0    1496    1851 
   Size:       1496     355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    1431    1851 
   Size:        307    1124     420

  Fragments arranged by size: 

                1124     420     307
 
AflII C'TTAA_G

Cuts at:      0    1417    1851 
   Size:       1417     434
 
AflIII A'CryG_T

Cuts at:      0    1483    1851 
   Size:       1483     368
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     209     581    1619    1781    1851 
   Size:        209     372    1038     162      70

  Fragments arranged by size: 

                1038     372     209     162      70
 
AlwNI CAG_nnn'CTG

Cuts at:      0     574    1051    1851 
   Size:        574     477     800

  Fragments arranged by size: 

                 800     574     477
 
ApaLI G'TGCA_C

Cuts at:      0    1652    1851 
   Size:       1652     199
 
ApoI r'AATT_y

Cuts at:      0     172    1056    1510    1725    1851 
   Size:        172     884     454     215     126

  Fragments arranged by size: 

                 884     454     215     172     126
 
AseI AT'TA_AT

Cuts at:      0     734     970    1851 
   Size:        734     236     881

  Fragments arranged by size: 

                 881     734     236
 
AvaI C'yCGr_G

Cuts at:      0      32    1851 
   Size:         32    1819
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1199    1232    1851 
   Size:       1199      33     619

  Fragments arranged by size: 

                1199     619      33
 
BamHI G'GATC_C

Cuts at:      0    1116    1851 
   Size:       1116     735
 
BanI G'GyrC_C

Cuts at:      0     155    1496    1851 
   Size:        155    1341     355

  Fragments arranged by size: 

                1341     355     155
 
BanII G_rGCy'C

Cuts at:      0     217     252    1851 
   Size:        217      35    1599

  Fragments arranged by size: 

                1599     217      35
 
BfrBI ATG'CAT

Cuts at:      0    1070    1851 
   Size:       1070     781
 
BglII A'GATC_T

Cuts at:      0     780    1851 
   Size:        780    1071
 
Bme1580I G_kGCm'C

Cuts at:      0     158    1656    1851 
   Size:        158    1498     195

  Fragments arranged by size: 

                1498     195     158
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     111    1851 
   Size:        111    1740
 
BsaAI yAC'GTr

Cuts at:      0     452    1851 
   Size:        452    1399
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     308     338    1851 
   Size:        308      30    1513

  Fragments arranged by size: 

                1513     308      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     344    1851 
   Size:        100     244    1507

  Fragments arranged by size: 

                1507     244     100
 
BseYI C'CCAG_C

Cuts at:      0    1203    1851 
   Size:       1203     648
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     932    1851 
   Size:        587     345     919

  Fragments arranged by size: 

                 919     587     345
 
BsiHKAI G_wGCw'C

Cuts at:      0    1656    1851 
   Size:       1656     195
 
BsmI GAATG_Cn'

Cuts at:      0     962    1851 
   Size:        962     889
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     217     252    1656    1851 
   Size:        158      59      35    1404     195

  Fragments arranged by size: 

                1404     195     158      59      35
 
BspHI T'CATG_A

Cuts at:      0     590    1628    1851 
   Size:        590    1038     223

  Fragments arranged by size: 

                1038     590     223
 
BsrDI GCAATG_nn'

Cuts at:      0     193     553     821    1753    1851 
   Size:        193     360     268     932      98

  Fragments arranged by size: 

                 932     360     268     193      98
 
BssSI C'ACGA_G

Cuts at:      0    1772    1851 
   Size:       1772      79
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1244    1851 
   Size:       1244     607
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      66    1851 
   Size:         66    1785
 
BstYI r'GATC_y

Cuts at:      0       3     780    1116    1851 
   Size:          3     777     336     735

  Fragments arranged by size: 

                 777     735     336       3
 
BtsI GCAGTG_nn'

Cuts at:      0     198    1765    1851 
   Size:        198    1567      86

  Fragments arranged by size: 

                1567     198      86
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     668     703    1851 
   Size:        668      35    1148

  Fragments arranged by size: 

                1148     668      35
 
EaeI y'GGCC_r

Cuts at:      0     140     378    1851 
   Size:        140     238    1473

  Fragments arranged by size: 

                1473     238     140
 
EarI CTCTTCn'nnn_

Cuts at:      0     362     911    1460    1851 
   Size:        362     549     549     391

  Fragments arranged by size: 

                 549     549     391     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    1431    1851 
   Size:        307    1124     420

  Fragments arranged by size: 

                1124     420     307
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1320    1851 
   Size:       1320     531
 
EcoRI G'AATT_C

Cuts at:      0     172    1851 
   Size:        172    1679
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1461    1851 
   Size:       1461     390
 
HaeII r_GCGC'y

Cuts at:      0    1037    1851 
   Size:       1037     814
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     308     340    1022    1054    1851 
   Size:        308      32     682      32     797

  Fragments arranged by size: 

                 797     682     308      32      32
 
HincII GTy'rAC

Cuts at:      0     327    1851 
   Size:        327    1524
 
KpnI G_GTAC'C

Cuts at:      0    1500    1851 
   Size:       1500     351
 
MscI TGG'CCA

Cuts at:      0     380    1851 
   Size:        380    1471
 
MslI CAynn'nnrTG

Cuts at:      0     563     595    1661    1758    1851 
   Size:        563      32    1066      97      93

  Fragments arranged by size: 

                1066     563      97      93      32
 
MspA1I CmG'CkG

Cuts at:      0    1297    1851 
   Size:       1297     554
 
NsiI A_TGCA'T

Cuts at:      0    1072    1851 
   Size:       1072     779
 
NspI r_CATG'y

Cuts at:      0    1487    1851 
   Size:       1487     364
 
PciI A'CATG_T

Cuts at:      0    1483    1851 
   Size:       1483     368
 
PshAI GACnn'nnGTC

Cuts at:      0    1586    1851 
   Size:       1586     265
 
PsiI TTA'TAA

Cuts at:      0    1802    1851 
   Size:       1802      49
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     811     843    1851 
   Size:        811      32    1008

  Fragments arranged by size: 

                1008     811      32
 
PstI C_TGCA'G

Cuts at:      0     276    1851 
   Size:        276    1575
 
PvuII CAG'CTG

Cuts at:      0    1297    1851 
   Size:       1297     554
 
SapI GCTCTTCn'nnn_

Cuts at:      0     911    1851 
   Size:        911     940
 
SfcI C'TryA_G

Cuts at:      0     272    1180    1851 
   Size:        272     908     671

  Fragments arranged by size: 

                 908     671     272
 
SmlI C'TyrA_G

Cuts at:      0    1417    1851 
   Size:       1417     434
 
SspI AAT'ATT

Cuts at:      0     684     846    1851 
   Size:        684     162    1005

  Fragments arranged by size: 

                1005     684     162
 
StuI AGG'CCT

Cuts at:      0    1199    1851 
   Size:       1199     652
 
StyI C'CwwG_G

Cuts at:      0     896    1429    1851 
   Size:        896     533     422

  Fragments arranged by size: 

                 896     533     422
 
TatI w'GTAC_w

Cuts at:      0    1369    1851 
   Size:       1369     482
 
XbaI T'CTAG_A

Cuts at:      0     741    1374    1851 
   Size:        741     633     477

  Fragments arranged by size: 

                 741     633     477
 
XmnI GAAnn'nnTTC

Cuts at:      0     983    1444    1851 
   Size:        983     461     407

  Fragments arranged by size: 

                 983     461     407
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AlfI    AlwNI    ApaLI
     ApoI     AseI     AvaI     BaeI    BamHI     BanI    BanII    BfrBI
    BglII Bme1580I     BplI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BssSI   BstAPI    BstXI
    BstYI     BtsI    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII     KpnI     MscI     MslI   MspA1I
     NsiI     NspI     PciI    PshAI     PsiI     PsrI     PstI    PvuII
     SapI     SfcI     SmlI     SspI     StuI     StyI     TatI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AhdI     AleI     AloI
     ApaI     AscI    AsiSI    AvrII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI     BlpI    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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