Restriction map for pF1KA1780
 (Linear) (Six-base) MAPSORT of: KIAA1780.seq  Check: 4393  from: 1  to: 3420



KIAA1780.orf

 With 182 enzymes: SgfI * 

                        May 30, 2008 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     613    2121    2434    3420 
   Size:        613    1508     313     986

  Fragments arranged by size: 

                1508     986     613     313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2354    3188    3420 
   Size:       2354     834     232

  Fragments arranged by size: 

                2354     834     232
 
AfeI AGC'GCT

Cuts at:      0    1045    1621    3420 
   Size:       1045     576    1799

  Fragments arranged by size: 

                1799    1045     576
 
AflII C'TTAA_G

Cuts at:      0    2523    3420 
   Size:       2523     897
 
AflIII A'CryG_T

Cuts at:      0     862    1195    1543    2141    3420 
   Size:        862     333     348     598    1279

  Fragments arranged by size: 

                1279     862     598     348     333
 
AleI CACnn'nnGTG

Cuts at:      0    1059    3420 
   Size:       1059    2361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1662    2289    3420 
   Size:       1662     627    1131

  Fragments arranged by size: 

                1662    1131     627
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1338    1370    3420 
   Size:       1338      32    2050

  Fragments arranged by size: 

                2050    1338      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     127     413    2093    3420 
   Size:        127     286    1680    1327

  Fragments arranged by size: 

                1680    1327     286     127
 
ApaI G_GGCC'C

Cuts at:      0    1735    1910    2128    3420 
   Size:       1735     175     218    1292

  Fragments arranged by size: 

                1735    1292     218     175
 
ApaLI G'TGCA_C

Cuts at:      0    1281    1547    1805    2264    3420 
   Size:       1281     266     258     459    1156

  Fragments arranged by size: 

                1281    1156     459     266     258
 
ApoI r'AATT_y

Cuts at:      0     161    2012    3036    3420 
   Size:        161    1851    1024     384

  Fragments arranged by size: 

                1851    1024     384     161
 
AvaI C'yCGr_G

Cuts at:      0     805    3420 
   Size:        805    2615
 
BamHI G'GATC_C

Cuts at:      0    3288    3420 
   Size:       3288     132
 
BanI G'GyrC_C

Cuts at:      0     452     553     917    1822    1957    3420 
   Size:        452     101     364     905     135    1463

  Fragments arranged by size: 

                1463     905     452     364     135     101
 
BanII G_rGCy'C

Cuts at:      0     474    1076    1735    1910    2128    2219    2978    3420 
   Size:        474     602     659     175     218      91     759     442

  Fragments arranged by size: 

                 759     659     602     474     442     218     175      91
            
 
BbeI G_GCGC'C

Cuts at:      0    1961    3420 
   Size:       1961    1459
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     259    3420 
   Size:         92     167    3161

  Fragments arranged by size: 

                3161     167      92
 
BbvCI CC'TCA_GC

Cuts at:      0    1718    3420 
   Size:       1718    1702
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2962    2996    3420 
   Size:       2962      34     424

  Fragments arranged by size: 

                2962     424      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     637     895    3420 
   Size:        637     258    2525

  Fragments arranged by size: 

                2525     637     258
 
BclI T'GATC_A

Cuts at:      0     156     423    2484    3420 
   Size:        156     267    2061     936

  Fragments arranged by size: 

                2061     936     267     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0     770    1592    3420 
   Size:        770     822    1828

  Fragments arranged by size: 

                1828     822     770
 
BglII A'GATC_T

Cuts at:      0    1232    2056    3420 
   Size:       1232     824    1364

  Fragments arranged by size: 

                1364    1232     824
 
BlpI GC'TnA_GC

Cuts at:      0    1616    3420 
   Size:       1616    1804
 
Bme1580I G_kGCm'C

Cuts at:      0     556     784    1285    1293    1551    1735    1809    1910 
   Size:        556     228     501       8     258     184      74     101

Cuts at:   1910    2128    2268    2368    3420 
   Size:        218     140     100    1052

  Fragments arranged by size: 

                1052     556     501     258     228     218     184     140
                 101     100      74       8
 
BmgBI CAC'GTC

Cuts at:      0     615     744     863    2823    3420 
   Size:        615     129     119    1960     597

  Fragments arranged by size: 

                1960     615     597     129     119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     117     438    1454    1976    2130    2450    2777    2796 
   Size:        117     321    1016     522     154     320     327      19

Cuts at:   2796    2939    3420 
   Size:        143     481

  Fragments arranged by size: 

                1016     522     481     327     321     320     154     143
                 117      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2255    3420 
   Size:       2255    1165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     770    1166    3420 
   Size:        390     380     396    2254

  Fragments arranged by size: 

                2254     396     390     380
 
Bpu10I CC'TnA_GC

Cuts at:      0      25    1718    3067    3368    3420 
   Size:         25    1693    1349     301      52

  Fragments arranged by size: 

                1693    1349     301      52      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     958    3420 
   Size:        958    2462
 
BsaAI yAC'GTr

Cuts at:      0    1002    1544    1598    2144    3420 
   Size:       1002     542      54     546    1276

  Fragments arranged by size: 

                1276    1002     546     542      54
 
BsaBI GATnn'nnATC

Cuts at:      0     422    3420 
   Size:        422    2998
 
BsaHI Gr'CG_yC

Cuts at:      0    1958    3276    3420 
   Size:       1958    1318     144

  Fragments arranged by size: 

                1958    1318     144
 
BsaWI w'CCGG_w

Cuts at:      0     649    3420 
   Size:        649    2771
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1185    1215    3420 
   Size:       1185      30    2205

  Fragments arranged by size: 

                2205    1185      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     743    3420 
   Size:        743    2677
 
BseYI C'CCAG_C

Cuts at:      0     386    1727    3420 
   Size:        386    1341    1693

  Fragments arranged by size: 

                1693    1341     386
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     426    1524    2007    2027    2208    2910    3420 
   Size:        162     264    1098     483      20     181     702     510

  Fragments arranged by size: 

                1098     702     510     483     264     181     162      20
            
 
BsiHKAI G_wGCw'C

Cuts at:      0    1285    1551    1809    2268    2978    3420 
   Size:       1285     266     258     459     710     442

  Fragments arranged by size: 

                1285     710     459     442     266     258
 
BsiWI C'GTAC_G

Cuts at:      0    1598    3420 
   Size:       1598    1822
 
BsmI GAATG_Cn'

Cuts at:      0     631     760     844    3420 
   Size:        631     129      84    2576

  Fragments arranged by size: 

                2576     631     129      84
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3283    3420 
   Size:       3283     137
 
Bsp1286I G_dGCh'C

Cuts at:      0     474     556     784    1076    1285    1293    1551    1735 
   Size:        474      82     228     292     209       8     258     184

Cuts at:   1735    1809    1910    2128    2219    2268    2368    2978    3420 
   Size:         74     101     218      91      49     100     610     442

  Fragments arranged by size: 

                 610     474     442     292     258     228     218     209
                 184     101     100      91      82      74      49       8
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     613     976    1546    2029    2121    2434    3344    3420 
   Size:        613     363     570     483      92     313     910      76

  Fragments arranged by size: 

                 910     613     570     483     363     313      92      76
            
 
BsrBI CCG'CTC

Cuts at:      0    1007    3420 
   Size:       1007    2413
 
BsrDI GCAATG_nn'

Cuts at:      0     888    1665    1974    2573    2751    2935    3420 
   Size:        888     777     309     599     178     184     485

  Fragments arranged by size: 

                 888     777     599     485     309     184     178
 
BsrFI r'CCGG_y

Cuts at:      0     449    1924    2940    3159    3420 
   Size:        449    1475    1016     219     261

  Fragments arranged by size: 

                1475    1016     449     261     219
 
BssSI C'ACGA_G

Cuts at:      0     171    3420 
   Size:        171    3249
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1033    2093    3420 
   Size:       1033    1060    1327

  Fragments arranged by size: 

                1327    1060    1033
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      58    1642    2311    2440    3420 
   Size:         58    1584     669     129     980

  Fragments arranged by size: 

                1584     980     669     129      58
 
BstYI r'GATC_y

Cuts at:      0     666    1232    1838    2056    3288    3420 
   Size:        666     566     606     218    1232     132

  Fragments arranged by size: 

                1232     666     606     566     218     132
 
Bsu36I CC'TnA_GG

Cuts at:      0    1067    3420 
   Size:       1067    2353
 
BtgI C'CryG_G

Cuts at:      0     342    1658    3420 
   Size:        342    1316    1762

  Fragments arranged by size: 

                1762    1316     342
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     431    1849    3420 
   Size:        431    1418    1571

  Fragments arranged by size: 

                1571    1418     431
 
BtsI GCAGTG_nn'

Cuts at:      0     438    2094    2323    3420 
   Size:        438    1656     229    1097

  Fragments arranged by size: 

                1656    1097     438     229
 
DraI TTT'AAA

Cuts at:      0     201    3420 
   Size:        201    3219
 
DraIII CAC_nnn'GTG

Cuts at:      0     700     788    1437    2804    3420 
   Size:        700      88     649    1367     616

  Fragments arranged by size: 

                1367     700     649     616      88
 
EarI CTCTTCn'nnn_

Cuts at:      0    2614    2899    3162    3420 
   Size:       2614     285     263     258

  Fragments arranged by size: 

                2614     285     263     258
 
EcoICRI GAG'CTC

Cuts at:      0    2976    3420 
   Size:       2976     444
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     770    1166    2354    3188    3420 
   Size:        390     380     396    1188     834     232

  Fragments arranged by size: 

                1188     834     396     390     380     232
 
EcoO109I rG'GnC_Cy

Cuts at:      0     433     941    1535    1731    1732    1907    2124    2444 
   Size:        433     508     594     196       1     175     217     320

Cuts at:   2444    2911    3420 
   Size:        467     509

  Fragments arranged by size: 

                 594     509     508     467     433     320     217     196
                 175       1
 
EcoRI G'AATT_C

Cuts at:      0    3036    3420 
   Size:       3036     384
 
HaeII r_GCGC'y

Cuts at:      0     531    1047    1623    1961    2177    3420 
   Size:        531     516     576     338     216    1243

  Fragments arranged by size: 

                1243     576     531     516     338     216
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     222     254    1185    1217    2469    2501    3139    3171 
   Size:        222      32     931      32    1252      32     638      32

Cuts at:   3171    3343    3375    3420 
   Size:        172      32      45

  Fragments arranged by size: 

                1252     931     638     222     172      45      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     981    2826    3420 
   Size:        981    1845     594

  Fragments arranged by size: 

                1845     981     594
 
HindIII A'AGCT_T

Cuts at:      0    1220    3420 
   Size:       1220    2200
 
KasI G'GCGC_C

Cuts at:      0    1957    3420 
   Size:       1957    1463
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2059    2487    3420 
   Size:       2059     428     933

  Fragments arranged by size: 

                2059     933     428
 
MslI CAynn'nnrTG

Cuts at:      0    1059    1497    1554    1640    2271    2744    3420 
   Size:       1059     438      57      86     631     473     676

  Fragments arranged by size: 

                1059     676     631     473     438      86      57
 
MspA1I CmG'CkG

Cuts at:      0     542     632    1241    1673    1904    3420 
   Size:        542      90     609     432     231    1516

  Fragments arranged by size: 

                1516     609     542     432     231      90
 
NaeI GCC'GGC

Cuts at:      0    2942    3161    3420 
   Size:       2942     219     259

  Fragments arranged by size: 

                2942     259     219
 
NarI GG'CG_CC

Cuts at:      0    1958    3420 
   Size:       1958    1462
 
NcoI C'CATG_G

Cuts at:      0     342    3420 
   Size:        342    3078
 
NdeI CA'TA_TG

Cuts at:      0    2379    3087    3180    3294    3420 
   Size:       2379     708      93     114     126

  Fragments arranged by size: 

                2379     708     126     114      93
 
NgoMIV G'CCGG_C

Cuts at:      0    2940    3159    3420 
   Size:       2940     219     261

  Fragments arranged by size: 

                2940     261     219
 
NspI r_CATG'y

Cuts at:      0    1016    1199    1496    1883    2670    3420 
   Size:       1016     183     297     387     787     750

  Fragments arranged by size: 

                1016     787     750     387     297     183
 
PasI CC'CwG_GG

Cuts at:      0    2899    3420 
   Size:       2899     521
 
PciI A'CATG_T

Cuts at:      0    1195    3420 
   Size:       1195    2225
 
PfoI T'CCnGG_A

Cuts at:      0     285     897    1203    3420 
   Size:        285     612     306    2217

  Fragments arranged by size: 

                2217     612     306     285
 
PmlI CAC'GTG

Cuts at:      0    1002    1544    2144    3420 
   Size:       1002     542     600    1276

  Fragments arranged by size: 

                1276    1002     600     542
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2721    2753    3420 
   Size:       2721      32     667

  Fragments arranged by size: 

                2721     667      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     433     941    1535    2444    3420 
   Size:        433     508     594     909     976

  Fragments arranged by size: 

                 976     909     594     508     433
 
PspOMI G'GGCC_C

Cuts at:      0    1731    1906    2124    3420 
   Size:       1731     175     218    1296

  Fragments arranged by size: 

                1731    1296     218     175
 
PstI C_TGCA'G

Cuts at:      0    1240    1633    1873    2173    2458    3420 
   Size:       1240     393     240     300     285     962

  Fragments arranged by size: 

                1240     962     393     300     285     240
 
PvuII CAG'CTG

Cuts at:      0    1241    3420 
   Size:       1241    2179
 
SacI G_AGCT'C

Cuts at:      0    2978    3420 
   Size:       2978     442
 
SanDI GG'GwC_CC

Cuts at:      0     433    3420 
   Size:        433    2987
 
ScaI AGT'ACT

Cuts at:      0    2538    3420 
   Size:       2538     882
 
SfcI C'TryA_G

Cuts at:      0    1236    1629    1869    2169    2454    3236    3420 
   Size:       1236     393     240     300     285     782     184

  Fragments arranged by size: 

                1236     782     393     300     285     240     184
 
SfoI GGC'GCC

Cuts at:      0    1959    3420 
   Size:       1959    1461
 
SmlI C'TyrA_G

Cuts at:      0    2523    3420 
   Size:       2523     897
 
SphI G_CATG'C

Cuts at:      0    1016    2670    3420 
   Size:       1016    1654     750

  Fragments arranged by size: 

                1654    1016     750
 
StyI C'CwwG_G

Cuts at:      0     342    3252    3420 
   Size:        342    2910     168

  Fragments arranged by size: 

                2910     342     168
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2815    3420 
   Size:       2815     605
 
TatI w'GTAC_w

Cuts at:      0    1411    2320    2536    3111    3420 
   Size:       1411     909     216     575     309

  Fragments arranged by size: 

                1411     909     575     309     216
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1799    1831    1989    2021    2073    2105    3420 
   Size:       1799      32     158      32      52      32    1315

  Fragments arranged by size: 

                1799    1315     158      52      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     613    3420 
   Size:        613    2807
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     463     592     850    2117    3420 
   Size:        463     129     258    1267    1303

  Fragments arranged by size: 

                1303    1267     463     258     129
 
XmnI GAAnn'nnTTC

Cuts at:      0    3036    3171    3420 
   Size:       3036     135     249

  Fragments arranged by size: 

                3036     249     135
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AflII   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SanDI     ScaI     SfcI     SfoI     SmlI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AgeI     AhdI     AscI     AseI
    AsiSI    AvrII     BaeI    BfrBI     BmtI    BpuEI    BsiEI    BspEI
    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DrdI     EaeI     EagI     EciI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KpnI     MfeI     MluI     MscI     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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