Restriction map for pF1KSDA1737
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# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BamHI       
BanI        BbeI        BbvCI       BcgI        BcnI        
BfrI        BglII       BlnI        BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          471  GAATTC               [GA]^AATT[CT]
AcyI          639  GACGCC               G[GA]^CG[CT]C
ApaI         1100  GGGCCC               GGGCC^C
ApoI          471  GAATTC               [GA]^AATT[CT]
Asp700I       249  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII        1145  GGTCC                G^G[AT]CC
AviII         799  TGCGCA               TGC^GCA
BbrPI         748  CACGTG               CAC^GTG
BciVI         736  GTATCC               GTATCC(6/5)
BclI         1182  TGATCA               T^GATCA
BglI         1102  GCCCCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          189  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      189  GCTGAGC              GC^T[ACGT]AGC
BsaAI         748  CACGTG               [CT]AC^GT[GA]
BsaHI         639  GACGCC               G[GA]^CG[CT]C
BsgI          550  GTGCAG               GTGCAG(16/14)
BspHI         281  TCATGA               T^CATGA
BspMI         706  GCAGGT               ACCTGC(4/8)
BsrFI         732  GCCGGT               [GA]^CCGG[CT]
CelII         189  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        732  GCCGGT               [GA]^CCGG[CT]
EarI          725  CTCTTC               CTCTTC(1/4)
EcoRI         471  GAATTC               G^AATTC
EcoRV         910  GATATC               GAT^ATC
FauI          784  CCCGC                CCCGC(4/6)
FbaI         1182  TGATCA               T^GATCA
FspI          799  TGCGCA               TGC^GCA
HapII         733  CCGG                 C^CGG
HgaI          639  GACGC                GACGC(5/10)
Hin1I         639  GACGCC               G[GA]^CG[CT]C
HpaII         733  CCGG                 C^CGG
Ksp632I       725  CTCTTC               CTCTTC(1/4)
MaeII         749  ACGT                 A^CGT
MseI         1135  TTAA                 T^TAA
MspI          733  CCGG                 C^CGG
NspI          185  ACATGC               [GA]CATG^[CT]
PmaCI         748  CACGTG               CAC^GTG
PmlI          748  CACGTG               CAC^GTG
PvuII        1072  CAGCTG               CAG^CTG
RcaI          281  TCATGA               T^CATGA
TaqI          637  TCGA                 T^CGA
Tru9I        1135  TTAA                 T^TAA
TthHB8I       637  TCGA                 T^CGA
XcmI          740  CCAGCAGTCACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          249  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          400  GTAC                 GT^AC
              566  GTAC                
AocI          197  CCTTAGG              CC^T[ACGT]AGG
             1105  CCTCAGG             
BsmAI         573  GAGAC                GTCTC(1/5)
              267  GTCTC               
BsmFI         622  GTCCC                GGGAC(10/14)
              632  GTCCC               
BsmI          249  GAATGC               GAATGC(1/-1)
             1174  GCATTC              
BstAPI        244  GCAAAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              684  GCAGGGTGTGC         
Bsu36I        197  CCTTAGG              CC^T[ACGT]AGG
             1105  CCTCAGG             
Cac8I         642  GCCAGC               GC[ACGT]^[ACGT]GC
             1125  GCAGGC              
DsaI          272  CCATGG               C^C[GA][CT]GG
              819  CCATGG              
Eco81I        197  CCTTAGG              CC^T[ACGT]AGG
             1105  CCTCAGG             
MspA1I        925  CAGCGG               C[AC]G^C[GT]G
             1072  CAGCTG              
NcoI          272  CCATGG               C^CATGG
              819  CCATGG              
RsaI          400  GTAC                 GT^AC
              566  GTAC                
SacI          141  GAGCTC               GAGCT^C
              156  GAGCTC              
SfaNI         802  GCATC                GCATC(5/9)
             1129  GCATC               
TfiI         1017  GATTC                G^A[AT]TC
             1026  GAATC               
Tsp509I       472  AATT                 ^AATT
              862  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AluI        AspHI       BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        Sau3AI      
Sau96I      ScrFI       SfcI        StyI        TseI        
Tsp45I      TspRI       
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