Restriction map for pF1KSDA1720
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       BalI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BsaBI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MslI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII        644  TCCGGA               T^CCGGA
AfaI          408  GTAC                 GT^AC
AlwNI         700  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       399  AGCGCT               AGC^GCT
AspI         1075  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1013  TGCGCA               TGC^GCA
AvrII          82  CCTAGG               C^CTAGG
BbrPI         501  CACGTG               CAC^GTG
BbsI          636  GAAGAC               GAAGAC(2/6)
BbvCI         610  GCTGAGG              CCTCAGC(-5/-2)
BlnI           82  CCTAGG               C^CTAGG
BpuAI         636  GAAGAC               GAAGAC(2/6)
BsaAI         501  CACGTG               [CT]AC^GT[GA]
BsaI           34  GAGACC               GGTCTC(1/5)
BsaWI         644  TCCGGA               [AT]^CCGG[AT]
BseAI         644  TCCGGA               T^CCGGA
BspEI         644  TCCGGA               T^CCGGA
BstAPI        507  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI         1092  CGGCCA               [CT]^GGCC[GA]
Eco47III      399  AGCGCT               AGC^GCT
FspI         1013  TGCGCA               TGC^GCA
KspI          825  CCGCGG               CCGC^GG
MroI          644  TCCGGA               T^CCGGA
NspI          425  GCATGC               [GA]CATG^[CT]
PaeR7I        440  CTCGAG               C^TCGAG
PflFI        1075  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         501  CACGTG               CAC^GTG
PmlI          501  CACGTG               CAC^GTG
PpuMI         888  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         238  CAGCTG               CAG^CTG
RsaI          408  GTAC                 GT^AC
SacII         825  CCGCGG               CCGC^GG
SexAI         224  ACCAGGT              A^CC[AT]GGT
SphI          425  GCATGC               GCATG^C
StuI         1065  AGGCCT               AGG^CCT
TfiI          810  GATTC                G^A[AT]TC
Tsp509I       140  AATT                 ^AATT
TspRI         129  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I      1075  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          440  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

ApaI          852  GGGCCC               GGGCC^C
              967  GGGCCC              
BamHI         168  GGATCC               G^GATCC
              269  GGATCC              
BbeI          821  GGCGCC               GGCGC^C
             1121  GGCGCC              
BfaI           83  CTAG                 C^TAG
              173  CTAG                
BsmFI          79  GTCCC                GGGAC(10/14)
              144  GTCCC               
BstF5I         72  GGATG                GGATG(2/0)
              243  GGATG               
EarI          100  GAAGAG               CTCTTC(1/4)
              553  GAAGAG              
EcoT14I        82  CCTAGG               C^C[AT][AT]GG
              722  CCAAGG              
FokI           72  GGATG                GGATG(9/13)
              243  GGATG               
KasI          821  GGCGCC               G^GCGCC
             1121  GGCGCC              
Ksp632I       100  GAAGAG               CTCTTC(1/4)
              553  GAAGAG              
MaeI           83  CTAG                 C^TAG
              173  CTAG                
MaeII         502  ACGT                 A^CGT
             1079  ACGT                
NarI          821  GGCGCC               GG^CGCC
             1121  GGCGCC              
PstI          631  CTGCAG               CTGCA^G
             1033  CTGCAG              
SacI            7  GAGCTC               GAGCT^C
              679  GAGCTC              
SapI          100  GAAGAGC              GCTCTTC(1/4)
              553  GAAGAGC             
SfaNI         513  GATGC                GCATC(5/9)
              326  GCATC               
SfoI          821  GGCGCC               GGC^GCC
             1121  GGCGCC              
SmlI          440  CTCGAG               C^T[CT][GA]AG
              682  CTCAAG              
StyI           82  CCTAGG               C^C[AT][AT]GG
              722  CCAAGG              
Tsp45I        589  GTGAC                ^GT[GC]AC
              727  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        TthHB8I     Van91I      
XhoII       
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