Restriction map for pF1KSDA1722
 (Linear) (Six-base) MAPSORT of: KIAA1722.seq  Check: 9442  from: 1  to: 4497



KIAA1722.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     926    1415    4497 
   Size:        926     489    3082

  Fragments arranged by size: 

                3082     926     489
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2167    2395    3507    4497 
   Size:       2167     228    1112     990

  Fragments arranged by size: 

                2167    1112     990     228
 
AflII C'TTAA_G

Cuts at:      0     651    2697    4069    4497 
   Size:        651    2046    1372     428

  Fragments arranged by size: 

                2046    1372     651     428
 
AleI CACnn'nnGTG

Cuts at:      0     727    4497 
   Size:        727    3770
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     352     745    3481    4497 
   Size:        352     393    2736    1016

  Fragments arranged by size: 

                2736    1016     393     352
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4268    4300    4497 
   Size:       4268      32     197

  Fragments arranged by size: 

                4268     197      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1401    4461    4497 
   Size:       1401    3060      36

  Fragments arranged by size: 

                3060    1401      36
 
ApaI G_GGCC'C

Cuts at:      0    3554    4497 
   Size:       3554     943
 
ApoI r'AATT_y

Cuts at:      0      29     292    1123    1471    1761    4087    4497 
   Size:         29     263     831     348     290    2326     410

  Fragments arranged by size: 

                2326     831     410     348     290     263      29
 
AseI AT'TA_AT

Cuts at:      0    2098    2237    4497 
   Size:       2098     139    2260

  Fragments arranged by size: 

                2260    2098     139
 
AvaI C'yCGr_G

Cuts at:      0     132    1826    2912    4497 
   Size:        132    1694    1086    1585

  Fragments arranged by size: 

                1694    1585    1086     132
 
BanI G'GyrC_C

Cuts at:      0    3744    3955    4336    4497 
   Size:       3744     211     381     161

  Fragments arranged by size: 

                3744     381     211     161
 
BanII G_rGCy'C

Cuts at:      0    1854    3554    4331    4497 
   Size:       1854    1700     777     166

  Fragments arranged by size: 

                1854    1700     777     166
 
BbeI G_GCGC'C

Cuts at:      0    4340    4497 
   Size:       4340     157
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2999    3206    3638    4104    4497 
   Size:       2999     207     432     466     393

  Fragments arranged by size: 

                2999     466     432     393     207
 
BbvCI CC'TCA_GC

Cuts at:      0     172     684    1267    4497 
   Size:        172     512     583    3230

  Fragments arranged by size: 

                3230     583     512     172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     105     139    2851    2885    4497 
   Size:        105      34    2712      34    1612

  Fragments arranged by size: 

                2712    1612     105      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1215    4497 
   Size:       1215    3282
 
BclI T'GATC_A

Cuts at:      0     303    3897    4497 
   Size:        303    3594     600

  Fragments arranged by size: 

                3594     600     303
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2599    4497 
   Size:       2599    1898
 
BglII A'GATC_T

Cuts at:      0    2795    4497 
   Size:       2795    1702
 
BlpI GC'TnA_GC

Cuts at:      0    1529    4497 
   Size:       1529    2968
 
Bme1580I G_kGCm'C

Cuts at:      0    3323    3554    3749    4297    4497 
   Size:       3323     231     195     548     200

  Fragments arranged by size: 

                3323     548     231     200     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     234    3369    4437    4497 
   Size:        234    3135    1068      60

  Fragments arranged by size: 

                3135    1068     234      60
 
BmtI G_CTAG'C

Cuts at:      0    2177    2318    4497 
   Size:       2177     141    2179

  Fragments arranged by size: 

                2179    2177     141
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4203    4497 
   Size:       4203     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     442     652     890    1339    1423    1537    3067 
   Size:        268     174     210     238     449      84     114    1530

Cuts at:   3067    3752    4497 
   Size:        685     745

  Fragments arranged by size: 

                1530     745     685     449     268     238     210     174
                 114      84
 
Bpu10I CC'TnA_GC

Cuts at:      0     172     684    1081    1267    4497 
   Size:        172     512     397     186    3230

  Fragments arranged by size: 

                3230     512     397     186     172
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     772     967    2720    2812    4497 
   Size:        517     255     195    1753      92    1685

  Fragments arranged by size: 

                1753    1685     517     255     195      92
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     673    1147    4209    4497 
   Size:        673     474    3062     288

  Fragments arranged by size: 

                3062     673     474     288
 
BsaAI yAC'GTr

Cuts at:      0    2611    4497 
   Size:       2611    1886
 
BsaBI GATnn'nnATC

Cuts at:      0    2339    4497 
   Size:       2339    2158
 
BsaHI Gr'CG_yC

Cuts at:      0    1957    4238    4337    4497 
   Size:       1957    2281      99     160

  Fragments arranged by size: 

                2281    1957     160      99
 
BsaWI w'CCGG_w

Cuts at:      0    1748    3512    3557    4497 
   Size:       1748    1764      45     940

  Fragments arranged by size: 

                1764    1748     940      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     148     178     182     212    1589    1619    2509    2539 
   Size:        148      30       4      30    1377      30     890      30

Cuts at:   2539    3298    3328    4042    4072    4497 
   Size:        759      30     714      30     425

  Fragments arranged by size: 

                1377     890     759     714     425     148      30      30
                  30      30      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2132    2740    3583    3657    4497 
   Size:       2132     608     843      74     840

  Fragments arranged by size: 

                2132     843     840     608      74
 
BseYI C'CCAG_C

Cuts at:      0     414    1534    1678    1686    2055    2780    3492    3546 
   Size:        414    1120     144       8     369     725     712      54

Cuts at:   3546    4345    4472    4497 
   Size:        799     127      25

  Fragments arranged by size: 

                1120     799     725     712     414     369     144     127
                  54      25       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2405    2467    2650    4497 
   Size:       2405      62     183    1847

  Fragments arranged by size: 

                2405    1847     183      62
 
BsiEI CG_ry'CG

Cuts at:      0    1741    4047    4497 
   Size:       1741    2306     450

  Fragments arranged by size: 

                2306    1741     450
 
BsiHKAI G_wGCw'C

Cuts at:      0    1715    1854    3835    4497 
   Size:       1715     139    1981     662

  Fragments arranged by size: 

                1981    1715     662     139
 
Bsp1286I G_dGCh'C

Cuts at:      0    1715    1854    3323    3554    3749    3835    4297    4331 
   Size:       1715     139    1469     231     195      86     462      34

Cuts at:   4331    4497 
   Size:        166

  Fragments arranged by size: 

                1715    1469     462     231     195     166     139      86
                  34
 
BspEI T'CCGG_A

Cuts at:      0    3557    4497 
   Size:       3557     940
 
BspHI T'CATG_A

Cuts at:      0    4169    4497 
   Size:       4169     328
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2951    4497 
   Size:       2951    1546
 
BsrBI CCG'CTC

Cuts at:      0     242     621    2928    4497 
   Size:        242     379    2307    1569

  Fragments arranged by size: 

                2307    1569     379     242
 
BsrFI r'CCGG_y

Cuts at:      0    2060    2546    4497 
   Size:       2060     486    1951

  Fragments arranged by size: 

                2060    1951     486
 
BsrGI T'GTAC_A

Cuts at:      0    1858    3470    4497 
   Size:       1858    1612    1027

  Fragments arranged by size: 

                1858    1612    1027
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4059    4461    4497 
   Size:       4059     402      36

  Fragments arranged by size: 

                4059     402      36
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588    3193    4497 
   Size:        588    2605    1304

  Fragments arranged by size: 

                2605    1304     588
 
BstYI r'GATC_y

Cuts at:      0     691    2373    2795    3162    4497 
   Size:        691    1682     422     367    1335

  Fragments arranged by size: 

                1682    1335     691     422     367
 
BstZ17I GTA'TAC

Cuts at:      0    1416    4497 
   Size:       1416    3081
 
Bsu36I CC'TnA_GG

Cuts at:      0    1909    3233    3664    4497 
   Size:       1909    1324     431     833

  Fragments arranged by size: 

                1909    1324     833     431
 
BtgI C'CryG_G

Cuts at:      0    1159    1974    3269    3637    3947    4361    4497 
   Size:       1159     815    1295     368     310     414     136

  Fragments arranged by size: 

                1295    1159     815     414     368     310     136
 
BtsI GCAGTG_nn'

Cuts at:      0    3001    4297    4459    4497 
   Size:       3001    1296     162      38

  Fragments arranged by size: 

                3001    1296     162      38
 
DraI TTT'AAA

Cuts at:      0    1308    1590    2814    4497 
   Size:       1308     282    1224    1683

  Fragments arranged by size: 

                1683    1308    1224     282
 
DraIII CAC_nnn'GTG

Cuts at:      0     279    4497 
   Size:        279    4218
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3607    3994    4497 
   Size:       3607     387     503

  Fragments arranged by size: 

                3607     503     387
 
EaeI y'GGCC_r

Cuts at:      0    1738    1835    3700    4497 
   Size:       1738      97    1865     797

  Fragments arranged by size: 

                1865    1738     797      97
 
EagI C'GGCC_G

Cuts at:      0    1738    4497 
   Size:       1738    2759
 
EarI CTCTTCn'nnn_

Cuts at:      0    1145    1352    2032    2888    3958    4497 
   Size:       1145     207     680     856    1070     539

  Fragments arranged by size: 

                1145    1070     856     680     539     207
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3104    4497 
   Size:       3104    1393
 
EcoICRI GAG'CTC

Cuts at:      0    1852    4497 
   Size:       1852    2645
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     442     652     890    1339    1423    1537    2167 
   Size:        268     174     210     238     449      84     114     630

Cuts at:   2167    2395    3067    3507    3752    4497 
   Size:        228     672     440     245     745

  Fragments arranged by size: 

                 745     672     630     449     440     268     245     238
                 228     210     174     114      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4072    4138    4497 
   Size:       4072      66     359

  Fragments arranged by size: 

                4072     359      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3550    4497 
   Size:       3550     947
 
EcoRI G'AATT_C

Cuts at:      0      29    1123    4497 
   Size:         29    1094    3374

  Fragments arranged by size: 

                3374    1094      29
 
EcoRV GAT'ATC

Cuts at:      0    2964    4497 
   Size:       2964    1533
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1837    2307    4497 
   Size:       1837     470    2190

  Fragments arranged by size: 

                2190    1837     470
 
HaeII r_GCGC'y

Cuts at:      0    2675    4340    4497 
   Size:       2675    1665     157

  Fragments arranged by size: 

                2675    1665     157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180    1710    1742    1836    1868    2912    2944 
   Size:        148      32    1530      32      94      32    1044      32

Cuts at:   2944    3049    3081    3406    3438    3565    3597    3864    3896 
   Size:        105      32     325      32     127      32     267      32

Cuts at:   3896    4497 
   Size:        601

  Fragments arranged by size: 

                1530    1044     601     325     267     148     127     105
                  94      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2109    2164    4497 
   Size:       2109      55    2333

  Fragments arranged by size: 

                2333    2109      55
 
HindIII A'AGCT_T

Cuts at:      0     370    4497 
   Size:        370    4127
 
HpaI GTT'AAC

Cuts at:      0    2109    4497 
   Size:       2109    2388
 
KasI G'GCGC_C

Cuts at:      0    4336    4497 
   Size:       4336     161
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3404    4497 
   Size:       3404    1093
 
MscI TGG'CCA

Cuts at:      0    1837    4497 
   Size:       1837    2660
 
MslI CAynn'nnrTG

Cuts at:      0      49     586     727    3191    4210    4497 
   Size:         49     537     141    2464    1019     287

  Fragments arranged by size: 

                2464    1019     537     287     141      49
 
MspA1I CmG'CkG

Cuts at:      0     382     414    1682    3389    3639    3857    4497 
   Size:        382      32    1268    1707     250     218     640

  Fragments arranged by size: 

                1707    1268     640     382     250     218      32
 
NaeI GCC'GGC

Cuts at:      0    2062    4497 
   Size:       2062    2435
 
NarI GG'CG_CC

Cuts at:      0    4337    4497 
   Size:       4337     160
 
NcoI C'CATG_G

Cuts at:      0    1159    3269    4497 
   Size:       1159    2110    1228

  Fragments arranged by size: 

                2110    1228    1159
 
NdeI CA'TA_TG

Cuts at:      0    2855    4497 
   Size:       2855    1642
 
NgoMIV G'CCGG_C

Cuts at:      0    2060    4497 
   Size:       2060    2437
 
NheI G'CTAG_C

Cuts at:      0    2173    2314    4497 
   Size:       2173     141    2183

  Fragments arranged by size: 

                2183    2173     141
 
NspI r_CATG'y

Cuts at:      0    1676    4011    4066    4497 
   Size:       1676    2335      55     431

  Fragments arranged by size: 

                2335    1676     431      55
 
PasI CC'CwG_GG

Cuts at:      0    3585    4497 
   Size:       3585     912
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     675    1401    3912    4497 
   Size:        675     726    2511     585

  Fragments arranged by size: 

                2511     726     675     585
 
PfoI T'CCnGG_A

Cuts at:      0    1341    2273    2684    4497 
   Size:       1341     932     411    1813

  Fragments arranged by size: 

                1813    1341     932     411
 
PsiI TTA'TAA

Cuts at:      0    3593    4497 
   Size:       3593     904
 
PspOMI G'GGCC_C

Cuts at:      0    3550    4497 
   Size:       3550     947
 
PstI C_TGCA'G

Cuts at:      0     341    1236    2948    3887    4497 
   Size:        341     895    1712     939     610

  Fragments arranged by size: 

                1712     939     895     610     341
 
PvuII CAG'CTG

Cuts at:      0     382     414    1682    4497 
   Size:        382      32    1268    2815

  Fragments arranged by size: 

                2815    1268     382      32
 
SacI G_AGCT'C

Cuts at:      0    1854    4497 
   Size:       1854    2643
 
SacII CC_GC'GG

Cuts at:      0    3640    4497 
   Size:       3640     857
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3958    4497 
   Size:       3958     539
 
SbfI CC_TGCA'GG

Cuts at:      0    2948    4497 
   Size:       2948    1549
 
SexAI A'CCwGG_T

Cuts at:      0    2297    4497 
   Size:       2297    2200
 
SfcI C'TryA_G

Cuts at:      0     337     812     964    1232    2675    2769    2880    2944 
   Size:        337     475     152     268    1443      94     111      64

Cuts at:   2944    3883    4497 
   Size:        939     614

  Fragments arranged by size: 

                1443     939     614     475     337     268     152     111
                  94      64
 
SfoI GGC'GCC

Cuts at:      0    4338    4497 
   Size:       4338     159
 
SmaI CCC'GGG

Cuts at:      0    2914    4497 
   Size:       2914    1583
 
SmlI C'TyrA_G

Cuts at:      0     532     651     787     982    2697    2735    2791    4069 
   Size:        532     119     136     195    1715      38      56    1278

Cuts at:   4069    4497 
   Size:        428

  Fragments arranged by size: 

                1715    1278     532     428     195     136     119      56
                  38
 
SphI G_CATG'C

Cuts at:      0    4066    4497 
   Size:       4066     431
 
SspI AAT'ATT

Cuts at:      0    1495    3651    4497 
   Size:       1495    2156     846

  Fragments arranged by size: 

                2156    1495     846
 
StuI AGG'CCT

Cuts at:      0     901    2593    2878    4497 
   Size:        901    1692     285    1619

  Fragments arranged by size: 

                1692    1619     901     285
 
StyI C'CwwG_G

Cuts at:      0    1159    1351    3180    3269    3289    3336    4497 
   Size:       1159     192    1829      89      20      47    1161

  Fragments arranged by size: 

                1829    1161    1159     192      89      47      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85    1540    1673    2819    3349    4449    4497 
   Size:         85    1455     133    1146     530    1100      48

  Fragments arranged by size: 

                1455    1146    1100     530     133      85      48
 
TatI w'GTAC_w

Cuts at:      0     395    1858    3470    3809    4497 
   Size:        395    1463    1612     339     688

  Fragments arranged by size: 

                1612    1463     688     395     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214    4497 
   Size:        182      32    4283

  Fragments arranged by size: 

                4283     182      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     922    4497 
   Size:        922    3575
 
XbaI T'CTAG_A

Cuts at:      0    1062    3426    4497 
   Size:       1062    2364    1071

  Fragments arranged by size: 

                2364    1071    1062
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      94     702    3276    3525    4497 
   Size:         94     608    2574     249     972

  Fragments arranged by size: 

                2574     972     608     249      94
 
XmaI C'CCGG_G

Cuts at:      0    2912    4497 
   Size:       2912    1585
 
XmnI GAAnn'nnTTC

Cuts at:      0    1202    1472    1948    4497 
   Size:       1202     270     476    2549

  Fragments arranged by size: 

                2549    1202     476     270
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NspI     PasI    PflMI     PfoI
     PsiI   PspOMI     PstI    PvuII     SacI    SacII     SapI     SbfI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI    BfrBI    BmgBI
    BsiWI     BsmI    BsmBI    BsrDI   BssHII    BssSI    BstBI   BstEII
    BtgZI     ClaI    CspCI     FseI     FspI    FspAI     KpnI     MfeI
     MluI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SwaI     XhoI     ZraI
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