Restriction map for pF1KSDA1737
 (Linear) (Six-base) MAPSORT of: KIAA1737.seq  Check: 4671  from: 1  to: 1197



KIAA1737.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     697    1197 
   Size:        697     500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1197 
   Size:        712     485
 
AleI CACnn'nnGTG

Cuts at:      0     428    1197 
   Size:        428     769
 
ApaI G_GGCC'C

Cuts at:      0    1104    1197 
   Size:       1104      93
 
ApoI r'AATT_y

Cuts at:      0     471    1197 
   Size:        471     726
 
BanII G_rGCy'C

Cuts at:      0      31     145     160    1104    1197 
   Size:         31     114      15     944      93

  Fragments arranged by size: 

                 944     114      93      31      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     221     584    1197 
   Size:         42     179     363     613

  Fragments arranged by size: 

                 613     363     179      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     747    1197 
   Size:        747     450
 
BclI T'GATC_A

Cuts at:      0    1182    1197 
   Size:       1182      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1108    1197 
   Size:       1108      89
 
BlpI GC'TnA_GC

Cuts at:      0     190    1197 
   Size:        190    1007
 
Bme1580I G_kGCm'C

Cuts at:      0     695    1104    1197 
   Size:        695     409      93

  Fragments arranged by size: 

                 695     409      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     758    1197 
   Size:        758     439
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     852     854    1197 
   Size:        852       2     343

  Fragments arranged by size: 

                 852     343       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     310     432    1009    1197 
   Size:        225      85     122     577     188

  Fragments arranged by size: 

                 577     225     188     122      85
 
BsaAI yAC'GTr

Cuts at:      0     750    1197 
   Size:        750     447
 
BsaHI Gr'CG_yC

Cuts at:      0     640    1197 
   Size:        640     557
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     252     282    1197 
   Size:        252      30     915

  Fragments arranged by size: 

                 915     252      30
 
BseYI C'CCAG_C

Cuts at:      0     320     652    1070    1188    1197 
   Size:        320     332     418     118       9

  Fragments arranged by size: 

                 418     332     320     118       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1197 
   Size:        571     626
 
BsiHKAI G_wGCw'C

Cuts at:      0     145     160     893    1197 
   Size:        145      15     733     304

  Fragments arranged by size: 

                 733     304     145      15
 
BsmI GAATG_Cn'

Cuts at:      0     255    1174    1197 
   Size:        255     919      23

  Fragments arranged by size: 

                 919     255      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     145     160     695     893    1104    1197 
   Size:         31     114      15     535     198     211      93

  Fragments arranged by size: 

                 535     211     198     114      93      31      15
 
BspHI T'CATG_A

Cuts at:      0     281    1197 
   Size:        281     916
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     697    1197 
   Size:        697     500
 
BsrFI r'CCGG_y

Cuts at:      0     732    1197 
   Size:        732     465
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     250     690    1197 
   Size:        250     440     507

  Fragments arranged by size: 

                 507     440     250
 
Bsu36I CC'TnA_GG

Cuts at:      0     198    1106    1197 
   Size:        198     908      91

  Fragments arranged by size: 

                 908     198      91
 
BtgI C'CryG_G

Cuts at:      0     272     819    1197 
   Size:        272     547     378

  Fragments arranged by size: 

                 547     378     272
 
BtsI GCAGTG_nn'

Cuts at:      0    1167    1197 
   Size:       1167      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     731    1197 
   Size:        731     466
 
EcoICRI GAG'CTC

Cuts at:      0     143     158    1197 
   Size:        143      15    1039

  Fragments arranged by size: 

                1039     143      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     310     432     712    1009    1197 
   Size:        225      85     122     280     297     188

  Fragments arranged by size: 

                 297     280     225     188     122      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     510    1100    1101    1197 
   Size:        510     590       1      96

  Fragments arranged by size: 

                 590     510      96       1
 
EcoRI G'AATT_C

Cuts at:      0     471    1197 
   Size:        471     726
 
EcoRV GAT'ATC

Cuts at:      0     912    1197 
   Size:        912     285
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270    1197 
   Size:        270     927
 
FspI TGC'GCA

Cuts at:      0     801    1197 
   Size:        801     396
 
HaeII r_GCGC'y

Cuts at:      0     197     659     948    1197 
   Size:        197     462     289     249

  Fragments arranged by size: 

                 462     289     249     197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     106     127     138     159     424     456    1197 
   Size:        106      21      11      21     265      32     741

  Fragments arranged by size: 

                 741     265     106      32      21      21      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1197 
   Size:        467     730
 
MslI CAynn'nnrTG

Cuts at:      0      67     428     876    1197 
   Size:         67     361     448     321

  Fragments arranged by size: 

                 448     361     321      67
 
MspA1I CmG'CkG

Cuts at:      0     927    1074    1197 
   Size:        927     147     123

  Fragments arranged by size: 

                 927     147     123
 
NcoI C'CATG_G

Cuts at:      0     272     819    1197 
   Size:        272     547     378

  Fragments arranged by size: 

                 547     378     272
 
NspI r_CATG'y

Cuts at:      0     189    1197 
   Size:        189    1008
 
PfoI T'CCnGG_A

Cuts at:      0     448    1197 
   Size:        448     749
 
PmlI CAC'GTG

Cuts at:      0     750    1197 
   Size:        750     447
 
PspOMI G'GGCC_C

Cuts at:      0    1100    1197 
   Size:       1100      97
 
PstI C_TGCA'G

Cuts at:      0     165     686     888    1127    1197 
   Size:        165     521     202     239      70

  Fragments arranged by size: 

                 521     239     202     165      70
 
PvuII CAG'CTG

Cuts at:      0    1074    1197 
   Size:       1074     123
 
SacI G_AGCT'C

Cuts at:      0     145     160    1197 
   Size:        145      15    1037

  Fragments arranged by size: 

                1037     145      15
 
SfcI C'TryA_G

Cuts at:      0     161     682     884    1123    1197 
   Size:        161     521     202     239      74

  Fragments arranged by size: 

                 521     239     202     161      74
 
StyI C'CwwG_G

Cuts at:      0     272     519     608     819    1007    1197 
   Size:        272     247      89     211     188     190

  Fragments arranged by size: 

                 272     247     211     190     188      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     666    1197 
   Size:        666     531
 
TatI w'GTAC_w

Cuts at:      0     565    1197 
   Size:        565     632
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     747    1197 
   Size:        747     450
 
XmnI GAAnn'nnTTC

Cuts at:      0     253    1197 
   Size:        253     944
 

 Enzymes that do cut:

     AarI     AcuI     AleI     ApaI     ApoI    BanII     BbsI    BciVI
     BclI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI    BsaAI
    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrFI   BstAPI   Bsu36I     BtgI     BtsI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
     MmeI     MslI   MspA1I     NcoI     NspI     PfoI     PmlI   PspOMI
     PstI    PvuII     SacI     SfcI     StyI    TaqII     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaWI    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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