Restriction map for pF1KSDA1724
 (Linear) (Six-base) MAPSORT of: KIAA1724.seq  Check: 5338  from: 1  to: 1158



KIAA1724.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     587     817    1042    1158 
   Size:        587     230     225     116

  Fragments arranged by size: 

                 587     230     225     116
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827    1158 
   Size:        827     331
 
AflIII A'CryG_T

Cuts at:      0     910    1158 
   Size:        910     248
 
AvrII C'CTAG_G

Cuts at:      0    1005    1158 
   Size:       1005     153
 
BamHI G'GATC_C

Cuts at:      0     827    1158 
   Size:        827     331
 
BanI G'GyrC_C

Cuts at:      0     144    1158 
   Size:        144    1014
 
BanII G_rGCy'C

Cuts at:      0      25    1158 
   Size:         25    1133
 
BbeI G_GCGC'C

Cuts at:      0     148    1158 
   Size:        148    1010
 
BbvCI CC'TCA_GC

Cuts at:      0     231    1158 
   Size:        231     927
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      63    1158 
   Size:         63    1095
 
BclI T'GATC_A

Cuts at:      0     372    1158 
   Size:        372     786
 
BfrBI ATG'CAT

Cuts at:      0      99    1158 
   Size:         99    1059
 
BlpI GC'TnA_GC

Cuts at:      0    1105    1158 
   Size:       1105      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270     522    1158 
   Size:        270     252     636

  Fragments arranged by size: 

                 636     270     252
 
Bpu10I CC'TnA_GC

Cuts at:      0     231    1158 
   Size:        231     927
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     650    1158 
   Size:        650     508
 
BsaAI yAC'GTr

Cuts at:      0      18     252    1158 
   Size:         18     234     906

  Fragments arranged by size: 

                 906     234      18
 
BsaBI GATnn'nnATC

Cuts at:      0      74    1158 
   Size:         74    1084
 
BsaHI Gr'CG_yC

Cuts at:      0     145    1158 
   Size:        145    1013
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1076    1106    1158 
   Size:       1076      30      52

  Fragments arranged by size: 

                1076      52      30
 
BsmI GAATG_Cn'

Cuts at:      0    1031    1158 
   Size:       1031     127
 
Bsp1286I G_dGCh'C

Cuts at:      0      25    1158 
   Size:         25    1133
 
BsrDI GCAATG_nn'

Cuts at:      0     677    1158 
   Size:        677     481
 
BstEII G'GTnAC_C

Cuts at:      0     836    1158 
   Size:        836     322
 
BstYI r'GATC_y

Cuts at:      0     827    1158 
   Size:        827     331
 
BstZ17I GTA'TAC

Cuts at:      0    1043    1158 
   Size:       1043     115
 
Bsu36I CC'TnA_GG

Cuts at:      0     357    1158 
   Size:        357     801
 
BtgI C'CryG_G

Cuts at:      0     547    1158 
   Size:        547     611
 
BtsI GCAGTG_nn'

Cuts at:      0     665    1158 
   Size:        665     493
 
EarI CTCTTCn'nnn_

Cuts at:      0     429     707     985    1158 
   Size:        429     278     278     173

  Fragments arranged by size: 

                 429     278     278     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827    1158 
   Size:        827     331
 
HaeII r_GCGC'y

Cuts at:      0     148    1158 
   Size:        148    1010
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408    1158 
   Size:        376      32     750

  Fragments arranged by size: 

                 750     376      32
 
KasI G'GCGC_C

Cuts at:      0     144    1158 
   Size:        144    1014
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     867     976    1158 
   Size:        867     109     182

  Fragments arranged by size: 

                 867     182     109
 
MslI CAynn'nnrTG

Cuts at:      0     670    1158 
   Size:        670     488
 
MspA1I CmG'CkG

Cuts at:      0      33     918    1104    1158 
   Size:         33     885     186      54

  Fragments arranged by size: 

                 885     186      54      33
 
NarI GG'CG_CC

Cuts at:      0     145    1158 
   Size:        145    1013
 
NcoI C'CATG_G

Cuts at:      0     547    1158 
   Size:        547     611
 
NsiI A_TGCA'T

Cuts at:      0     101    1158 
   Size:        101    1057
 
NspI r_CATG'y

Cuts at:      0     914    1158 
   Size:        914     244
 
PciI A'CATG_T

Cuts at:      0     910    1158 
   Size:        910     248
 
PmlI CAC'GTG

Cuts at:      0     252    1158 
   Size:        252     906
 
PstI C_TGCA'G

Cuts at:      0     603    1158 
   Size:        603     555
 
PvuII CAG'CTG

Cuts at:      0      33     918    1104    1158 
   Size:         33     885     186      54

  Fragments arranged by size: 

                 885     186      54      33
 
SexAI A'CCwGG_T

Cuts at:      0     237    1158 
   Size:        237     921
 
SfcI C'TryA_G

Cuts at:      0     116     599     818    1158 
   Size:        116     483     219     340

  Fragments arranged by size: 

                 483     340     219     116
 
SfoI GGC'GCC

Cuts at:      0     146    1158 
   Size:        146    1012
 
StuI AGG'CCT

Cuts at:      0     619    1158 
   Size:        619     539
 
StyI C'CwwG_G

Cuts at:      0     547    1005    1158 
   Size:        547     458     153

  Fragments arranged by size: 

                 547     458     153
 
TatI w'GTAC_w

Cuts at:      0      50      56     907    1158 
   Size:         50       6     851     251

  Fragments arranged by size: 

                 851     251      50       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1076    1108    1158 
   Size:       1076      32      50

  Fragments arranged by size: 

                1076      50      32
 
XbaI T'CTAG_A

Cuts at:      0     306    1158 
   Size:        306     852
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     959    1078    1158 
   Size:        959     119      80

  Fragments arranged by size: 

                 959     119      80
 
XmnI GAAnn'nnTTC

Cuts at:      0     342    1158 
   Size:        342     816
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BlpI     BmrI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI     BsmI Bsp1286I    BsrDI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     EarI  Eco57MI    HaeII
    Hin4I     KasI     MmeI     MslI   MspA1I     NarI     NcoI     NsiI
     NspI     PciI     PmlI     PstI    PvuII    SexAI     SfcI     SfoI
     StuI     StyI     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI     BbsI     BcgI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII  Tth111I     XhoI     XmaI     ZraI
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