Restriction map for pF1KA1780
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BalI        
BlnI        BseAI       BsiEI       Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HpaI        KpnI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflII        2523  CTTAAG               C^TTAAG
AocI         1066  CCTGAGG              CC^T[ACGT]AGG
AspI          610  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          805  CCCGAG               C^[CT]CG[GA]G
BamHI        3288  GGATCC               G^GATCC
BbeI         1957  GGCGCC               GGCGC^C
BbvCI        1717  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2973  CGAGCTCGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2523  CTTAAG               C^TTAAG
BlpI         1615  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1615  GCTGAGC              GC^T[ACGT]AGC
BsaBI         418  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          964  GAGACC               GGTCTC(1/5)
BsaWI         649  ACCGGA               [AT]^CCGG[AT]
BseRI         728  GAGGAG               GAGGAG(10/8)
BsiWI        1598  CGTACG               C^GTACG
BsmBI        3277  CGTCTC               CGTCTC(1/5)
BsoBI         805  CCCGAG               C^[CT]CG[GA]G
BspLU11I     1195  ACATGT               A^CATGT
BsrBI        1005  GAGCGG               CCGCTC(-3/-3)
BssSI         171  CACGAG               CACGAG(-5/-1)
Bsu36I       1066  CCTGAGG              CC^T[ACGT]AGG
CelII        1615  GCTGAGC              GC^T[ACGT]AGC
DraI          199  TTTAAA               TTT^AAA
Eco81I       1066  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3036  GAATTC               G^AATTC
HindIII      1220  AAGCTT               A^AGCTT
KasI         1957  GGCGCC               G^GCGCC
MamI          418  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1957  GGCGCC               GG^CGCC
NcoI          342  CCATGG               C^CATGG
PflFI         610  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        1239  CAGCTG               CAG^CTG
SacI         2974  GAGCTC               GAGCT^C
ScaI         2536  AGTACT               AGT^ACT
SfoI         1957  GGCGCC               GGC^GCC
SmlI         2523  CTTAAG               C^T[CT][GA]AG
TaqI          240  TCGA                 T^CGA
Tth111I       610  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       240  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         209  CGCG                 CG^CG
             1561  CGCG                
AcyI         1957  GGCGCC               G[GA]^CG[CT]C
             3275  GGCGTC              
Aor51HI      1043  AGCGCT               AGC^GCT
             1619  AGCGCT              
Asp700I      3032  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3167  GAAGAGATTC          
BbsI           85  GAAGAC               GAAGAC(2/6)
              252  GAAGAC              
BciVI         643  GGATAC               GTATCC(6/5)
              901  GGATAC              
BfaI         2242  CTAG                 C^TAG
             2599  CTAG                
BglI          764  GCCCTTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1586  GCCAGGATGGC         
BglII        1232  AGATCT               A^GATCT
             2056  AGATCT              
BpuAI          85  GAAGAC               GAAGAC(2/6)
              252  GAAGAC              
BsaHI        1957  GGCGCC               G[GA]^CG[CT]C
             3275  GGCGTC              
BstAPI       1027  GCAGGCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2087  GCAGTGACTGC         
BstUI         209  CGCG                 CG^CG
             1561  CGCG                
DsaI          342  CCATGG               C^C[GA][CT]GG
             1658  CCACGG              
Eco47III     1043  AGCGCT               AGC^GCT
             1619  AGCGCT              
EcoT14I       342  CCATGG               C^C[AT][AT]GG
             3252  CCAAGG              
HgaI         1724  GACGC                GACGC(5/10)
             3276  GCGTC               
Hin1I        1957  GGCGCC               G[GA]^CG[CT]C
             3275  GGCGTC              
HincII        979  GTCAAC               GT[CT]^[GA]AC
             2824  GTCAAC              
HindII        979  GTCAAC               GT[CT]^[GA]AC
             2824  GTCAAC              
MaeI         2242  CTAG                 C^TAG
             2599  CTAG                
MvnI          209  CGCG                 CG^CG
             1561  CGCG                
NaeI         2940  GCCGGC               GCC^GGC
             3159  GCCGGC              
NgoMIV       2940  GCCGGC               G^CCGGC
             3159  GCCGGC              
SphI         1012  GCATGC               GCATG^C
             2666  GCATGC              
StyI          342  CCATGG               C^C[AT][AT]GG
             3252  CCAAGG              
XmnI         3032  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3167  GAAGAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
ApoI        AspHI       AvaII       BanI        BanII       
BbrPI       BbvI        BclI        BcnI        BmyI        
BpmI        BsaAI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      EarI        EcoO109I    
EcoRII      FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PmaCI       
PmlI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XcmI        XhoII       
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