Restriction map for pF1KSDA1724
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       BalI        BbsI        BcgI        BfrI        
BglI        BglII       BpmI        BpuAI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstUI       BstXI       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoRI       EcoRV       FauI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        MwoI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AcyI          144  GGCGCC               G[GA]^CG[CT]C
AflIII        910  ACATGT               A^C[GA][CT]GT
AocI          356  CCTTAGG              CC^T[ACGT]AGG
Asp700I       338  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        1005  CCTAGG               C^CTAGG
BamHI         827  GGATCC               G^GATCC
BanI          144  GGCGCC               G^G[CT][GA]CC
BanII          21  GAGCCC               G[GA]GC[CT]^C
BbeI          144  GGCGCC               GGCGC^C
BbrPI         250  CACGTG               CAC^GTG
BbvCI         230  CCTCAGC              CCTCAGC(-5/-2)
BciVI          69  GGATAC               GTATCC(6/5)
BclI          372  TGATCA               T^GATCA
BlnI         1005  CCTAGG               C^CTAGG
BlpI         1104  GCTGAGC              GC^T[ACGT]AGC
BmyI           21  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I     1104  GCTGAGC              GC^T[ACGT]AGC
BsaBI          70  GATACCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         144  GGCGCC               G[GA]^CG[CT]C
BsaI          656  GAGACC               GGTCTC(1/5)
BsmAI         656  GAGAC                GTCTC(1/5)
BsmFI         507  GTCCC                GGGAC(10/14)
BsmI         1031  GCATTC               GAATGC(1/-1)
Bsp1286I       21  GAGCCC               G[AGT]GC[ACT]^C
BspLU11I      910  ACATGT               A^CATGT
BsrDI         670  GCAATG               GCAATG(2/0)
Bst1107I     1041  GTATAC               GTA^TAC
BstEII        836  GGTCACC              G^GT[ACGT]ACC
BstPI         836  GGTCACC              G^GT[ACGT]ACC
BstYI         827  GGATCC               [GA]^GATC[CT]
BstZ17I      1041  GTATAC               GTA^TAC
Bsu36I        356  CCTTAGG              CC^T[ACGT]AGG
CelII        1104  GCTGAGC              GC^T[ACGT]AGC
CfoI          145  GCGC                 GCG^C
Cfr13I       1068  GGCCC                G^G[ACGT]CC
DsaI          547  CCATGG               C^C[GA][CT]GG
Eco81I        356  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       836  GGTCACC              G^GT[ACGT]ACC
EcoT22I        97  ATGCAT               ATGCA^T
FbaI          372  TGATCA               T^GATCA
HaeII         144  GGCGCC               [GA]GCGC^[CT]
HhaI          145  GCGC                 GCG^C
Hin1I         144  GGCGCC               G[GA]^CG[CT]C
HinP1I        145  GCGC                 G^CGC
KasI          144  GGCGCC               G^GCGCC
MamI           70  GATACCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          827  GGATCC               [GA]^GATC[CT]
MslI          666  CACTGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          144  GGCGCC               GG^CGCC
NcoI          547  CCATGG               C^CATGG
NsiI           97  ATGCAT               ATGCA^T
NspI          910  ACATGT               [GA]CATG^[CT]
PleI          699  GACTC                GAGTC(4/5)
PmaCI         250  CACGTG               CAC^GTG
PmlI          250  CACGTG               CAC^GTG
PstI          599  CTGCAG               CTGCA^G
Sau96I       1068  GGCCC                G^G[ACGT]CC
SexAI         237  ACCAGGT              A^CC[AT]GGT
SfoI          144  GGCGCC               GGC^GCC
StuI          617  AGGCCT               AGG^CCT
TfiI          534  GATTC                G^A[AT]TC
XbaI          306  TCTAGA               T^CTAGA
XhoII         827  GGATCC               [GA]^GATC[CT]
XmnI          338  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BcnI           24  CCCGG                CC^[GC]GG
              944  CCCGG               
BsaAI          16  TACGTG               [CT]AC^GT[GA]
              250  CACGTG              
BsaJI         547  CCATGG               C^C[ACGT][ACGT]GG
             1005  CCTAGG              
BsiYI         474  CCTGCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              543  CCTGCCATGGG         
BslI          474  CCTGCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              543  CCTGCCATGGG         
EcoT14I       547  CCATGG               C^C[AT][AT]GG
             1005  CCTAGG              
HapII          25  CCGG                 C^CGG
              945  CCGG                
HinfI         534  GATTC                G^A[ACGT]TC
              699  GACTC               
HpaII          25  CCGG                 C^CGG
              945  CCGG                
HphI          570  GGTGA                GGTGA(8/7)
              838  TCACC               
MspI           25  CCGG                 C^CGG
              945  CCGG                
NciI           24  CCCGG                CC^[GC]GG
              944  CCCGG               
SfaNI          99  GCATC                GCATC(5/9)
              281  GCATC               
StyI          547  CCATGG               C^C[AT][AT]GG
             1005  CCTAGG              
XcmI          952  CCAACTTTGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1071  CCACATCCATTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AccI        AfaI        AluI        AlwI        BbvI        
BfaI        BsrI        BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MvaI        NdeII       
NlaIII      NlaIV       PvuII       RsaI        Sau3AI      
ScrFI       SfcI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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